when determining the number of virus particles in a suspension, why is the number obtained always less than the actual viral titer?

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Answer 1

Because not all viral particles are 100% fective at spreading infection. In order to count intact virus particles in a sample, a virus counter uses fluorescence to find colocalized proteins and nucleic acids.

Samples are evaluated as they pass under a laser beam after being dyed with two dyes, one particular for proteins and one specific for nucleic acids. To gauge a virus' potency against host cells, an infectious viral titer assay is performed. Plaque assays, which include counting distinct plaques (infectious units and cellular dead zones) in cell culture, continue to be among the most precise techniques for the direct measurement of infectious virions and antiviral compounds.

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Related Questions

the highlighted structure is an important clinical marker. what is its significance? matches up with the aorta

Answers

Its significance coincides with the aorta and lines up with the upper boundary of the heart.

What structure is created when the zygomatic bone's temporal process and the highlighted structure are combined?

The zygomatic process of the temporal bone and the temporal process of the zygomatic bone work together to generate the zygomatic arch. The temporal fossa is the shallow area above the zygomatic arch. The infratemporal fossa is the region deep to the posterior mandible and inferior to the zygomatic arch.

Which structure's bones are highlighted?

The sternum is the bone that has been emphasized. The sternum, which is situated in the middle of the chest, is where the ribs are attached. The breast bone is another name for the sternum.

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which type of protein is responsible for transporting sodium and potassium ions across the plasma membranes?

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Answer:

The Sodium-Potassium Pump

In active transport, as carrier proteins are used t o move mater ials against their concentration gradient, these proteins are k nown as pumps

Explanation:

Hopefully this is the answer you are looking for.

Where would you find the genotypes for the offspring or babies?

Answers

The genotypes of the kid are created at the junction of the parent's alleles. The Punnett square findings may be used to calculate the odds of certain genotypes and phenotypes.

Punnett squares are diagrams used to forecast the outcomes of traditional breeding experiments. They believe in Mendelian inheritance, as well as the rules of segregation and independent assortment. During meiosis, chromatids are divided so that each gamete receives just one allele.

Furthermore, various genes are distributed and inherited independently of one another; the chance of one phenotype does not alter the probability of another. The genotypes of both parents must be known to generate a Punnett square.

The alleles of one parent are displayed across the top of the table, while the alleles of the other parent are listed along the left side. The genotypes of the child are created at the junction of the parent's alleles. The Punnett square findings may be used to calculate the odds of certain genotypes and phenotypes.

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what types of mutant commonly result from regulatory mutations that increase the rate of transcription of a gene? see section 4.1 (page 107

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The types of mutant commonly result from regulatory mutations that increase the rate of transcription of a gene is hypermorphic.

Generally speaking, increasing the rate of transcription causes the generation of extra gene product, which causes extra gene activity.

A mutant could produce an active product with a new and different function or more of the active product (hypermorph) if it has a gain-of-function allele (neo morphs). Last but not least, antimorph alleles, sometimes referred to as dominant negative mutations, have an activity that is dominant and opposing to that of the wild-type product.

As a result, mutant alleles can arise from mutations (changes in a gene's sequence) that do not produce the same quantity or kind of active product as the wild-type allele. One of five categories—amorph, hypomorphic, hypermorph, neomorph, and antimorph—can be applied to any mutant allele.

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6. Which statement BEST describes natural selection?

population growth of a species in advantageous environments

advantageous mutations that are selected by nature

population growth of a species found in nature.

advantageous growth due to an organism's environment

Answers

Answer:Advantageous mutations that are selected by nature

Explanation: Natural Selection is the process in which certain traits that are created by mutations are able to allow the organism to thrive. Those with these mutations are likely to live better and spread that trait to future generations.

B. advantageous mutations that are selected by nature. For inference, the definitive of the word natural selection.

how does one determine the minimum inhibitory concentration in the broth test? select one: a. reading the concentration on the plastic strip b. testing the fic index c. looking at the growth inhibition zone d. examining the broth for lack of turbidity

Answers

One can determine the minimum inhibitory concentration in the broth test by examining the broth for lack of turbidity.

The correct option is option d.

The minimum inhibitory concentration happens to define the in vitro levels of susceptibility or the resistance of some particular bacterial strains to the applied antibiotic. Reliable assessment of the minimum inhibitory concentration has a very significant impact on the therapeutic strategy which will be chosen and also affects efficiency of an infection therapy.

The minimum inhibitory concentration or the MIC is basically the lowest concentration of a chemical, which is usually a drug which happens to prevent the visible growth of the bacterium. The determination of the minimum inhibitory concentration is basically based on turbidity. High turbidity would mean high concentrations of anti microbial drugs whereas lower the turbidity, the lower will be the microbial concentration.

