What are sizes of the fragments from an enzyme digestions (endonuclease) of the Lambda Phage plasmid DNA using Eco RI and Pst I? Make sure to have an uncut and double-cut reaction. Explain the mechanism of action of each enzyme and then list the fragment all sizes and draw the results.

Answers

Answer 1

Digestion of lambda DNA with Eco RI generated 6 fragments. The Pst I restriction enzyme produces 29 lambda DNA fragments.

What are restriction enzymes?Restriction enzymes are DNA-cutting enzymes found in bacteria. These are site-specific endonucleases also known as molecular scissors.These recognize and cut DNA only at a particular sequence of nucleotides.DNA from the bacteriophage Lambda (48,502 base pairs in length) is cut with a variety of restriction enzymes and the resulting fragments are separated using gel electrophoresis. Size of fragment of uncut lambda DNA is 48502 b.p.

Eco RI recognizes G AATTC

                               CTTAA G  

Lambda phage has 5 recogition sites for Eco RI. It cleaves the DNA at ‘G’ of the above sequence.Relative sites of cleavage in base pairs are 21226, 26104, 31747, 39168, 44972.Sizes of fragments produced when the lambda DNA cut with Eco RI 21226, 7421, 5804, 5643, 4878, 3530.Digestion of lambda DNA with EcoRI generated 6 fragments but 2 of them are so close in size that they are difficult to separate.

Pst I recognizes CTGCA G

                           G ACGTC

The Pst I restriction enzyme produces 29 fragments of lambda DNA. It cleaves the DNA at ‘G’ of the above sequence.The Pst I digest of λ DNA yields the following discrete fragments (in base pairs): 11497, 5077, 4749, 4507, 2838, 2556, 2459, 2443, 2140, 1986, 1700, 1159 1093, 805, 514, 468, 448, 339, 264, 247, 216, 211, 200, 164, 150, 94, 87, 72 and 15.The cohesive ends (12b cos site of bacteriophage) of fragments 11501 bp and 2556 bp may anneal and form an additional band.

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Related Questions

True or False

a. The sinus node generates an electrical impulse.

b. The bundle of His transfers the impulse to the AV node.

c. The fiber group stimulates only the right ventricles.

d. The cardiac cycle represents one complete heartbeat.

e. Relaxation events are referred to as diastole.

Answers

Answer:

Explanation:

A-true

B-false

C-false

D-true

E- true

How do our social groups and social interactions impact our behavior?

Answers

Answer:

it makes us addict to fame and people would do anything to be higher up in the social groups

Answer:

When we interact with others, the context in which our actions take place plays a major role in our behavior. This means that our understanding of objects, words, emotions, and social cues may differ depending on where we encounter them

Explanation: Enjoy your day!

7. A man sitting outside a restaurant on a park bench is smoking a cigarette while reading a newspaper. Which answer best describes the mutagen to which the man is exposed?
O The newspaper
The cigarette
The oxygen he is breathing
The park bench​

Answers

the cigarette, tobacco is a mutagen because of the affect

2 differences between
monocotyledondus and dicotyledonas plants

Answers

Answer:

1)The monocot embryos have a single cotyledon.

The dicot embryos have a pair of cotyledons.

monocotyledons

2)They have a fibrous root system.

dicotyledonous.

They have a tap root system.

discuss how classification system have undergone​

Answers

Answer:

The classification systems have undergone several changes with time. The first attempt of classification was made by Aristotle. He classified plants as herbs, shrubs, and trees. Animals, on the other hand, were classified on the basis of presence or absence of red blood cells.

I hope it's helpful!

process of gathering information about events or processes in an orderly way

Answers

Answer:

Observation is the process of gathering information about events or processes in an orderly way

Which configuration and conditions helped the perfume molecules diffuse better?​

Answers

Answer:

Higher temperatures increase the energy and therefore the movement of the molecules, increasing the rate of diffusion

Explanation:

As the density of a solvent increases, the rate of diffusion decreases. yep i know

What is a simple diffusion?

