Answer:
boron (3 valence electrons = 3-valent) and phosphorus (5 valence electrons = 5-valent).
Which of the following complexes will absorb a photon with the lowest energy?
Group of answer choices
A. [Co(OH)6]3-
B. [Co(SCN)6]3-
C. [Co(NO2)6]3-
The coordination complex that will absorb a photon with the lowest energy is [Co(OH)₆]³⁻.
The complexes have their own absorption spectrum, and therefore they absorb photons of light at different energies, which are frequently shown as specific wavelengths.
The electronic configuration of the metal ions, the nature of the ligands, the geometry of the coordination compound, and the charge on the metal ion are all factors that affect the absorption spectrum of coordination compounds.
Therefore, the quantity of energy required for each complex to absorb a photon of light varies considerably.
Among the given complexes, the complex [Co(OH)₆]³⁻ has the lowest energy to absorb a photon. This is due to the fact that it has six bulky ligands of hydroxyl groups which are strong field ligands that cause crystal field splitting to be high.
As a result, the energy required to excite an electron from the lower energy d-orbitals to the higher energy d-orbitals would be lower. As a result, [Co(OH)₆]³⁻ will absorb a photon with the lowest energy than the other given complexes.
Therefore, the correct answer is option A.
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The epithelial cells of the proximal convoluted tubule are adapted for reabsorption by: a. having thin walls. b. having rugae folds that increase surface area. c. secreting enzymes that activate absorption of substances. d. having microscopic projections called microvilli.
Answer: d. possessing tiny protrusions known as microvilli. The proximal convoluted tubule's epithelial cells have microvilli on their surface, which increases their surface area and improves.
the efficiency of reabsorption of chemicals from the filtrate back into the circulation. The cells do not release enzymes to promote absorption, and the rugae folds and thin walls are not adaptations for reabsorption. The proximal convoluted tubule's epithelial cells are in charge of reabsorbing vital components from the glomerular filtrate, including glucose, amino acids, and electrolytes. Many microvilli, which are tiny finger-like projections on the surface of the cell that enhance the surface area accessible for reabsorption, are present in the cells, which enable them to perform this role. This modification makes it possible for chemicals to be reabsorbted more effectively as they move through the tubule. The rugae folds and thin walls, on the other hand, have little impact on reabsorption in the proximal convoluted tubule. In this region of the nephron, reabsorption likewise does not include enzyme secretion.
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what is the mass of potassium nitrate that will dissolve in 25g of water at 20° C?
a. 0.3g
b. 7.5g
c. 4g
d. 25g
e. 30g
Answer:
7.5g is the mass of potassium nitrate that will dissolve in 25g of water at 20° C?
The answer is 4g. At 20° C, the solubility of potassium nitrate in water is 3.7 g per 100 g of water, which means that about 4 g of potassium nitrate will dissolve in 25 g of water.
What is solubility?Solubility is the ability of a substance to dissolve in a liquid or other solvent. It is a measure of the maximum amount of substance that can be dissolved in a given amount of solvent. Solubility can be affected by factors such as temperature, pressure, and the type of solvent used. In general, the more soluble a substance is, the more easily it will dissolve. Solubility is an important factor in the formulation of many products, such as pharmaceuticals, cosmetics, and food products. It is also used in the manufacture of detergents, paints, and inks. Solubility can be determined experimentally, by measuring the amount of a substance that can be dissolved in a given volume of solvent. The solubility of a substance can also be calculated using equations that take into account the temperature and pressure of the system. Understanding solubility is important for determining the effectiveness of drugs, the shelf life of food products, and the environmental impact of certain chemicals.
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2) What is the volume of 8. 9 * 10 ^ 25 atoms of neon gas?
The volume of Neon gas is 326.5 L in 8. 9 * 10 ^ 25 atoms as per the ideal gas law equation.
The given atoms of Neon gas = 8. 9 * 10 ^ 25
To calculate the volume of any gas we should use the ideal gas law equation.