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ou generate a plant with a mutation in the enzyme rubisco, and this mutant enzyme now shows optimal activity at a ph of 7.5. what consequences might this mutation have on the regulation of the calvin cycle?

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The mutation in rubisco leading to optimal activity at a pH of 7.5 may affect the regulation of the Calvin cycle by altering the rate of carbon dioxide (CO2) fixation.

If the pH deviates from 7.5, the activity of the mutated rubisco enzyme may decrease, leading to a decrease in the rate of CO2 fixation. This can impact the rate of photosynthesis and the overall efficiency of the plant's energy production.

Additionally, changes in pH can also affect the activity of other enzymes involved in the Calvin cycle, potentially disrupting the entire process and leading to growth and metabolic abnormalities in the plant.

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if organisms used only twelve different amino acids to build proteins instead of twenty, what would be the smallest number of dna or rna nucleotides needed to code for an amino acid?

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The minimum number of DNA or RNA nucleotides required to code for an amino acid is 2, which would be the case if organisms only employed twelve different amino acids to construct proteins rather than twenty.

Amino acids: what are they?

Amino acids are the building components of proteins. An extensive chain of amino acids makes up proteins. There are countless different types of proteins in your body, each of which has a vital function. Each protein has a unique arrangement of amino acids.

The protein can have many forms and perform a number of biological roles depending on the sequence. You can compare amino acids to the letters of the alphabet. You can create new words by combining letters in novel ways. Similar to how proteins are made, amino acids can be combined in many ways to create a variety of proteins.

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how does human anatomy make it difficult to record an electroencephalogram or eeg? (select all that apply)

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The Scalp, Skull, and layer of CSF that distance the recording electrodes from the surface of the brain results in a reduced reading of the waves.

EEG: What is it?

Using tiny metal discs (electrodes) affixed to the scalp, an electroencephalogram (EEG) is a test that gauges electrical activity in the brain. Electrical impulses are the primary means of communication for brain cells, which are constantly active—even while you're asleep. This activity appears as wavy lines on an EEG recording.

One of the key epilepsy diagnostic procedures is an EEG. Other types of brain conditions can also be identified with an EEG. An EEG can identify variations in brain activity that may help in the diagnosis of several brain illnesses, including epilepsy or another seizure disorder. An EEG could be useful for identifying or treating:

brain cancerhead injury-related brain damageVarious conditions can lead to brain dysfunction (encephalopathy)sleep problems

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Correct question:

how does human anatomy make it difficult to record an electroencephalogram or EEG? (select all that apply)

a) Scalp

b) Skull

c) Layer of CSF

A DNA molecule is made up of long chains of nucleotides. A DNA nucleotide consists of a

Answers

A DNA nucleotide consists of a pentose sugar, a phosphate group, and nitrogenous bases (purines and pyrimidines).

What is a DNA nucleotide?

Nucleotides are monomers of nucleic acids such as DNA and RNA. Each DNA nucleotide contains deoxyribose sugar (pentose sugar), a phosphate group, and nitrogenous bases (purines and pyrimidines).

Purine contains adenine and guanine, while pyrimidine contains cytosine and thymine.

A purine always makes a hydrogen bond with a pyrimidine. Adenine forms two hydrogen bonds with thymine and guanine forms three hydrogen bonds with cytosine.

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the endergonic conversion of adp to atp requires more than 30 kj/mol. the hydrolysis of phosphoenolpyruvate to pyruvate drives the conversion of adp to atp. what must be true about this hydrolysis?

Answers

The hydrolysis reaction is more exergonic than the endergonic conversion of ADP (the endergonic conversion of adp to atp requires more than 30 kj/mol) which is the truth about how phosphoenolpyruvate is converted to pyruvate to promote the conversion of adp to atp.

An ATP molecule becomes an ADP when the tail of the molecule's phosphate group is severed (via hydrolysis) (adenosine diphosphate). That hydrolysis produces energy and is an exergonic reaction. The phosphate is attached to ATP by weak bonds. Although they are not strong (if they were strong, it would take a lot of energy to break them), they are known as high energy bonds. Consider ATP as a spring-loaded molecule with the last phosphate simply tacked on.

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how does execution, discovery, and lateral movement (in this order) take place with spearfishing attachments?

Answers

Spearfishing attachments are used for dynamic data exchange, network service inspection, and login scripts.

What technological advancement uses ruse mechanisms to fool hackers?

In order to entice online attackers and to detect, block, and research attempts to hack into information systems without authorisation, a network-attached system called a "honeypot" is set up.

