why are chromogenic assays (as opposed to direct measurements at the wavelength which proteins naturally absorb light) used in the lab to measure protein concentrations?

Answers

Answer 1

Chromogenic assays are used in the lab to measure protein concentrations because they are more sensitive, specific, and convenient than direct measurements at the wavelength at which proteins naturally absorb light.

In direct measurements, it can be difficult to distinguish between different proteins that may have overlapping spectra. On the other hand, chromogenic assays involve adding a specific substrate to the protein sample and measuring the resulting color change, which can be used to quantify the amount of protein present. This method is more sensitive and specific as it allows for better discrimination between different proteins and reduces background interference.

In addition, chromogenic assays can be performed in a quicker and more automated manner than direct measurements, making them a convenient choice for many laboratories. By using chromogenic assays, the laboratory can efficiently and accurately measure protein concentrations in a range of sample types, including blood, plasma, and tissue samples.

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Related Questions

Photosynthesis converts the ______ energy in sunlight into the ______ energy in sugars.

Answers

Photosynthesis converts the solar energy in sunlight into chemical energy in sugars.

Photosynthesis is the process by which plаnts use sunlight, wаter, аnd cаrbon dioxide to creаte oxygen аnd energy in the form of sugаr. Most life on Eаrth depends on photosynthesis. The process is cаrried out by plаnts, аlgаe, аnd some types of bаcteriа, which cаpture energy from sunlight to produce oxygen ([tex]O_{2}[/tex]) аnd chemicаl energy stored in glucose (а sugаr). Herbivores then obtаin this energy by eаting plаnts, аnd cаrnivores obtаin it by eаting herbivores.

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how do scientists confirm that a cell has incorporated recombinant dna? sequence the genome of many cells screen for the expression of a reporter gene perform a western blot grow cells in the presence of an antibiotic perform polymerase chain reaction and run a gel

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The scientists confirm that a cell has incorporated recombinant DNA by following ways: (b) screen for the expression of a reporter gene; (d) perform polymerase chain reaction and run a gel; and (c)  grow cells in the presence of an antibiotic.

Recombinant DNA is the piece of DNA generated by the combination of genes from two or more species. This technique is a gene manipulation technique where the genes of interest can be obtained and inserted into any species to obtain the desired results.

Polymerase Chain Reaction (PCR) is a technique where small fragments of DNA can be used to produce its millions of copies within a small time period.

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when might negative feedback fail to regulate hormone production, and how would this affect hormone secretion?

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Negative feedback fail to regulate hormone production can be production of hyposecretion that could be caused by an endocrine gland pathology that prevents negative feedback from working.

What do we mean by hormones?

One of the many substances produced by the body's glands. Hormones, which circulate in the bloodstream, regulate the actions of specific cells or organs. Some hormones can also be manufactured in a laboratory. Hormones, the body's chemical messengers, communicate with tissues and the bloodstream. Hormones have an impact on a wide range of bodily functions, including growth and development, metabolism (the process by which your body converts food into energy), other function, reproduction, and mood. Hormones influence blood sugar, blood pressure, growth and fertility, metabolism, and even sleep. They have a significant influence on how we think and behave on a daily basis. Hormones are undeniably potent.

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a small, circumscribed lesion, usually less than 1 cm in diameter, that is elevated or protrudes above the surface of normal surrounding tissue is termed a

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Plaques are tiny, confined lesions with a diameter of less than 1 cm that really are elevated and protrude beyond the normal surrounding tissue's surface.

A lesion might be dangerous.

Skin lesions are common and can develop as a result of an accident and other skin damage, like a sunburn. In some circumstances, infections or autoimmune conditions may be an indication of deeper issues. The vast majority of lesions were benign (noncancerous) or safe, but they may potentially be signs of a more serious problem.

Does a lesion require surgery?

