You decide to perform a chromatin immunoprecipitation to see whether your favorite gene, the Cool gene, is associated with H3 histones that are methylated at lysine 9 (on the histone tail).
You then want to determine whether this methylation also means that expression of the Cool gene is inhibited in cells.
1. Which of the following techniques will allow you to answer this question?
O Northern blot
O DNA footprinting
O Yeast 2 Hybrid
O Quantitative RT-PCR
O Microaray
O Hybridization

Answers

Answer 1

Answer:

Quantitative RT-PCR

Explanation:

Reverse transcription polymerase chain reaction (RT-PCR), also known as quantitative Real-Time (RT) PCR, is a technique widely used in molecular biology laboratories in order to assess the expression level of target genes. This technique employs a transcriptase inverse that enables to obtain DNA from total RNA and then this total DNA is amplified as in a Polymerase Chain Reaction. The levels of the target genes are obtained by comparing their relative expression with one or more control genes.


Related Questions

While examining a layer of rock, a scientist found a fossil of a trilobite like the one shown below. The scientist knows that trilobites only lived during the Paleozoic era.




The scientist finds fossilized remains of other species in the same rock layer as the trilobite. What can the scientist conclude about these other fossils?

A.

The organisms that formed them must have evolved from trilobites.

B.

The organisms that formed them are still living in modern times.

C.

The organisms that formed them likely also lived during the Paleozoic era.

D.

The organisms that formed them likely went extinct before the Paleozoic era.

Answers

Answer:

C.  The organisms that formed them likely also lived during the Paleozoic era.

Explanation:

The organism which was found by the scientist in the same rock layer indicates that the organism was present in the same Paleozoic era where trilobites were present because both organisms found in the same rock layer. Paleozoic era was present 542 million years ago and ended into 251 million years ago. At that time, great change occurs on the Earth. In this era, plants were spreading across the earth surface and first vertebrates species start colonized.

What is a visual model used to measure the average amount of dietary intake of a black bear.

Answers

Answer:

Shaggy black fur and large size distinguish this animal from all other Adirondack mammals. The black bear has a short, inconspicuous tail. The body and legs are short. The head is broad with erect, rounded ears. The muzzle, grizzled with brown, is long and narrow. The eyes are small and dark. Both front and hind feet have five toes, each with a large claw. A white blaze is often present on the throat or chest. Color phases occur but are uncommon in eastern populations. Adults are approximately 50 -78 inches in length. Average-sized adult males weigh 300 lbs. and females 150 lbs.

Explanation:

Which organism fix nitrogen in the soil ?

Answers

Answer:

Diazotrophs

Explanation:

The nitrogen-fixing bacteria and other micro organisms that fix nitrogen are collectively called 'Diazotrophs'. There are many strains of these bacteria in soil, which perform this function. They are important agents in the 'Nitrogen Cycle'.

There are two main groups of organisms that fix nitrogen in the soil: diazotrophs and cyanobacteria.

What are these organisms?

Diazotrophics are bacteria that can convert atmospheric nitrogen into ammonia, which is a form of nitrogen that plants can use. Some examples of diazotrophs include Rhizobium, which is found in the root nodules of legumes, and Azotobacter, which is a free-living bacterium.

Cyanobacteria are photosynthetic bacteria that can also fix nitrogen. They are found in aquatic and terrestrial environments, and they can play an important role in the nitrogen cycle.

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9x - 14y + 9 + 13y​

Answers

Answer:

9x−y+9

Explanation:

I used a calculator

Answer:

Explanation:

First you must put all the like terms together. So 9 can be at the front since it is the only one that is without a letter. The you must put the ys and xs together. It should now look a bit like 9-14y+13y+9x. I think you are able to work it out from there. If not please contact me again

i’ll mark you brainliest plssss helpppp!

Answers

Answer:

I think it would be some stones are harder or it maybe just it has just crystallized.

Explanation:

Answer:

Quartz is the hardest mineral of the minerals on the table.

Explanation:

Since a scratch was produced with Chalcopyrite, Pyrite and Orthoclase but not on Quartz, clearly Quartz was the hardest mineral of the minerals that were tested.  To prove this, we can search up the hardness of each mineral.  Quartz on the hardness scale is 7, Orthoclase on the scale is 6, Pyrite is 6 and Chalcopyrite is 4.  Quartz being the highest at 7 is the hardest of the minerals

The hypothalamus controls excretions from the pituitary gland. It causes the release of some hormones from the pituitary by direct nervous control, and others by secreting releasing and inhibiting factors that stimulate the pituitary to secrete hormones. Which best describes the hypothalamus?

Answers

Answer:

It causes the release of some hormones from the pituitary,,,,

Although we rely on coal, oil, and natural gas to produce energy, some environmental scientists have proposed that we use less fossil fuel. One reason to support this proposal is to

Answers

Where is the question

Answer:

B

Explanation:

how is martinluther?

Answers

Answer:

Explanation:

dead... he is an iconic african-american who was a civil rights leader and gave a speech ¨I had a dream" in Washington D.C.

