The Gram stain provides a lot of clinically useful information but it won’t give you all the information you need for identification and treatment. About which of these do you NOT learn anything from the Gram stain?

Answers

Answer 1

Answer: d) The ability of the bacteria to process nutrients.

Explanation:

Incomplete question.

Options,

a) Structure of the cell wall b) Bacterial morphology c) Susceptibility to antimicrobial drugs d) The ability of the bacteria to process nutrients.

Gram staining is a type of differential staining to study bacteria, and is used to see cell morphology and to differentiate between gram-positive bacteria that appear violet and gram-negative bacteria that appear pink or red.

To perform the staining, fix the samples with methanol for one minute or heat and add a dye called crystal violet and wait one minute. Then rinse with water and add lugol for one minute. Then add acetone alcohol for 5-30 seconds. At this point, gram-negative bacteria discolor while gram-positive bacteria do not. After this, a contrast staining is performed by adding safranin or basic fuchsin for one minute. This stain will turn the gram-negative bacteria pinkish-reddish.

Crystal violet is a cationic dye that penetrates all bacteria through the bacterial wall. Lugol is a compound consisting of iodine and potassium iodide and acts as a mordant. The iodine enters the bacteria and forms an insoluble complex with the crystal violet, causing the crystal violet to bind more strongly to the bacterial cell wall. The added alcohol and acetone are used for decolorization, since the iodine/violet crystal complex is soluble in the alcohol and acetone. Thus, gram-positive bacteria do not decolorize, while gram-negative bacteria do.

A contrast stain such as safranin or fuchsin is used to highlight gram-negative cells. After contrast staining, gram-negative cells are pink or red, while gram-positive cells remain purple.

The wall of gram-positive bacteria consists of about forty layers of peptidoglycan which does not allow alcohol to pass through it because it is very thick, while that of gram-negative bacteria consists of a single layer of peptidoglycan. These differences cause gram-positive bacteria to retain the violet color and gram-negative bacteria to discolor.

So, with this type of staining we can certainly see the structure of the cell wall, because those that retain the violet dye even after treatment with a decolorant are gram-positive (many layers of peptodoglycan) and those that easily lose the first dye and take up the second are gram-negative (single layer). It also gives us an idea of the shape and morphology, which when stained will be visible under the microscope.

It also informs us about susceptibility to antibiotics, since some act only on gram-positive bacteria and others only on gram-negative bacteria. For example, gram-positive bacteria are sensitive to beta lactamase/penicillinase and gram-negative bacteria are susceptible to broad-spectrum penicillins such as piperacillin. However, this type of staining will not tell us about the bacteria's ability to process nutrients, because that is not a process related to the structure of the cell wall.


Related Questions

why is climate change a serious threat to the least developed countries practicing agriculture as their main occupation mountain region island and low lying countries​

Answers

Answer:

Increase in sea level on the low lying countries which leads to degradation and salinification thereby making the soil non-arable. Meanwhile on the mountain regions, melting glaciers caused by rain falling on snow leads to mountain floods, avalanches and extreme rockslide and thereby disrupting agricultural produce and wildlife.

Explanation:

An adult giraffe (Giraffa camelopardalis) has a heart that lies approximately 2 m below the top of the head when its neck is fully extended. Calculate the minimum diastolic pressure that a giraffe must have (in mm Hg). Hint: what is the absolute minimum blood pressure a giraffe could have at the very top of its head

Answers

Answer:

159.01 mmHg

Explanation:

position of Heart = 2m  below the top of the head  given that the neck is fully extended

Determine the minimum diastolic pressure

Minimum diastolic Pressure = 1060 kg/m^3 * 10 m/s^2 * 2m

                                               = 21200 kg/m^2

convert the value to Pa

21200 kg/m^2 = 21200 Pa

( 1 pa = 0.00750062 mmHg )

Therefore : minimum diastolic pressure = 21200 * 0.00750062 = 159.01 mmHg

The minimum diastolic pressure that a giraffe must have is 150 mmHg

Diastolic Blood pressure

Diastolic Blood pressure is the pressure on the walls of your arteries between heartbeats, that is when the heart is resting.

