Two different enzymes catalyze the same reaction and both exhibit the same Vmax. When Enzyme A was run with a 40 uM substrate, the initial rate (Vo) was 10 uM/min, and when g it was run with a 4 mM substrate, the Vo was 20 uM/min. Estimate the approximate Vmax and Km of Enzyme A. When Enzyme B was run with 80 uM substrate, the initial rate (Vo) was 10 uM per minute. Estimate the Km of Enzyme B.

Answers

Answer 1

Answer:

a) k_m = 4.08 uM

  V_{max} = 20.07 uM/min

b) k_m = 8.16 uM

Explanation:

Given that:

For Enzyme A:

the substrate concentration [S] = 40 uM

the initial velocity rate v = 10 uM/min

when it was 4mM, v = 20 uM/min

i.e.

at 4mM = 4000 uM;

Using Michealis -menten equation;

when v = 10

[tex]V = \dfrac{V_{max}[S]}{k_m+[S]}[/tex]

[tex]10 = \dfrac{V_{max}\times 40}{k_m + 40}[/tex]

[tex]10 (k_m + 40) = V_{max}40[/tex]

[tex]40V_{max} -10k_m = 400 --- (1)[/tex]

when v= 20

[tex]20= \dfrac{V_{max}\times 4000}{k_m + 4000}[/tex]

[tex]20 (k_m + 4000) = V_{max}4000[/tex]

[tex]4000V_{max} -20k_m = 8000 --- (2)[/tex]

equating equation (1) and (2):

[tex]40V_{max} -10k_m = 400 --- (1)[/tex]

[tex]4000V_{max} -20k_m = 8000 --- (2)[/tex]

let multiply equation (1) by 100 and equation (2) by 1

4000V_{max} - 1000K_m = 4000

4000V_{max} - 20 k_m = 8000    

  0        -980k_m = 4000

k_m = 4000/-980

k_m = 4.08 uM

replacing the value of k_m into equation (1)

40{V_max } - 10(4.08) = 400

40{V_max } - 40.8 = 400

40{V_max } = 400 +  40.8

40{V_max } = 440.8

V_{max} = 440.8/40

V_{max} = 11.02 uM/min

b)

Since V_{max} of A ie equivalent to that of B; then:

V_{max} of B = 11.02 uM/min

Here;

[S] = 80  uM

V = 10 uM/min

[tex]10 = \dfrac{11.02 \times 80}{k_m + 80 }[/tex]

10(k_m +80) = 881.6

10k_m = 881.6  - 800

10k_m = 81.6

k_m = 81.6/10

k_m = 8.16 uM


Related Questions

Earth's biosphere is described as

Answers

the zone of life. The sum of all life on Earth.  

The biosphere is sometimes thought of as one large ecosystem—a complex community of living and nonliving things functioning as a single unit. More often, however, the biosphere is described as having many ecosystems. People play an important part in maintaining the flow of energy in the biosphere.


3.3
Predict the possible genotypes of offspring if two roan heterozygous
polled cattle were mated​

Answers

Answer:

RR, Rw, Rw, ww

Explanation:

Rw

R

w

RR, Rw, Rw, ww

The phenotypes would be 1 Red, 2 Roan, and 1 white, assuming the colors of roan are white and red and red is dominant white is recessive, hope that helps!

What is the
nature of water molecules makes that able to absorb heat and moderate our
climate.

Answers

the answer is hydrogen bonds i’m pretty sure. if not i’m sorry!!:)

which consumers live in neritic zone

Answers

Answer:

crabs, shrimp, starfish, scallops and sea urchins

Explanation:

Explain why you would recommend the nguni cattle meat to consumers in your locality.​

Answers

Answer:

Below

Explanation:

All different combinations were catalogued in the beginning of the century by a South African herdmaster. This work inspired the Nguni Cattle Register, a compilation of terms to describe in full a Nguni cow or bull. The cattle are medium-sized, with bulls weighing between 500 and 600 kg,[3] while cows weigh between 300 and 400 kg.

