What control showed that transformation and not conjugation or transduction was responsible for the results

Answers

Answer 1

When we talk of transformation,we are talking of a biological method of the alteration of gene in a living cell especially as seen in a bacterial cells.

As a result of the incoperation and uptake of foreign genetic material from it's surrounding, the said bacteria then takes up the exogenous DNA,this it does by binding it with specific receptors that are present in its cells membrane or it can do so through diffusion.

However,the foreign DNA will either get incorporated in the DNA of the host chromosome or it will act like a plasmid and them express itself in the cell of the new host.

Then in conjugation,we see the transfer of circular plasmid DNA,this is transferred from one bacterium to another and it is done through cell to cell contact.

Also, transduction is still another way of transferring genetic materials,here the DNA is transferred to another bacteria via bacteriophage through lysogeny.

Now,in a given experiment,to identify if the reaction is transformation and not configuration and transduction,a controls sample will be provided,this control sample will contain DNase coupled with the reaction mixture,if there is a revelation of an exogenous DNA by the test sample,and there is no expression by the control,then we know that is transformation,we know it is transformation because DNase cleave free DNA and it will not be able to cut the DNA that is present inside the cell of donor bacteria that is in conjugation or bacteriophage(transduction).

Therefore,the addition of DNase in control sample can determine if the transfer of genetic materials in the test samples is transformation and not transduction or conjugation.


Related Questions

(part 2)PLEASE HELP! IN A HURRY! WILL GIVE BRAINLIEST!

Answers

Answer:

i say 'a' lower cased

Explanation:

brainlest?

Which constellation is connected with lines in the picture?

Answers

where’s the picture

Answer:

big dipper

Explanation:

i took the test on k12

A food chain is the feeding relationships among all of the organisms in an ecosystem true or false?

Answers

Answer:

True

Explanation:

Hope its help ;-);-)

Answer:

FALSE

Explanation:

Identify the organelles in the cell. Label A Label B Label C Label D

Answers

Answer:

This question is incomplete

Explanation:

This question is incomplete but the completed/similar question with the diagram is in the attachment below.

First of all, the cell is a plant cell because of the presence of the large central vacuole present and also the green colour of the outer part of the cell (which is not a coincidence). Let's proceed to identifying the organelles.

Label A is the large central vacuole. This is not the nucleus as might be confused with because the nucleus is the next big reddish organelle to the right of the vacuole.

Label B is the cell wall. Because this is a plant cell, it has a cell wall which can be clearly seen to be the most outermost layer. While the next yellow boundary line is the plasma membrane.

Label C is the chloroplast. Recall from elementary biology that the chloroplast stores the green pigment chlorophyll. The green colour of this internal organelle makes it identifiable as the chloroplast.

Label D is the starch granule. The shiny semicrystalline structure of this organelle makes it identifiable as the starch granule.

Answer:

Chloroplast, Vacuole, Cell wall, Endoplasmic reticulum

Explanation:

Label A: Chloroplast

Label B: Vacuole

Label C: Cell wall

Label D: Endoplasmic reticulum

How do you think earthworms help the process of composting

Answers

Answer: Worm composting is using worms to recycle food scraps and other organic material into a valuable soil amendment called vermicompost.

Explanation: Worms eat food scraps, which become compost as they pass through the worm's body

Facts about physical changes in matter

Answers

Answer:

It is the change that are not easily reversible and new substances are formed. example burning of wood,rusting of iron, burning of coal, fermentation

Explanation:

pls hope it helps

Answer:

All physical changes are reversible and their mass does not change. Some physical changes examples are melting, freezing, and evaporating.

Explanation:

True or False about neutrons.
1. Discovered by Dimitry Mendeyev
2. Neutrons are the lightest of all subatomic particles
3. Neutrons subatomic particles with no charge
4. Neutrons are found inside the nucleus
5. Neutrons have six times the mass of a proton

Answers

Number 1 is true the second one is true the third is true number 4 is true and lastly number 5 is false I hope this helps you but I’m very confident!

Darius created what economy for the use of coins for goods ?


