The answer to whether primers are reusable for each cycle is no. Primers are used only once during each cycle of PCR and must be replaced for each subsequent cycle.
In molecular biology, primers play a crucial role in the polymerase chain reaction (PCR) process. PCR is a technique used to amplify DNA samples, and primers act as the starting points for the replication process.
There are several reasons for this.First, the primers become depleted after each cycle of PCR. This is because the primers bind to the target DNA, initiating the replication process. However, as the process continues, the primers are used up and must be replaced.Second, the PCR process can generate secondary structures in the primers that interfere with the binding of new primers in subsequent cycles. This means that even if there are enough primers present, they may not be able to bind effectively to the target DNA.Third, the PCR process can result in the formation of unwanted products that can interfere with subsequent cycles. These products can also bind to primers, making them less effective.To learn more about primers here:
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explain why it would not be possible to accurately measure hemoglobin concentration if the rbcs were not first lysed quizlet
To accurately measure hemoglobin concentration, it is important to first lysed the RBCs to release the hemoglobin into solution and ensure that it is in a form that can be easily measured. This will help to eliminate interference from the cell membrane and prevent hemoglobin aggregation, allowing for more accurate results.
Hemoglobin is a protein that is responsible for carrying oxygen in the red blood cells (RBCs). Accurate measurement of hemoglobin concentration is important in diagnosing various health conditions. However, it is not possible to accurately measure hemoglobin concentration if the RBCs are not first lysed, or broken down.
This is because the hemoglobin is located inside the RBCs and is not readily accessible for measurement without first breaking down the RBCs.
Lysis of the RBCs allows for the release of hemoglobin from the RBCs into the surrounding fluid, making it accessible for measurement. When RBCs are intact, the hemoglobin is surrounded by a cell membrane, which makes it difficult to accurately measure the concentration of hemoglobin in the sample. The cell membrane can interfere with the measurement, leading to incorrect results.
Additionally, when RBCs are not lysed, the hemoglobin may aggregate or clump together, making it even more difficult to measure accurately. Clumped hemoglobin will not disperse in solution, making it difficult for the measurement techniques to properly detect and quantify it.
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Name the family of proteins that by physically interacting with ras enhances the intrinsic gtpase activity of ras in the rtk-mediated signal transduction pathway.
The family of proteins that enhance the intrinsic GTPase activity of Ras in the RTK-mediated signal transduction pathway is called GAP (GTPase Activating Proteins).
GAPs bind to Ras and increase its rate of hydrolysis of GTP to GDP, leading to the termination of Ras signaling. This helps to prevent overactive or prolonged signaling that can lead to cellular malfunctions and diseases such as cancer. Thus, GAPs play a crucial role in the regulation of the Ras signaling pathway, ensuring that the proper signals are transmitted and the pathway is terminated at the right time.
The term "family of proteins" is used because there are multiple different types of GAP proteins, each with slightly different structures and functions. The GAP family is made up of several different proteins, including p120GAP, NF1-GAP, and p190GAP, among others.
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T/F : neutralizes the acidic chyme as it enters the small intestine.
True , The bicarbonate from the pancreas helps to neutralize the acidic chime and the bile from the gall bladder aids in the digestion of fat.
In inorganic chemistry, bicarbonate is a transitional phase in the deprotonation of carbonic acid . It has the chemical formula HCO and is a polyatomic anion. A vital metabolic function in the physiological pH buffering system is played by bicarbonate. The English scientist William Hyde Wollaston first used the word "bicarbonate" in 1814. The name endures as a meaningless name. The bicarbonate ion, also known as the hydrogen carbonate ion, is an anion with the empirical formula and a molecular mass of 61.01 Daltons. Its structure is made up of a central carbon atom that is surrounded by three oxygen atoms in a trigonal planar arrangement, with a hydrogen atom attached to one of the oxygens.
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which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments?
Electrophoresis is a method for separating and identifying biomolecules based on their size, charge, and structure, such as DNA, RNA, or proteins. To observe the separated fragments.
the gel is frequently dyed with a dye, and the optimum gel for depicting the separation and identification of the fragments will depend on the specific needs of the experiment, the electrophoresis settings utilized, and the interpretation of the data. Electrophoresis is a laboratory method for separating and analyzing charged biomolecules like DNA, RNA, or proteins. The separation is based on the movement of molecules across a gel matrix in an electric field. The gel matrix acts as a physical barrier, slowing the movement of bigger molecules and allowing them to be separated.on size. A voltage is applied across the gel to produce the electric field, and the biomolecules are put onto one end of the gel.
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What level of protein structure is associated with the sequence of amino acids?A. PrimaryB. SecondaryC. TertiaryD. Quarternary
The level of protein structure associated with the sequence of amino acids is (A) Primary.
