A researcher wishes to increase the half-life of a particular mRNA that she is transfecting into a eukaryotic cell line. How should this researcher accomplish this goal?

a. Inhibit RNA polymerase I.
b. Increase the length of the poly(A) tail of the mRNA prior to transfection.
c. Inhibit the spliceosomes in the cell.
d. Increase the length of the 5'; cap of the mRNA prior to transfection.

Answers

Answer 1

Answer:

b. Increase the length of the poly(A) tail of the mRNA prior to transfection.

Explanation:

Polyadenylation is the addition of a long chain of adenine (A) nucleotides at the 3' end during RNA processing. This resultant chain is known as poly(A) tail. A poly-A tail generally has a size of 100-250 residues long. The poly(A) tail increases the stability of the messenger mRNA prior to the translation by slowing down its degradation rate over time. The process of polyadenylation enables RNA transcripts to be exported from the nucleus in order to be translated by ribosomes in the cytoplasm.


Related Questions

5.The chemical bonds between 2 oxygen atoms are polar covalent bonds.
Select one:
O True
O False

Answers

Answer:

False

Explanation

There is no substantial difference in electronegativities that would create a polar bond... electrons would NOT be unevenly distributed in O2, meaning that the molecule has a nonpolar covalent bond

It is False have a good day

Approximately how many base pairs are improperly matched before being repaired by the DNA polymerase?

a. 1 in 10,000
b. 1 in 100,000
c. 1 in 1,000,000
d. 1 in 10,000,000
e. 1 in a billion

Answers

Nucleic acids are made of fundamental units called base pairs along with sugar and phosphate groups.

The base pairs are joined unitedly by a hydrogen bond.

The correct answer is:

Option b. 1 in 100,000

This can be explained as:

In the eukaryotic cells, there is a chance once in 10⁵ polymerized nucleotides to have an error.

The errors are repaired by the DNA repair mechanism and the correct nucleotide is added.

Therefore, once in 10,000 base pairs are matched wrongly before being repaired by DNA polymerase.

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How would the structure of bone differ if it contained no minerals?

Answers

It would lose its firm like quality and be more breakable since the mineral component of a bone is composed of hydroxyapatite and the adult bone’s mass is 65% of hydroxyapatite. You can visualize what would happen if more than half of the bone’s mass were to be taken away

Where does aerobic respiration occur in the cell?
Ribosome

Cytoplasm

Mitochondria

cell membrane

Answers

Answer:

Mitochondria

Explanation:

Aerobic respiration has most of the characteristic of eukaryotic cells. So, when they have enough oxygen it happens within the mitochondria.

Which effect could a mutation in mRNA have on the production of proteins?

Answers

mRNA is the messenger molecule, which transcripts the information present in the DNA and then translates it to make the polypeptides. The mutations on mRNA can affect the synthesis of proteins.

The effect of mutation on mRNA:

Point mutations refer to the insertion or deletion of a single nucleotide from the sequence.

The mutations can seriously affect the organism and the functioning of the protein formation.

The point mutation leads to alteration of the amino acid sequence, which may lead to dangerous diseases or malfunctioning.

Thus, the mutations in the mRNA can result in serious alteration of the amino acid sequence of an organism.

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Which condition is important for a temperature inversion?

two layers of warm air

two layers of warm air, one on top of the other

a layer of warm air over a
layer of cold air

a layer of cold air over one of warm air

Answers

Answer:

the answer is that"a layer of warm air over a layer of cold air" which can be caused by factors like formation of fronts, scattering, advection.

The condition important for a temperature inversion is a layer of warm air over a layer of cold air. Hence option C is correct.

What is temperature?

Temperature is defined as any scale used to represent how hot or cold something is, including the Celsius and Fahrenheit scales. Food, beverages, agriculture, and medical care for both people and animals all depend heavily on temperature. Temperature also frequently affects our general health in a number of ways. In facilities used for medical cold storage, maintaining optimum temperatures is crucial.

The usual behavior of temperature in the troposphere is reversed by a phenomenon known as temperature inversion. A layer of warm air sits on top of the layer of cold air beneath this meteorological occurrence. It is brought on by static atmospheric circumstances, while it can also be brought on by air moving horizontally or vertically.

