What is the significance of Non-Homologous end joining and homology directed
repair with respect to the use of CRISPR-Cas9 technology:

Answers

Answer 1

There are fewer mistakes or mutation hazards if the DNA template used for repair is the same as the original, undamaged DNA sequence.

Compared to non-homologous end joining, homology guided repair has some advantages.

When chromosomal DSBs arise, the cell uses two sorts of repair pathways:

non-homologous end joining (NHEJ) and homology-directed repair (HDR). Fundamentally,

NHEJ-break ends can be ligated without a template but HDR-breaks require one to direct healing.

Which is more useful: NHEJ or HDR?

Both of these repair processes are frequently active in cells, albeit the HDR pathway often works poorer than the NHEJ pathway in the absence of a homologous template.

Learning about DNA Templates

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Related Questions

Explain how a male / female contraceptive pill works

Answers

When the sperm enters the female, the chemicals from the pill kill the sperm. Therefore the female won’t get pregnant.

I hope this helps.

Answer:

When the sperm enters the female, the chemicals from the pill kill the sperm. Therefore the female won’t get pregnant.

Explanation:

right above me.

________ cells lack a membrane-enclosed nucleus.

Answers

Answer:

Prykaryotic

Explanation:

Prykaryotic cells lack a membrane-enclosed nucleus.

What hypothesis explains why all birds share similar characteristics?

Answers

Answer:

this is a common ... saw incredible similarities between birds and the theropod dinosaurs (especially ... Like all other reptiles, birds have scales (feathers are produced by tissues similar to ... for theropods are often more serious for the "thecodont" pseudo-hypothesis.

Explanation:

Answer:

The modern version of the "from the ground up" hypothesis argues that birds' ancestors were small, feathered, ground-running predatory dinosaurs that used their forelimbs for balance while pursuing prey

Explanation:


What structure has become useless through evolution but still exists,
typically in a much reduced form?

Answers

Answer:

A "vestigial structure" or "vestigial organ" is an anatomical feature or behavior that no longer seems to have a purpose in the current form of an organism of the given species. Often, these vestigial structures were organs that performed some important functions in the organism at one point in the past.

Explanation:

which of the following is the most complex
according to their cellular level of organization? A.
Hair B. Euglena C. Hydra D.Heart. bplease give explanations.​

Answers

d.corazon.ya que sin el no habria vida

In the first step of pollination, _____ from the male anther is transferred to the female _____. The pollen grain is then carried down the _____ to the ovary. Inside it meets with an ______. This is the process of fertilization.

WILL GIVE BRAINLIEST IF YOU HELP, IT'S DUE TMRWWW

Answers

Answer:

1.  pollen grains

2. stigma

3. style

4. egg cell of an ovule

Through what process can scientists artificially change the DNA code of organisms?
A)DNA fingerprinting
B)Polymerase Chain Reaction
C)Cloning
D)Recombinant DNA

Answers

Answer:

cloning is the right answer

Through Recombinant DNA scientists can artificially change the DNA code of organisms.

What is recombinant DNA?

Recombinant DNA is a term used to describe the process of creating new combinations of DNA from different sources. This is done by cutting DNA molecules from different sources and joining them together, creating a recombinant molecule.

This process is often used in biotechnology for applications such as creating new vaccines, improving crop yields, and producing therapeutic proteins. Recombinant DNA technology plays a crucial role in many areas of modern biology and medicine, including genetic engineering, gene therapy, and drug discovery.

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What is defined as the variable in a controlled experiment that is purposely changed or manipulated? PLEASE HELP!!

constant
independent variable
controlled variable
dependent variable

Answers

Answer:

Independent Variable

Explanation:

Cape Town, South Africa has a significant water shortage. The amount of water available is not sufficient to support the current human population of the city.
Which three factors most likely contributed to the growing water shortage?
- Strict water management policies.
- Naturally-occurring drought conditions.
- Underdeveloped alternative and supplemental water sources.
- Migrations of residents out of the city and into surrounding areas.
- Higher demand for water than natural reservoirs accumulate each year.