PV = nRT
Here R is the universal gas constant.
To calculate the volume, we need to calculate the number of moles of neon gas. Avogadro's number is used to convert the number of atoms to moles:
1 mole of neon gas = 6.022 * 10^23 atoms of neon gas
The total number of moles of neon gas is:
8.9 * 10^25 atoms / 6.022 * 10^23 atoms/mol = 14.79 mol
Let's assume the temperature is 25°C = 298 K
The pressure = 1 atm.
The value of R = 0.08206 Latm/(molK).
PV = nRT
V = (NRT) / P
V = (14.79 mol * 0.08206 Latm/(molK) * 298 K) / 1 atm
V = 326.5 L
Therefore we can conclude that the volume of Neon gas is 326.5 L.
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based on the chromatogram, which amino acids or substances were present in the hydrolyzed equal sample?
Answer: Based on the Chromatogram, the amino acids or substances present in the hydrolyzed equal sample are alanine, glycine, leucine, valine, isoleucine, and tyrosine.
Explanation:
Chromatogram is a graph or visual representation of the separated components of a mixture produced by chromatography. It provides information about the sample components, including their identity and relative amounts.
Based on the given chromatogram, Leucine, Tyrosine, and Phenylalanine amino acids or substances were present in the hydrolyzed equal sample. These amino acids are identified by their retention times, which can be compared to reference standards or databases to determine their identity.
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WILL GIVE BRAINLIEST!!!
Answer:
Select all that apply:
B
The number of reactant molecules and product molecules is even.
C and D
The reaction 2N2 + 3H2 → 2NH3 occurs.
Atoms can't be broken into smaller pieces and still retain the properties of that element. True or False?
true
Explanation:
Atoms cannot be subdivided, created, or destroyed. Atoms of different elements can combine in simple whole number ratios to form chemical compounds.
Answer:
true
hdozhsienzidbxirbfifndirhfirbr
If a neutral object lost 2 electrons, what would it's charge be? would it be positive?
If a neutral object lost two electrons, it would have a positive charge.
Electrons are negatively charged subatomic particles that orbit around the positively charged nucleus in an atom. When an atom loses electrons, it becomes positively charged because the total negative charge from the electrons is reduced. The number of electrons in an atom determines its overall charge, and when electrons are lost, the atom's charge becomes more positive.
In this case, the neutral object would have a net positive charge equal to the number of protons in its nucleus, since it has lost two negatively charged electrons. Thus, the object would be positively charged after losing two electrons.
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Look at the transition you drew for Element Y on the earlier slide. On a piece of paper, write out your calculation to find the energy of the photon emitted. Calculate the wavelength (in nm) of the photon emitted for Element Y. Calculate the frequency of the photon emitted for Element Y.
To calculate the energy of the photon emitted for Element Y, you can use the following equation:
E = hc/λ
where h is Planck's constant (6.626 x 10-34 Js), c is the speed of light (3 x 108 m/s), and λ is the wavelength of the photon (in meters).
To calculate the wavelength of the photon emitted for Element Y, you can use the following equation:
λ = hc/E
where h is Planck's constant (6.626 x 10-34 Js), c is the speed of light (3 x 108 m/s), and E is the energy of the photon (in Joules)
To calculate the frequency of the photon emitted for Element Y, you can use the following equation:
f = c/λ
where c is the speed of light (3 x 108 m/s), and λ is the wavelength of the photon (in meters).
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ksp for calcium carbonate is 3.0 × 10–9. if you mix together 100 ml of 0.010 m calcium chloride with 100 ml of 1.0 × 10–5 m solution of potassium carbonate, will a precipitate form?
Yes, a precipitate will form if you mix together 100 mL of 0.010 m calcium chloride with 100 mL of 1.0 x 10-5 m solution of potassium carbonate.
