g A team of researchers suspects that RegX may play a role in regulating cytoskeletal reorganization by activating KinY. To test whether these two proteins can interact, they conducted two variations of the following experiment: 1) Transform mouse cells with expression plasmids encoding the proteins of interest. 2) Express proteins of interest inside cells. 3) Lyse cells 4) Conduct a Co-IP on the cell lysate against GST or GST-tagged KinY (depending on the specific experiment). 5) Run western blots to see which proteins are present in the immunoprecipitates. The results _________________________________________.

Answers

Answer 1

Answer:

It is expected to observe the immunoprecipitation of the GST-tagged KinY complex, thereby indicating the role of RegX in the mechanism of cytoskeletal reorganization.

Expanation:

Western blot is a technique widely used in molecular biology laboratories in order to identify proteins and also to quantify their expression levels. This technique is useful to detect proteins with high specificity, in this case the technique has been used for the detection of an immunoprecipitated (i.e., GST-tagged KinY complex) in order to confirm the role of the upstream protein RegX in cytoskeletal organization.


Related Questions

What kinds of waves do earthquakes produce?

• seismic waves
• longitudinal waves
• transverse waves
• heat waves

Answers

Answer: Earthquakes produces seismic waves.

Answer:seismic waves

Explanation:

In dogs, wire hair is due to a dominant gene (W), smooth hair id due to its recessive allele.a. If a homozygous wire-haired dog is mated with a smooth-haired dog, what type of offspring could be produced?b. What types of offspring could be produced in F2?c. Two haired dogs are mated. Among the offspring of their first litter is a smooth-haired pup. If these two wire-haired dogs mate again, what are the chances that they will produce another smooth-haired pup? What are the chances that the pup will be wire-haired?d. A wire-haired male is mated with a smooth-haired female. The mother of wire-haired male was smooth-haired. What are the phenotypes and genotypes of the pups they could produce?

Answers

Answer:

a) All the offspring produced would be a heterozygous in the first generation (F1 generation) with genotype: Ww, Ww, Ww, and Ww. Since wire hair is dominant, all would be wired haired.

b) In the F2 (second generation), we breed the heterozygous offspring from the F1 generation together (Ww x Ww). This will produce 3:1 ratio of dominant:recessive phenotypes having 3/4 of the offspring wire-haired (1/4 WW homozygotes and 1/2 Ww heterozygotes) and 1/4 will be smooth-haired (ww). Also the genotype would have the ratio 1:2:1 (i.e 1 homozygote WW, 2 heterozygote Ww and 1 smooth hair)

c) the chances of producing a smooth-haired pup is 1/4, and the chances of producing a wire-haired pup are 3/4.

d) Therefore, 1/2 of the offspring will have the genotype Ww and be wire-haired, and 1/2 of the offspring will be ww and be smooth-haired.  Also the phenotype ratio is 1:1 (1/2 is heterozygote wired hair and 1/2 is smoth haired)

Explanation:

Since the wire hair is the dominant gene (W) and smooth hair (w) is the recessive allele

a)  If a homozygous wire-haired dog is mated with a smooth-haired dog, All the offspring produced would be a heterozygous in the first generation (F1 generation) with genotype: Ww, Ww, Ww, and Ww. Since wire hair is dominant, all would be wired haired.

b) In the F2 (second generation), we breed the heterozygous offspring from the F1 generation together (Ww x Ww). This will produce 3:1 ratio of dominant:recessive phenotypes having 3/4 of the offspring wire-haired (1/4 WW homozygotes and 1/2 Ww heterozygotes) and 1/4 will be smooth-haired (ww). Also the genotype would have the ratio 1:2:1 (i.e 1 homozygote WW, 2 heterozygote Ww and 1 smooth hair)

c) If two wire-haired dogs produce a smooth-haired pup, that means that both parents must be heterozygotes (Ww) having a pair of dominant W allele and recessive w allele to pass on to the offspring. Therefore, if these two dogs were to mate again (Ww x Ww), the chances of producing a smooth-haired pup is 1/4, and the chances of producing a wire-haired pup are 3/4.

d) If the mother of the wire-haired male was smooth-haired, that means that the recessive allele w had been passed on to the male making the male a   heterozygote (Ww). When this male mates with a smooth-haired female (ww), the cross is Ww x ww. Therefore, 1/2 of the offspring will have the genotype Ww and be wire-haired, and 1/2 of the offspring will be ww and be smooth-haired.  Also the phenotype ratio is 1:1 (1/2 is heterozygote wired hair and 1/2 is smoth haired)

help meeeeeeeeeeeeeeee

Answers

Answer: b

Explanation:

Answer:

B

Explanation: I took the test :)

What is the diference between the axial and appendicular skeleton

Answers

Answer:

The axial skeleton makes up our central axis and consists of the following bones: skull, vertebrae, ribs and sternum. The appendicular skeleton consists of the limbs and girdles.

please mark brainliest

Explanation:

what is genetic code​

Answers

Answer:

The genetic code is the set of rules by which information encoded in genetic material (DNA or RNA sequences) is translated into proteins (amino acid sequences) by living cells.