The minimum diastolic diastolic blood pressure = absolute minimum blood pressure a giraffe could have at the very top of its head.

Pressure formula

P = hpg

where;

P = pressure

h = height difference

p = density

g = acceleration due to gravity

Density of blood = 1000 kg/m^3

h =2.0 m

g = 10 m/s^2

Pressure = 1000 × 2.0 × 10

Pressure = 20000 Pascal or 20 kpa

Converting to mmHg

1 kpa = 7.50 mmHg

20 kpa = 20 × 7.50

Pressure = 150 mmHg

Therefore, the minimum diastolic pressure that a giraffe must have is 150 mmHg

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Question 2 of 26
What happens at a subduction zone between an oceanic plate and a
continental plate?
O A. The continental plate slides under the oceanic plate, and
part of it melts to form magma that sinks into the mantle.
O B. The oceanic plate and continental plate pull away from
each other, allowing magma to rise and form an ocean
ridge.
O c. The oceanic plate and continental plate slide past each
other, creating a series of faults that allow magma to rise
and form a chain of volcanoes.
O D. The oceanic plate slides under the continental plate, and
part of it melts to form magma that rises up through the
continental plate.
SUBMIT

Answers

D. The oceanic plate is more dense and will slide under the continental one

1. What features are located medial to the cranium and the mandible. Identify the category here.
2. How many individual items are included in this category?

Answers

The features that are located medial to the cranium and the mandible are:teeth,tongue, lips, chick hard andsoft palate,

   2. There is a total of six individual items in this category.

The human skull is made up of two parts, the cranium, which contains the face and the brain and the only movable bone called the mandible or lower jaw.

The features that are medial to the cranium and the mandible are those structures that are nearer to the mid-line.

The above mentioned features works together to provide the following functions of the cranium:

protection of the delicate structures including the brain, eye and the inner ear.maintaining patency of the nasal passage enabling breathing.the movement of the mandible allows chewing.

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.

In algae and plants, photosynthesis happens in the
vacuoles.
mitochondria.
chloroplasts.
chromosomes.

Answers

Answer:

chloroplast........................

Answer:

chloroplasts

Explanation:

they contain chlorophyll, a necessary element for photosynthesis

There is a Y chromosome gene in humans that has two alleles influencing hair growth on the pinna (external ear). One allele causes very hairy ears. The phenotypic effect of the other allele is to not have hairy ears. A man with hairy ears and a woman without hairy ears are starting a family. What is the probability their first child will be a girl with hairy ears

Answers

Answer:

Since the gene is located on the Y chromosome, and the Y chromosome is absent in females, the probability their first child will be a girl with hairy ears is zero

Explanation:

Y-linkage, also known as holandric inheritance is a form of sex linkage in which inherited traits in offsprings are produced by genes located on the Y chromosome. The Y chromosome is one of the sex-determining chromosomes and is present only in males. Males have a copy of the Y chromosome and an X chromosome while females have two copies of the X chromosome.

For a trait that is linked to the Ychromosome, the phenotypic effect occurs only in males and is always manifested in these males. Since the Y chromosome is absent in females, the character and its phenotypic effect are absent from daughters of trait carriers. Therefore, all daughters will be normal.

Since the gene for hair growth onnthe pinna is located on the Y chromosome, and the Y chromosome is absent in females, the probability their first child will be a girl with hairy ears is zero.

The probability that their first child will be a girl with hairy ears is zero.

The sex chromosomes in humans are the X and Y chromosomes. They determine the sex of a baby and also carry the sex linked traits. A baby girl results from XX  and a baby boy results from XY.

We are told that this gene for hairy ears is located on the Y chromosome. This chromosome is absent in a girl child. hence, the probability that their first child will be a girl with hairy ears is zero.