What TCP header field is in charge of identifying the receiving process?

The transmission and receiving processes are identified by TCP using the IP address and port number (16-bit value), respectively. A TCP connection is uniquely identified by the two end sockets, which are pairs of an IP address and port number.

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Explain the role of the mitochondria in the metabolism of eukaryotic cells.

Answers

Mitochondria Play a critical role And generation of metabolic energy in eukaryotic cells

raits thought to be caused by the combined effects of multiple genes and environmental factors are referred to by which term?

Answers

Complex traits are used to describe traits that are thought to be caused by the combined effects of multiple genes and environmental factors. Here option D is the correct answer.

Complex traits are traits that are influenced by multiple genes and environmental factors. These traits are often referred to as "polygenic" or "multifactorial" traits, as they result from the interplay between many different genetic and environmental factors. Examples of complex traits include height, body mass index, intelligence, and various diseases such as diabetes, heart disease, and cancer.

In contrast, simple traits are traits that are influenced by a single gene and have a straightforward pattern of inheritance. These traits are often referred to as "Mendelian" traits, after Gregor Mendel, the father of genetics. Examples of simple traits include eye color, blood type, and sickle cell anemia.

Complete question:

What term is used to describe traits that are thought to be caused by the combined effects of multiple genes and environmental factors?

A. Simple traits

B. Polygenic traits

C. Mendelian traits

D. Complex traits

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I’m confused on how to exactly draw what it’s asking. Please help with the enzymes.

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Proteins called enzymes are made up of amino acids connected by one or more polypeptide chains.

What are Enzymes?

The fundamental structure of a polypeptide chain refers to this arrangement of amino acids.

This in turn dictates the enzyme's three-dimensional structure, including the active site's shape. The localized polypeptide chain structures, such as -helices or -sheets, are referred to as the secondary structure of a protein.

The tertiary structure of a polypeptide chain refers to the entire three-dimensional fold into a protein subunit. One or more subunits (monomers) can make up a protein (e.g., a dimer). Its quaternary structure refers to the subunits arranged in three dimensions.

Therefore, Proteins called enzymes are made up of amino acids connected by one or more polypeptide chains.

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What is an example of parental care?

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When the birds and animals like those belonging to group of mammals keep their children closely attached to them when they are young, it serves as an example of parental care.

Parental care is the act of protecting and raising the young ones until they are able to survive on their own. The mode parental care may change from one person to another. The majority of parental care can be observed in mammals and birds.

Mammals are the vertebrate animals that are characterized by the presence of mammary glands in females. These are the most advanced type of animals with complete organ system level of organization. The examples are: humans, monkeys, kangaroos, etc.

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ANYONE PLEASE HELPPP
DUE TODAY :///

Answers

Answer:

correct answer is option c

Do hiberations and camouflage
have low or high precipitation

Answers

Hibernation and camouflage are not directly related to precipitation levels, as hibernation is a type of sleep that occurs during the winter months and camouflage is a method of concealment used by animals. However, the amount of precipitation in an area is largely determined by its climate.

What is hibernation?

Hibernation is a condition of hibernation that certain animals adopt during the winter or cold weather to save energy. Hibernation causes an animal's heart rate, breathing rate, and body temperature to drop dramatically, allowing it to subsist on stored energy until conditions improve.

In general, hibernation and camouflage have no direct association with precipitation amounts. Hibernation is a sort of slumber that certain animals experience throughout the winter, while camouflage is a way of hiding employed by animals to fit in with their surroundings. In general, the amount of precipitation in a region is governed by its climate, which is mainly independent of hibernation and concealment.

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What best describes the radiation emitted from the sun?
A. It travels at different speeds
B. It is made up of one type of electromagnetic wave
C. It is made up only of yellow light
D. It is made up of different frequencies of light

Answers

Answer: B. Radiation emitted from the sun contains one type of electromagnetic wave .

meiosis in animals is responsible for what

Answers

Answer: formation of gametes cells I think

Explanation:

explain where the carbon dioxide comes from​

Answers

Answer:

Carbon dioxide comes from a variety of sources, both natural and man-made. On the natural side, carbon dioxide is released through respiration, decay of organic matter, volcanic eruptions, and the ocean. On the man-made side, carbon dioxide is released through the burning of fossil fuels, deforestation, and industrial processes.

Answer:

Hi there. Carbon dioxide comes from plants when they go through photosynthesis and take oxygen from the air and convert it to carbon dioxide!

Brainiest? Can you click on the crown on this answer? Thanks!

Fats and oils are made up of ______________ and ______________.

Answers

Glysrol and fatty acids

the classification of an organism and the scientific name of an organism are different because___.