A researcher crossed two plants, and informed an assistant researcher to determine the genotypes and phenotypes of the plants that were crossed by analyzing the offspring. The assistant counted 30 plants with green pods and 10 plants with yellow pods. Which of the following conclusions can accurately be made by the assistant?

a. The researcher had selfed a true-breeding plant with green pods.
b. The researcher had crossed true-breeding plants of differing pod colors.
c. The researcher had crossed two heterozygous plants.
d. The researcher had crossed two plants with yellow pods.
e. The researcher had crossed a plant with green pods to a plant with yellow pods.

Answers

Answer:

c. The researcher had crossed two heterozygous plants.

Explanation:

Due to technical problems, you will find the complete explanation in the attached files.


What was one major effect of urbanization on British society during the
Industrial Revolution?
O A. Urban citizens were forced to drink unsanitary and polluted water.
B. Improved living conditions resulted in a decline in the mortality
rate.
O C. Governments passed strict regulations to reduce the levels of
pollution.
D. Wealthy corporations relocated their factories to rural areas.

Answers

Answer:

B is the answer

B. Improved living conditions resulted in a decline in the mortality

rate.

Hope it helps

The major effect of urbanization is improved living conditions resulted in a decline in the mortality rate during industrial revolution.

What is industrial revolution?

The Industrial Revolution was the shift to new industrial methods in the United Kingdom, continental Europe, and the United States from around 1760 until between 1820 and 1840.

The major effect of urbanization is improved living conditions resulted in a decline in the mortality rate during industrial revolution.

Thus, the correct option is B.

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8. Which example describes a consumer?
A. an organism that makes its own food
B. an organism that eats other organisms
C. an organism that breaks down dead material for food

Answers

Answer:

your answer is probably gonna be A

Answer:

B. An organism that eats other organisms

Explanation:

paste or draw pictures of five junk food items and list five harmful effects of those items

Answers

Junk food burger pizza
1. Chocolate- Very harmful as it contains high sugar which can result in risk factor of many diseases and put on excessive weight. They also contain much caffeine which can damage our body and cause migraines.
2. Chips- They are bad for you as they contain many unknown chemicals which can increase possibility of heart attacks and cancer.. they do not contain much nutrients and build up fat
3. Soda- Contains a lot of sugar and causes multiple excessive conditions while gaining some diabetic disorders.
4. Burgers- they contain too much sodium which is a large number to cause a disaster of indigestion. They also increase the cholesterols in your body.
5. Ice cream- Contains artificial flavors and usage of different ingredients which may be safe for the body. Also there is many calories and sugar stacked.

A plant can have green (G) or yellow (g) leaves. It can also have a long (K) or
short (k) stem. A scientist is preparing a Punnett square for a dihybrid cross
of a plant with a genotype GGKk. What possible gametes can the plant
produce?

Answers

Here ya go hoope this helps

Without replication, organisms could not successfully


move.

grow and reproduce.

feed.

make proteins.

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Answer: grow and reproduce

Explanation:

Pharmacological management of glaucoma focuses on the reduction of intraocular pressure by increasing the drainage of aqueous humor, decreasing the production, or both. What drug classes are used to reduce intraocular pressure

Answers

Answer:

Beta blockers.

Explanation:

Beta blockers are the drugs that blocks the effects of the hormone adrenaline. Beta blockers is responsible for slowing of your heart beat more which ultimately reduce blood pressure. Beta blockers  are the drugs which reduce the production of fluid in your eye, thereby lowering the pressure in your eye means intraocular pressure. Examples of beta blocker medicines are timolol (Betimol, Istalol, Timoptic) and betaxolol (Betoptic).

If Mendel had been working with his pea plants and noticed that not all purple flowers had an equal intensity of purple, what is a likely explanation for the difference?

A. variations in cell signaling driving differences in gene expression

B. variations in the weather

C. variations in how the seed was handled

D. variations in the cell walls

Answers

The Answer is either (A) Variations in cell signaling driving differences in gene expression OR (D) Variations in the cell walls

You are a research biologist working at the U.S. research station in Antarctica. You discover a new single-celled organism that is able to survive in the frigid conditions. During your research you observe that the organism has a nucleus. Which domain would this new organism be classified into

Answers

Answer: Eukarya.