Answers

Answer:

movement of a solute from an area of high concentration to an area of low concentration

Explanation:

2. Codominance: Diricrawls are plump, fluffy-feathered, flightless birds. Feather color
is co-dominant; FF have pink feathers, PP have purple feathers and mottled diriclaws (FP)
have both pink and purple feathers. Pink feathered diricrawls are very rare and prized by
collectors. If you breed a pink diriclaw with a mottled diricrawls, what percentage of their
offspring will be pink feathered? (show all of your work and use a Punnett square).

Answers

Answer:

The correct answer is - 50% or 1/2.

Explanation:

In codominance both alleles express their traits and blend to make new traits, there is no dominance or recessive pattern found. In this question, there is FF have pink feathers, PP has purple feathers and mottled diriclaws (FP). Then a cross between pink feathers and mottled diriclaws would produce offspring:

    F     F

F  FF  FF

P  FP  FP

Here two out of four have pink feathers. That is 50%

Which of the following is a macronutrient?
A) iron B) boron C) Calcium D) Manganese

Answers

C. calcium is your answer

why can it take soil thousands of years to form

Answers

A: We say that it takes 500 to thousands of years to create an inch of topsoil. The reason is that soil is often derived from rock. The rock has to be broken into small pieces first. This happens by physical weathering: things like freezing and thawing in colder climates, and chemical weathering in warmer climates.

what would probably happen if demario shared a drinking glass with his best friend who was sick with the flu and had a fever

Answers

Answer:

Demario would probably get the flu as well

Explanation:

Demario would become sick with the flu within a few days.

Which gland is located closest to the human brain?
A The thyroid
B The pituitary
C The adrenal
D The ovaries

Answers

Answer:

B. The pituitary gland

Explanation:

Can't be the ovaries, they're down by the hips!

Can't be adrenal glands, they're on top of the kidneys!

Can't be the thyroid, that's low on the front of the neck or something like that!

It's B, the pituitary; it's on the base of the brain.

Have a great day!

Are physical characteristics of an organism not genetically passed down?

Answers

That is false. Physical characteristics of an organism are genetically passed down.

—Evidence—

A characteristic that an organism can pass on to its offspring through its genes.

The set of information that controls a trait; a segment of DNA on a chromosone that codes for a specific trait.

Heredity is the biological process responsible for passing on physical traits from one generation to another.

Type the correct answer in the box. Spell all words correctly.
Which gas prevents the spread of fire and extinguishes it?
Blank. is a type of compressed gas that prevents the spread of fire and extinguishes it.
Help ASAP !!!

Answers

Sodium Bicarbonate which releases a cloud of Carbon dioxide gas which can prevent the spread of fire and extinguish it

Answer:

Halon

Explanation:

I got it correct on Plato

How do environmental issues impact food production?

Answers

Answer:

hola esta en ingles alguien sabe como ponerlo em español

What peppery of water allows it to move up through a plant?

Answers

Its A, hydrogen bonding

Answer:

A. Hydrogen Bonds

Can someone please help me please

Answers

Answer:

I think so answer is D

Explanation:

I hope it helps you

Pl Mark brainlist answer

Mitosis and budding are similar beu
O
Both processes are components of sexual reproduction
The offspring produced by both are genetically diverse
In both, the genetic material comes from a single parent.
O O
Both processes are more common in animals than in plants.

Answers

Answer:

animals would be your awnser you are welcome  

Explanation:

keep it cool ansd answer my question if u can! :))

Answers

Hind legs, D. Hope this helps!

design your own, you can use the Browse feature to attach and upload your document. 5. Design your own experiment based on one of your questions from question 1, above. Include the question, hypothesis, independent and dependent variables, and safety precautions. 6. What are three major branches of Earth science?​

Answers

Answer:

three major branches of earth science are Geology, oceanography, meteorology.

Which of the following statements is NOT part of the cell theory? a All cells originate from other cells. b The most basic component of any organism is the cell. c All living things are made up of one or more cells. d All cells have a nucleus and a cell membrane.

Answers

Answer:

d) All cells have a nucleus and a cell membrane.

