You are inserting your gene of interest into the Lac Z gene in a plasmid also containing a tetracycline resistant gene. You plate your transformed bacteria on media containing tetracyline and X-gal (the LacZ substrate that can form a blue dye). What results would indicate a clone with recombinant plasmids?

Answers

Answer 1

Answer:

A white colony in tetracyline plates

Explanation:

Beta-galactosidase (also known as beta-gal or β-gal), will not be produced if a gene was cloned into the Lac Z gene. It is because β-galactosidase is produced by the activation of the lacZ gene, and thereby β-gal can not be produced in the absence of this lac operon. In consequence, X-gal will not be hydrolyzed by the β-gal enzyme in a reaction that releases a blue pigment, and thus the colony will be white. In gene cloning, X-gal enables to determine whether a cell is expressing a functional β-gal enzyme, which is known as the "blue/white screening method".


Related Questions

Identify which statement describes a scientific law

Answers

Answer:

Newton's three laws of motion are examples of laws in physical science. A scientific law states what always happens but not why it happens. Scientific theories answer “why” questions.

Explanation:

HELP ME PLEASE PLEASE HELP IM BEGGING YOU​

Answers

Answer:

C

Explanation:

The hot air and cold air mix making it updraft and downdraft

Where in the cell does the process of cellular respiration take place?

Answers

Answer:

It begins in the cytoplasms of the cells ! It then moves to the mitochondria

What two factors cause natural gas to form?​

Answers

Answer:

♡ good morning! ♡

the answer is thermogenic methane

and the combination of compression

and high temperature. have a great day! ☆

- madeline/madi

✧・゚: *✧・゚:・゚✧*:・゚✧・゚: *✧・゚:・゚✧*:・゚✧・゚

Which of these is a possible description of the offspring? some flowers with white petals and some with light blue petals some flowers with dark blue petals and some with white petals all flowers with light blue petals all flowers with some dark blue petals and some white petals

Answers

Answer:

All flowers with some dark blue petals and white petals.

Explanation:

Answer:

ANSWER CHOICE IS D

Explanation:

correct on the edg test

An ecosystem is best described as the

A) Type of food that an organism eats
B) Type of home an organism builds
C) Group of organisms in a particular place
D) Living and nonliving factors in one place

Answers

I think it’s C) Group of organisms in a particular place

For psychologists, controlling electronic information is important because electronic information is __________.

Answers

B.
trivially easy to copy and redistribute

Answer:  B trivially easy to copy and redistribute

Explanation:

If the last pair reflects whether the organism is a male or female, which would the organism be?

Answers

Complete question:

There is a karyotype in this question, which is missing. You will find it in the attached files.

Answer:

The individual is a male.

Explanation:

In mammals, sex is determined by a pair of sexual chromosomes. Sexual chromosomes in humans and other mammals are X and Y. The females are represented by the combination of two X chromosomes, while males are represented by the combination of an X chromosome with a Y chromosome. This is, females are XX, and males are XY. When looking at the sexual pair of chromosomes in a karyotype, you can figure out which is the individual´s gender by comparing their sizes. The X chromosome is much bigger and longer than the Y chromosome.

In the shown karyotype, the last pair represents the sexual chromosomes. You can see that one of them (the one on the left) is long and express a sort of J shape, while the other chromosome (on the right) is very short and small. According to what we previously mentioned, the X chromosome is the long one on the left, and the Y chromosome is the short one on the right. This is a male individual.

Sex chromosomes are the central determinant that defines the sex of an individual. By analyzing the karyotype one can conclude the sex chromosomes and the autosomes.

The correct answer is:

The individual is male.

Reason for the answer:

In organisms like mammals, the sex of an individual is determined by sex chromosomes. Humans and many other mammals have two sex chromosomes X and Y.

The females have two X chromosomes(XX), while males have one X and one Y chromosome (XY).

By studying karyotype one can distinguish among the X and Y chromosomes. The X chromosome is bigger than the Y chromosome.

The karyotype provided in the question represents sexual chromosomes. The chromosome present on the left is large and is J-shaped which represents the X chromosome, while the chromosome present on the right is small and depicts the Y chromosome.

Therefore, the given Chromosome is the Y chromosome and represents a male chromosome.