This is because the Ksp (solubility product constant) for calcium carbonate is 3.0 x 10-9. This means that when the concentrations of calcium and carbonate ions become too high, they will no longer be soluble and a precipitate will form. To understand this better, consider the following reaction:
CaCl2 (aq) + CO3 (aq) --> CaCO3 (s) + 2Cl- (aq).
This equation shows that calcium ions (Ca2+) from the calcium chloride solution will react with carbonate ions (CO3-) from the potassium carbonate solution to form solid calcium carbonate (CaCO3) and chloride ions (Cl-). When the concentrations of calcium and carbonate ions in the solution become too high, a precipitate will form as calcium carbonate.
The Ksp for calcium carbonate is a measure of how much calcium and carbonate ions can be present in the solution before a precipitate forms. This means that if the concentrations of the calcium and carbonate ions become greater than 3.0 x 10-9 molar, a precipitate will form. The concentrations of the calcium and carbonate ions in the given solutions are 0.010 m and 1.0 x 10-5 m respectively, both of which are greater than the Ksp of calcium carbonate and hence a precipitate will form.
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total no. of atoms in 90 g of water
Answer:
Step 1: Determine the molecular weight of water. The molecular weight of water is 18.015 g/mol.
Step 2: Calculate the number of moles. To calculate the number of moles, divide 90g by the molecular weight of water to get 5 moles of water.
Step 3: Calculate the number of atoms of water present. One mole of water contains 6.022 x 10^23 molecules of water. Therefore, 5 moles of water contains (5 x 6.022 x 10^23) = 3.011 x 10^24 atoms of water.
Therefore, there are 3.011 x 10^24 atoms of water present in 90g of water.
Which type of reaction will occur when equal volumes of 0.1 M HCl and 0.1 M NaOH are mixed 1. Neutralization2. Ionization3. Electrolysis4. Hydrolysis
When 0.1 M HCl and 0.1 M NaOH are combined in equal amounts, a process known as neutralisation will take place, producing water and a salt as byproducts.
In a neutralisation process, which takes place when an acid and a base are mixed, the H+ ions from the acid and the OH- ions from the base combine to generate water molecules. In this instance, when 0.1 M HCl and 0.1 M NaOH are combined in equal parts, they will react 1:1 to form water and the salt NaCl. The final mixture's pH will be 7, which is neutral. This is due to the fact that only water and salt are left after the reaction, totally consuming the H+ and OH- ions. A lot of industries, including industry, agriculture, and medicine, utilise this kind of reaction.
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Which of the following indicates a spontaneous reaction under standard conditions? A) K = 8.6 x 10⁻². B) K = 7.9 x 10⁻⁸. C) K = 2.2 x 10².
A spontaneous reaction under standard conditions is indicated by the value of K being greater than 1. Thus, the answer to the given question is option C, K = 2.2 x 10².
Standard conditions- Standard conditions are a set of environmental conditions that are considered to be the standard conditions for conducting an experiment. They serve as a reference point to compare the effects of varying environmental conditions on the properties of a substance or the results of an experiment.
Standard conditions in chemistry are considered to be a temperature of 298K (25°C), a pressure of 1 atm (101.3 kPa), and a concentration of 1 mol/L (for solutions).
Spontaneous reaction- A spontaneous reaction is one that proceeds without any external force or intervention. That is, a spontaneous reaction proceeds without the need for energy input from an external source. In other words, it is an exothermic reaction where the products are more stable than the reactants.
The Gibbs free energy change of a spontaneous reaction is negative. The sign of ΔG indicates the spontaneity of a reaction. A negative value indicates that the reaction is spontaneous, whereas a positive value indicates that the reaction is non-spontaneous. The value of ΔG° is used to determine the spontaneity of a reaction under standard conditions.
