Which of the following ligation products will NOT yield a colony if transformed into E.coli ?
Plasmid with an insert
Plasmid with multiple inserts
Re-ligated Plasmids
Multiple inserts ligated together
None of the above

Answers

Answer 1

The correct answer is option C.

The ligation product that will NOT yield a colony when transformed into E. coli is the "Re-ligated Plasmids."

Re-ligated plasmids refer to plasmids that have been circularized without the insertion of any foreign DNA. In this case, there is no additional DNA sequence present to provide a selectable marker or any other functional element required for bacterial growth and survival.

Consequently, E. coli cells transformed with re-ligated plasmids will not be able to replicate and form colonies under selective conditions. In contrast, plasmids with an insert, plasmids with multiple inserts, or multiple inserts ligated together will contain the necessary DNA sequences for colony formation and can be successfully transformed into E. coli.


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Related Questions

In guinea pigs, white coat color is determined by the allele W. Black coat color is controlled by the allele w. Cross a white & black spotted pig with a black pig.

W = white

w = black

What percentage of the offspring will be black?

Answers

Answer:

About 75%

Explanation:

About 75% will have two ww alleles, while the rest will have Ww alleles.

75 percent I think that’s right

What does a mass extinction look like in the fossil record?​

Answers

There is less diversity hope this helps

Answer:

There is less diversity in the fossil record

Explanation: when compared to older rock layers the fossil record will be less diverse

the greatest genetic variation in a species is found in a offspring that forms by...
A:Asexual Reproduction
B:Sexual Reproduction
C:Budding
D:Cell Division

Answers

B: Sexual Reproduction

Which best describes the flow of genetic information?

A) Genetic information flows from gene to allele to protein
B) Genetic information is carried only by DNA
C) Genetic information flows from DNA to RNA to protein
D) Genetic information cannot be transmitted outside the nucleus

Answers

Answer:

It’s c I got it right

Explanation:

Answer:

C) Genetic information flows from DNA to RNA to protein

Explanation:

Astrology work help needed

Answers

Answer:

june 18 tell me if this is wrong i got it from goo gle

Explanation:

June 20 to 21 is the start of summer and longest day of sunlight in the Northern Hemisphere and the start of winter and shortest day of sunlight in the Southern Hemisphere. Though it might seem like the summer solstice would also be when the sun rises earliest and sets latest, it is not.

June 18 I’m guessing

What do you consider to be your culture? How do you feel about it?

Answers

Answer:

My culture is my Jewish culture.

Explanation:

I feel great about it.

What are the inputs and outputs of cellular respiration and photosynthesis?
carbon
dioxide
oxygen
energy
Mater
sugar
Photosynthesis
Cellular Respiration
Inputs
Outputs

Answers

Answer: inputs of cellular respiration? Glucose, oxygen

outputs of cellular respiration? Carbon dioxide, water, energy (ATP)

In photosynthesis, water, carbon dioxide, and energy in the form of sunlight are inputs, and the outputs are glucose and oxygen.

Explanation:

Soil forms as ______ is broken down by weathering.

Group of answer choices

water

rock

humus

magma

Answers

Answer:

Humus

Explanation:

Humus is dark, organic material that forms in soil when plant and animal matter decays. When plants drop leaves, twigs, and other material to the ground, it piles up. This material is called leaf litter. When animals die, their remains add to the litter. Over time, all this litter decomposes

how many checkpoints are there in mitosis

Answers

Answer:

3

Explanation:

hope that helps!!!!!!!

Answer:

three

Explanation:

There are three major checkpoints in the cell cycle: one near the end of G1, a second at the G2/M transition, and the third during metaphase.