Answer:

who is martin luther a german professor

Explanation:

martin was a German professor of theology, composer, priest, Augustinian monk, and a seminal figure in the Protestant Reformation.

What occurs during G1 and G2 in the cell cycle?
A) synthesis of the cell
B) growth of DNA
C) growth of the cell

Answers

Answer:

growth of the cell

Explanation:

Answer:

C. growth of the cell

Explanation:

The G stands for growth of the cell.

Interphase is composed of G1 phase (growth of the cell), followed by S phase (DNA synthesis), followed by G2 phase (growth of the cell).

Seif’s pet mouse had babies. Five of the babies were black and two were white. The father mouse was black. The mother mouse was white. Seif and his friends wondered why the mice were different colors.

These were their ideas:

Jerome: Baby mice inherit more traits from their fathers than their mothers.

Alexa: Te baby mice got half their traits from their father and half from their mother.

June: Male traits are stronger than female traits.

Seif: Black mice have more traits than white mice.

Fiona: The black baby mice are probably male and the white baby mice are probably female.

Lydia: Parent’s traits like fur color don’t matter—nature decides what something will look like.

Billy: Blood type determines what traits babies will have.

Which friend do you most agree with and why? Explain your thinking.

Answers

Answer:

Lydia

Explanation:

The black fur in mice is probably more the dominant-inherited trait. The white fur is probably a recessive gene so less of the baby mice will have white fur.

The characters of offspring is decided by their traits, which they get from their father and mother. Sefi's pet mice have different colors because it got half traits from mother and half from father.

What is genetic inheritance?

Genetic inheritance means transfer of characters from parents to offspring. Due to this reason, offspring have characters from both father and mother.

This process is based on Mendalian principle. Any character of offspring is determined by both father and mother, not from the single one.

Here, as the father mouse was black and the mother mouse was white, so according to Mendalian principal, there were difference in the colors of baby mice.

Thus, one should be agree with Alexa, who gave an idea that the baby mice got half their traits from their father and half from their mother.

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Fill in the blanks with the correct word or phrase

The process of change in a population overtime is called --------------

The process by which organisms that are better adapted to the environment survive and reproduce is called --------------------

Answers

Answer: Microevolution.

Explanation: Microevolution is the change in allele frequencies that occurs over time within a population..

When is lactic acid produced

Answers

During intense exercise, there may not be enough oxygen available to complete the process, so a substance called lactate is made. Your body can convert this lactate to energy without using oxygen. But this lactate or lactic acid can build up in your bloodstream faster than you can burn it.

hope this helps :)

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A scientist finds a snail fossil in a layer of rock in a desert which action would help the scientist determine what the environmental conditions may have been when the snail existed?

Answers

Answer:

you can suppose from that statement alone that the snail lived in. climate that was humid, wet and full of life in the past. you can also see the stratus of rock layers fo determine how long it could have been, and use carbon dating as a sure way to get the dating and time period correctly.

What crops are typically genetically modified?

Answers

More than 90% of all soybean cotton and corn acreage in the U.S. is used to grow genetically engineered crops. Other popular and approved food crops include sugar beets, alfalfa, canola, papaya and summer squash. More recently, apples that don't brown and bruise-free potatoes were also approved by the FDA.

Can someone help me in biology really quick because I need the help ASAP

Answers

Answer:

wheres the question?

Explanation:

Answer: Type A

Explanation:

Maintaining fish populations preserves the ocean as a resource and is
fundamental to protecting biodiversity."

Answers

Answer: True

Explanation:

The marine environment is associated with both biodiversity health and quality of water. The overfishing will not only harm the desired aquatic species but can also kill undesirable fishes and plant species which are removed from their habitat during the fishing process. The some fish species eat up the pollutants in water and sea debris making the environment clean and healthy for other aquatic species. Thus maintaining the fish population will preserve the aquatic environment.

When you have me you feel like sharing me but if you do share me you don’t have me what am I

Answers

Answer:

A secret. When you have a secret, you want to tell other people, but when you do, it's no longer a secret.

Answer:

A secret

Explanation:

When you have a secret, you want to tell other people, but when you do, it's no longer a secret. Hope this helped :)

Please help me with 4 through 6

Answers

4.b 5.c 6.c is the right answer

What reasoning correctly pairs a body system with its relationship to the digestive system?

Answers

Answer:

Respiratory system.

Explanation:

This is the system which shows a good pairing with the digestive system. This is because for energy to be synthesized by oxidative phosphorylation,  respiratory systems need  the supply of  Oxygen. The oxygen is needed to accept the final electron in the synthesis of ATPs during oxidative phosphorylation.

This process begins with substrate level phosphorylation-glycolysis- the breaking down of glucose from the digestive system.

Hence, respiratory system needs  the  supply of oxygen for the metabolisms of glucose obtained from digestive system to generate energy( extract the energy in glucose) for the body.


Which kingdom is composed of multicellular autotrophs?


