What is the principle of size exclusion chromatography?​

Answers

Answer 1

Answer:

The underlying principle of SEC is that particles of different sizes elute (filter) through a stationary phase at different rates. This results in the separation of a solution of particles based on size.

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What Is The Principle Of Size Exclusion Chromatography?
Answer 2

Size exclusion chromatography (SEC) separates molecules based on their size by filtration through a gel.

What is size exclusion chromatography?

Size exclusion chromatography (SEC) splits molecules based on their size by filtration via a gel. The gel consists of spherical beads including pores of specific size distribution. Separation happens when molecules of various sizes exist included or excluded from the pores within the matrix.

Size exclusion chromatography exists named gel filtration chromatography because the gel effectively allows for the filtering of molecules from a sample based on molecular size. However, unlike other methods, the larger molecules elute first.

Size-exclusion chromatography (SEC), also understood as molecular sieve chromatography, exists a chromatographic procedure in which molecules in solution exist separated by their size, and in some cases molecular weight. It exists usually applied to large molecules or macro molecular complexes such as proteins and industrial polymers.

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Related Questions

13: Mycobacteria are stained with
a. Gram's staining
b. Simple staining
c. Both a and b
6. Ziehl - Neelsen's staining​

Answers

Answer:

d. Ziehl - Neelsen's staining

Explanation:

The mycobacteria is stained with the Ziehl - Neelsen's staining. Hence, option (d) is the correct answer.

If 622 water molecules were removed during the synthesis of a specific polysaccharide, how many monomers were joined together to make this molecule? need help please and thank you ​

Answers

Answer:

623 monomers

Explanation:

In a dehydration synthesis reaction, one water molecule is removed for every bond that is created between 2 monomers.

Linking 3 monomers together would remove 2 water molecules.

Following this pattern, the number of water molecules removed will always be one less than the number of monomers that were joined together.

So, if 622 water molecules were removed, then 623 monomers were joined together.

The answer is 623 monomers.

Which of the following could be a characteristic of the fossil that is most closely related to humans? S-shaped back bo...

Answers

Answer: S-shaped back bo

Explanation: That was the only choice

Answer:

S-shaped back bone

Explanation:

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Explain the processes that occur in the nucleus.

Answers

Answer:

A nuclear reactor is driven by the splitting of atoms, a process called fission, where a particle (a 'neutron') is fired at an atom, which then fissions into two smaller atoms and some additional neutrons. ... The fissioning of atoms in the chain reaction also releases a large amount of energy as heat.

define cell and atom​

Answers

Answer:

hope it is helpful to you

Explain why it is not advisable for two sickle cell carriers to marry.​

Answers

Answer:

it results in pleiotropic effect and caused death of an offspring.

All of the following factors contribute to the differences in complex trait risk predictions between different laboratories, EXCEPT ____________. differing methods of combining risk from multiple genetic variants selection of different genetic variants for risk determination different assessments of environmental factors genotyping of different variants

Answers

Answer:

different assessments of environmental factors

Explanation:

In humans, for predicting the future phenotype of any individual, the PSR is used.

A trait may be defined as the state of a character that is a distinct variation of the phenotypic characteristic of any organism.

The following factors contributes to the differences in the complex trait risk predictions in the different laboratories :

-- differing methods of the combining risk from the multiple genetic variants.

-- genotyping of the different variants.

-- selection of the different genetic variants for such risk determination.

Thus the factor which does not contribute is :

"different assessments of the environmental factors."




2. Explain two different ways that you could classify the following
items: banana, lemon, sandwich, milk, orange, meatball, salad.

Answers

Answer:

Two different ways that you could classify the following  items: banana, lemon, sandwich, milk, orange, meatball, and salad, are the following:

Fruits and not fruits, where the fruits would be banana, lemon and orange; and the non-fruits would be sandwich, milk, meatball and salad.

Food of animal and vegan origin, where the food of animal origin is sandwich, milk and meatball, and the vegan food is banana, lemon, orange and salad.