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one difference between first- and second-order reactions is that ____ . a) whereas the half-life of a second-order reaction does depend on it. the rate of a first-order reaction does not depend on reactant concentrations whereas b) th e rate of a second-order reaction does depend on reactant concentrations. the half-life of a first-order reaction depends on the initial concentration whereas the half-life of a second-order reaction does not depend on it. c) the rate of a first-order reaction depends on reactant concentrations whereas the rate of a second-order reaction does not depend on reactant concentrations
The correct option is C .The difference between first- and second-order reactions is that the rate of a first-order reaction depends on reactant concentrations, whereas the rate of a second-order reaction does not depend on reactant concentrations.
The order of a reaction is determined by the power to which the concentration of each reactant is raised in the rate law. A first-order reaction is a chemical reaction in which the rate of reaction is proportional to the concentration of only one reactant (unimolecular reaction), and the rate equation can be expressed in terms of the concentration of the reactant as d[A] /dt = - k[A], where [A] is the concentration of the reactant and k is the rate constant. Second-order reactions are chemical reactions in which the rate of reaction is proportional to the square of the concentration of one reactant, or proportional to the product of the concentrations of two reactants (bimolecular reaction).
However, The rate of a first-order reaction does not depend on the initial concentration of the reactant, whereas the half-life of a first-order reaction depends on the initial concentration. On the other hand, the rate of a second-order reaction does depend on reactant concentrations, whereas the half-life of a second-order reaction does not depend on the initial concentration.
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Determine the overall reaction and its standard cell potential at 25 �C for the reaction involving the galvanic cell made from a half-cell consisting of a silver electrode in 1 M silver nitrate solution and a half-cell consisting of a zinc electrode in 1 M zinc nitrate. Is the reaction spontaneous at standard conditions?
The reaction involved in the galvanic cell made from a half-cell consisting of a silver electrode in 1 M silver nitrate solution and a half-cell consisting of a zinc electrode in 1 M zinc nitrate is given as follows:2 Ag(s) + Zn2+ (aq) → Zn(s) + 2 Ag+ (aq)The standard cell potential at 25 °C for the given reaction can be determined using the following formula: E°cell
= E°cathode - E°anodeHere, the E°cathode and E°anode represent the standard reduction potentials of cathode and anode respectively. The values of these standard reduction potentials can be obtained from the standard reduction
potentials table.Using the values of standard reduction potentials from the table, we have:E°cell = E°Ag+ / Ag - E°Zn2+ / Zn= +0.80 V - (-0.76 V)= +1.56 VThe reaction is spontaneous at standard conditions because the calculated standard
cell potential is positive (+1.56 V). Therefore, the reaction will proceed spontaneously from left to right direction.The bolded non-consecutive keywords are: spontaneous, standard conditions, galvanic cell, reduction potentials.
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Please use the Atomic Spectroscopy interactive to answer the question. Rank the strength of the given hydrogen emission lines from weakest to strongest.
The hydrogen emission lines in decreasing order of strength are as follows :Balmer α, Balmer β, Balmer γ, Balmer δ and Balmer ε. At a lower energy level, hydrogen is excited by heating or a spark, causing its electrons to jump to higher energy levels.
These electrons subsequently emit photons in the visible, ultraviolet, and infrared regions of the electromagnetic spectrum when they drop back to their lower energy levels.Atomic spectroscopy is a spectroscopic method that examines the spectrum of an atom in the gas phase by absorbing or emitting radiation.
It is a method of elemental analysis that is widely used in the laboratory and industry to determine the presence and concentration of trace elements in a sample. Atomic spectroscopy is divided into three categories: atomic absorption, atomic emission, and atomic fluorescence. All three techniques involve irradiating a sample with radiation of a specific frequency, resulting in the excitation or ionization of atoms or ions. The difference between the techniques is how the sample's interaction with the radiation is measured.
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Which catastrophic events can be started due to an earthquake? Select all that apply.
A- landslide
B- tsunami
C- tornado
D- hurricane
Answer: A and B
Explanation:
i took the test hope it will help --_o
which type of radioactive emission is considered the most dangerous?