Answers

Answer:

Plantae kingdom

Explanation:

Plantae kingdom

Plants. The Plant or Plantae kingdom encompasses all flowering plants, mosses and ferns. Plants are multi-celled, complex organisms and are considered Autotrophic.

The kingdom would be plant

Which type of connective tissue has the most fibers?

A) Loose connective tissue
B) Fibrous connective tissue
C) Cartilage
D) Bone

Answers

Answer:

a loose connective tissues

Explaanation:

Option B) Fibrous connective tissue is a  type of connective tissue that has the most fibers among all.

Connective tissue is a tissue that not just supports, and protects, but also provides structure to other tissues and organs of an organism's body.  There are four classes of connective tissues:

Loose connective tissue - contains few fibers with parts of ground substances Fibrous connective tissue - has the most abundant fibers. Cartilage - matrix has a large number of fibers with few elastic and collagen fibers. Bone - some amount of fibers present in the matrix of the bones.

Fibrous connective tissue or dense connective tissues has most fibers as it is made up of bundles of collagen fibers present parallel. These are present in tendons, ligaments, and dermis.

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Many people believe that diet soda is healthier than regular soda because it contains less sugar and fewer calories. However, several scientific studies have found that diet soda may be associated with diabetes, weight gain, and heart disease. In fact, those who drink one diet soda per day have an increased risk of heart attack and stroke compared to those who do not drink diet soda each day or at all. What claim is being made? Diet soda is healthier than regular soda. Diet soda tastes better than regular soda. Diet soda may increase the risk of disease. Diet soda may contain more calories than regular soda.

Answers

Answer:

C i got it right;)

Explanation:

Do not drink diet soda each day or at all. What claim is being made Because, Diet soda may increase the risk of disease.

Is diet soda healthier than regular soda?

Despite it being a lower or zero calorie drink , it may still theraise  risk of situation such as diabetes and obesity.

Diet soda shows no fitness uses other than serving as a tool that people can use to wean themselves off regular soda.

Thus, option "C" is correct,  Diet soda may increase the risk of disease.

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Which two activities provide the reactants for cellular respiration

Answers

Answer:

The Reactants

Explanation:

Oxygen and Glucose are both reactants of cellular respiration.Oxygen enters the body when an organism breathes.Glucose enters the body when an organism eats.

Answer:

D-breathing in air and eating food

Explanation:

on edge plz give me brainly

Plan an investigation to determine which part of the oregano plant has the highest concentration of chemicals with antimicrobial properties

Answers

Answer:

The study reported here presents a comparative screening of three medicinal plants including oregano (Origanum vulgare L.), lavender (Lavandula angustifolia) and lemon balm (Melissa officinalis) having the same geographical origin, the Southeast region of Romania, and growing in the same natural conditions. The contents of total phenolics and total flavonoids for the extracts of these were determined. Furthermore, the total antioxidant capacity was also evaluated. It was found that Origanum vulgare and Melissa officinalis extracts present the most effective antioxidant capacity in scavenging DPPH radicals, while Lavandula angustifolia is less active. High performance liquid chromatography-mass spectrometry analysis was used to identify the components of extracts. Major phenolic acids identified in the analysed species were ferulic, rosmarinic, p-coumaric and caffeic, while predominant flavonoids were quercetin, apigenin kaempherol, which were present as glucosides.

Explanation:

ecology succession biology succession ​

Answers

Answer:

There are two types of succession.

Primary Succession - A bare mass of rock developing an ecosystem from scratch. It will use lichens and moss to create soil... a good example of this is a new land mass formed by a volcano.

Secondary Succession - A environment and ecosystem that was destroyed but does not need to start from scratch. It just needs the plants to regrow and attract wildlife back again... a good example of this is a forest destroyed by a wildfire.  

Explanation:

Describe the shape of the earths orbit around the sun

Answers

Earth's orbit is not a perfect circle. It is elliptical, or slightly oval-shaped. This means there is one point in the orbit where Earth is closest to the Sun, and another where Earth is farthest from the Sun.

pls mark pls pls pls

the process of retting softens the tissue and helps to separate the fibres true or false

Answers

Answer:

True

Explanation:

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Answer: True

Explanation:

the process of retting softens the tissue and helps to separate the fibres.

the earths crust is
1. heavier
2.softer
3.thicker
4.thinner

Answers

Answer:

The Earth's crust is thicker

What benefit did the invasive species called Japanese white eye bring to the ie ie vine?

Answers

Answer:

Pollination of plants

Explanation:

This species of birds is thought to help in preserving and conserving the native plant species of Hawaii by helping in dispersing their seeds. They are the main pollinators of the ieie vine. These was a role that was once performed by the long extinct Hawaii native bird species (they became extinct due to the invasion of these Japanese white eye).

Answer: It helped in pollination.

Explanation: edmentum/plato

The invasive species called Japanese white eye was beneficial to native vine pollination.

What does respiration mean in biological terms?​

Answers

Answer: Respiration is the biochemical process in which the cells of an organism obtain energy by combining oxygen and glucose, resulting in the release of carbon dioxide, water, and ATP (the currency of energy in cells).

Explanation:

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