The following food items can be classified based on either plant or animal origins.

Classification of food products

Food is a substance that contains nutrients that is required for the growth and maintenance of the living organisms.

Food can be gotten from various sources which includes plant and animal sources.

Examples of plant sources are banana, lemon, sandwich, orange and salad

Examples of animal sources are milk and meatball. This is because it can be processed from cow and ram.

Therefore, the following food items can be classified based on either plant or animal origins.

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2. What is the percentage likelihood that the couple will have a child that has the allele for cystic fibrosis

Answers

Answer:

the answer I got for the question you asked is 75%

define cell and atom​

Answers

Answer:

Cell: All living organisms are made up of cells, which are the basic building components. Cells also carry the body's genetic material and have the ability to replicate themselves. Cells are made up of several components, each of which serves a particular purpose. Organelles, for example, are specialized structures that perform specific activities within the cell.

----------------

Atom: Protons, neutrons, and electrons are the three subatomic particles that make up an atom. The nucleus, which is made up of protons and neutrons, is at the core of the atom, while electrons float around in a tiny cloud above it.

-------------------------

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have a great day!!

. Imagine the following scenario. A bacteriophage taken for the treatment of cholera infects a cholera-causing bacterium and temporarily enters the lysogenic stage. When the virus re-enters the lytic cycle, it includes some pieces of the bacterial DNA in its own genome. Specifcally, this DNA encodes information for making the cholera toxin. Imagine some of the possible consequences of this scenario. What will happen to the next bacteria that the virus infects

Answers

Answer:

The virus will transfer the DNA encoding cholera toxin to the next bacteria it infects, which will make that bacteria cholera-causing.

Explanation:

The process described in this question is a kind of gene transfer in bacteria called TRANSDUCTION. Transduction is the transfer of fragments of DNA from one bacterium to another via a virus called bacteriophage.

As stated in this question, the virus (bacteriophage) infects a cholera-causing bacterium. The bacteriophage moves from lysogenic cycle to lytic cycle and includes some pieces of the bacterial DNA, which encodes information for making the cholera toxin, in its own genome.

This means that the cholera-making toxin DNA is now a part of the virus's genome and hence, will transfer it to the next bacteria it infects in a process called TRANSDUCTION. This will make that bacteria a cholera causing bacteria.

Which product comes from protist

A. Yeast in sourdough bread

B. Mushroom on pizza

C. Cork in bulletin boards

D. Carrageenan in milk

Answers

Answer:

C. The cork in bulletin boards

Explanation:

Cork is made primarily from a plant called Quercus suber (the cork oak).

imagine that you are conducting tests shortly after a nuclear accident.Using potted moss plants as you experimental organisms,design a experiment to test the hypothesis that the frequency of mutations decreases with the organisms distance from the source of radiation

Answers

Answer:

Take two potted plants and provide equal conditions to both.

Explanation:

Two potted plants i.e. one is placed near the nuclear accident site while on the other hand, the other is placed far away from the nuclear accident. Provide equal conditions to both plants so that we can find out the hypothesis that  the frequency of mutations decreases with the organisms distance from the source of radiation. The result shows that the potted plant that is located near to the nuclear accident site having high frequency of mutation as compared to other plant due to the radiation present at that location.

Which of the following terms describes the distribution of a particular allele
within a population?
A. Allele dominance
B. Allele strength
C. Allele frequency
D. Allele density
Please help me

Answers

Answer:

Option : B Allele frequency...

Explanation:

The answer is allelic frequency. This is also the fraction of a particular allele of a gene in the population. please marks me as brainliests..please for my answer please...

In mice, apricot eyes is recessive to black eyes. Tail length is governed by another gene, linked to the eye color gene. Long tails is dominant to short tails. To determine the distance between the two genes, a double heterozygote is mated in a testcross and the classes of progeny produced were as follows:
Apricot eyes, Long tails 33
Apricot eyes, Short tails 20
Black eyes, Long tails 17
Black eyes, Short tails 30
Determine whether the heterozygous parent is in the cis or trans arrangement.
a. Cis
b. Trans

Answers

Answer:

trans

Explanation:

From the given information:

The study observes the genes present in mice for eye color and tail length. Since both genes are linked, it implies that they exist in the same chromosomes.