Explanation:

The cell is the functional and morphological unit of all living beings. Organisms can be classified as follows: if they are composed of a single cell, they are called unicellular (such as protozoa or bacteria which are microscopic organisms), or mutlicellular if they have more cells.

The three-domain system is a biological classification into three groups:

Archaea Bacteria Eukarya.

It is based on the differences found in the sequence of the ribosomal RNA of the minor subunit to conclude that these groups developed separately from a common progenitor.      

Archaea are a group of unicellular prokaryotic microorganisms that have no nucleus or any internal membranous organelles. However they are different from bacteria, so that they form their own domain.

Bacteria are prokaryotic microorganisms that do not have a defined nucleus nor do they generally have internal membranous organelles.

Although archaea and bacteria are morphologically very similar, archaea are closer to eukaryotes in some genes and pathways of transcription and translation. Thanks to studies of genetic material, now we knot that the evolution of archaea and bacteria had been independent, so archaea could no longer be considered as a group of bacteria. However, the Archaea and Bacteria domains include prokaryotic organisms, i.e. those whose cells do not have a differentiated cell nucleus.

The Eukarya domain includes the best known and most complex forms of life (protists, animals, plants and fungi). It includes organisms formed by cells with a true nucleus. These organisms consist of one or more eukaryotic cells, ranging from unicellular to multicellular organisms in which the different cells are specialized for different tasks and which, in general, cannot survive in isolation.

So, if the observed organism possesses a nucleus, then it is a eukaryote.

Short Answer Questions
What TWO things are necessary for evolution by natural selection to
occur?

Answers

Selective pressure and an adaptation. Having the adaptation allows the organism to survive long enough to pass their genes. Without a selective pressure, the adaptation would just be a trait

Where Adam and Eve really the first people? or where there other people before them, or did adam and eve even exist at all. explain your reasoning.

ᴵ ˡᶦᵏᵉ ᵗᵒ ˢᵉᵉ ʷʰᵃᵗ ᵒᵗʰᵉʳ ᵖᵉᵒᵖˡᵉ ˢᵃʸ ᵃᵇᵒᵘᵗ ᵗʰᶦˢ.
i will give brainly if it is convencing.

Answers

Answer:

If you are religious then you might believe in Adam and Eve and the Garden of Eden. But if you believe in science, then you would believe in evolution.

Sapiens or humans have been on the Earth for around 300,000 years.

Earlier ancestors existed 1.4 to 2 millions years ago.

Earliest writing is 5400 years old.

The bible is 2700 years old.

Why was there no record of Adam and Eve earlier?

Because they never really existed until someone created them.

The someone wasn't a deity . It was a man. A man who created a story.

during chemosis what substance diffuse from one side to other side of the membrane​

Answers

Answer:

During chemosis,transport chain substance diffuse from one side to other side of the membrane.

the answer of what is matter ​

Answers

Answer:

noun

noun: matter; plural noun: matters; noun: the matter

1.

physical substance in general, as distinct from mind and spirit; (in physics) that which occupies space and possesses rest mass, especially as distinct from energy.

"the structure and properties of matter"

a particular substance.

"organic matter"

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material

substance

stuff

medium

written or printed material.

"reading matter"

2.

a subject or situation under consideration.

"a great deal of work was done on this matter"

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affair

business

proceeding

situation

circumstance

event

happening

occurrence

incident

episode

occasion

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question

point

point at issue

item

case

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theme

LAW

something which is to be tried or proved in court; a case.

the present state of affairs.

"we can do nothing to change matters"

3.

the reason for distress or a problem.

"what's the matter?"

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problem

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the substance or content of a text as distinct from its style or form.