Firebreathing (F) is dominant in dragons. NON-firebreathing (f) is recessive.a)You have a firebreathing dragon. What are its possible genotypes

Answers

FF or Ff, since these traits that are displayed are the dominant ones these are the only possible genotypes for this dragon

1. DNA 2. Helicase 3. DNA Polymerase 4. DNA Replication 5. Pyrimidine a. Double stranded molecule that has a deoxyribose sugar. b. The end-product of this process are two identical copies of DNA. c. Enzyme that breaks hydrogen bonds to unwind and unzip DNA. d. Nitrogen containing base that has a single ring as its molecular structure. Thymine and Cytosine are an example of this type of molecule. e. Enzyme that covalently bonds sugars and phosphates during DNA replication.

Answers

Answer:

1. DNA-A.Double stranded molecule that has a deoxyribose sugar.

2. Helicase- C. Enzyme that breaks hydrogen bonds to unwind and unzip DNA

3.DNA Polymerase- E. Enzyme that covalently bonds sugars and phosphates during DNA replication

4.DNA Replication-B. The end-product of this process are two identical copies of DNA.

5.Pyrimidine-D. Nitrogen containing base that has a single ring as its molecular structure. Thymine and Cytosine are an example of this type of molecule.

Explanation:

Mendel's law of segregation is describing or explaining which process?

a mitosis
b. binary fission
c. meiosis
d. asexual reproduction

Answers

Answer:

meiosis

Explanation:

because Mendel's law of segregation states that: “During the formation of gamete, each gene separates from each other so that each gamete carries only one allele for each gene.” Same applies to meiosis the chromosomes separate.

which of the following is a stimulus, which response?
A. The recess bell ringing in a elementary school
B. Your mouth watering at sight of food on a plate
C. A sudden drop in air temperature
D. A flu virus entering your body
E. Getting "butterflies" in your stomach before giving a speech

Answers

Answer:

A. Stimulus

B. Response

C. Stimulus

D. Stimulus

E. Response

Explanation:

a description of the forest ecosystem

Answers

A forest ecosystem is a natural woodland unit consisting of all plants, animals in micro organisms in that area functioning together with all nonliving physical factors of the environment. The forest ecosystem is very important

It's forensic science

Answers

Answer: Loops: 65%, whorls: 30%, and arches: 5%

Explanation:

The loops are 65% in the population, 30% are whorls and arches are 5%. The fingerprints are the patterns which include the friction ridge characteristics also called minutiae characteristics. These can be found in the soles of feet and palms of hand. At the distal end of finger the typical friction ridge pattern forms patterns like loops, whorls, arches, and composites which are patterns including two or more patterns. The loops are most common in the population the ridges emerge and end on the same side in this pattern. The loops are radial or ulnar loops depending upon the side in which pattern ends of the side of the bone thumb in case of radial and little finger in case of ulnar loop.

DNA is an example of a nuclei case acid true or false

Answers

Answer:

The term nucleic acid is the overall name for DNA and RNA.

Explanation:

They are composed of nucleotides, which are the monomers made of three components: a 5-carbon sugar, a phosphate group and a nitrogenous base. ... The two main classes of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).

Offline, create a complete pictogram that represents (1) the various sources of energy; (2) the percentage of global consumption for each as shown in the video; and (3) what each source is used for (electricity, transportation, heat). Use the illustration from the video as a guide.

Answers

Answer:

Solar energy, geothermal energy, wind energy, hydropower, nuclear and fossil fuels

Explanation:

Various sources of energy are solar energy, geothermal energy, wind energy, hydropower, nuclear and fossil fuels. The various sources of energy consumption for the generation of electricity was 41% produce from coal, 22% natural gas, 11% nuclear, 16% hydro, 6% from other sources such as solar, wind, geothermal, biomass, etc and 4% from oil.

A 154-lb adult man performs a moderate level of physical activity and regularly consumes 2700 Calories a day. State whether the weight of the man will most likely decrease, increase, or remain the same. Use information from the data table to explain your answer.

The weight of the man will...
Explanation...

Answers

Answer:

Increases.