Skin growths are removed from your skin via a procedure or treatment called skin lesion removal. It could be necessary to remove a skin lesion if it is excessively large, nasty, or bothersome. If a lesion turns to be cancerous or precancerous, surgery may be required to remove it. Simple skin lesions can usually be treated at a routine doctor's appointment.

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in a cross of a pprr with a pprr, what proportion of offspring would have white kernells? group of answer choices 25% 100% 50% 75% 12.5%

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In a cross of two Pprr organisms, the offspring will all be heterozygous (PpRr). This means that the offspring will have a 50% chance of having white kernels and a 50% chance of having yellow kernels. Therefore, the proportion of offspring with white kernels will be 50%.

White kernels are a type of seed that is produced by certain plants. They are typically smaller and rounder than their yellow counterparts. The white color is caused by the lack of carotenoids in the seed, which are responsible for the yellow color. White kernels are usually used for milling and baking, as they are more flavorful and have a better texture than yellow kernels. White kernels are also more resistant to pests and diseases, making them a popular choice for farmers.

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Why does origin want to clone meaty dark feathered birds

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The origin wants to clone dark feathered birds as dark fathers were more efficient in providing heat and effective fly to the birds.

According to the researchers, darker feathers heated up more quickly than lighter ones and got hotter as well. However, the birds that truly benefited were those with light-colored or white wing feathers close to their bodies and dark or black wing feathers on the remainder of their wings. Therefore, the original intended to clone these birds with dark feathers.

Moreover, the researchers discovered that the temperature of black and white feathers on the same wings varied by as much as nine degrees, which was sufficient to induce a convection current in the air just above the wing that moved from the bird's body outward along the wing. They saw that this increased airflow, which they reasoned increased flying effectiveness.

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9.

What does it mean for an organism to have high fitness?

A.

Organisms with high fitness in an environment can survive and reproduce.

B.

Organisms with high fitness can survive in any environment.

C.

Organisms with high fitness do not produce many offspring.

D. Organisms with high fitness developed beneficial traits during their lifetime.

Answers

An organism with high fitness in a particular environment can survive as well as reproduce.

The correct option is option A.  

Natural selection is very important in evolution. According to natural selection, only the organisms which have those characteristics which can help them thrive in a particular environment survive whereas the ones which do not have cannot survive and reproduce.

An organism which has high fitness is going to have adaptations that are well suited to their particular environment allowing them survive as well as reproduce. An organism that has low fitness has characteristics which are not well suited to their particular environment meaning they almost will certainly die without reproducing.

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why is the human effective population size so much smaller than the human census population? group of answer choices there is non-random mating among humans not everybody contributes equally to next generation human populations went through bottlenecks all of the above none of the above

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"All of the above" (Option E) are reasons why the human effective population size is smaller than the human census population.

The effective population size is a measure of the number of individuals in a population who contribute offspring to the next generation, while the census population is a count of all individuals in a population, regardless of their reproductive contribution. Non-random mating, unequal contributions to the next generation, and past population bottlenecks can all reduce the effective population size compared to the census population. For example, if a small subset of individuals within a population contributes disproportionately to the next generation, the effective population size would be much smaller than the census population. Similarly, if a population went through a bottleneck event in the past, the effective population size would be smaller as well.

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What are lichens a symbiotic association of?

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Lichens are commonly recognized as a symbiotic association of a fungus and a chlorophyll containing partner, either green algae or cyanobacteria, or both.

Chromosomes tightly coiled structure enables them to be:_________

Answers

Chromosomes tightly coiled structure enables them to be segregated during cell division.

A chromosome is a long DNA molecule with part or all of the hereditary material of an organic entity. In many chromosomes the extremely lengthy meager DNA segments are covered with bundling proteins; in eukaryotic cells the most significant of these proteins are the histones. These proteins, helped by chaperone proteins, tie to and consolidate the DNA particle to keep up with its integrity. These chromosomes show a mind boggling three-layered structure, which assumes a huge part in transcriptional regulation.