Answers

Explanation:

The city's water supply shortage seems to be the result of a combination of physical, social, and political factors. Well below-average rainfall patterns, the increasing unpredictability of weather conditions due to anthropogenic climate change impacts, population growth, and government mismanagement have produced one of the worst urban water crises in recent memory.

here is the reason your explanation from the water shortage now just figure out what the factors are in your answers I would say it's naturally occurring drought conditions because it says on here well below average rainfall patterns and climate change

Water shortage can occur as a result of mismanagement practices by humans or as a result of natural occurrences such as droughts.

What is water shortage?

Water shortage refers to a situation in which there is not enough water to meet the daily water needs of the human, animal and plant population in a particular area.

The water shortage issue in Cape Town, South Africa could have been as a result of:

Naturally-occurring drought conditionsUnderdeveloped alternative and supplemental water sourcesHigher demand for water than natural reservoirs accumulate each year

Therefore, water shortage can occur as a result of mismanagement practices by humans or as a result of natural occurrences such as droughts.

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A decomposer is an organism that gets its energy from ____________.

Group of answer choices

the food it produces during photosynthesis

the prey it hunts and kills

from other living organisms

dead plant and animals

Answers

A decomposer is dead plant and animals I’m sorry if I’m wrong have a good day/night!

Some organisms reproduce sexually. Other organisms reproduce asexually. What are some benefits of asexual reproduction?

1.It It benefits organisms that are immobile

2.immobile.It gives offspring the genetic traits to survive in a particular environment

3. It uses less energy.

4. energy.All of of the above above

Answers

Answer:

3

Explanation:

because you don't have to ...you know

Answer:

It is 4 all of the above.

Explanation:

Yes because I got it right on test.

Which action is necessary for a solar eclipse to occur?
The Moon needs to be full.
Earth needs to pass in front of the Sun.
The Sun needs to pass in front of the Moon.
The Moon needs to pass between Earth and the Sun.

Answers

The Moon need to pass between Earth and the Sun is the answer

In 4-5 sentences, explain why it is important for our cells to undergo the cell cycle.

Answers

Answer:

If our cells don't undergo the cell cycle, our body will run out of cells and we'll maybe suffer from diseases and the cells in our body isn't able to fight the disease and we might die. Our body is made up of good cells which protects our health.

Need Help ASAP plz will give Brianliest. Digestive juices contain enzymes, water and some
other substances. Name two of these other substances.
State which digestive juice contains them, and state
what function they perform.

Answers

Answer:

Hydrochloric acid  Prepares protein for  digestion  (denaturation)  Activates enzymes  and  “Disinfection”

Pepsin (an enzyme)  Begins protein  digestion

Gastric lipase  Some lipid digestion

Gastrin (hormone)  Stimulates gastric  secretion and  movement

Intrinsic factor (a  glycoprotein)  Needed for  absorption of vitamin  B12

Explanation:

Which of the following increases the population size?

a. favorable light and temperatures

b. inadequate food supply

c. inability to complete

d. inability to migrate, hide or defend

e. inability to resist disease

Answers

Favorable light and temperatures can increase population size

Inadequate food supplies can lead to starvation and/or death

If a population can’t migrate, hide or defend, they will likely die

Populations need to be able to resist disease or they will be wiped out

Given the sequence of DNA nucleotide bases TTAGCCTTG, give the complementary strand of RNA.

Answers

Answer:

AAUCGGAAC

Explanation:

Notice how it says RNA, which means T is replaced with U

Complementary sequences are A and U, and G and C

How do mosses and ferns reproduce?


with spores


with seeds


with eggs


with sperm
I think the answer is -> SPORES <-

Answers

It is with sperm and eggs, which are housed in spores in both species, so I’d say spores is the safest answer.

A population of rabbits lives in a grassy field that is lush and green in summer and covered in snow in winter. At the start of the summer, 75% of the population are white rabbits, and the remaining 25% of the population are brown rabbits. In rabbits, white (W) is dominant over brown (w). During the summer, the field where they live experiences a drought. The summer drought lasts for two years, through many generations of rabbits.
Which prediction BEST estimates the expected percent of white and brown rabbits in the population at the end of the drought?

Answers

Answer:

The rabbit population will consist of 35% white rabbits and 65% brown rabbits.

Explanation:

hope this helps!

The rabbit population will consist of 35% white rabbits and 65% brown rabbits.