Black eyes is dominant over apricot eyes

Let Black eye be B and apricot eyes be b

Long tail is dominant over short tail

Let long tail be L and short tail be l

If double heterozygote(homoozygous-recessive) engage in the testcross

Then:

From the result given:

The parental combinations are:

Apricot eyes, Longtail  (bL / bl) =  33

Black eyes, Short tails (Bl / bl) = 30

The recombinant genes are:

Black eyes, Long tails (BL / bl) =  17

Apricot eyes, Short tails (bl / bl) = 20

The recombination frequency relates to the distance between the two genes which can be computed as:

= (20+17)/100

= 37%

Thus; the heterozygous parent is in trans arrangement.



What does a single colony of a pathogenic microbe represent?

Answers:
A clone
A species
A serotype
All answers are correct
No answers are correct
Question 9

Answers

Answer:

clone

Explanation:

Write an experiment to show that sunlight is necessary for photosynthesis.

Answers

Answer:

Explanationwe have two or three plants, they both get the same water every day they both get the same amount of soil and fertilizer, one is without sunlight and one is with, after a 2 weeks our results will be found

hope this helps

What fraction of the progeny of the cross BbTt x BbTt will have black fur and long tails?

A) 0/16

B) 1/16

C) 3/16

D) 9/16

E) 16/16

Answers

Answer:

plzzzz upload a full picture

what is 2 plus two and what is four plus four and what is five plus five and what is six plus six

Answers

Answer:

2+2=4. 4+4=8. 5+5=10. 6+6=12.

Explanation:

I'll just explain one? It takes around 360 pages to fully prove 1+1=2 so I'll just explain it the way I do. Draw some tallies. Draw 2 tallies and 2 more next to them. Count the total tallies, and you should get 4. (Sorry about this)

2+2 is 44+4 is 85+5 is 10 6+6 is 12

Explain how animals may have influenced the evolution of terrestrial plants and vice versa​

Answers

Answer:

Microsporangium give microspores by meiosis.

Microsporangium give microspores by meiosis each microspores forms a pollen grain Pollen wall is secreted by surrounding sporophytes tissue Enclosed male gametophyte produces sperm by mitosis.

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Anyone have a clue what dog breed this is?

Answers

Answer:

weenie dog but the scientific name for it is a Dotson

Its looks more like a beagle

What is NOT an effect of growth hormone?

a. Growth hormone directly stimulates osteoblasts in the periosteum to promote appositional growth.
b. Growth hormone increases the rate of mitosis in the epiphyseal plate of growing bones.
c. Growth hormone promotes the closure of the epiphyseal plate.
d. Growth hormone increases the activity of osteogenic cells.​

Answers

C. Growth hormone promotes the closure of the epiphyseal plate.

The closure of the epiphyseal plate is NOT an effect of growth hormone (option c).

The epiphyseal plate is a layer of hyaline cartilage between the epiphyses and metaphyses at the ends of long bones.

The epiphyseal plate is completely closed when bone growth is completed (around age 18 to 21 years).

Different hormones (i.e., testosterone and estrogen) released at puberty initiate the closure of epiphyseal plates.

In conclusion, the closure of the epiphyseal plate is NOT an effect of growth hormone (option c).

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Post Lab Report Part II 6. The probability that the two offspring you produced share every genotype in common is extremely low. Explain why they are likely to be different. 7. How would genetic variability be affected if more chromosome pairs were added? What about if another partner was added?

Answers

Answer:

Explanation:

Answer to 6:

The probability of two offspring with the same genotype is very low because due to recombination between the chromosomes of the father and mother each gamete has a different composition of genetic material. The only chance that they share the genotype is if they were zygotical twins.