Answers

Explanation:

the full classification of organisms will include more categories of organisms than their scientific names. The scientific names of organisms include a single nomenclature, but the full classification includes various nomenclatures.

Explain why the Tonga Trench region and the Chile Trench region produce earthquakes with different focal depths. be specific

Answers

I have no idea to what this answer would be

What are three factors that could prevent an organism from long ago from ever turning up in a fossil collection today?

Answers

Answer:

Explanation:

The organism generally must have hard parts such as shell, bone, teeth, or wood tissue; the remains must escape destruction after death; and the remains must be buried rapidly to stop decomposition.

Which complex in the electron transport chain does not contribute to the proton gradient across the mitochondrial membrane?

Answers

The complex in the electron transport chain does not contribute to the proton gradient across the mitochondrial membrane is complex II

Electron transport is the final part of aerobic respiration which occurs in mitochondria to produce ATP. At this stage the NADH and FADH2 produced from previous processes such as glycolysis and the Kerb cycle are transferred to the inner mitochondrial membrane to be converted into ATP in the electron transport chain.

The electron transport chain involves three enzyme complexes embedded in the mitochondrial inner membrane that can transport enzymes and pump protons. Complex II is a component of electron transport that only accepts electrons from FADH2 and does not pump protons across the mitochondrial membrane. Therefore, this process produces a small amount of ATP.

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what is the most metabolically active organ in the human body? group of answer choices liver brain small intestine adrenal gland

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A) liver  is the most metabolically active organ in the human body.

In general ,liver is considered as the  most metabolically active ang greatly aerobic organ of the human body .Liver receives almost 29% of the blood in total that flow and  it use to extract almost 20% of the oxygen used by whole body. Liver is also answerable for the synthesis and degradation of carbohydrates, proteins, and lipids.

Liver use to converts the nutrients in our diets that can be used by our body, and also stores these substances and supply them to the cells  when needed. They also convert toxic substances  into harmless ones and help body to release them .

Hence, A is the correct option

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Determine the mean for each data group, and describe in a complete sentence how you calculated them.

Answers

The mean is the average of a data set. We can get it by making an addition of all the data set values, and then divide the result by the total number of values. In the example, the mean is 8.73.

Since I could not find the data groups, I will explain what the mean is and how to get it, and then I will provide an example for you to understand and use as a guide.

What is the mean?

The Mean, together with media and mode, is a central tendency measure. It is used in statistical analysis to identify the tendency of a data set concerning information inclination.

This measure is used to expose the result of a research in a resumed way to avoid providing extensive information.

The Mean is the average value of a data set.

To calculate the mean you just have to add all the data and divide it by the amount of data you have.

For instance, let assume our data set is as follows,

4.5,5,7,7,7.5,7.5,7.5,7.8,7.8,8,8,9,9,9.2,9.3,10,10,10.5,13,17

To get the mean we need to add these values and then, divide them by the total number of values.

Addition:

4.5 + 5 + 7 + 7 + 7.5 + 7.5 + 7.5 + 7.8 + 7.8 + 8 + 8 + 9 + 9 + 9.2 + 9.3 + 10 + 10 + 10.5 + 13 + 17 = 174.6

Total number of values: 20

Mean = (Σ values) / Total number of values = 174.6 / 20 = 8.73

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if a process kills 90% of the organisms per minute, how many minutes would it take to kill all organisms when starting with 100,000 organisms?

Answers

Option D: 6 minutes would be taken by the process to kill all organisms when starting with 100,000 organisms.

Sterilization is the process of physically or chemically eradicating or destroying the life of any type of germ in a medical setting. The primary sterilisers used in medical institutions include pressurized steam, dry heat, EtO gas, hydrogen peroxide gas plasma, and liquid chemicals. Sterilization is the act of expressing the truth. Unfortunately, some medical experts, technical, and business publications refer to sterilization and articles as "semi-sterile." If a process kills 90% of the organisms per minute, it will take 6 minutes to kill all organisms when starting with 100,000 organisms.

Chemical disinfectants are terms used to describe chemicals that are employed to eliminate all types of microbiological life. It's possible that the same disinfectant used for shorter exposure times will also be employed throughout the disinfection procedure (ie, higher levels of disinfection).

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Complete question is:

If a process kills 90% of the organisms per minute, how many minutes would it take to kill all organisms when starting with 100,000 organisms?

3 minutes

1 minute

2 minutes

6 minutes

During which of these processes is oxygen consumed?

Answers

The process of burning,
•When plants perform photosynthesis
• when aerobic bacteria breaks down dead algae in a pond
• when forests are burned to clear land for forming
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