What are the limiting factors of the rabbit population?

Until a limiting factor, such as low reproduction, food scarcity, predation, disease, and/or weather conditions restrict populations, the population tends to grow.

Some density-dependent limiting factors are the spread of pathogens, competition for nesting locations, the destruction of habitat, increase in the population of predators. Several predators, including raptors and coyotes, use rabbits as food, but in urban and suburban areas, cats and dogs pose the biggest threat. Some abiotic limiting factors are drought, flood, earthquake, and all types of natural disasters.

Thus, the rabbit population will consist of 35% white rabbits and 65% brown rabbits.

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A helium atom that has only two electrons will have its electrons in which orbital(s)?

Answers

1s orbital

Explanation:

The electron has to go into the first energy level, in the 1s orbital. Helium, with two protons, will have two electrons. These are placed one at a time into the 1s orbital. This makes helium a very contented atom.

List and explain the characteristics of living things

Answers

Answer:

Life process

Movement

Respiration

Sensitivity

Growth

Reproduction

reason:

Life process

Explanation

Earthworms

Movement

All living things move in some way. This may be obvious, such as animals that are able to walk, or less obvious, such as plants that have parts that move to track the movement of the sun.

Earthworms use circular and longitudinal muscles to move through soil or along surfaces.

Respiration

Respiration is a chemical reaction that happens within cells to release energy from food.

The food that earthworms eat supplies their body with energy-rich molecules such as glucose. On entering the cells of their body, these molecules are broken down in a series of steps to release energy to be used by the body, producing carbon dioxide and water as waste products.

Sensitivity

The ability to detect changes in the surrounding environment.

Earthworms have light-sensitive cells scattered in their outer skin. Their skin cells are also sensitive to touch and chemicals.

Growth

All living things grow.

Earthworms hatch from eggs and can grow up to a metre or more in length! Some earthworms are also able to regrow small parts of their body that have been lost or injured.

Reproduction

The ability to reproduce and pass genetic information onto their offspring.

Earthworms have both sperm and eggs within their bodies (they are hermaphrodites) but they cannot self-fertilise and need to mate with another individual. After mating, a cocoon containing the fertilised eggs is deposited in the soil.

Excretion

Getting rid of waste.

Earthworms excrete waste from their anus – the last segment of their body.

Nutrition

The intake and use of nutrients. This occurs in very different ways in different kinds of living things.

Earthworm nutrition comes from a variety of sources, depending on their species. Food types include manure, compost, plant material, fungi, microorganisms and decaying animals. They take in food through their mouths.

Despite the rampant depletion of trees in the natural forest in Ghana,it is still worth using wood as the main construction.Disscuss

Answers

Answer:

Hope this helps!

Explanation:

Forest resources support livelihood of people in jurisdictions in which forest is located. However, the forest size is gradually depleting continuously. The lack of information about the specific agents and forces of forest depletion is worrying. This is due to the inter-generational and intra-generational equity notions that affect mankind. As such, there is the need to maintain such resources on a non-declining value basis so as to maintain human welfare over time. Thus, the objective of this study is to empirically examine the determinants of forest depletion in Ghana over the 1970-2012 period in Ghana. This study employs multiple regression concepts to examine the effects of the determinants on forest depletion. The findings of the study indicate that gold extraction for export, population growth and logging increase the rate of forest depletion for the study period. More significantly, population growth reflected in settlement expansion, agriculture purposes, increased use of fuel wood, etc have greater effect on forest loss in Ghana. This suggests that, protection of the forest is necessary and sufficient condition for economic growth. As a result of these, both government and policy-makers need to safeguard the forest estate, by increasing afforestation schemes and also using part of the returns from gold exports to regenerate the forest.

Answer:

The main cause of deforestation is agriculture (poorly planned infrastructure is ... In 2019, the tropics lost close to 30 soccer fields' worth of trees every single minute. ... of Texas could be lost to rampant deforestation, according to a WWF report. ... These fires not only alter the structure and composition of forests,

Explanation:

What’s one indirect consequences of building Seawalls

Answers

The shoreline habitat is destroyed through loss of sand. -is one indirect consequence of building seawalls. The shoreline habitat is destroyed through loss of sand.