Chromosomes are typically noticeable under a light magnifying lens just during the metaphase of cell division (where all chromosomes are adjusted in the focal point of the phone in their consolidated form).[4] Before this occurs, every chromosome is copied (S stage), and the two duplicates are joined by a centromere, coming about either in a X-molded structure (presented above), in the event that the centromere is found equatorially, or a two-arm structure, assuming the centromere is found distally.

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Match the pollutant to the water pollution classification

Top
1. Heat
2. Parasitic worm
3. Animal feces
4. Household cleaners (drain-O and bleach)
5. Light fluid
6. Arsenic

A. Inorganic chemical
B. Physical agent
C. Heavy metal
D. Organic matter
E. Organic chemical
F. Pathogen.

(Example on how to answer=
1. Baby belongs to, F. Couch.).

Answers

ater pollutants can be classified as organic pollutants, inorganic pollutants, pathogens, suspended solids, nutrients and agriculture pollutants, thermal, radioactive, and other pollutants.

Answer:

1- Heat -- B. Physical agent

2- Parasitic worm -- F. Pathogen

3- Animal feces -- D. Organic matter

4- Household cleaners (drain-O and bleach) -- E. Organic chemical

5- Light fluid -- D. Organic matter

6- Arsenic -- C. Heavy metal

why does ecology need to study the biotic and abiotic elements?(1 point) responses it is studying a species. it is studying a species. it is studying the community. it is studying the community. it is studying a population. it is studying a population. it is studying the ecosystem.

Answers

Ecology needs to study the biotic and abiotic elements because it is studying the ecosystem.

Thus, the correct answer is It is studying the ecosystem (D).

Ecology is а brаnch of science, including humаn science, populаtion, community, ecosystem аnd biosphere. Ecology is the study of orgаnisms, the environment аnd how the orgаnisms interаct with eаch other аnd their environment. It is studied аt vаrious levels, such аs orgаnism, populаtion, community, biosphere аnd ecosystem.

Аn ecologist’s primаry goаl is to improve their understаnding of life processes, аdаptаtions аnd hаbitаts, interаctions аnd biodiversity of orgаnisms. The mаin аim of ecology is to understаnd the distribution of biotic аnd аbiotic fаctors of living things in the environment. The biotic аnd аbiotic fаctors include the living аnd non-living fаctors аnd their interаction with the environment.

Your options aren't well arranged, but most probably your options were

A. it is studying a species.

B. it is studying the community.

C. it is studying a population.

D it is studying the ecosystem.

Thus. D is the correct answer.

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Compare and contrast the characteristics of command and mixed-market economic systems, and give one example of each.

Answers

Answer:Command economies are characterized by large surpluses and shortages, monopolies, and prices set by the government. Mixed economies are characterized by corporate profitability, the use of fiscal and monetary policies to stimulate growth, and the existence of a public and private sector.

Explanation:

calculate the number of possible chromosome combinations that might occur in a gametic cell, given an organism with five pairs of chromosomes.

Answers

The number of possible chromosome combinations in a gametic cell depends on the number of chromosomes and the mechanism of chromosome segregation during meiosis.

In an organism with five pairs of chromosomes, the number of chromosomes in a gametic cell would be half of the number of chromosomes in the organism's diploid cells. That means the gametic cells would have 5 chromosomes.

During meiosis, chromosomes are randomly segregated into the gametic cells, and this process generates unique combinations of chromosomes in each cell. The number of possible combinations of chromosomes in a gametic cell can be calculated using the formula 2^n, where n is the number of chromosomes.

In this case, the number of possible chromosome combinations in a gametic cell would be 2^5, which is equal to 32. Therefore, there are 32 possible combinations of chromosomes that might occur in a gametic cell in an organism with five pairs of chromosomes.

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Name the level of organisation which best describes the interaction of living organisms with the non-living parts of their environment.

Answers

kesonnd

Explanation:

neeee no-good.........