Peptides and proteins both have a fundamental structure that can be described as the linear sequence of their amino acids. The term "primary structure" refers to the organized and sequential arrangement of amino acids that are contained within a protein. Proteins are constructed from a linear chain of just twenty different amino acids, each of which possesses a unique side chain.
The side chains of amino acids each have their own unique chemical composition. The fundamental structure of a protein is only the arrangement of the amino acids in a polypeptide chain.
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the majority of the energy captured in the krebs cycle is in the form of nadh.
NADH, or nicotinamide adenine dinucleotide with its reduced form, is an important coenzyme found in the mitochondria of cells. NADH is produced in the citric acid cycle, also known as the Krebs cycle.
This coenzyme (NADH) carries electrons from the breakdown of glucose molecules from the citric acid cycle to the electron transport chain, where it is then oxidized to NAD+ and releases energy that is used to synthesize ATP molecules. The Krebs cycle is an of eight steps that occur within the inner mitochondrial matrix. In the citric acid cycle, NADH is produced as the result of oxidation-reduction reactions. During the Krebs cycle, two molecules of NADH are produced for every molecule of glucose that is broken down. This energy is used to create ATP molecules, which are then used to power metabolic processes.
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jack summers has hit his head and is diagnosed with damage to his hypothalamus. physicians are postulating what will happen if jack's hypothalamus is not secreting any hormones. what hormones will not be produced?
Many hormones are normally produced by the hypothalamus, including GnRH, dopamine somtostatin, prolactin releasing hormone, TRH HORMONE, corticotropin releasing hormone, and many others. So, if Jack's hypothalamus area is injured, he will not emit the above-mentioned hormone, which is required for bodily function.
GnRH, dopamine somtostatin, prolactin releasing hormone, TRH HORMONE, corticotropin releasing hormone, and many more hormones are routinely generated by the hypothalamus. As a result, if Jack's hypothalamus is damaged, he will not produce the aforementioned hormone, which is essential for physical function.
The hypothalamus is a brain organ that regulates your hormone system. It transmits hormones to another portion of your brain called the pituitary gland, which then delivers hormones to your other organs.
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Main part of a nerve cell; contains organelles to maintain homeostasis inside each cell
Mithocondria. The power plant of the cell, this organelle is crucial for supplying energy for vital metabolic functions that enable the cell to achieve homeostasis.
Cellular membrane It is a biphospholipidic layer made up of transversal proteins that link the external environment to the inside of the cell. The cell membrane is essential for communication and for providing hints about the environment to the cell, enabling adjustments aimed at maintaining homeostasis. Nucleus. The code that includes all the information the cell needs to live is found in this organelle. The nucleus receives and transmits signals that cause metabolic adjustments, which is essential for flexibility and enabling homeostatic conditions in a changing environment.
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if a dna sample were composed of 20% guanine, what would be the percentage of cytosine?
a. 40
b. 30
c. 60
d. 20
According to Chargaff's rule, If a DNA sample were composed of 20% guanine, D. 20 would be the percentage of cytosine.
Any species or organism's DNA should contain the same amount of guanine and adenine as its cytosine and thymine, according to Chargaff's rule. Additionally, the stoichiometric ratio of purine and pyrimidine bases ought to be 1:1.
Guanine is a purine, whereas cytosine is a pyrimidine. Chargaff's rule states that the concentration of guanine should be the same as that of cytosine because guanine and cytosine are always paired in DNA. Therefore, if the guanine content is 20%, the percentage of cytosine should also be 20%.
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the average molecular weight of an amino acid in proteins is 113, taking into account their average relative abundances. calculate the number of amino acid residues in a protein whose molecular weight equals 52,728.
The number of amino acid residues in a protein whose molecular weight equals 52,728 is 467 residues.
To calculate the number of amino acid residues in a protein of molecular weight 52,728, we can use the following formula:
Number of residues = Molecular weight of protein / Average molecular weight of an amino acid
The average molecular weight of an amino acid is 113, so the calculation would be:
Number of residues = 52,728 / 113 = 467.3 (rounded to 467 residues)
Therefore, a protein with a molecular weight of 52,728 would contain approximately 467 amino acid residues. Please note that this is just a rough estimate and the actual number of residues can vary depending on the specific amino acids and their arrangement in the protein.
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what is the outcome of evaporative cooling in the form of sweating? what is the outcome of evaporative cooling in the form of sweating? a decrease in body temperature a decrease in air temperature a decrease in blood ph
The outcome of evaporative cooling in the form of sweating is A decrease in body temperature.