To learn more about sex chromosome follow the given link:

https://brainly.com/question/19426394

Why does the nucleolus disappear during prometaphase?

Answers

Answer:

Chromosomes

Explanation:

The Chromosomes start to separate during this process and the nucleolus is taken out before metaphase so the chromosomes can take the place of the nucleus

Plz mark brainliest

Can a bearded dragons eat a Hammerhead worm.

Answers

Answer:

Eww what is that in the first place. But if that thing is not posinond i think people and dragons can eat it.

Explanation:

Answer:

no it looks to big for a bearded dragon even an adult

Explanation:

________________ is a characteristic that can be used to place an organism in a particular kingdom.
a. growth
b. cell type
c. health
d. life span

Answers

Answer:

b...which would be cell type:)

Explanation:

Answer:

It would be B. cell type        

In '57 Chevy's, the gene for having fuzzy dice (F) on the rear view mirror is dominant
over the gene for having smooth dice (f) on the rear view mirror. The genotype of a
smooth dice is -
FF
heterozygous
ff
cannot be determined

Answers

Answer:

ez i just took the test is FF pls give brainly

Explanation:

According to the scenario, the genotype of a smooth dice is ff. Thus, the correct option for this question is C.

What is a Genotype?

A genotype may be defined as the ultimate combinations of alleles of those genes which are significantly taken for specific studies in order to determine some facts and knowledge.

According to the context of this question, it is clearly given that the gene for having fuzzy dice (F) on the rearview mirror is dominant over the gene for having smooth dice (f) on the rearview mirror. This means that F>f. So, it the phenotype of a recessive allele has to visualize in any organism, its genotype must carry form forms of the recessive allele.

Therefore, according to the scenario, the genotype of a smooth dice is ff. Thus, the correct option for this question is C.

To learn more about Genotypes and phenotypes, refer to the link:

https://brainly.com/question/902712

#SPJ2

If the haploid number of an organism is 32, how many pairs of chromosomes would be found in each of the cells of the adult organism?

1. 128
2. 16
3. 32
4. 64

Answers

64 is the answer because you would double 32 hope it helps.

- Pain, swelling, or redness where the shot was given
- Mild fever
- Chills
Feeling tired
Headache
- Muscle and joint aches"
.
A lot of us can probably state from experience feeling at least a few of these symptoms with
vaccines. I just got my flu shot on Sunday, and I personally experienced pain at the site of the shot,
tiredness, and muscle and joint aches. (These symptoms were mild and completely worth reducing
the risk of contracting and spreading influenza this fall and winter.)
What's really cool is these symptoms actually serve as a sign that our immune system is at work.
Explain how these mild symptoms following a vaccine indicate our immune system is working
properly. Although we often think of how vaccines affect the third line of defense through the
creation of memory cells and production of antibodies, consider what you learned in the previous
chapter on the second line of defense and how this relates to the mild symptoms following
vaccination.

Answers

These symptoms show that the vaccine is taking action in your body and is preparing your body to fight any king of flu or cold like virus. Hope this helps

How do the nutrients in the food you eat reach the cells in your body?​

Answers

Facillitated Diffusion

Question 2 (5 points)
Climate is the AVERAGE yearly conditions of temperature, precipitation, winds, and clouds in an area. Obj 6
O True
False

Answers

The answer would be true (:

Photosynthesis occurs both in plants and animals true or false

Answers

Answer:

false!

Explanation:

it only occurs in plants <3

If humans were exposed to ultraviolet rays and high levels of radiation, it could lead to greater chances of mutations occurring in the genes of their cells. This could then lead to the cause of what

Answers

Answer:

Cancer

Explanation:

sometimes a mutation of the cells leads to cancer because the cells cant stop reproducing, so you have cells that choke each other out pretty much

Which event occurs during meiosis that increases genetic variation and contributes to the process of
evolution?
O A. Cytokinesis occurs and separates the cytoplasm and its contents into daughter cells.
O B. Diploid gametes divide to form haploid daughter cells with unique genetic makeup.
O C. During interphase, homologous chromosomes are duplicated to form sister chromatids.
D. Segments of DNA are exchanged between homologous chromosomes during crossing over.

