The bacterium Methylophilus methylotrophus can use methanol and ammonia for protein synthesis. The glutamate dehydrogenase gene from E. coli was introduced into M. methylotrophus using recombinant DNA techniques. Under high ammonia conditions, the introduction of the glutamate dehydrogenase gene into M. methylotrophus resulted in an increase in protein yield. Select the statements that are reasonable explanations for the increased protein yield in the modified M. methylotrophus. Glutamate dehydrogenase catalyzes the formation of glutamate and glutamine in a two-step process. Both glutamate and glutamine play a key role in amino acid synthesis, thus an increase in levels of glutamate and glutamine increase protein synthesis. Glutamate dehydrogenase has a low affinity for NH^+_4, and can only catalyze the formation of glutamate from alpha-ketoglutarate and NH^+_4, when the concentration of NH^+_4 is high. Glutamate dehydrogenase has a high affinity for NH^+_4, thus the rate of glutamate synthesis increases exponentially under high ammonia conditions. Glutamate is the amino group donor for most transamination reactions involving amino acids, thus an increase in glutamate concentration increases protein synthesis.

Answers

Answer 1

Answer:

- Glutamate dehydrogenase has a low affinity for NH+4, and can only catalyze the formation of glutamate from alpha-ketoglutarate and NH+4, when the concentration of NH+4 is high.  

- Glutamate is the amino group donor for most transamination reactions involving amino acids, thus an increase in glutamate concentration increases protein synthesis.

Explanation:

Glutamate dehydrogenase (GLDH) is an enzyme found in prokaryotic and eukaryotic cells, which is known to catalyze the reversible oxidative deamination of glutamate (Glu) to alpha-ketoglutarate and ammonia at the same time the oxidation of NADH to NAD. In bacteria, this enzyme (GLDH) is efficient only at high concentrations of the ammonium cation (NH4+), because it has a low affinity for NH4+. Moreover, transamination is a pathway that involves the transfer of an amino group from one amino acid to a ketoacid in order to form new amino acids (this reaction is responsible for the deamination of most amino acids). Glu is the donor of amino groups in most of the reactions catalyzed by enzymes involved in the transamination between an amino acid and an alpha-keto acid. In consequence, in presence of GLDH, a high concentration of Glu will increase the production of new amino acids and therefore also protein synthesis.


Related Questions

If the initial ratio of 14C to 12C in the bones was one part per trillion (the current ratio of 14C to 12C in the atmosphere), approximately what ratio would we expect in bones that come from wolves trapped 28,500 years ago

Answers

Answer:

For the bones of wolves trapped 28,500 years ago, the approximate ratio of carbon-14 to carbon-12, 14C to 12C, will be one part per 32 trillion.

Explanation:

Radioactive isotopes of elements are useful for many purposes. Carbon has three isotopes, carbon-12, carbon-13, and carbon-14. Of these,  carbon-14 is radioactive.

The half-life of an carbon-14 is used to determine the age of fossils in a process known as carbon-dating.

Carbon-14 has a half-life of approximately 5700 years. The half-life being the time for half the amount of the radioactive nuclei present in a radioactive element to decay.

The number of half-lives in 28500 years  = 28500/57000 = 5 half-lives

This means that the original amount of atoms present in the atoms has undergone radiactive decay five times.

The ratio of the original amount of 14C to the present amount of 14C = (1/2)⁵ = 1/32

Ratio of 14C to 12C that will be found in the bones = 1/32 × 1/ 1 trillion = 1/32 trillion.

Therefore, for the bones of wolves trapped 28,500 years ago, the approximate ratio of carbon-14 to carbon-12, 14C to 12C, will be one part per 32 trillion.

DNA contains four types of nitrogenous bases .One of these bases is -

Answers

Answer:

adenine (A), cytosine (C), guanine (G), and thymine (T)

animal modified for climbing are called ​

Answers

Arboreal animals are those animals that spend most of their life on trees. They feed, travel, play, and sleep on trees.

Please help!! Im gussing it’s either Y or Z

Answers

Answer:

The answer is Y

Explanation:

Trust me I've done this before

Reactants definition

Answers

the substance that is getting changed / affected by another chemical substance

economic importance of mosquito larva list five​

Answers

Explanation:

economic importance of mosquito larva are

Mosquito larvae grow by consuming microorganisms such as algae and microbes that decompose decaying plant material. Larval mosquitoes contribute to aquatic food chains by serving as food sources for many predators, including fish and birds.i hope it will help you

A population has adopted an evolutionarily stable strategy (ESS) in regards to cooperation or defection. If a new mutant strategy appears that causes an opposite behavior from the norm, which of the following will result?
A. The mutant strategy will not be able to increase in numbers in the population under the influence of natural selection.
B. Antagonistic pleiotropy will allow an equilibrium in which both strategies are maintained in the population.
C. The members of the population using the original strategy will act with aggression toward those exhibiting the mutant strategy.
D. The evolutionarily stable strategy will be displaced because of natural selection in favor of the mutant strategy.
E. Natural selection will maintain the mutant strategy because the variation in strategy may be useful in future environments.