Evaporative cooling is a process by which heat is removed from the body through the evaporation of sweat. When sweat evaporates from the surface of the skin, it takes heat away with it, causing a cooling effect. This process helps regulate body temperature and maintain homeostasis.
Sweating is the body's natural way of dissipating excess heat, and it is most effective in warm, humid environments where the rate of evaporation is high. When the body needs to cool down, the sweat glands are activated, producing sweat that then evaporates, removing heat from the body and causing a decrease in body temperature. This decrease in temperature helps keep the body within its optimal temperature range, maintaining overall health and wellness.
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a student observes a cell through a light microscope and correctly decides that it is a plant cell. what observation would lead to that conclusion?
When a student examines a cell under a light microscope, they correctly identify it as a plant cell. That conclusion would follow from the observation that it has a sizable central vacuole.
Large rectangular interlocking blocks can be seen as plant cells under a microscope. Each cell's exterior cell wall is clearly discernible. The cell wall is reasonably thick and may be clearly seen when dyed. Additionally, the cytoplasm is faintly stained and has a nucleus at the cell's edge that is darkly stained.
In addition to a cell membrane, plant cells also have a cell wall. The cell membrane is encased by the cell wall in plants. The plant cell's distinctive rectangular shape is a result of this. Animal cells do not have a cell wall; only the cell membrane is present.
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A bacterium that only possesses the ability to ferment obtains energy A by glycolysis only. B. by fermentation or aerobic respiration. C. only in the presence of oxygen. D.by aerobic respiration only. E. only in the absence of oxygen.
A bacterium that only possesses the ability to fermentation obtains energy by glycolysis only.
Glycolysis is the first stage of fermentation; however, it does not involve the final two phases of aerobic cellular respiration (the Krebs cycle and oxidative phosphorylation). Two NAD+ electron carriers are converted during glycolysis into two NADH molecules and two net ATPs. For glycolysis to proceed and for cells to continue generating 2 ATPs, the NADH must be oxidized again. Because oxidative phosphorylation cannot occur during fermentation due to a lack of oxygen, cells are only able to produce 2 ATP at a time. Alcoholic and lactic acid fermentation are the two forms of fermentation. We use both forms of fermentation using other organisms even though our cells can only execute lactic acid fermentation.
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how breast milk is formed in mammary glands
Breast milk is produced by the mammary glands which are made up of lobules, which are clusters of glandular cells that produce milk. Breast milk is produced when a hormone called prolactin stimulates the mammary glands to produce milk. Prolactin is released by the pituitary gland in response to the hormone stimulation from the hypothalamus, which is triggered by the baby's suckling on the nipple.
The mammary glands are a type of exocrine gland found in female mammals, including humans, and are responsible for producing and secreting breast milk. The milk is stored in ducts, which lead to the nipple.
Breast milk is a complex fluid that contains various nutrients, hormones, and antibodies. These components provide vital nutrition to the baby and help to protect them against infections and illnesses. Some of the key nutrients in breast milk include proteins, carbohydrates, fats, vitamins, and minerals.
Breast milk also contains antibodies that help to build the baby's immune system. These antibodies are transferred from the mother to the baby, providing protection against various infections and illnesses. Additionally, hormones in breast milk, such as oxytocin and prolactin, promote bonding and a sense of comfort between the mother and baby.
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White-crowned sparrows evolved from an ancestral species that possessed the capacity for song learning. This is an example of...
A. an adaptive value explanation.
B. a developmental explanation.
C. an explanation relating to evolutionary history.
D. a physiological explanation
White crowned sparrows evolved from their ancestral species which possessed the capacity for song learning. This is an example of an explanation related to evolutionary history.
Hence, the correct option is option C.
Evolution is basically the change in the heritable characteristics of the biological populations over a number of successive generations. These characteristics can be the expressions of the genes that are passed on from the parent to the offspring when reproduction takes place.
The white crowned sparrows basically evolved from their ancestors which had a capacity to learn songs and this is an example of explanation which is related to the evolutionary history.
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NEED HELP ASAP
Where do you think the waves in the ocean come from and what makes the waves. Research how waves in the ocean are formed. Write a two-three paragraph
According to the research, the correct answer is Option D. Waves are ripples that form on the surface of the sea due to the friction generated by the wind on the water.
How waves in the ocean are formed?It is created on the water surface, usually in the ocean as a result of the wind, which exerts a force on the liquid.
In this sense, the water depth, the area subjected to the wind and its speed are some of the factors that affect the greater or lesser size of waves in the ocean.
Therefore, we can conclude that according to the research, the waves in the ocean are formed by the wind, upon contact with sea water.
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Wrinkled eed are receive to mooth eed. Show a plant that alway produce wrinkled eed croed with a heterozygou mooth eed producing plant
The genotype ratio is 1:2:1, often known as RR: Rr: Rr. The ratio of round to wrinkled seeds in phenotypic is 3:1.