Answer: Ecosystem: is the level organisation best describes the interaction of living organisms with the non-living parts of their environment

Explanation:

An ecosystem consist of all the living things in a particular area together with the abiotic , non-living parts of that environment such as nitrogen in the soil or rain water

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the organelles that digest engulfed bacteria and cell debris are the a. peroxisomes b. endosomes c. lysosomes d. ribosomes e. mitochondria

Answers

The organelles that digest engulfed bacteria and cell debris are the (c) lysosomes.

Lysosomes are organelles that are surrounded by a membrane and contain an assortment of enzymes that are able to degrade all different kinds of biological polymers, specifically proteins. Lysosomes perform the role of the digestive system of the cell.

They are responsible for breaking down the material that the cell has taken in from the outside world as well as for breaking down components that are no longer needed by the cell. Lysosomes contain potent enzymes and acids that enable them to break down and recycle the elements that make up the cell.

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researchers are monitoring the sequence in which blood flows to different regions of theresa's brain while she solves simple arithmetic problems. they are most likely making use of

Answers

Researchers are monitoring the sequence in which blood flows to different regions of Theresa's brain while she solves simple arithmetic problems. They are most likely making use of: fMRI.

fMRI stands for Functional magnetic resonance imaging. It is used to measure the minute changes that occur in the flow of blood due to any brain activity. Thus, solving arithmetic problems is an activity that will cause the blood flow and hence can be detected by this technique.

Brain is the most complex organ of the body that controls different functions of the body. The brain has two hemispheres: the right and the left. The whole brain is divided as: forebrain, midbrain and hindbrain.

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what type of epithelial tissue lines kidney tubules?

Answers

Answer: Simple cuboidal epithelium: This type of epithelium is typically found in glandular (secreting) tissue and kidney tubules.

Explanation:

The numbers of organisms, at each trophic level, in a natural habitat remain more or less constant from year to year. Explain why this is so.

Answers

The number of organisms at each trophic level in a natural habitat will remain more or less constant from year to year if the habitat is in equilibrium.

What is equilibrium?

A habitat is said to be in equilibrium if there are no major changes to the habitat either by natural forces or anthropogenic forces.

A habitat in equilibrium would be a climax habitat. In other words, it would be a habitat that is no longer in succession. It has reached a relatively stable state.

Thus, the number of organisms at each trophic level will remain the same for many years as long as there are no major modifications to the habitat.

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Wastes and now the remains largely of dead organic matter would accumulate, and the nutrients in the waste and dead organisms would not be released back into the environment. The producers would not have had enough nutrients.

Answers

Organic matter, organic material, or natural organic matter refers to the large source of carbon-based compounds found within natural and engineered, terrestrial, and aquatic environments.

What is Organic matter?

It is matter composed of organic compounds that have come from the feces and remains of organisms such as plants and animals. Organic molecules can also be made by chemical reactions that do not involve life.

Basic structures are created from cellulose, tannin, cutin, and lignin, along with other various proteins, lipids, and carbohydrates. Organic matter is very important in the movement of nutrients in the environment and plays a role in water retention on the surface of the planet.

Organic chemicals make up all living things. When organisms are alive, they emit or excrete organic material into the environment, lose body parts like leaves and roots, and their corpses are decomposed by bacterial and fungi after they die.

Therefore, Organic matter, organic material, or natural organic matter refers to the large source of carbon-based compounds found within natural and engineered, terrestrial, and aquatic environments.

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which of the following foods is the best source of insoluble (nonfermentable) fiber? a. kidney beans b. celery c. english muffins made with enriched flour d. corn flakes

Answers

Foods which best source of insoluble(nonfermentable) fiber is a)Broccoli.So,correct option is a.

Dietary fiber is the piece of plant-based food that for the most part goes through your stomach related framework without separating or being processed. There are two kinds of fiber:

Soluble fiberInsoluble fiber.

Soluble fiber breaks down in water, and incorporates plant gelatin and gums. Insoluble fiber doesn't disintegrate in water. It incorporates plant cellulose and hemicellulose.Most plants contain both dissolvable and insoluble fiber, however in various sums. Fiber is a significant piece of a solid eating routine and supports various body frameworks.