Answer to question 7:

When you increase the number of chromosomes the variability will increase too.

And here is another one, help meeeee HUHUHU, I'm exhausted (only for those who know the answer)


I need explanation for your answers, even though it's multiple choices, I still need your explanation for it.

DUE TOMORROW!

If your answer is NONSENSE it will be deleted as soon as possible!

But if your answer is CORRECT, HELPFUL, HAS AN EXPLANATION, I'll chose your answer as the BRAINLIEST ANSWER!​

Answers

Answer:

14. (a) Pb

15. (d) Al

16. (A) increases

Explanation:

14.

In the reactivity series of metals an element can displace another element from a compound if and only if it is not placed below that metal, as the reactivity of metals decreases when moving from top to bottom- if a metal is placed below Hydrogen it means that it is less reactive than Hydrogen and hence cannot displace Hydrogen from its compound.

All options except A are placed below Hydrogen in the reactivity series, so they wont displace Hydrogen from its compound, but option (a) that is Pb can do so as it is above Hydrogen in the reactivity series.

15.

Al is placed below Mg in the reactivity series and hence cannot displace Mg from its compound.

16.

The reactivity of metals increases down the GROUP.

[CAUTION :- We were talking 'bout REACTIVITY SERIES in the top 2 questions where reactivity decreases down the group, but we're talkin' 'bout GROUPS of the periodic table here, where reactivity increases down the group.]

The size of metals increases down the group causing their valence electrons to drift far from the nucleus so that they can be lost easily. The metals have a property of losing electrons so the more electrons it can lose more will be its reactivity.

Hence, reactivity increases down the group.

who do study edexcell certificate level? and can help be physics,chemistry and biology exam​

Answers

I have done GCSE sciences and also applied science a level so I could probably help you :)

3. A bacterial isolate from a urine specimen was grown in culture, Gram stained, and then tested for its ability to ferment sugars and hydrolyze various subtrates. What approach to bacterial identification is this an example of

Answers

Answer:

Phenotypic approach for bacterial identification

Explanation:

Bacterial identification can be done by conventional methods, which are based on phenotypical characteristics. These methods are much affordable and reasonable.

Phenotypical identification is based on bacteria´s observable characteristics, such as their morphology, development, and biochemical/metabolic properties.

It is important to consider that these methods do not provide absolute certainty. They can only indicate the genera or species to which the bacteria under study may belong.

Some primary evidence is usually used for fast bacteria identification:

Gram staining, morphology, growth at different media or different incubation atmospheres, glucose fermentation, spores production, motion, aerobiosis/anaerobiosis, among others.  

Knowing that the bacteria in the exposed example was isolated and grown in culture, then Gram-stained and tested for biochemical reaction, we can assume that the approach for its identification is phenotypic.  

Which of the following statements about the law of conservation of matter is correct?
O Matter cannot be created, destroyed or transformed
O Matter can be created and destroyed, while still being transformed
O Matter cannot be created or destroyed, it can only be transformed

Answers

Answer:

3, Matter cannot be created or destroyed, only transformed

Answer:

matter cannot be created and or destroyed,it can only be transformed

Why do you think during the evolutionary process there was a correlation between brain size increasing while cecum size decreased in the omnivores?
How does insect respiration differ from that of vertebrate respiration? How do they manage to respire?

Answers

Answer:

Brain size increase due to find out improved quality diets whereas cecum size decrease due to reduction of its need.

Explanation:

Brain size increased due to the need to increase the mental capacity of an organism in order to explore the environment for the survival such as search for improved quality diet and the cecum size decreased because omnivorous diet improved due to the reduction of its need as a component of the digestive system. Insects have different respiration system as compared to vertebrates because vertebrates uses lungs for respiration whereas the insects uses tracheal tubes for the absorption of oxygen and removal of carbondioxide gas.

Define the term akaryotes and give one example ​

Answers

Answer:

These are cells without nucleus or nuclei.

Example: Red blood cells.