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Trust me mark me as brainliest trust me

ONE FOOD CHAIN that was affected by the introduction of wolves and model it

Answers

Answer:

Carnivorous

Explanation:

The wolf, also known as the gray wolf or grey wolf, is a large canine native to Eurasia and North America. More than thirty subspecies of Canis lupus have been recognized, and gray wolves, as colloquially understood, comprise non-domestic/feral subspecies.

4. Which statement is true of all biotic factors?
A. They are alive
B. They are renewable.
C. They use photosynthesis.
D. They use chemosynthesis.

Answers

Answer:

option a

Explanation:

Answer:

The answer is A

Explanation:

Biotic factors are living organisms in the ecosystem.Examples are animals,birds,fungi

Watch this video about a river that China is building. Explain how the river will help prevent the negative effects of drought. In your answer, explain any negative effects that you foresee this new river will have on the people or on natural habitats.

Answers

Answer: The river will increase Beijing’s water supply, which is currently at risk of not meeting the city’s needs. This increase will help to soften the effect of droughts.

The downside of the new river is that it may disrupt natural areas, including habitats for plants and animals. The project will also be expensive and may take up space that could be used for homes, businesses, or agriculture.

Explanation: Change the wording...

The construction of a new river can help prevent the negative effects of drought by increasing the availability of water for human and ecological needs, and one way that a new river can help prevent the negative effects of drought is by increasing the recharge of groundwater resources.

What is a drought?

During periods of drought, water resources can become scarce, leading to reduced crop yields, drinking water shortages, and negative impacts on ecosystems. By providing a new source of water, a new river can help alleviate some of these negative effects. A new river can provide a source of water that can help recharge aquifers and replenish groundwater supplies. However, the construction of a new river can also have negative effects on people and natural habitats. One potential negative impact is the displacement of people living in the area where the river is being constructed. This can lead to the loss of homes, farms, and livelihoods for local communities.

Hence, the construction of a new river can help prevent the negative effects of drought by increasing the availability of water for human and ecological needs.

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Complete each sentence with the correct word or phrase

a. An action potential reaches the axon terminal causing __________to open.
b. Acetylcholine diffuses across the synaptic cleft before binding to its receptor, which is located on the _____
c. When the membrane potential reaches ________an action potential spreads across the sarcolemma due to the opening of ___________
d. In response to acetylcholine binding, ________ move into the muscle fiber causing the membrane potential to rise.

Answers

Answer:

a). Voltage-gated Ca2+ channels

b). the motor end plate

c). Threshold, voltage-gated Na channels

d). sodium ions

Explanation:

a). The voltage-gated [tex]Ca^{2+}[/tex] channels are the key transducers of the membrane potential that changes into a intracellular [tex]Ca^{2+}[/tex] transients which initiates the  physiological events. When the action potential makes a contact with axon terminal, the voltage-gated [tex]Ca^{2+}[/tex] channels opens.

b). The acetylcholine is the main neurotransmitter of the parasympathetic nervous system. It helps in blood vessels dilation, muscle contraction, and perform many more functions.

It diffuses with the synaptic cleft that is located on the motor end plate of the nervous system.

c). The membrane potential is also known as the membrane voltage or the  transmembrane potential. When it recaches the threshold, the  voltage-gated Na channels opens to spread the action potential across sarcolemma.

d). The sodium ions in case of acetylcholine binding moves to the muscle fiber due to which the membrane potential rises.

How were the governments of Japan and Italy similar in the 1930s? Check all that apply.

Both were headed by groups of military leaders.
Both used extreme nationalism to win support.
Both were supported by citizen armies.
Both began programs of aggressive expansion.
Both were known as militarist governments.

Answers

Answer:

The correct answers are:

B. Both used extreme nationalism to win support.

D. Both began programs of aggressive expansion.

The governments of Japan and Italy similar in the 1930s because they both used extreme nationalism to win support and began programs of aggressive expansion.

Explanation:

Both governments (Japan and Italy) used extreme nationalism to win support and both began programs of aggressive expansion.

Similarity between government of Japan and Italy in 1930

The governments of Japan with its territory in Asia had a similarity in government with that of government of Italy located in Europe.