Gamma radiation are the most dangerous type of radioactive emission as they are the most energetic and can penetrate the human body and damage cells. Alpha particles can cause both short-term and long-term health effects, such as cancer.
Out of alpha, beta and gamma radiation, the most dangerous type of radioactive emission is gamma radiation. This is because gamma rays are penetrating, high-energy rays that can easily penetrate the human body and cause damage to cells and DNA. Gamma radiation, unlike alpha and beta radiation, can penetrate the body's skin and tissue, exposing internal organs to radiation. When gamma rays are absorbed by living cells, they can ionize atoms and molecules, causing damage to DNA and other genetic material in the cell. High doses of gamma radiation can cause immediate symptoms such as radiation sickness and even death. Gamma radiation is frequently emitted by unstable radioactive atoms like uranium and plutonium, which are used in nuclear power plants and nuclear weapons. Workers in these industries and anyone exposed to a nuclear accident or bomb are at a higher risk of exposure to gamma radiation. Alpha radiation is a type of ionizing radiation that is emitted by certain types of unstable atoms. Alpha particles are relatively large and have a short range, so they can be stopped by a sheet of paper or the outer layer of human skin. Beta radiation is a type of ionizing radiation that is emitted by certain types of unstable atoms. Beta particles are much smaller than alpha particles, and they can travel through the human body farther than alpha particles. Gamma radiation is a type of electromagnetic radiation, similar to X-rays, but with higher energy and frequency. Gamma rays are produced by the decay of unstable atomic nuclei and are highly penetrating, meaning they can easily pass through solid objects.
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If a flexible hot air balloon has a volume of 350,000 L of air at 40 degrees C and 1 atm,
how many moles of air are in the balloon?
How do I solve this?
If a flexible hot air balloon has a volume of 350,000 L of air at 40 degrees C and 1 atm, 13636 moles of air are in the balloon . This is taken out by ideal gas law.
What is ideal gas law?The ideal gas law, also known as the general gas equation, is the state equation for a hypothetical ideal gas. Although it has several limitations, it is a good approximation of the behavior of many gases under many conditions. Benoît Paul Émile Clapeyron proposed it in 1834 as a combination of empirical Boyle's law, Charles' law, Avogadro's law, and Gay Lussac's law.
using the equation P × V= n × R × T
P = Pressure = 1 atm
V = Volume = 350000 L
n = moles
R = 0.082 L.atm.K⁻¹.mol⁻¹
solving for n ,
moles n = 13636 moles
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What is the apparent brightness of a star?
I suppose how dazzling the star appear to a meter here on Earth. On the opposing hand, a star's brightness refers to how much light is emitted from its surface.
What is a star's apparent and absolute brightness?absolute magnitude: the amount of brightness a star would have at a given distance. The brightness of a galaxy seen from the Earth is known as its apparent magnitude.
What are apparent and actual brightness?Because of this, the sample's name, "apparent" brightness, refers to the brightness of things as seen from our vantage point rather than their actual or "true" brightness. Astronomers must measure a star's luminosity, or absolute brightness, in to understand the precise amount of heat it emits.
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based on what you understand about the density of co2, what do you suspect happened to the normal air after the co2 was released from the lake? group of answer choices the normal air was displaced by the heavier co2 the co2 was displaced by the heavier normal air the normal air stayed where it was originally
After the CO₂ was released from the lake, the normal air was displaced by the heavier CO₂. This option is correct.
CO₂, or carbon dioxide, is a gas that is slightly heavier than air. The density of CO₂ is approximately 1.98 kg/m³, whereas the density of air is around 1.2 kg/m³.
This means that CO₂ is denser than air and has a greater mass per unit volume. When CO₂ is released from a lake, it will displace the normal air in the surrounding area because of its higher density.
The normal air stayed where it was originally, and it did not displace the heavier CO₂ since CO₂ is heavier than air.
So, the correct option is "the normal air was displaced by the heavier CO₂ ."