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I never thought of that these statements alone are not
sidered for a grade
Answer the questions) below
A former patient comes into the dental office and states that she has recently been diagnosed with skin cancer. She demands her xrays and treatment record and claims that she was exposed to unnecessary radiat
during her visits How should the dental assistant respond? Can the dental assistant be held responsible?

Answers

Answer:

geuueieiejrjejdjjhdjsjdjsjeiei do it is custom returns and more and more than more and then send it out in a no to a good morning to kya hua hai aapko ❤️❤️ ab workouts and more ab mai

The Big Bang is an idea that states that billions of years ago the universe began in a huge expansion of matter. This
idea has come about through numerous experiments performed by scientists including Edwin Hubble, Albert
Einstein, and Robert Wilson, all of whom revised and added to it over time The Big Bang has not yet been proven as
fact
The Big Bang can be best described as a(n)
experiment
hypothesis
theory
law

Answers

Answer:

The Big Bang theory is the prevailing cosmological model explaining the existence of the observable universe from the earliest known periods through its subsequent large-scale evolution. The model describes how the universe expanded from an initial state of high density and temperature,and offers a comprehensive explanation for a broad range of observed phenomena, including the abundance of light elements, the cosmic microwave background (CMB) radiation, and large-scale structure.

Crucially, the theory is compatible with Hubble–Lemaître law—the observation that the farther away a galaxy is, the faster it is moving away from Earth. Extrapolating this cosmic expansion backwards in time using the known laws of physics, the theory describes an increasingly concentrated cosmos preceded by a singularity in which space and time lose meaning (typically named "the Big Bang singularity"). Detailed measurements of the expansion rate of the universe place the Big Bang singularity at around 13.8 billion years ago, which is thus considered the age of the universe.

After its initial expansion, an event that is by itself often called "the Big Bang", the universe cooled sufficiently to allow the formation of subatomic particles, and later atoms. Giant clouds of these primordial elements—mostly hydrogen, with some helium and lithium—later coalesced through gravity, forming early stars and galaxies, the descendants of which are visible today. Besides these primordial building materials, astronomers observe the gravitational effects of an unknown dark matter surrounding galaxies. Most of the gravitational potential in the universe seems to be in this form, and the Big Bang theory and various observations indicate that this excess gravitational potential is not created by baryonic matter, such as normal atoms. Measurements of the redshifts of supernovae indicate that the expansion of the universe is accelerating, an observation attributed to dark energy's existence.

Georges Lemaître first noted in 1927 that an expanding universe could be traced back in time to an originating single point, which he called the "primeval atom". Edwin Hubble confirmed through analysis of galactic redshifts in 1929 that galaxies are indeed drifting apart; this is important observational evidence for an expanding universe. For several decades, the scientific community was divided between supporters of the Big Bang and the rival steady-state model which both offered explanations for the observed expansion, but the steady-state model stipulated an eternal universe in contrast to the Big Bang's finite age. In 1964, the CMB was discovered, which convinced many cosmologists that the steady-state theory was falsified, since, unlike the steady-state theory, the hot Big Bang predicted a uniform background radiation throughout the universe caused by the high temperatures and densities in the distant past. A wide range of empirical evidence strongly favors the Big Bang, which is now essentially universally accepted.

Explanation:

Answer:

Explanation:

its a theory

why do proteins not pass through plasma membranes

Answers

Answer:

because protein molecules are very large in molar masses

differences between a fish and a toad.​

Answers

Answer:

⬇ See answer below ⬇

Explanation:

A fish is a(n) aquatic animal which has gills and can only find oxygen underwater. Toads are quite different though as they are amphibians who can live on both land and underwater. (Hope this helped you!)

Defined forestry and its important in nepal?​

Answers

Explanation:

In a mountain- ous country such as Nepal, forests are important for the protection of water catchments, the conservation of soil and the maintenance of biodiversity, as well as for their contributions to sustainable rural livelihoods and the maintenance of the environment.