As soluble fiber breaks down, it makes a gel that might further develop processing in various ways. Dissolvable fiber might diminish blood cholesterol and sugar. It assists your body with further developing blood glucose control, which can support decreasing your gamble for diabetes.

Insoluble fiber draws in water into your stool, making it gentler and more straightforward to pass with less stress on your entrail. Insoluble fiber can assist with advancing inside wellbeing and consistency. It additionally upholds insulin awareness, and, as solvent fiber, may assist with diminishing your gamble for diabetes.

Hence,correct option is a.

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(Complete question) is:

Which of the following foods is the best source of insoluble (nonfermentable) fiber?

a) Broccoli

b) Kidney beans

c) English muffins made with enriched flour

d) Corn flakes

What do eukaryotic cells have to perform specific functions?

Answers

Different organelles and proteins are needed by eukaryotic cells to carry out various tasks.

A cell cannot function properly without the nucleus, endoplasmic reticulum, Golgi apparatus, lysosomes, and mitochondria, among other organelles. As they make up the structural elements of the cell, aid in the movement of molecules inside the cell, and serve as enzymes to carry out metabolic events, proteins are also required for a cell to operate. Eukaryotic cells can perform a number of biological operations, including DNA replication, protein synthesis, and energy production, thanks to the help of these organelles and proteins.

Eukaryotic cells are those that have a membrane-enclosed nucleus and other organelles. The DNA of these cells is arranged into chromosomes within the nucleus, and they are typically much larger than prokaryotic cells. Eukaryotes are organisms like fungus, mammals, and plants.

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assuming no crossing over between the gene in question and the centromere, when do alleles segregate during meiosis?

Answers

Each daughter cell now has one allele for the gene in question, and it is these alleles that will segregate during meiosis I.

Meiosis I, the first meiotic division, is when alleles separate. Homologous chromosomes, a pair of chromosomes carrying the same genes, detach from one another and travel to the opposing sides of the cell during meiosis I. The alleles for that gene will segregate throughout this process of homologous chromosomal segregation if we assume that there is no crossing over between the gene in question and the centromere (the location where the chromosomes are joined). The homologous chromosomes align along the cell's equator during meiosis I and are forced apart by spindle fibres connected to the centromeres. Two daughter cells are created as a result, each of which has one homologous chromosome. Now that each daughter cell carries one copy of the relevant gene, meiosis I will see the segregation of these alleles.

It's vital to note that this scenario presupposes that the involved gene and centromere won't cross paths. Homologous chromosomes exchange genetic material during the process of crossing over, which leads to the recombination of alleles. The segregation of alleles may not be as simple as previously described if crossing over takes place.

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Do we all have the same DNA? Explain.

Answers

Answer

Yes, we share 99.6 percent of DNA

Explanation

The human genome is mostly the same in all people. But there are variation accounts for about 0.001 percent for each person's DNA and contributes to differences in appearance and health. Closely related people have more similar DNA. (Researched)

On the basis of the ingredients, which media types could support the growth of a heterotroph?

Answers

Heterotrophs are most commonly studied in bacteria that grow readily in media containing carbohydrates, proteins, or other complex nutrients such as blood.

What is Heterotroph?

A heterotroph is defined as an organism that cannot produce its own food, instead taking nourishment from other sources of organic carbon, mainly plant or animal matter whereas, in the food chain, heterotroph are primary, secondary and tertiary consumers, but not producers.

On the basis of the ingredients, bacteria containing media having carbohydrates, proteins, or other complex nutrients such as blood could support the growth of a heterotroph.

Thus, Heterotrophs are most commonly studied in bacteria that grow readily in media containing carbohydrates, proteins, or other complex nutrients such as blood.

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A client had an open reduction internal fixation of a distal radius fracture 3 months ago. The client developed complex regional pain syndrome 2 months after the surgery. Currently, the client has decreased functional use of the dominant upper extremity and is scheduled to begin an 8-week work hardening program. What should be the initial goal of this intervention?