Explanation:

Answer:

The genetic code is the set of rules used by living cells to translate information encoded within genetic material into proteins.

is essential to the body's ability to use iron.
A
Fluoride
B
Phosphorous
C
Chromium
D
Copper

Answers

We're no strangers to love

You know the rules and so do I

A full commitment's what I'm thinking of

You wouldn't get this from any other guy

I just wanna tell you how I'm feeling

Gotta make you understand

Never gonna give you up

Never gonna let you down

Never gonna run around and desert you

Never gonna make you cry

Never gonna say goodbye

Never gonna tell a lie and hurt you

We've known each other for so long

Your heart's been aching but you're too shy to say it

Inside we both know what's been going on

We know the game and we're gonna play it

And if you ask me how I'm feeling

Don't tell me you're too blind to see

Never gonna give you up

Never gonna let you down

Never gonna run around and desert you

Never gonna make you cry

Never gonna say goodbye

Never gonna tell a lie and hurt you

Never gonna give you up

Never gonna let you down

Never gonna run around and desert you

Never gonna make you cry

Never gonna say goodbye

Never gonna tell a lie and hurt you

Never gonna give, never gonna give

(Give you up)

(Ooh) Never gonna give, never gonna give

(Give you up)

We've known each other for so long

Your heart's been aching but you're too shy to say it

Inside we both know what's been going on

We know the game and we're gonna play it

I just wanna tell you how I'm feeling

Gotta make you understand

Never gonna give you up

Never gonna let you down

Never gonna run around and desert you

Never gonna make you cry

Never gonna say goodbye

Never gonna tell a lie and hurt you

Never gonna give you up

Never gonna let you down

Never gonna run around and desert you

Never gonna make you cry

Never gonna say goodbye

Never gonna tell a lie and hurt you

Never gonna give you up

Never gonna let you down

Never gonna run around and desert you

Never gonna make you cry

Several proposals have been made to increase the use of renewable energy. Which of these will help most toward that goal?

Question 42 options:

Design cleaner burning coal fired power plants.


Reduce the use of plastics in the waste stream.


Increase the use of solar electricity generation.


Design cleaner methods for fracking for natural gas.

Answers

Answer:

Increase the use of solar electricity generation.

Explanation:

The correct option would be to increase the use of solar electricity generation.

Solar electricity is a form of electricity generated by capturing the energy from the sun using special device known as solar panel. The captured energy is stored in a chemical cell which is then used to generate electricity. Because the will always exist and unending, the form of energy derived from it is referred to as renewable.

Hence, increasing the use of solar electricity generation will lead to a corresponding increase in the use of renewable energy.

Coal burning and natural gas are non-renewable energy sources while reducing the use of plastics in waste streams have no bearing on increasing the use of renewable energy.

Which of the folowing groups of bacteria a predominant in all dental plaques

Answers

Answer:

Streptococcus

Explanation:

Two types—

Streptococcus sanguis and Streptococcus mutans

Which state of matter does NOT take the shape of its container? Solids Liquids Gases please help ASAP

Answers

Answer: Liquid

Explanation:

Liquid because, liquids have a definite volume. However, because the particles can move about each other rather freely, a liquid has no definite shape and takes a shape dictated by its container.

A protein's primary structure dictates its final chemical behavior.
TRUE
FALSE

Answers

Answer:

true

Explanation:

The string of amino acids that makes up a protein's primary structure later determine the protein's final chemical behavior

Ture
Because it just is


1. In a nuclear power plant, where does nuclear fission occur?

Answers

Answer:

nuclear fission reaction takes place in nuclear reactor

Explanation:

Answer:

Nuclear reactor

Explanation:

When an atom of nuclear fuel (uranium) absorbs a neutron, the uranium will fission into two smaller atoms (waste) and release one to three neutrons. The kinetic energy of the waste is used to heat the water for the steam turbine. The neutrons are used to fission the next lot of uranium atoms and the process continues.

pls mark brainliest

What is the freezing point in degrees Fahrenheit? What is it in Celsius?

Answers

Answer:

Freezing point:

  Fahrenheit: 32°      

celsius: 0°

Explanation:

Help please ill give you brainliest !!!!

Answers

Answer: I am not totally sure but I would say the answer would be (D)

Explanation: Exposure to Diborane will not kill you or anything like that but it will most likely cause you to have a cough, shortness of breath (but not to bad), and eye/skin irritation.

exposure to diborane gas causes chest tightness, coughing, skin, eye & nose irritation so I would say C. You can’t die from it but the more serious the exposure the more serious the symptoms and you can be hospitalized.

Which statements describe attributes of an artisan well? Select two options

Answers

Answer:

B. uses natural pressure

E. has groundwater stuck between impermeable layers

Explanation:

Artesian well has the same attributes of having the presence of water like that of a regular well. The major difference is that the artesian well has water which comes out to the surface without the application of pressure. This is due to the presence of a positive pressure within the underlying rocks and its environs which forces the water out to the surface.