Lactase is an enzyme that is produced in the lining of the intestines. This enzyme helps the body speed up the
breakdown of carbohydrates that are found in dairy products, such as milk, yogurt, and cheese.
Which general function does lactase perform?

Answers

Answer:

Regulation of cell processes

Explanation:

Lactase is an enzyme that helps in the breaking down of disaccharide sugar, lactose, found in many foods. It does it by converting the lactose into its component reducing sugars - glucose and galactose. This enzyme is produced by some special cells lining the intestinal walls of humans and forms a kind of chemical barrier known as brush border where foods must pass through before being absorbed. By so doing, foods are screened for the presence of lactose and any lactose is broken down before the foods are given a pass.  

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Investment A cost 12,000 today and it pays back 15,500 two years from now. Investment B cost $8,000 today and it pays back $6,000 each year for two years. If Interest of 5% is used, which alternate is superior? (Hint: Use present worth your cost is negative and your profits are positive) Group of answer choices Graph the linear function described by the equation 1. All of the following statements are trueregarding spontaneous process, EXCEPT:A. It occurs without requiring an outsideforce.B. It can be a physical or chemical changeC. It will continue until equilibrium isreached.D. It will suddenly stop once products arformed.Which of the following is an example of aspontaneous process?A. Melting of ice in room temperatureB. Transfer of heat from a cold objectthot objectC. Removing rust on an iron nailD. Separation of hydrogen and oxygenatoms via electrolysis Which of these is the equation of the new function? Which of the following consists of discrete data?A. Number of suitcases on a plane.B. Amount of rainfall.C. Hair color.D. Tree height. A graphical representation of the earth, in three dimensional form, iscalled what?Select one:A globeA compassA mapA chart change the underlined verbs into past. l eat fruits , My teacher teaches well, The boys write nicely. Suppose Brian and Crystal are playing a game in which both must simultaneously choose the action Left or Right. The payoff matrix that follows shows the payoff each person will earn as a function of both of their choices. For example, the lower-right cell shows that if Brian chooses Right and Crystal chooses Right, Brian will receive a payoff of 5 and Crystal will receive a payoff of 6. Crystal Left Right Left 6, 3 6,4 Brian Right 3, 3 7,4 The only dominant strategy in this game is for_____to choose____. The outcome reflecting the unique Nash equilibrium in this game is as follows: Brian chooses____and Crystal chooses____. Which materials come together to form plaque? Select three options.salivamucusbacteriatoothpastefood particles Someone tell me where everyone is going right please !! What is your long term career goal? Select the correct statement(s) regarding network physical and logical topologies.a. While logical topologies can be configured in star, ring, bus, and tree configurations, the physical topology must always be in a full-mesh topologyb. logical topologies always incorporate centralized access, whereas physical topologies are always configured as a distributed access networkc. the physical topology addresses how devices are connected, while a logical topology addresses how devices actually communicate to one anotherd. all statements are correct Expenses that are not easily traced to a specific department, and which are incurred for the joint benefit of more than one department, are If you apply for a job with Globo Corp, there is a 75% chance that you will get an interview.If you get an interview, there is a 48% chance that you will get the job.If you apply for a job with Globo Corp, what is the probability that you will get the job?Express your answer as a decimal, rounded to the nearest hundredth. Pace Corporation has requested an estimate to rebuild its spot welder. Second Chance estimates that it would require 39 hours of labor and $2,800 of parts. Compute the total estimated bill what happens to gravitational force when distance is quarter from the originalplease answer it faster help me Which of the following statements describes one way that energy flows out of an ecosystem? In the following image, atoms are represented by colored circles. Different colors represent different types of atoms. Ifatoms are touching, they are bonded.Which of the following boxes shows a mixture of different compounds?ABCE The work of the three boys, does or do a great service Which of these is the best descriptionof fascism with regard to the power ofthe government?A. democratic power distributed among leadersB. socialist power administered by the peopleC. totalitarian power forcibly wielded by one leaderD. united power between a parliamentary body and amonarch