Answer:

forest is defined as a large area that has many trees and other plants, or a dense area that is like a forest.

its important in nepal are:

1.for the protection of water catchments,

2.for the conservation of soil and

3.for the maintenance of biodiversity,

4.. for their contributions to sustainable rural livelihoods

5.the maintenance of the environment.

6.it increase the natural beauty of nepal

हरियो वन नेपालको धन

जय नेपाल

What are physical and chemical properties

Answers

physical property: Any characteristic that can be determined without changing the substance's chemical identity. chemical property: Any characteristic that can be determined only by changing a substance's molecular structure.

3. Explain the main difference between organisms of the
domains Bacteria and Archaea and organisms of the domain
Eukarya.

Answers

Answer:

Both Bacteria and Archaea are prokaryotes, single-celled microorganisms with no nuclei, and Eukarya includes us and all other animals, plants, fungi, and single-celled protists – all organisms whose cells have nuclei to enclose their DNA apart from the rest of the cell.

Explanation:

Both Bacteria and Archaea are prokaryotes, single-celled microorganisms with no nuclei, and Eukarya includes us and all other animals, plants, fungi, and single-celled protists – all organisms whose cells have nuclei to enclose their DNA apart from the rest of the cell.

Hemophilia is an X-linked recessive condition in which blood does not clot properly. Queen Victoria of England had one allele for hemophilia. Most of her male descendants had the disorder, but few females had it. Why did hemophilia occur more frequently in Queen Victoria’s male descendants?

Answers

Answer:

Because hemophilia is an X-linked recessive condition.

Explanation:

As the problem states, hemophilia is an X-linked recessive condition.

Meaning that in Queen Victoria's female descendants (coded XX) which inherited the Queen's one allele, for hemophilia to appear in them it would be necessary to inherit another hemophillia-carrying allele from the father.The Queen's male descendants (coded XY) would always manifest hemophilia if they inherited the Queen's hemophilia allele, as they possesed only one X gene.

While snorkeling in the ocean you find large, leafy organisms that are brownish in color growing in large masses. You are unsure whether they are protists since they look quite plant-like. If they are actually protists, what characteristic will the organism's cells exhibit?

A. Each of the cells in the organism will have its own nucleus.

B. The cells will form a colony with separate groups of cells doing various jobs.

C. The cells will form a colony without differentiating into separate tissues.

D. Most of the cells in the organism will have cilia or flagella.

Answers

Answer:

A. Each of the cells in the organism will have its own nucleus.

Explanation:

While snorkeling in the ocean you find large, leafy organisms that are brownish in color growing in large masses. To be sure that they are protists since they look quite plant-like, the characteristic that the organism's cells exhibit will be that each of the cells in the organism will have its own nucleus.

This is because protists are eukaryotes, which simply means it has its own nucleus and are usually single celled.

IN____________ NUTRITION , THE ORGANISMS DEPEND UPON ITS HOST FOR ITS FOOD .

Answers

Answer:

heterotrophic nutrition!!

define cell and atom.....​

Answers

Answer:

cells are the basic building blocks of all organisms , whether living or dead.

atoms are the basic building blocks of matter(non-living things) . every item , for eg, chair, book, toothbrush are made of atoms?

In the lung alveoli, the oxygen partial pressure is about 100 mm of mercury, or 0.13 Atm. If blood was solely composed of a saline solution (lacking any hemoglobin), oxygen equilibrates with the alveolar air to a concentration of about 3 mg/L or 0.093 mm). In oxygen-saturated blood, however, the O2 concentration is about 0.008 M (8 mm).

Required:
a. What is the Henry's Law constant for oxygen in saline?
b. What is the Henry's Law constant for oxygen in blood?
c. How much does the presence of hemoglobin enhance oxygen solubility in blood (i.e. what is the ratio in blood vs saline)?