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In an open manometer with an atmospheric pressure of 104 kPa, the mercury level in the arm connected to the gas is 150 mm Hg lower than in the arm connected to the atmosphere. What is the pressure of the gas sample?
A triglyceride that loses a fatty acid is called a ____.
A triglyceride that loses a fatty acid is called a diglyceride or diacylglycerol. This occurs when the triglyceride molecule undergoes a process called hydrolysis.
Hydrolysis breaks the ester bond between one of the fatty acids and the glycerol backbone, releasing a free fatty acid and forming a diglyceride.
This process is essential in the metabolism and digestion of fats in our bodies, as it allows the body to break down complex fat molecules into simpler components that can be absorbed and utilized for energy or other biological functions.
In addition, diglycerides play important roles in cell signaling and membrane structure, serving as essential building blocks for various cellular components.
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How can scientists
use ionization
energy to learn more
about elements that
have not been
discovered yet?
The ionization energy, or ionization potential, is the energy required to completely remove an electron from a gaseous atom or ion. The closer and more tightly bound an electron is to the nucleus, the more difficult it will be to remove, and the higher its ionization energy will be.
Select all of the following molecules that contain stereocenters.-alkene with H wedges and CH3 dash-both 1,2-dimethylcyclohexane-cyclohexane with wedge-dash methyl
The stereocenter-containing molecule is:
1,2-dimethylcyclohexane (both carbons at position 1 and 2 are stereocenters as they have four distinct substituents connected to them)
(both carbons at position 1 and 2 are stereocenters as they have four different substituents attached to them)
Stereocenters are absent from the other compounds that were listed. Because the double bond does not include any substituents that are distinct from one another, the alkene with H wedges and the CH3 dash does not have stereocenters. As all of the carbons in cyclohexane are symmetric and do not have four separate substituents connected to them, the cyclohexane with wedge-dash methyl likewise lacks stereocenters., the only molecule in the list that contains stereocenters is 1,2-dimethylcyclohexane.
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A balloon has a volume of 800.0 mL on a day when the temperature is 308 K. If the temperature at night falls to 263 K, what will be the volume of the balloon?
The volume of the balloon at a temperature of 263 K will be approximately 683.1 mL.
What will be the volume of the balloon?Charles's Law states that the volume of a gas is directly proportional to its absolute temperature at constant pressure.
This means that the volume and temperature of a gas are directly proportional to each other as long as the pressure is constant.
It is expressed as:
V₁/T₁ = V₂/T₂
Where V₁ and T₁ are the initial volume and temperature, V₂ is the final volume, and T₂ is the final temperature.
Given that:
V₁ = 800.0 mLT₁ = 308 KT₂ = 263 KSolving for V₂, we get:
V₂ = V₁T₂ / T₁
V₂ = ( 800 × 263 ) / 308
V₂ = 210400 / 308
V₂ = 683.1 mL
Therefore, the volume is 683.1 mL.
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An electrochemical cell consists of the half-cells Cu2+Cu and Ag IAg. a. Write half-reactions for the reduction of Cu2+ to Cu and Ag to Ag. Include the standard reduction potentials, & b. Write the half-reactions that occur at the anode and at the cathode. Label these. c. Write the balanced equation for this reaction d. How many electrons are transferred during this reaction? e. Write the reaction quotient
a. Ther half-reactions and standard reduction potentials for the reduction of Cu2+ to Cu and Ag to Ag are as follows:-
Cu2+ + 2 e– → Cu, E° = 0.34 V
Ag+ + e– → Ag, E° = 0.80 V
b. The half-reaction at the anode: Cu → Cu2+ + 2 e– (oxidation)
The half-reaction at the cathode: Ag+ + e– → Ag (reduction)
c. Balanced equation for this reaction is as follows:-
Cu + 2 Ag+ → Cu2+ + 2 Ag
d. Two electrons are transferred during this reaction.
e. The reaction quotient (Q) is determined using the equation: Q = [Cu2+][Ag]2 / [Cu][Ag+]2
The anode is the site of oxidation, and the cathode is the site of reduction.