Answers

D
When the DNA is exchanged, it increases the amount of variation.

Answer:

D. Segments of DNA are exchanged between homologous chromosomes during crossing over.

Explanation:

This ensures that the chromosomes get all mixed up in order to make new genes.

8 facts about the Geosphere

Answers

1 geosphere is the earth's solid rock, 2Without the geosphere it wouldn’t be possible for humans to live on any planet because there would be no solid hard ground.
3The geosphere is 4.6 billion years old.
4Piling up our waste in landfills affects the geosphere. Yeah I know we are terrible...
5 the geosphere includes rocks and minerals
6 with no geosphere there wouldn’t be lakes rivers etc...
7 the geosphere is with a radius of 6,357 kilometers
8 we all need the geosphere to live
Hope this helped!!!

Facts:

The Geosphere is basically the insides of the earth so breaking it up into eight parts will be based on each section of it.

1. The crust is the top layer of the Earth, it consists of the water and land we see. It is a shell that lays atop or covers the Upper mantle piece.

2. The mantle is a layer between the crust and core, Earth's mantle is a rocky shell that is roughly 2,900km thick and is 84% of Earth's volume.

3. The Geosphere is the solid part of the Earth that consists of rock, soils and sediments on the surface of the Earth.

4. The core is the innermost layer of the Earth and is up to 6371km from the surface of the Earth, it consists of Nickel and Iron and are seen as main components of it.

5. The outside of the core is known as the molten core due to it mainly being liquid metal from the heat and the inner portion of the core known as the solid core.

6. Another name or term given to the Geosphere is called the lithosphere, depending on the definition they both mean the same thing but are still argued by scientists.

7. The Geosphere is in constant motion due to the tectonic plates. These plates are what causes earthquakes ,volcanoes to erupt and even tsunamis.

8.There are eight major tectonic plates making up the earth's geosphere. They are constantly moving, but usually only a few centimeters each year, this is known as continental drift.

Many people die whilst they are waiting for an organ transplant. Why is this?

Answers

Answer:

because the organs they need are not donated in time!!!

Describe the osmotic regulation strategies of marine fish

Answers

Answer:Water will diffuse into the fish, so it excretes a very hypotonic (dilute) urine to expel all the excess water. A marine fish has an internal osmotic concentration lower than that of the surrounding seawater, so it tends to lose water and gain salt. It actively excretes salt out from the gills.

Explanation:

Winds curve as they blow due to

Answers

Answer:

lack of pressure difference between regions

Answer:

C

Explanation:

Wins curve due to the lack of pressure differences between regions

How will agents of weathering such as wind and water most likely affect this landscape

Answers

Answer:

Answer

Explanation:

The wind and water can break down the landscape and affect its physical formation. Water for example can break down rocks and make a ditch from 1 foot deep to 6 feet deep.

What can a fertilized egg grow into?
A. a heart-shaped plant
B. a mature fern

Answers

A Mature fern. Hope this helps

An essay about clubfoot

Answers

Answer:

use this- Clubfoot is a deformity in which an infant's foot is turned inward, often so severely that the bottom of the foot faces sideways or even upward. Approximately one infant in every 1,000 live births will have clubfoot, making it one of the more common congenital (present at birth) foot deformities.

Explanation:

What is the function of the testes? There is more than one answer.

A. Make estrogen

B. houses the developing fetus

C. houses the developing sperm

D. make testosterone

E. is the site of oogenesis

F. the site of spermatogenesis

Answers

Answer:

D

Explanation:

function of teses is to make testosterone

C, D, and F... hope that helps!

Section 2: Conduct internet research on your selected ecosystem to help you generate a list of three criteria and two constraints. Your criteria and constraints should consider relevant factors to the problem, such as costs, reliability, safety (to humans and wildlife), human needs, environmental impact, local biodiversity, and the aesthetics of the area. List the criteria in order of most to least important and assign each to a related sub-problem. In the rationale column, explain why you placed your criteria in the order you selected.

Answers

Answer:

Constraints:

Funding

Protective equipment

Research

Installation

Criteria

Fertilizers

Rationale:

This is a big deal being that fertilizers are needed to help plants experts are still looking for ways to knock down the amount used.

Which Sub-Problems does this criteria address?