A. Trade

B. Barter

C.Capitalist

D. Money

Answers

Answer:

The correct answer is -  A. Trade

Explanation:

Darius the emperor of the Persian empire was the first Persian emperor to use a coin system for goods, services, and even for tax which makes his economy large and developed his empire, and created a new capaital. Darius used a gold minted coin system for trade for the goods and services and salaries to the army which made his empire popular and a large economy. His economy was a trade economy as coins were used for the different type of goods, foods and services.

What differentiates passive transport from active transport?
O Molecules move down a concentration gradient during passive transport.
Passive transport involves molecules moving across the cell membrane.
ATP is needed for passive transport to occur
Proteins have a role in passive transport.

Answers

The first option, molecules move down a concentration gradient during passive transport

Four movie tickets cost $48 . At this rate, what is the cost of 5 movie tickets

Answers

48/4 is 12 so 48+12 is 60. 5 tickets would be $60

11. An example of an atom that has no charge is one that has
A. 3 protons, 2 electrons, and 1 neutron.
B. 1 proton, 2 electrons, and 3 neutrons.
C. 2 protons, 2 electrons, and 1 neutron.
D. 3 protons, 1 electron, and 3 neutrons.

Answers

B. The neutrons cancel out the protons and electrons

Answer:

C. 2 protons, 2 electrons, and 1 neutron.

Explanation:

Which of the following statements is correct?


Farming is a relatively small industry that plays a small role in the economic success of the nation.

Farming is a relatively small industry that plays a large role in the economic success of the nation.

Farming is a relatively large industry that plays a large role in the economic success of the nation.

Farming is a relatively large industry that plays a small role in the economic success of the nation.

Answers

Answer:

c Farming is a relatively large industry that plays a large role in the economic success of the nation.

Sensory _____ are the parts of the body that change a stimulus into a nerve impulse and response. effectors receptors pathways potentials

Answers

Sensory receptors are the parts of the body that change a stimulus into a nerve impulse and response.

What is the difference between phenotype and genotype? A. Phenotype is the physical expression of a trait and genotype is all that alleles in the organism. B. Genotype is the physical expression of a trait and phenotype is all the alleles in the organism. C. Phenotype are the alleles that are mast in genotypes are the alleles that are expressed. D. Phenotype is when both allies are the same and genotype is when the alleles are different. ​

Answers

Answer:

A. Phenotype is the physical expression of a trait and genotype is all that alleles in the organism.

Explanation:

An organism's genotype is the combination of alleles found at a particular gene. The phenotype is how theses alleles are expressed to form a trait.

In a diploid organism there are two alleles for every gene. In simple Mendelian traits. one allele is dominant over the other, which is recessive. That means, that the phenotype displayed will be associated with the dominant allele whenever it is present.

Answer:

Explanation:

Phenotype -Physical

Genotype-Gene

What part of the bone produces blood cells?

Answers

Answer:

Red blood cells are formed in the red bone marrow of bones

Exposure the radiation such as an X-ray may change the sequence of
nitrogen bases in DNA. What is this specifically called?

1) a mutation
2) a translation
3 ) a replication
4) a transcription

Answers

Answer:

Mutation

Explanation:

What is the average for the following set of measurements?
27°C, 12°C, 31°C, 19°C, 23°C, 11°C, 17°C
O A. 20°C
O B. 140°C
O C. 28°C
O D. 7°C

Answers

A. 20°C once adding all numbers, the sum is 140. the amount of number is 7. once you divide the two ( 140/7 ) you get 20°

Why do Primary succession happen

Answers

Answer:

Explanation: Primary succession occurs when new land is formed or bare rock is exposed, providing a habitat that can be colonized for the first time. For example, primary succession may take place following the eruption of volcanoes, such as those on the Big Island of Hawaii. As lava flows into the ocean, new rock is formed.

This is the act of trying to find the answer to an unknown question by the use of experimentation and research.

Answers

Answer:

Question Answer

Inquiry This is the act of trying to find the answer to an unknown question by the use of experimentation and research.