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Part A Inhibition of which of the following metabolic pathways would result in decreased rates of CO2 production? O fermentation and glycolysis O oxidation of pyruvate to acetyl CoA and the citric acid cycle glycolysis and the oxidation of pyruvate to acetyl COA O oxidative phosphorylation and fermentation Submit Request Answer Provide Feedback hapter 10 apter 10 Question 11 12 of Part A A mutation that disrupts cyclic electron flow in the light reactions of photosynthesis will specifically reduce the production of which of the following molecules? O NADPH O CO2 O ATP ADP and NADP Submit Recuest Answer Provide Feedback Part A Which of the following statements best summarizes the metabolic results of photorespiration? O ATP is hydrolyzed, oxygen is produced, and carbon dioxide is consumed. O ATP is hydrolyzed, carbon dioxide is produced, and oxygen is consumed. O ATP is produced, oxygen and carbon dioxide are consumed. O ATP and oxygen are produced and carbon dioxide is consumed. Submit Request Answer < Return to Assignment Provide Feedback
Photorespiration is a process which occurs in plants when there is not enough CO2 available for photosynthesis, resulting in the hydrolysis of ATP.
During photorespiration, oxygen is consumed and carbon dioxide is produced.
The light reactions of photosynthesis produce NADPH and ATP, and when cyclic electron flow is disrupted due to a mutation, the production of NADPH will be reduced.
Without NADPH, the Calvin cycle will not proceed, resulting in the production of glycolic acid, which is further broken down to form glycine, ammonia and carbon dioxide.
This process results in the hydrolysis of ATP and the consumption of oxygen, with the production of carbon dioxide as a by-product.
Photorespiration results in the hydrolysis of ATP, the consumption of oxygen and the production of carbon dioxide.
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Why were dirigibles considered to be the wave of the future in travel?
Answer:
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A culture of E. coli growing in a medium containing 15NH4Cl15NH4Cl is switched to a medium containing 14NH4Cl14NH4Cl for three generations (an eightfold increase in population). What is the molar ratio of hybrid DNA ( 15N14−N15N−14N ) to light DNA ( 14N14−N14N−14N ) at this point?
The switch from ¹⁵NH₄Cl to ¹⁴NH₄Cl for three generations (an eightfold increase in population) in a culture of E. coli will result in a molar ratio of hybrid DNA ( ¹⁵N-¹⁴N) to light DNA (¹⁴N-¹⁴N) of 3:1.
The reason for this is that during the first replication cycle in the ¹⁴NH₄Cl-containing medium, the hybrid DNA strands will split, and half of the daughter DNA molecules will contain a heavy ¹⁵N nitrogen atom and a light ¹⁴N nitrogen atom.
The remaining daughter DNA molecules will have two light ¹⁴N nitrogen atoms, resulting in a molar ratio of 1:1.
During the next replication cycle, the hybrid DNA strands, in the light ¹⁴NH₄Cl-containing medium, will split once again, and the resulting daughter DNA molecules will either have one heavy and one light nitrogen atom or two light nitrogen atoms. Thus there will be 2 hybrid DNA and 2 pure DNA with light nitrogen, resulting in the same molar ratio of 1:1.
In the next cycle, the 2 molecules with light nitrogen give 4 pure molecules and 2 more pure molecules come from the strand of each hybrid molecule. Thus there is a total of 6 pure molecules. The heavy nitrogen strand of the hybrids gives rise to 2 more hybrids. So, there is a total of 2 hybrids and 6 light nitrogen molecules, resulting in a molar ratio of 3:2.
Therefore, the molar ratio of hybrid DNA ( ¹⁵N-¹⁴N) to light DNA (¹⁴N-¹⁴N) of 3:1.
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