Explanation:

A 154-lb adult man regularly consumes 2700 Calories a day and performs a moderate level of physical activity, the weight of that individual increases because a 154-lb adult man needs only 2450 Calories a day and that person consumes 2700 Calories a day which is higher than their needs so these extra calories stored in their body and as a result the weight of that person increases.

Four differences between viruses and bacteria​

Answers

Answer:

bacteria :

1. need microscope to see them.

2. need warmth moisture nutrients.

3. saprophyte or parasite.

4. can be harmful or useful.

viruses:

1. smaller than bacteria.

2. spend on living host.

3. always parasite.

4. where is harmful.

I need help so I'm giving 100 points away if you help with this question
The use of grass filter _______ can help block nitrates and pesticides from washing into the water system in runoff from cultivated fields

there are no answer choices so please answer slowly and think about it

Answers

Answer:

buffer zones

Explanation: rather than planting the crop right up to the water you leave some as grass or actually plant grass in that buffer zone to filter out and prevent chemicals from reaching the water system

Question 1/7 The Nile River carries sediments to the ocean. Over time, the sediments are compressed as more sediments are deposited on top of them. Which type of rock will be formed?​

Answers

Answer:

Sedimentary Rock

Explanation:

Sedimentary rocks are formed from pieces of sediments or other existing organic matter or rock.

The sedimentary rock formation process begins with weathering which involves the breakdown of the sediments into small fragments. The next process is erosion, where water like the Nile River carries them to other places -  in this case, the Ocean.

Over time, the sediments settle and become compressed as more sediments are deposited on top of them.

This leads to the formation of Sedimentary Rocks.

PLEASE CHOOSE ALL 3 CORRECT ANSWERS! WILL MARK BRAINLIEST IF CORRECT

Which of the following are true of the transfer of thermal energy in the oceans? Select the three correct answers. (2 points)

Energy is absorbed more than radiated near the equator.

Energy is carried from the equator to poles in deep ocean currents.

Energy is carried from the equator to poles in surface currents.

Energy is radiated more than absorbed near the equator.

Energy is absorbed more than radiated near the poles.

Energy is radiated more than absorbed near the poles.

Answers

Answer:

Energy is absorbed more than radiated near the equator.

Energy is radiated more than absorbed near the poles.

Energy is absorbed more than radiated near the poles.

Question 2 (1 point)
What type of Chemical bond holds the bases together?

a) Hydrogen bonds
b) Structural bonds
c) Nucleic bonds
d) Polar bonds

Answers

the answer is A, hope this helps !

In asexual reproduction, 2 parents each pass copies of all of their chromosomes to the offspring. true or false

Answers

Answer:

False.

Explanation:

How has technology such as telescopes, satellites, computers, and GPS units affect the scope of scientific discovery?

Answers

Answer:ghhjhjhkjhkjkjhkhExplanatio

How was osmosis involved in the cause of Clark's seizures?

Answers

Answer:

after Clark was treated with a hypertonic solution? increased Na concentration in matrix of brain caused water from blood to move in to the brain which increased the matrix pressure. Pressure caused the neuron firing rate to increase which increased the likelihood of seizures.

Describe at least two ways in which nitrogen is important to biological processes. (Hint: Find the biological chemicals that contain nitrogen.)

Answers

Answer:

Nitrogen is a crucially important component for all life. It is an important part of many cells and processes such as amino acids, proteins and even our DNA. It is also needed to make chlorophyll in plants, which is used in photosynthesis to make their food. Nitrogen is a key element in the nucleic acids DNA and RNA, which are the most important of all biological molecules and crucial for all living things.  Without amino acids, plants cannot make the special proteins that the plant cells need to grow. Without enough nitrogen, plant growth is affected negatively.

I hope this helped out some~ <3

-Dream

in which Domain & Kingdom would scientists classify an organism, whose cells contain a nucleus, has cell walls made of chitin, and does not have chloroplasts?