Thus, the condition important for a temperature inversion is a layer of warm air over a layer of cold air. Hence option C is correct.

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Breakdown of dead/decomposing materials into ammonia?

Answers

Answer:

Breakdown of dead materials into ammonia occurs due to decomposers.

Explanation:

Decomposers are the organisms which are responsible for the breakdown of dead bodies of plants and animals produces ammonia by consuming the nitrogen present in that dead body of plant and animals through the process of ammonification. The ammonium produced is then converted into nitrates by the nitrifying bacteria and this nitrates is used by the plants for making amino acids and enzymes.

Humans have 23
chromosome pairs.
COMPLETE
have two X chromosomes, while
have one X chromosome and one Y
chromosome.
DONE

Answers

Answer:

Females have two X chromosomes, while males have one X chromosome and one Y chromosome.

Explanation:

Humans have 23 chromosome pairs, out of which one pair determines the biological sex of an individual. Females have two X chromosomes, while males have one X and one Y chromosome. Mothers always pass on an X chromosome to their children, while fathers can pass on an X or Y chromosome. The sex of the child will depend on which chromosome it inherits from its father.

In guinea pigs, black fur color is dominant and brown fur color is recessive. Several matings between the same male black guinea pig and female brown guinea pig produce a total

of 30 black guinea pigs and no brown guinea pigs. What are the genotypes of the parents?





A. Bb x Bb

B. BB x bb

O C. Bb x bb

D. BB x BB

Answers

Answer:

BB x BB

Explanation:

All of the offspring inherit traits from the dominant gene. This means there are no recessive present.

Cephalization is (a) the division of the trunk into a scolex, neck, and body. (b) the presence of a head with sense organs. (c) sexual reproduction involving self-fertilization. (d) the ability to replace lost body parts. 2. Flatworms (a) are radially symmetrical. (b) possess a body cavity. (c) reproduce by parthenogenesis. (d) have tissues derived

Answers

Answer:

1. a

2. d

Explanation:

Thats the answer

what organ Works to keep your blood plasma isotonic ​

Answers

The kidneys.

The kidneys keep blood isotonic by removing excess salt and water through urine.

In prescriptive science investigations, scientists often make observations to understand the interacting parts of a complex____ A.Control B.Hypothesis C.procedure D. System

Answers

Answer:

The correct answer is D.

Explanation:

Descriptive science investigation is a study model, according to which a phenomenon is approached and its elements are characterized and its conceptualized, according to a series of methods used for data collection and expression of the same, that is, through this type of research, which uses the method of analysis, it is possible to characterize an object of study or a specific complex system, point out its characteristics and properties. The main objective of this research is the relationship between the factors, the actors and the variables. Researchers not only collect data through  observation but are in charge of studying and analyzing to obtain meaningful results that can contribute to knowledge.

T/F After cell division, each new cell will contain the same genetic information as
the original cell
A. True
B. False

Answers

Answer:

True

Explanation:

in cell division asextually new cells Contain same genetic information

If you are using a microscope with an ocular lens of 10x and an objective lens of 4x, what is the total magnification of the specimen you are viewing.

Answers

Answer:

The total magnification is 40x.

Explanation:

In order to calculate the total magnification of a microscope, we have to multiply the magnification of the used lenses. In other words, for this case:

Total Magnification = Ocular lens' magnification * objective lens' magnification

Total Magnification = 10 * 4 = 40

15 points, please help!!!
How many condons are shown on the mRNA strand below?

Answers

None. Please provide a picture

Which of the following characteristics is common to both acids and bases

Answers

Answer:

acids can burn people and baces can not and i am truley sorry if i am not right

Explanation:

What is the standard formula developed by the American society of anesthesiologists for payment of anesthesia services?

Answers

Answer:

The formulas for determining payment for surgical procedures requiring anesthesia are as follows: Anesthesia performed personally by the anesthesiologist (AA) Base units plus time units times conversion factor = X - 20% = fee

Answer:

X - 20% = fee.