Investigation This is the process of researching or studying a concept. Examination, inquiry

Explanation:

The main function of the circulatory system is to
O transport gases into and out of the body.
O transport nutrients, gases, and wastes.
O diffuse gases from the blood to lungs.
O filter water and wastes.

Answers

Answer:

Transport nutrients gas and waste. im pretty sure hope its right !

in the mitosis of onion root experiment, why does the onion root have to be crushed into smaller pieces​

Answers

It allows them to be flattened on a microscopic slide, so that the chromosomes of individual cells can be observed easily.

explain why did saturnino Fabros , a 20-year traffic enforcer veteran , was not a victim of cyber bullying? instead of Robert Blair carabuena who was in a video who slapped to saturnino
.​

Answers

Answer:Carabuena was caught on camera slapping MMDA traffic enforcer Saturnino Fabros after he was pulled over for a traffic violation. Image of page 12. Describe the acts of cyber bullying that the netizens did to Carabuena. ... such as "Patayin si Robert Blair Carabuena", "Like if you want Robert Blair Carabuena to be fired.

Explanation:

1. Melissa raises crickets at her pet store that she
sells for reptile food. She thinks that crickets
chirp more often when the temperature gets
warmer. She decides to conduct an experiment to
prove her theory
a. Manipulated variable
(IV)
b. Responding variable
(DV).
c. Controlled variable
d. Controlled variable
Hypothesis: If
then
because
_(manipulated variable)
(responding variable)

Answers

Answer:

a. Manipulated variable: TEMPERATURE

b. Responding variable: CRICKET CHIRP

c. Controlled variable: Same species of cricket

d. Hypothesis: IF the temperature is increased, THEN crickets will chirp more

Explanation:

Independent variable, also called the manipulated variable, is the variable that is changed or manipulated in an experiment. In this case, the independent variable is the TEMPERATURE.

Dependent or responding variable is the variable that responds to changes made to the independent variable. The dependent variable in this case is CHIRP OF THE CRICKETS.

Controlled variable is the variable that must be kept unchanged for all groups throughout the experiment. The controlled variable in this case is SAME SPECIES CRICKET USED.

Hypothesis is a testable explanation (via experimentation) to an observed problem or a given question. In this investigation involving Melissa, an hypothesis is: IF the temperature is increased, THEN crickets will chirp more.


Both animal and plant cells are eukaryotic cells. What is the difference between animal and plant cells?
OA
Plant cells have cell walls and chloroplasts, but animal cells do not
Animal cells have nuclei and mitochondria, but plant cells do not
OB
OC. Plant cells have nuclei and mitochondria, but animal cells do not
OD. Animal cells have cell walls and chloroplasts, but plant cells do not

Answers

Answer:

A. Plant cells have cell walls and chloroplasts, Animal cells do not.

Explanation:

The correct answer is a,

What are two tools used for sending information? cars and buses water and wind smoke and wood radio waves and lightwaves

Answers

Answer:

radio waves and lightwaves

Explanation:

Radio waves and light waves are both types of electromagnetic waves in the electromagnetic spectrum. Radio waves and light waves travel a particular distance and hence, are used to convey information over distances.

Based on this question, radiowaves and light waves are both tools used to send information. Radiowaves carry information from the station to the receiver, likewise light waves from one medium to another.

what color is blood when it is in your blood

Answers

Answer:

red if it has oxygen, but blue when it doesnt

Explanation:

Answer:

Red or blue depending if it has oxygen or not

Explanation:

Please help I’ll mark brainliest

Answers

Answer:

mark me brainliest

Explanation:

I think that it’s B, water movement into the cell is dependent on the percent concentration of solutes both inside and outside the cell

One reason male peacocks spread their tail feathers is to
attract a mate. How does choosing a male with a bright,
full tail increase a peahen's chance of producing healthy
peachicks?

Answers

Answer:

the appearance of the peacock shows he is healthy, and if the peahen mates with him, it is likely that their offspring will also be born healthy. you would want to reproduce with the fittest individuals of your species and guarantee your species will continue to thrive.

Explanation:

What is the best way to record observations related to growth over time?

Answers

A line graph shows change over time.

Can someone help me please

Answers

Answer:

Cellular respiration

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