Answers

Answer:

A. The mutant strategy will not be able to increase in numbers in the population under the influence of natural selection.

Explanation:

An evolutionarily stable strategy (EES) can be defined as a strategy that is competitively superior to others strategies and cannot be replaced or invaded by any other strategy through natural selection, resulting in punishment for those who deviate from it. Thus, an EES may confer a higher reproductive/adaptive fitness than alternative strategies when it is adopted by most members of the population. Individuals having an ESS are 'spiteful' because they act not only to increase their payoffs but also to decrease the payoffs of their competitors. An EES is resistant to low levels of mutation when all individuals in the population have the same strategy. In game-theoretical terms, an ESS is a strategy such that if all the members of a population adopt this strategy, then no mutant strategies could invade the population under natural selection. In consequence, when all individuals of a population adopt an EES, mutant phenotypes cannot invade and displace the ESS.

Complete each statement by using the appropriate word or phrase from the list. Then place the statements in the order of an action potential repolarization event.
1. Through the activated channels, _________ now flows outwards causing the membrane to repolarize back towards RMP.
2. As the membrane potential ___________, the voltage-regulated sodium channels begin closing which in turn reduces the sodium movement to the ICF
3. With a larger duration of activation than sodium channels, the potassium channels allow for a greater movement of potassium to the ECF causing a short period of_______.
4. With membrane potentials approaching +35 mV, the _________channels become fully activated.
a. Sodium
b. Voltage regulate sodium
c. Potassium
d. Rises towards zero
e. Hyperpolarization
f. Voltage regulated potassium

Answers

Answer:

1. Potassium

2. increasing towards zero

3. hyperpolarization

4. voltage-regulated Potassium

Explanation:

Membrane potential can be defined as the difference in electric charges inside and outside of a cell. The resting membrane potential (RMP) occurs when there is no net current across the membrane and therefore the cell is in a non-excited state. At the RMP, sodium ions (Na+) are more concentrated inside the extracellular fluid (ECF) than inside the intracellular fluid (ICF), while potassium ions (K+)  are more concentrated inside the ICF. The diffusion of K+ outside the cell triggers its hyperpolarization, by becoming the membrane potential more negative compared to the resting potential. As the potential nears +35 mV, the voltage-regulated potassium channels are open, thereby K+ ions leave the cell down its concentration gradient, while voltage-gated Na+ channels become saturated and inactivate.

What is a population?
A. a group of organisms of different species interacting with each other and the environment
B. a single separate organisms separate from others of the same kind interacting with it's environmment
C.all the organisms of the same species who live in a specific environment and are capable of interbreeding.
D.a group of organisms and the non-living components interacting as a system

Answers

Answer:

What is population?

A group of organisms of different species interacting with each other and the environment is called populatin.

Suc- strains cannot utilize sucrose, trp- strains cannot synthesize tryptophan. In the generalized transduction of a suc-trp- strain of E. coli using bacteriophage derived from a suc+trp+ cell, what medium would select for suc+ and trp+?
A) a minimal medium
B) a minimal medium + sucrose
C) a minimal medium + tryptophan
D) a minimal medium + sucrose + tryptophan
E) none of the above

Answers

Answer:

B) a minimal medium + sucrose

Explanation:

Suc+ ⇒ can utilize sucrose, so you have to add sucrose to the medium.

Trp+ ⇒ can synthesize tryptophan, so it is not required to add tryptophan to the medium because the cell can produce it.

According to this, to select for suc+ trp+ you have to only add sucrose to the medium. Thus, it will only grow the cells that can utilize sucrose and can synthesize tryptophan.

Therefore, the correct option is: B) a minimal medium + sucrose

Which of the following is true about evaluating an experiment?

A)Evaluating an experiment allows the investigator to find errors and make improvements.
B)Evaluating an experiment is not important or required.
C)Evaluating an experiment can change the prediction.
D)Evaluating an experiment can make it more confusing.

Answers

Answer:

The correct answer is - A) Evaluating an experiment allows the investigator to find errors and make improvements.

Explanation:

Evaluation is the process in any experiment that helps in improving the analysis and remove missing components and errors to get better results. It also checks if all the process is included and running properly without error.