The main environmental problem associated with fertilizer use is contamination of water with nitrates and phosphates. The nitrogen from fertilizers and manures are eventually converted by bacteria in the soil to nitrates. ... High nitrate levels in drinking water are considered to be dangerous to human health.

Criteria:

Human Waste

Rationale:

Human waste is a large problem and the government is trying to stop the public from disposing of it.

Which Sub-Problems does this criteria address?:

Globally, almost 80 percent of the world's wastewater flows back into the ecosystem without proper treatment. This means that almost a quarter of people drink water contaminated with human waste, according to United Nations Water. That incubates deadly diseases, including cholera, dysentery, typhoid and polio.

Criteria:

Oil  

Rationale:

Oil is needed for just about everything but it is very costly when it comes to the environment.

Which Sub-Problems does this criteria address?

Oil spills have a devastating effect on the environment, ruining habitats and killing the organisms that live there by sticking to them, destroying their food sources and poisoning them. Additionally, oil hurts the economy by harming the fishing industry, as well as other trades that rely on the ocean.

Explanation:

What evolutionary advantage might on/off signaling have over this continuously variable kind of signaling?

Answers

Answer:

Hello the options to your question is missing below are the missing options

A threshold to cause a signal is lower than in graded potentials. Graded potentials can cause a strong but single depolarization signal.

The speed of propagation is much faster as depolarization in one location causes depolarization in the neighboring ones.

Propagation of the on/off signals requires less ATPATP because the neighboring locations can depolarize each other.

The on/off signal may be propagated over long distances without any decay. Therefore, the signal is reliable.

The power of the stimulus does not matter once it triggers the signal. Therefore, different stimuli may cause the desired response.

Answer :The speed of propagation is much faster as depolarization in one location causes depolarization in the neighboring ones.

Propagation of the on/off signals requires less ATPATP because the neighboring locations can depolarize each other.

Explanation:

Some of the evolutionary advantage that on/off signaling have over this continuously variable kind of signaling are :

The speed of propagation is much faster as depolarization in one location causes depolarization in the neighboring ones.Propagation of the on/off signals requires less ATPATP because the neighboring locations can depolarize each other.

why are there usually several types of pigments present in cloroplast

Answers

Answer:

Because the pigments interact with different light to absorb different light wavelengths.

Other Questions
en una caja hay 8 canicas cinco rojas y tres verdes y la Bola que se retira no se reemplaza determina Cul es la probabilidad de sacar dos bolas verdes What is a sexual repuduction? 9. A contestant on a game show must guess the price of a new car. The contestant willwin if his guess is within $1000 of the price of the car. If the price of the car is$24,995 and the contestant's guess is represented by g, what absolute valueinequality represents this situation? give a direction to your friend at cape coast to locate your newly built house. which is a true statement about ribosomes?ribosomes are found on both smooth and rough endoplastic reticulumO ribosomes are made in the cell membraneribosomes build proteinsribosomes are surrounded by a membrane Suppose that Rosie and Ross are both carriers for PKU (a rare autosomal recessive disease.) If they have eight children, what is the probability that all of eight children will have the same phenotype? HELP!!! 50 POINTS!! I WILL REPORT IMPROPER ANSWERS!!! 10 points plus brainliest please help due in 20 minutes!!! Why wasOperationOverlord (D-Day)so important? ill gives brainiest The average height of an ostrich is 255 cm.Suppose that you want to make a scaledrawing of an ostrich. The scale is 30 :1.What height will you make the ostrich inyour drawing? Explain your thinking. on what grounds did the Illinois supreme court decide to allow the Nazi rally? Need help ASAPsolve the equation: 3(2x+5)= 3What does x equal- x=2- x= -2- x=6 which one is the the oldest and youngest? Economics..Match the following.. Solve 3x + 8 = x + 16 The Cask of Amontillado (Fortunatos point of view.) how did the land ordinance provide an organized development of the new land in the northwest territory? To communicate an action will occur in the future, the sign moves forward, away from the body, starting Sol purchased land as an investment on February 12, 2018 for $85,000. On January 31, 2019, Sol sold the land for $90,000 cash. What is the nature of the gain or loss? Write 4(2z+5) in expanded form