Explanation:

Hi please help !!! Thankyou !!

Answers

Answer:

Both parents with the blood type A could produce offspring with blood types A or O

A scientific model can be used to _______.
a.
replace study of the actual system
b.
better understand systems and processes
c.
complicate ideas
d.
all of the above


Please select the best answer from the choices provided

A
B
C
D

Answers

Answer:

B

Explanation:

Scientific models are used to gain a better understanding of systems and processes. 

B hope that helped lmk if it’s right ight

The Venn diagram compares organic and inorganic compounds.



Which statement belongs in the space marked X?

Made from phosphorus and oxygen
Not made from living things
Made from every element except carbon
Not made from elements

Answers

Answer: b, not made from living things

Explanation: took the test

Answer:

b

got it right

Why are stop and start codons signals necessary for protein synthesis

Answers

Answer:

The start and stop codons —part of the mRNA's genetic code— are necessary because they indicate the exact length a protein should have.

Explanation:

The sequence of bases contained in an mRNA strand is the genetic code that allows the synthesis of specific proteins.  The mRNA information is organized into triplets or codons, whose function is :

To code the amino acids that will be incorporated into the protein to be synthesized. To establish the sequence of amino acids of a peptide or protein, which depends on the sequence of codons formed by nitrogenous bases. To mark the beginning of the synthesis, with the starting codon that, in addition, codes the amino acid Methionine. Establish the end of the synthesis, through the three stop codons, UAA, UAG and UGA.

The importance of the start and stop codons is that they establish the exact length that, genetically established, a specific protein must have.

chemical composition of the Rough endoplasmic reticulum

Answers

Answer:

The rough endoplasmic reticulum consists of two dimensional flattened sacs formed from phospholipids and ribosomes.

Explanation:

Genes within DNA are expressed through transcription and translation of the genetic code.

The rough Er contains important machinery in gene expressions, Ribosomes embedded in a lipid membrane. These are small sub cellular organelles that carry out a specific function of protein manufacturing and modulation. They contain the translocon complex, integral to the translation process

The endoplasmic retuculum consists of folded phospholipid membrane sheets or cisternae- these are mainly two dimentional, and form flat sacs.These provide small specific environments from the cytosol in the cytoplasm- they span the entire cytoplasm.

Making decisions require: no thought a little thought a lot of thought varying amounts of thought

Answers

Answer:

Varying amounts of thought

Explanation:

Some decisions, like what you should eat for lunch, require very little thought but others require a lot of thought, like choosing a college.

Answer:

varying amounts of thought

Explanation:

When ants get food from plants and protect the plants from being eaten, what kind of relationship do these two species have? Plzzzz help!!!

a) mutualistic

b) parasitic

c) commensalistic

d) harmful

Answers

Answer:

Myrmecophytes, otherwise known as ant-plants, share a mutualistic relationship with a colony of ants. This is when two organisms of different species each benefit from the activity of the other. There are over 100 different genera of myrmecophytes that can be found across the world's tropical regions

Explanation:

g Catalyzes a substrate level phosphorylation - breaks down sucrose to glucose and fructose - is a type of isomerase - removes one glucose residue from glycogen a. phosphorylase b. sucrase c. phosphohexose isomerase (also called glucose-6-phosphate isomerase) d. pyruvate kinas

Answers

Answer:

A phosphorylase removes one glucose residue from glycogen.

Sucrase breaks down sucrose to glucose and fructose.

Phosphohexose isomerase is a type of isomerase.

Pyruvate kinase catalyzes substrate-level phosphorylation.

Explanation:

A phosphorylase removes one glucose residue from glycogen.

The breakdown of glycogen requires a glycogen phosphorylase. It removes a glucose residue from glycogen in the form of glucose-1-phosphate.

Sucrase breaks down sucrose to glucose and fructose.

To metabolize a sugar different from glucose, an organism first has to transform it into glucose so it can then enter the glycolysis. Sucrose is a disaccharide formed by glucose and fructose bonded by an α-1,2 bond. Sucrase breaks down sucrose to glucose and fructose.

Phosphohexose isomerase is a type of isomerase.