Answer:

The shoreline Habitat is destroyed through loss of sand.

Explanation:

I have no idea how to do this please help!

Answers

Answer:

What are you supposed to do?

Explanation:

Please answer!!

Read the paragraph from the section "Types Of
Isolation."There are biological properties of organisms
that prevent breeding across different species. They are
known as reproductive isolating mechanisms (RIMs). A
mechanism is a process or method. Which option is the
BEST definition of "properties" as it is used in the
paragraph above?

Answers

The correct answer is B. Traits or features that take effect.

Explanation

Property is a word to designate any quality that identifies identifying something or someone, distinguishing it from its peers. It can refer to different issues such as its characteristics, capabilities, possibilities, physical appearance, among others. According to the text, "There are biological properties of organisms that prevent breeding across different species". So the answer is B. Traits or features that take effect.

Genes for body color (B black dominant to b yellow) and wing shape (C straight dominant to c curved) are located on the same chromosome in flies. If single mutants for each of these traits are crossed (i.e. a yellow fly crossed to a curved-wing fly), and their progeny is testcrossed, the following phenotypic ratios are observed among their progeny.

Black, straight 17
yellow, curved 12
black, curved 337
yellow, straight 364

Required:
Calculate the map distance between B and C.

Answers

Answer:

Map distance = Genetic distance, GD = 3.9 MU ≅ 4 MU

Explanation:

The more separated two genes are, the more chances of recombination there will be.  The closer they are, the fewer chances of recombination there will be. Two genes that are very close will have a few recombination events and are strongly bounded.    

To analyze the recombination frequency, we have to know that:

1% of recombination = 1 map unit = 1centi Morgan = 1,000,000 base pairs. And that the maximum recombination frequency is always 50%.  

The map unit is the distance between the pair of genes for which every 100 meiotic products one of them results in a recombinant one.  

Knowing that the genes are linked, we can calculate genetic distances between them. First, we need to identify the genotypes of the parental gametes with the ones of the recombinants. We can recognize the parental gametes in the descendants because their phenotypes are the most frequent, while the recombinants are the less frequent. So:

Parentals:

black, curved 337yellow, straight 364

Recombinants:

Black, straight 17yellow, curved 12

To calculate the recombination frequency we will make use of the next formula: P = Recombinant number / Total of individuals.

P = Recombinant number / Total of individuals.  

P = 17 + 12 / 337 + 364 + 17 + 12

P = 29 / 730

P = 0.039

The genetic distance (GD) will result from multiplying that recombination frequency (P) by 100 and expressing it in map units (MU).

GD = P x 100

GD = 0.039 x 100

GD = 3.9 MU ≅ 4 MU

In peas, round shape is dominant (R) over wrinkled (r) and yellow color is dominant (Y) over green (y). If both parent plants are heterozygous for both traits,
what is the probability of an offspring's phenotype being round and yellow?

HELP I NEED A NUMBER OUT OF 16

Answers

Answer:

9/16

Explanation:

RrYy x 2

Answer:

9 out of 16 (also 9/16)

Explanation:

Count the likeliness of the offspring to be round:

RR, Rr, rR, rr

round shape is 3 out of 4 times

Count the likeliness of the offspring to be yellow:

YY, Yy, yY, yy

yellow color is 3 out of 4 times

Multiply the likeliness of being round and being yellow (since they are two independent probabilities, we are using multiplication rule):

3/4 * 3/4 = 9/16

Make me the brainliest ;)

What is true of adaptations?
the answer is
They are passed along to offspring

Answers

Answer:

They are passed along to offspring

Explanation:

HOPE IT HELPS YOU

water is a renewable resource that is constantly generated through the water cycle . in what areas might the water cycle be most limited ?
A) Canada .
B) south Africa .
C) south America .
D) Northern Africa .

Answers

I think c I’m not sure

Explain how to revise the following DNA model to form mRNA


AACCTGGGACTATGAT

Answers

Uuggacccugauacua is the answer

The top layer of the soil in the diagram is composed MAINLY of
A)
sand and clay.
B)
solid bedrock
C)
many types of minerals.
D)
decaying organic material.

Answers

Where’s the diagram?
It’s A or D

Answer:

?

(there is no diagram)

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