One should constantly be evaluating his or her experiment. It includes assessing how the experiment is going as you carry it out, what could have been improved if it was to be carried out again and how can eliminate any erros.

Which gas is exhaled along with used air?




SCIENCE QUESTION... ☺️​

Answers

Explanation:

When we take a breath, we pull air into our lungs that contains mostly nitrogen and oxygen. When we exhale, we breathe out mostly carbon dioxide.

What are the two layers of the skin and the 1 layer below the skin?

Answers

Skin has three layers: The epidermis, the outermost layer of skin, provides a waterproof barrier and creates our skin tone. The dermis, beneath the epidermis, contains tough connective tissue, hair follicles, and sweat glands. The deeper subcutaneous tissue (hypodermis) is made of fat and connective tissue.07-Aug-2019

DNA is transcribed into RNA, then RNA is translated into amino acids we can build into proteins

Answers

During transcription, the enzyme RNA polymerase (green) uses DNA as a template to produce a pre-mRNA transcript (pink). The pre-mRNA is processed to form a mature mRNA molecule that can be translated to build the protein molecule (polypeptide) encoded by the original gene.

9. What is the relationship between natural selection and evolution?​

Answers

Answer:

Through this process of natural selection, favorable traits are transmitted through generations. Natural selection can lead to speciation, where one species gives rise to a new and distinctly different species. It is one of the processes that drives evolution and helps to explain the diversity of life on Earth.

Explanation:

Natural selection is a process where organisms that are better adapted to an environment will survive and reproduce. This means that the advantageous alleles of this variant organism are passed on to offspring. Over many generations, the process of natural selection leads to evolution occurring.

How do organisms have a negative impact on the water cycle? And how can they affect other organisms with your answer? Please help.

Answers

Too much of one organism can cause an imbalance in the habitat because of its niche occupation

số lượng protein thu được là bao nhiêu nếu tổng hợp phân tử protein chứa 100aa, trong mỗi phân tử đều chứa 20aa khác nhau

Answers

Explanation:

feg3fegrgt✴☝️☝️☝️☝️⛲

Answer:

protein number of collect is a bao nhiêu nếu protein phân tử tổng hợp chứa 100aa, trong mỗi phân tử đều chứa 20aa khác nhau

Explanation:

->1 phân tử chứa đủ 20 aa vậy ta cần 5 protein để tổng hộp thành 100aa

Match each of the following skull features with its correct bone.

a. styloid process
b. infraorbital foramen
c. sella turcica
d. foramen magnum
e. coronoid process
f. crista galli

1. ethmoid bone
2. sphenoid bone
3. temporal bone
4. mandible
5. maxilla
6. occipital bone

Answers

Answer:

The left column contains structures of the bones that belong to the right column.

a) styloid process - 3) temporal bone

b) infraorbital foramen - 5) maxilla

c) sella turcica - 2) sphenoid bone

d) foramen magnum - 6) occipital bone

e) coronoid process - 4) mandible

f) Crista galli - 1) ethmoid bone

Explanation:

The inferior part of the petrous temporal bone forms the styloid process that allows the stylohyoid, stylopharyngeus, and styloglossus muscles to attach with styloid ligaments.

The infraorbital foramen is the opening of the maxillary bone of the skull

sella turcica is a saddle-like structure in the body of the sphenoid bone of the skull.

The foramen magnum is the lower part of the cranial  foss that allows the connecting brain and spinal cord and it the opening

The coronoid process is shoulder joint as well and required mandibles in the skull to form.

Crista galli arises from ethmoid bone that is thick and midline triangular but smooth process.

Which factors improve soil fertility? Select the three correct answers.

A. Nitrogen
B. Humus
C. Manure
D. Horizons
E. Bedrock​

Answers

A.Nitrogen
B.Humus
c.Manure
hope this helps

Molecules produced in the nucleus are transported to the cytoplasm via?​

Answers

Answer:

Molecules produced in the nucleus are transported to the cytoplasm via endoplasmic reticulum.

The Earth and the moon are kept in orbit by gravity and mass. Please select the best answer from the choices provided T F

Answers

Answer:

True

Explanation:

the Earth and the Moon are kept in orbit by gravity and mass.

Answer:

True (T)

Explanation:

Yeah, the Earth and the moon are kept in orbit by gravity and mass. Hence, this statement is True (correct).

Question 3 of 10
What is the next step in the scientific method, following stating a question?
A. Analyzing the data
B. Forming a hypothesis
C. Collecting the data

Answers

Answer:

B. Forming a hypothesis

Explanation:

Once you have a question in mind, you form a hypothesis (which is sort of an educated guess of what you expect or do not expect to happen) and then finally you can start on scientific investigations of collecting data and end with analyzing data and showing conclusion.