Explanation:

Prokaryotes do not have a nucleus

All cells have nucleus and cell membrane


Where would you least
likely find a new
volcano?
A. Mid-ocean ridge
B. "Hot spot" in Hawaii
C. The great plains in the middle
of the U.S.
D. Near a subduction zone in
Argentina
Secon2015
I NEED TO KNOW ASAP

Answers

Answer:

A. Mid-ocean ridge. I'm not sure if I'm right.

The place where the new volcanoes are less likely to be seen is mid-ocean ridge. The correct option is A.

What is volcano?

A volcano is a crack in the crust of a planet, such as Earth, that allows hot lava, volcanic ash, and gases to escape from a magma chamber beneath the surface.

Volcanoes are most commonly found where tectonic plates are diverging or converging on Earth, and the majority are found underwater.

Although Australia is the only continent with no active volcanoes, it does have one of the world's largest extinct volcanoes, the Tweed Volcano.

Volcanism is directly related to plate tectonics theory. Volcanoes are only found at specific types of plate boundaries, and only rarely in the middle of ocean plates.

Thus, the correct option is A.

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____is a restriction to the flow of electrons

Answers

Answer:

Impedance.

Explanation:

Conductors allows the flow of electrons and Impedance restricts it.

PLEASE HELP!!!!! I NEED PERSON GOOD WITH AP BIOLOGY!! I WILL GIVE BRAINLIEST PLS!!!

Answers

Answer:

if crossing over does not occur, the products are parental gametes. If crossing over occurs, the products are recombinant gametes. Meaning they are mixed.

Explanation:

If crossing over doesn't occurs then you wouldn't really mix the 2 parental alleles so you couldn't have a genotype without mixing.

Sorry if im wrong!

PLS HELP ILL MARK BRAILIEST
Spend one minute writing an explanation of
the FUNCTION of enzymes. YOU MUST USE
THE WORDS CATALYST, ACTIVATION
ENERGY, RECYCLABLE

Answers

Like all catalysts, enzymes work by lowering the activation energy of chemical reactions. Activation energy is the energy needed to start a chemical reaction. This is illustrated in Figure below. The biochemical reaction shown in the figure requires about three times as much activation energy without the enzyme as it does with the enzyme.

The reaction represented by this graph is a combustion reaction involving the reactants glucose (C6H12O6) and oxygen (O2). The products of the reaction are carbon dioxide (CO2) and water (H2O). Energy is also released during the reaction. The enzyme speeds up the reaction by lowering the activation energy needed for the reaction to start. Compare the activation energy with and without the enzyme

Answer:

Enzymes help speed up chemical reactions in the human body. They bind to molecules and alter them in specific ways. They are essential for respiration, digesting food, muscle and nerve function, among thousands of other roles.

What is the function of an enzyme? They allow chemical reactions to occur at normal body temperature fast enough to sustain life. They reduce the activation energy needed to start a chemical reaction.

There are four steps in the process of an enzyme working. (1) An enzyme and a SUBSTRATE are in the same area. The substrate is the biological molecule that the enzyme will work on. (2) The enzyme grabs onto the substrate with a special area called the ACTIVE SITE.

There are four steps in the process of an enzyme working. (1) An enzyme and a SUBSTRATE are in the same area. The substrate is the biological molecule that the enzyme will work on. (2) The enzyme grabs onto the substrate with a special area called the ACTIVE SITE.

Enzymes create chemical reactions in the body. They actually speed up the rate of a chemical reaction to help support life. The enzymes in your body help to perform very important tasks. These include building muscle, destroying toxins, and breaking down food particles during digestion.

ILL GIVE YOU BRAINLYIST

Answers

Answer:

d

D

D

D

D

D

Explanation:

DD

D

D

D

D

D

A mouse are 380 calories worth of cherries. A fox catches it . How many calories does the fox obtain?

Answers

Answer:

The correct answer would be - 38 calories.

Explanation:

Subduction zones occur along
a. divergent boundaries.
b. convergent boundaries.
c. transform boundaries.
d. in the middle of plates, above hot spots.

Answers

Answer:

B, porque es la mejor respuesta de la pregunta

If the sphincter allows any leakage from the stomach, some of this acid might move back into the esophagus, causing
1. Heartburn
2. Saliva
3. Constipation
4. Diarrhea

Answers

1, heartburn. Keep stomach acid in the stomach!
Heartburn bc of the acid In your stomach
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