Answers

Answer:

Picture is attached.

why are electrons in an atom involved in bonding

Answers

Answer:

The valence electrons have a role in the bonding of two atoms. The nuclei of each atom are drawn together by their attraction to the valence electrons of the other atom. As the atoms are drawn together by their attractions, electrons from each atom are drawn to the nuclei of both atoms, where they are "shared."

OAmalOHopeO

Find the number of chromosomes are in each daughter cell when a sperm cell with a diploid number of 26 chromosomes undergoes meiosis process​

Answers

Answer:

At the end of mitosis, the two daughter cells will be exact copies of the original cell. Each daughter cell will have 30 chromosomes.

Erosion and deposition constantly change Earth’s surface. Erosion carries natural materials like rock and soil from one place to another. Through deposition, these natural materials may be deposited in areas where they build up over time.

Which landform results from the deposition of materials in a valley during volcanic eruptions?

A.sill
B.mud pot
C.caldera
D.lava plateau
(Science)

Answers

D. Lava plateau
They are formed when lava flows from the volcano & build up over time as the molten lava is deposited & cools.

Which of the following was not part of the original cell theory, but was added later after we learned more about cellular structure​

Answers

where is full question man

Quick quick !!

Which problem affects the global economy in addition to a local economy?
A. A citywide power outage
B. Drought on an island
C. Water pollution in a lake
D. Pollution of the atmosphere

Answers

Answer:

D. Pollution of the atmosphere

Explanation:

All the other answers would seem to only affect the designated local area. However, option D would affect both the local and global economy.

The statement that there must be a preexisting cell in order for a new cell to form is known as cell

Answers

Answer:

cells duplicate so when there is no cell it cant make any

Explanation:

The statement that there must be a preexisting cell in order for a new cell to form is known as cell theory.

What is cell theory?

The scientific idea that cells make up living organisms, that they are the fundamental structural and organizational unit of all species.

They all originate from previously existing cells is known as cell theory. It was first proposed in the middle of the nineteenth century.

Because cells are the building blocks of all life, cell theory is essential for our understanding of biology.

We can have single-celled organisms like yeasts and bacteria. All living things grow and develop on the basis of cell division, or the division of a cell from one to two to four.

A vital process for life is mitosis. A cell divides into two identical daughter cells after duplicating all of its components, including its chromosomes.

Thus, the statement "cell arises from pre-existing cells" comes from cell theory.

For more details regarding cell theory, visit:

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In human populations of northern European origin, the disorder called hemochromatosis causes excess iron uptake from food and affects one in 200 adults. Among individuals 15 to 50 years old, men are ten times as likely as women to suffer from iron overload. Part A Taking into account this range of ages, what explains this difference

Answers

Answer:

Females of reproductive age lose iron through menstrual blood

Explanation:

Iron is an essential micronutrient involved in the formation of hemoglobin, myoglobin and many enzymatic groups. Moreover, hemochromatosis is a genetically inherited disorder caused by mutations in genes associated with the processes of absorption, transport and storage of iron. The excess of iron is stored in different organs (e.g., liver, heart, pancreas, etc), causing characteristic tissue damage. During each menstrual cycle, women and girls suffer a blood loss of approximately 40 ml, which is associated with an average loss of 1.6 mg of iron. In consequence, it is expected that women of reproductive age (ie, 15–49 years) lose iron during their menstrual periods, thereby avoiding iron overload caused by hemochromatosis.

Name ten infrasonic animals.

Answers

Whales, rhinos, squid, cuttlefish, hippos, elephants, octopus, pigeons, squid, cod, guinea fowl

animals with scales on skin are called?



Answers

Answer:

Animals with scales on their skin are called reptiles..

hope it helps

stay safe healthy and happy...

make slogan about showing care of environment and natures​

Answers

plant trees at least every year and keep atmosphere clear

Which is a source of adult stem cells?
bone marrow
amniotic fluid
embryos
gametes

Answers

Answer:

Bone marrow is a source of adult stem cell

Which genotype would give you a wild type phenotype?

Answers

Answer:

C

Explanation:

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