It transforms glucose-6-phosphate into fructose-6-phosphate. This is the second step in glycolysis or the Embden-Meyerhof-Parnas pathway.

Pyruvate kinase catalyzes substrate-level phosphorylation.

It transforms phosphoenolpyruvate to pyruvate, releasing in the process an ATP molecule. This is the final step in the Embden-Meyerhof-Parnas pathway.

Which of the following is NOT an advantage to using a hydroponic system?
Lower water and nutrient costs
Higher crop yields
Diseases spread slowly through the system
O Faster plant growth

Answers

Diseases spread slowly through the system

Explanation:

It's the only disadvantaged one

Which of the following best explains how the phospholipid bilayer of a transport vesicle contributes to cellular functions?
A
The phospholipid bilayer allows the vesicle to fuse with the Golgi apparatus and the plasma membrane, allowing the exocytosis of proteins.
B
The phospholipid bilayer physically connects the nuclear envelope to the rough endoplasmic reticulum, thus increasing the rate of transcription and translation.
С
The phospholipid bilayer of a transport vesicle contains chemicals that digest the proteins made in the rough endoplasmic reticulum.
D
The phospholipid bilayer contains enzymes that catalyze the conversion of hydrogen peroxide to water and oxygen.

Answers

Answer:A

Explanation:

The statement 'the phospholipid bilayer allows the vesicle to fuse with the Golgi and plasma membrane, allowing exocytosis' explains how the phospholipid bilayer of vesicles contributes to cellular functions (Option A).

The endomembrane system is an interconnected network of phospholipid-based membranes which includes the Golgi and plasma membrane.

This system (endomembrane system) is used by cells to transport substances to the extracellular medium.

Transport vesicles are vesicles that help to move proteins and other biomolecules from the interior of the cell to the extracellular medium.

The process by which transport vesicles fuse with the cell membrane to release their components to the extracellular environment is called exocytosis.

In conclusion, the statement 'the phospholipid bilayer allows the vesicle to fuse with the Golgi and plasma membrane, allowing exocytosis' explains how the phospholipid bilayer of vesicles contributes to cellular functions.

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Some gold mines are more than 10 miles deep.

True or False?

Answers

Answer:

true true true true true true true

How many molecules are in 0.585 mole of lactic acid?

Answers

Answer:

There are 3.523x10²³ molecules in 0.585 moles of lactic acid.

Explanation:

To solve this problem we need to use Avogadro's number, which is defined as the number of molecules (or atoms, in the case of an element) that are in 1 mole:

There are 6.023x10²³ molecules in 1.0 mole

With that information in mind, we convert lactic acid moles into molecules:

0.585 mol * 6.023x10²³ molecules/mol = 3.523x10²³ molecules

When a piece of liver is dropped into hydrogen peroxide, the peroxide bubbles vigorously as the hydrogen peroxide (H2O2) decomposes into water (H2O) and oxygen (O2) This range change in reaction rate suggests

that liver is a substrate for hydrogen peroxide

that liver contains lots of germs

the liver contains an enzyme that breaks down hydrogen peroxide

that hydrogen peroxide is a powerful enzyme

Answers

Answer: C

Explanation:

Catalyst is one enzyme from liver that breaks down harmful hydrogen peroxide into oxygen gas and water

When a piece of liver is dropped into hydrogen peroxide, the peroxide bubbles vigorously as the hydrogen peroxide decomposes into water and oxygen. This suggests the liver contains an enzyme that breaks down hydrogen peroxide. Thus, the correct option is C.

What is Catalase?

Catalase is a common enzyme which is found in nearly all living organisms which are exposed to oxygen gas. Catalase is a key enzyme which uses hydrogen peroxide, a nonradical Reactive Oxygen Species, as its substrate. Catalase enzyme is responsible for the neutralization through decomposition of hydrogen peroxide, thereby maintaining an optimum level of the molecule in the cell system which is also essential for the cellular signaling processes.

Catalase is an enzyme which causes or speeds up a reaction without being affected during the reaction. The enzyme catalase speeds up the breakdown of hydrogen peroxide into water and oxygen gas.

Therefore, the correct option is C.

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