It has natural pressure and doesn’t require a pump and it also has groundwater stuck between impermeable layers.

Among rabbits, short hair is dominant over long hair. What would be the appearance of the
rabbits if a heterozygous short hair female were mated with a homozygous short hair male?

Answers

Answer: 100% short hair

Explanation: there will 50% homozygous short hair (HH) and 50% heterozygous short hair (Hh).

Check out the punnet square down below to see how it’s done.

Which is the correct order of the states of MITOSIS?

Answers

C it’s going to be the 3rd option

Define Liquid Crystal Display (LCD) ?​

Answers

Answer:

flat-panel display or other electronically modulated optical device that uses the light-modulating properties of liquid crystals combined with polarizers. Liquid crystals do not emit light directly, instead using a backlight or reflector to produce images in color or monochrome.

Explanation:

Which traits are NOT inherited? (Check ALL correct answers.)

a) A boy has curly hair
b) A woman has a suntan
c) A girl has pierced ears
d) A dog has yellow fur
e) A cat has its claws removed
f) A girl has attached earlobes
g) A man has green eyes
h) A boy has long hair

Answers

Answer:

Listed

Explanation: B, C, E are your answers

A scientist testing the effects of a chemical on apple yield sprays an orchard with the chemical. A second orchard does not receive the chemical. In the fall, the number of apples harvested from each orchard is counted. Which of the following variables must be controlled in this experiment?
1. the amount of water that each orchard receives
2. the species of trees in the orchard
3. the number of apples that are produced by each tree
4. the size of each of the orchards

Answers

Answer:

1 and 2

Explanation:

1) The exercise scientist is looking for the effects of a chemical between an apple crop to which it is administered and another to which it is not, 4 options are presented, of which it is essential to count as a variable the amount of water each Orchard and tree species in the orchard, since they can generate alterations in the results, the other two variables of the exercise such as number of apples and size of the orchards are not significant and their variations do not affect the scientist's objective.

The fusion of a _______________ and a _________________ produces a zygote with 46 (2n) chromosome number.

Answers

Answer:

The fusion of a egg and a sperm produces a zygote with 46 (2n) chromosome number.

Fish maintain water flow over their gills by swimming rapidly with their
mouths open or by taking in water through their mouths.
TRUE
FALSE

Answers

Answer: this is false fish only have water flow through there gills not mouths and thay dont have to be swimming

Which of the following
options does not
describe any kind of
stem cell?
A cell that can only
give rise to one cell
type
A cell that can give
rise to many different
cell types
A cell that can give
rise to all cell types

Answers

Answer:

the first one.

I on accidentally erased the explanation thing. but stem cells such as pluripotent cells can rise to all cell types which means that since the first one is saying that stem cells can only give rise to one cell type is incorrect.

Your blood type is O. is this a gen or a gean

Answers

Blood type O is gene recessive

What is 5% of 90 cents?

Answers

Answer:

4.5

Explanation:

:0

The answer is 4.5 us cents

Number of
Protons
Number of
Neutrons
Atomic
Number
Mass
Number
Element
(symbol)
4
2
А
B
C
D
12
10
Using the periodic table complete the table to describe each atom Type in your answers

Answers

Answer:4 2

Explanation:

How do the circulatory and respiratory systems play a role in track and field?

Answers

Answer:

Track and field requires a lot of running

Explanation:

When someone runs, blood needs to be circulated throughout the whole body.  Constant blood is pushed throughout the body due to blood vessels in our circulatory system.  The heart pumps faster so more blood is produced. Oxygen is needs to be reached to muscles.  When someone runs, they tend to breathe heavier.  Heavy breathing and more breathing is done by the respiratory system.  Lungs need to filter air and therefore the respiratory system works hard as well

The respiratory system works directly with the circulatory system to provide oxygen to the body. Oxygen taken in from the respiratory system moves into blood vessels that then circulate oxygen-rich blood to tissues and cells.

PLEASE HELP ME IM CONFUSED

Answers

The sea because if you ever been to the beach when the waves pick up it becomes hard

Answer:

B In space

because light moves faster where there is no gravity

What type of pollutants are emitted directly from identifiable sources?

Answers

Answer:

Point-source pollutants.

Explanation:

Point-source pollutants are emitted from single identifiable sources. For instance, untreated waste water from a particular oil refinery or factory is a point-source pollutant.

In birds, having long feathers (L) is dominant to short feathers (l). A homozygous dominant bird and homozygous recessive bird mate. After creating a punnett square, give the genotypes and phenotypes of the four resulting offspring (include the number of each)

Answers

Answer:

All the genotypes would be heterozygous (L l) and the phenotypes would be long feathers

Explanation:

help meeeeeeeeee plzzzzzzzzzzzzz

Answers

Answer:

it's d

Explanation:

Answer:

D

Explanation:

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