In 1930 both governments (Japan and Italy) used extreme nationalism to win support in order to have more allies and they also began programs of aggressive expansion to increase their colonies.

Thus, the correct options include, both used extreme nationalism to win support and both began programs of aggressive expansion.

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1. What is stimulus?

2. Define response.

3. What are tropic movements
4. What excretory products are produced in plants?

5. When do stomata open and close?

Answers

Answer:

1.someting that causes directly, development, interest.

2.an answer to somebody something

3.directional movement of particular part of a plant in response to environmental stimuli

4. carbon dioxide, oxygen

5. allow for the uptake of carbon dioxide anf to limit the loss of water due to evaporation

I NEED ANSWERS ASAP!!​

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A jehsjxnsbfgviyfvhhugjvhugv

Factors determine energy a human being requires in a day

Answers

Human energy requirements are estimated from measures of energy expenditure plus the additional energy needs for growth, pregnancy and lactation. Recommendations for dietary energy intake from food must satisfy these requirements for the attainment and maintenance of optimal health, physiological function and well-being. The latter (i.e. well-being) depends not only on health, but also on the ability to satisfy the demands imposed by society and the environment, as well as all the other energy-demanding activities that fulfil individual needs.

Energy balance is achieved when input (i.e. dietary energy intake) is equal to output (i.e. total energy expenditure), plus the energy cost of growth in childhood and pregnancy, or the energy cost to produce milk during lactation. When energy balance is maintained over a prolonged period, an individual is considered to be in a steady state. This can include short periods during which the day-to-day balance between intake and expenditure does not occur. An optimal steady state is achieved when energy intake compensates for total energy expenditure and allows for adequate growth in children, and pregnancy and lactation in women, without imposing metabolic, physiological or behavioural restrictions that limit the full expression of a person’s biological, social and economic potential.

Within certain limits, humans can adapt to transient or enduring changes in energy intake through possible physiological and behavioural responses related to energy expenditure and/or changes in growth. Energy balance is maintained, and a new steady state is then achieved. However, adjustments to low or high energy intakes may sometimes entail biological and behavioural penalties, such as reduced growth velocity, loss of lean body mass, excessive accumulation of body fat, increased risk of disease, forced rest periods, and physical or social limitations in performing certain activities and tasks. Some of these adjustments are important and may even increase the chances of survival in times of food scarcity.

Z 00m 336"083"2553 (wZE2XQ)​

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Describing the formation of the Solar system

Answers

The Sun and the planets formed together, 4.6 billion years ago, from a cloud of gas and dust called the solar nebula. A shock wave from a nearby supernova explosion probably initiated the collapse of the solar nebula. The Sun formed in the center, and the planets formed in a thin disk orbiting around it

How do somatic and Inherited mutations differ?
O A. Somatic mutations exist in all cells in the body and Inherited mutations only exist In the cell type that mutated.
O B. Inherlted mutations exist in all cells in the body and somatic mutations only exist In the cell type that mutated.
O C. Somatic mutations only affect males and inherited mutations affect any gender.
O D. Inherited mutations only affect males and somatic mutations affect any gender.

Answers

The right option is; B. Inherited mutations exist in all cells in the body and somatic mutations only exist in the cell type that mutated.
Inherited mutations are gene mutations that exist in almost every cell in the body throughout an individual’s life. Inherited mutations are inherited from a parent and can also be transmitted. They are also called germline mutations because they exist in the parent’s egg or sperm cells (gametes).
Somatic mutations are gene mutations that exist in only certain cell types that are mutated, not in every cell in the body and it take place at some time during an individual’s life. Somatic mutations are not inherited and cannot be passed. They are caused due to environmental factors (such as cigarette smoke or ultraviolet (UV) radiation) or due to error during DNA replication.

Match the structural formula to the chemical formula for this substance.

но-с-с-он
ІІ.

C2H2(OH)2
ООО
HOC2OH
С2Н6O2
O H4CO3H2

Answers

Answer:

C2H6O2

Explanation:

Just did it on edge...

Answer:

C2H6O2

Explanation:

Edge 2021

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