Answers

The primary objective of this intervention is to investigate whether Complex Regional Pain Syndrome (CRPS), which is particularly common in the population of distal radius fractures (DRF).

It causes persistent pain and disability in many of its victims two to six years after it first manifests. Three months ago, an open reduction internal fixation of a distal radius fracture was performed on a patient. Two months following the procedure, the patient experienced complex regional pain syndrome.

The client will soon start an 8-week work hardening program and has currently reduced functional use of the dominant upper extremity. Chronic pain known as complex regional pain syndrome (CRPS) typically affects the arm or leg. CRPS frequently occurs following an injury, surgery, or stroke.

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1. during the evolutionary synthesis, biologists conclusively identified natural selection, gene flow, genetic drift, and mutation as the major causes of evolution within species. using the biological definition of evolution, explain how one of these forces causes populations, species, and higher taxa to evolve

Answers

One of the major forces of evolution identified during the evolutionary synthesis is natural selection. In its simplest definition, natural selection is the process by which certain traits become more or less common in a population over time due to their effect on an organism's ability to survive and reproduce.

For example, consider a population of birds in which some individuals have longer beaks and others have shorter beaks. If a new food source becomes available that can only be reached by birds with longer beaks, then the birds with longer beaks will have a greater chance of surviving and reproducing, passing on their trait to their offspring.

Over time, the proportion of birds with long beaks in the population will increase, while the proportion of birds with short beaks will decrease. This process of natural selection will cause the population to evolve, with longer beaks becoming more common.

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according to the diathesis-stress model, which person is most likely to experience a depressed state?that carry messages from one neuron to the next are called

Answers

According to the diathesis-stress model, an individual who is predisposed to mental illness, or has a "diathesis," is more likely to experience a depressed state when they are exposed to significant stress.

The diathesis-stress model proposes that both genetic and environmental factors contribute to the development of mental illnesses, including depression. A person with a genetic predisposition, or diathesis, to mental illness is more vulnerable to the effects of stress and may be more likely to experience a depressive episode when exposed to stressful life events. However, it's important to note that not everyone with a diathesis will experience a mental illness, and not everyone who experiences stress will develop a mental illness. It's the interaction between the diathesis and stress that increases the risk for mental illness.

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Does glycolysis produce more ATP than aerobic respiration?

Answers

Organisms can only split a 6-carbon glucose molecule into two 3-carbon molecules of pyruvate in the absence of oxygen. As we previously observed, glycolysis only produces two

What exactly does glycolysis entail?

A six-carbon sugar called glucose is broken into two atoms of a three-carbon saccharide pyruvate during the process of glycolysis. This multi-step process produces two molecules of free-energy-containing ATP, two molecules of pyruvate, two molecules of high-energy, electron-carrying Nadph, and two water molecules. The process of metabolising glucose is known as glycolysis.

Is oxygen required for glycolysis?

Yet, many anaerobic microorganisms that do not rely on air contain this route since glycolysis does not require oxygen. There are ten steps in glycolysis, and you might be interested in learning about each one individually depending on your hobbies and the classes you're taking.

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the ribs that are attached posteriorly to the thoracic vertebrae but are not attached anteriorly are known as

Answers

False ribs are defined as those that articulate directly with the sternum, whereas false ribs are defined as those that do not.

Which ribs are not anteriorly attached?

Each rib has a costal cartilage at the anterior end. True ribs (1–7) join the sternum directly at the costal cartilage. The false ribs (8–12) either indirectly or completely lack an attachment to the sternum.

What is the name for the ribs that are joined posteriorly but not anteriorly to the sternum?

The 11th and 12th ribs are known as floating ribs because they are not physically connected to the sternum. Because they don't directly connect to the sternum, ribs eight through ten are referred to as false ribs. The ribs and costal facet articulate at the level of the spine.

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