Answer:

the correct answer is B

Explanation:

because you always try to understand your question after you state it

Look at the picture of a leaf-cutter ant.
Based on this picture, which prediction can be made about leaf-cutter ants?
A. They eat trees for energy.
B. They produce hundreds of offspring at a time.
C. They cannot see color.

Answers

Answer:

The Answer is -They are strong enough to lift an entire leaf

Explanation:

Based on the picture of a leaf-cutter ant. The prediction that can be made about leaf-cutter ants is they are strong enough to lift an entire leaf. The correct option is D.

What is a leaf-cutter ant?

Leaf-cutter ants develop their fungus crop by using leaves as fertilizer. By providing freshly cut leaves, they develop fungal gardens while guarding against pests and molds and removing dead materials. The fungus serves as a food supply for the ant larvae in exchange.

These eager ants may seem to bite off more than they can chew, but in reality, they are capable of carrying leaf fragments that are nearly 50 times heavier than themselves. That's like a person pushing a car around.

Therefore, the correct option is D. they are strong enough to lift an entire leaf.

To learn more about leaf-cutter ants, refer to the link:

https://brainly.com/question/29309882

#SPJ2

it is responsible for being bidirectional of the cell membrane​

Answers

Answer:

I believe it is Cholestral

Explanation:

ở một loài thực vật, xét 2 cặp gen phân li độc lập A,a và B, b, mỗi gen quy định 1 tính trạng, alen trội là trội hoàn toàn. Tiến hành mmotj phép lai giữa hai cây, thu được F1. Thep lý thuyết, kiểu hình mang hai tính trạng trội ở F1 không thể chiếm tỷ lệ:
A: 12,5% B: 37,5%

Answers

I am sorry I just wanted points for my answer and I am sorry again I needed points

Which type of RNA functions as the location where proteins are made? mRNA rRNA tRNA siRNA

Answers

Answer: rRNA

Explanation: mRNA is the messenger RNA (sequence determined by DNA) and it exits the nucleus and meets the tRNA (transfer RNA) in the ribosome or more specifically the rRNA (answer). I’m not sure what siRNA is, but I’m 99% sure the answer is rRNA.

The type of RNA that functions as the location where proteins are made would be the rRNA.

rRNAs are known as ribosomal RNAs. They are usually found associated with the ribosome - the organelles responsible for synthesizing proteins in eukaryotic cells.

mRNA is the messenger RNA. They are formed directly from the transcription of DNA in the nucleus and then transported to the cytoplasm. They carry genetic information in coded forms and the codes are translated protein synthesis.

tRNA is the transfer RNA. It carries amino acids and anticodons.

siRNA are small interfering RNA.

More on the RNAs can be found here: https://brainly.com/question/19815744

how does fertilization of angiosperm differ from fertilization in bryophytes​

Answers

Answer:

Fertilization in flowering plants happens through a process called pollination. Pollination occurs when pollen grains from the anther land on a stigma. ... Fertilization occurs when one of the sperm cells fuses with the egg inside of an ovule. After fertilization occurs, each ovule develops into a seed

Proteus vulgaris has a doubling time of roughly 28 minutes. If an initial population of 500 cells is allowed to grow for 6 hours in ideal conditions, what will be the final population? Use three significant figures for your final answer.

Answers

Answer: The final population of Proteus vulgaris after 6 hours is [tex]3.71\times 10^{6}cells[/tex]

Explanation:

We are given:

Proteus vulgaris divides and doubles every 28 minutes

Total time given = 6 hours = 360 min      (Conversion factor: 1 hr = 60 min)

Number of times Proteus vulgaris doubles in 6 hours = [tex]\frac{360min}{28min}=12.857times[/tex]

Calculating the number of bacteria after 6 hours under ideal conditions:

We are given:

Initial population = 500 cells

Number of times it doubles = 12.857 times

Final Proteus vulgaris population = [tex]500\times 2^{12.857}=3709476.8=3.71\times 10^{6}cells[/tex]

Hence, the final population of Proteus vulgaris after 6 hours is [tex]3.71\times 10^{6}cells[/tex]

How do plant cells that do not have chlorophyll get food?​

Answers

Answer:

All plants cells have chlorophyll so there is no problem

Record the disorder revealed in the karyotype below.
I need help

Answers

Answer:

i don't have that alien eys

you do have

in one word this photo is not clear for my eyes

[tex]1 \sqrt{ \frac{ \frac{24 \frac{ \frac{21150 \sqrt{ \cot( \alpha ) } }{?} }{?} \times \frac{?}{?} \times \frac{?}{?} }{?} }{?} } [/tex]

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