Answers

Answer:

The correct answer is -

Domain: eukarya

kingdom: fungi

Explanation:

A cell with a nucleus and cell wall made up of the chitin and does not have chloroplast, are all the characteristic features of an organism of kingdom fungi which is a group of eukaryotic organisms.

The cells with the nucleus are grouped in the domain eukarya while the prokaryotic organism does not carry a nucleus in their cell and divided into Archaea and bacteria. The chitinous cell wall without any chloroplast in the cell is one of determining features of the fungi kingdom.

PLEASE HELP THE ASSIGNMENTS DUE TODAY
Which of the following statements about human population growth is TRUE?

(2 Points)

a. The population size is growing, and growth rate is increasing.

b. The population size is growing, and growth rate is decreasing.

c. The population size is decreasing, and growth rate is increasing.

d. None of these is true.

Answers

Answer:

The population size is growing, and growth rate is increasing

Answer: a

Explanation:

Why do you think eating beef and dairy from cows that are fed corn and soy has such a high environmental impact compared to eating vegetables?

Answers

Answer:

because it has more nutriants and helps them get fat more.

Explanation:

read it

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Suppose that f and g are functions that are differentiable at x = 1 and that f(1) = 2, f '(1) = 1, g(1) = 2, and g'(1) = 3. Find h'(1). h(x) = (x2 + 5)g(x) a repeating group is a set of one or more fields that can occur only once in a single record, with each occurrence having the same value. true or false? The ligands of endosomal Toll-like receptors are _____.a. lipids of Gram-negative bacteriab. flagellin proteins of bacteriac. lipids of Gram-positive bacteriad. zymosan of fungie. nucleic acids of viruses and bacteria If the process capability ratio of a centered process is 0.7, and the process mean is 76, what is the average amount of defects per 1 million outputs?a.16395b.35729c.71861d.51176 Your math teacher owns 3 pairs of pants and 3 pairs of shoes. He owns a pair of gray pants, green pants, and blue pants. He owns black shoes, brown shoes, and tan shoes. If he randomly chooses one pair of pants and one pair of shoes, what is the sample space of possible combinations of pants and shoes that he could wear on a typical school day? Discuss, in detail, at least three ways Social Media has changed the way we learn about and select products. Find the lateral surface area What are genes (DNA) and why are they important in cell specialization? Which method proved to be fatal at the galeras volcano? a 42-year-old woman has a developed a solid and quite firm thyroid mass that is approximately 1 cm diameter by palpation. she does not have any hoarseness, difficulty breathing or swallowing, or symptoms of thyroid disease. she has never been exposed to radiation and has no history of cancer, nor does she have a family history of thyroid or other cancers. a thyroid panel is within normal limits. what is the next step in the care of this patient? marjalayne is 10. she is beginning to show some unusual, even disturbed feelings and actions. based on this information alone, marjalayne may be starting to show signs of: a leader who gives an individual employee or groups for making the decision within some sets of specified boundary conditions is using which decision-making style .Draw the product formed whenC6H5N2+Clreacts with each compound The number of moose in a national park is modeled by the function Mthat satisfies the logistic differential equation M = 0.6M (1 M), where tis the time in years and M (0) = 50. What is lim M (t)? 4000 A 50 B 200 C 500 D 1000 E 2000 let an = 4n 5n 1 . (a) determine whether {an} is convergent or divergent. if it is convergent, find its sum. (if the quantity diverges, enter diverges.) What is the theme of the poem A Hero by Katherine Tynan create 2 random 4x3 arrays and calculate the sum of their crrosponding elements. use vectorization for sum calculation. There are eleven court districts in Nevada. In which district is Reno/Sparks? the standardized process described in the scor model that includes the selection of raw material suppliers is If 10.0 liters of oxygen at STP are heated to 512 C, what will be the new volume of gas if the pressure is also increased to 1520. 0 mmHg?