When compared to females, males have XY chromosomes. A heritable mutation is a change in the DNA's nucleotide sequence. Among the 23 pairs of chromosomes found in humans, there are 22 pairs of autosomes and 1 pair of sex chromosomes. The gamete will therefore have 23 chromosomes. The term "genotype" is used in a broad sense to refer to either the genes themselves or the whole gene pool of an organism. The phrase can also refer to a gene's alleles, or distinctive variations, in a more limited sense. A scalar variable called speed can be used to describe how quickly an item moves or how much its location changes over time. It is often shortened to "s."
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it is a transport protein present in the blood. Fat
Glucose
Hemoglobin
Answer: Hemoglobin
What is Hemoglobin?
In normal adults, hemoglobin is a protein whose primary function is to carry oxygen from the lungs to the tissues and carbon dioxide from the tissues to the lungs.
Hemoglobin is known as Transport Protein due as it:
Carries oxygen from the lungs to the tissues.Carries carbon dioxide from the tissues to the lungs.It gives red blood cells their colour and shape.Hemoglobin is essential for the body because it can bind oxygen, carbon dioxide, and other groups such as sulfides and nitric oxide. These substances are transported through blood bound to hemoglobin.
Therefore, known as transport protein.
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Hemoglobin is a transport protein present in the blood.
What is Hemoglobin?
In normal adults, hemoglobin is a protein whose primary function is to carry oxygen from the lungs to the tissues and carbon dioxide from the tissues to the lungs.
Hemoglobin is known as Transport Protein due as it:
Carries oxygen from the lungs to the tissues.
Carries carbon dioxide from the tissues to the lungs.
It gives red blood cells their colour and shape.
Hemoglobin is essential for the body because it can bind oxygen, carbon dioxide, and other groups such as sulfides and nitric oxide. These substances are transported through blood bound to hemoglobin.
Therefore, known as transport protein.
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Ariana walked into a damp, sealed-off cellar where she discovered a body that was covered in a waxy substance. What process MOST likely took place
when this body decomposed?
O A. adipocere
B.
mummification
O C. skeletonization
OD.
putrefaction
tree annual rings 1 10 2 15 3 7 how would the position of the vascular cambium in these three core samples compare?
Begin counting the first dark ring you notice in the centre of the stump or cross-section of wood. Count outwards from the center ring until you reach the final dark ring.
The total number of dark rings indicates the tree's age in years. (The bark of the tree does not count as a dark ring.
In all three situations, the vascular cambium would be in different places relative to the stem Center, increasing in distance from 3 to 1 to 2. The tree will die if the cambium layer is damaged, although not immediately. According to most experts, if the cambium layer is weakened, it can take six to ten years for a tree to degrade and die.
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How many grams of protein did you consume on this day? The only foods recordedon this day should be the eggs, bacon, and whole wheat bread sample breakfast.ChoicesA. 15.30 gB• 22.87 gC. 26.46 gD. 34.90 g
34.90 grams of protein did you consume on this day. When the foods recordedon this day should be the eggs, bacon, and whole wheat bread sample breakfast. so, the right choice is D.
Six grammes of protein are present in one quarter cup of egg replacements.
Compared to grilled Canadian bacon, which offers 2 grammes of protein per slice, cooked hog bacon has 3 grammes of protein per slice. Turkey bacon cooked to order includes 4.74 grammes of protein per 1-ounce serving.
Regular wheat or white bread has 2 grammes of protein per 1-ounce slice, whereas lighter or thinner crust versions with fewer calories have 1 gramme. A ordinary English muffin and a kaiser roll with a diameter of 3 1/2 inches both include 3 grammes of protein.
A sandwich with bacon and eggs can include a minimum of 13.3 grammes of protein.
Two extra large eggs and three strips of bacon make up a more common sandwich, which has 27 grammes of protein. The sandwich will have an additional 7 grammes of protein per ounce if cheese is added. Adults are advised to consume 0.8 grammes of protein per kilogramme of body weight per day. You can fulfil your protein needs by consuming an average-sized bacon and egg sandwich, but only in moderation. The high levels of fat, sodium, and cholesterol in this sandwich can cause weight gain and raise your risk of developing heart disease. Egg whites with 1 ounce of Canadian bacon on whole-wheat toast might be a healthier .
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Consider a locus in which the fitness of aa and aa genotypes are greater than the fitness of aa genotype. In addition, this locus experiences forward mutation from the a allele to a allele. At this locus, the a allele will:.
In addition, this locus experiences forward mutation from the a allele to a allele, at this locus, the a allele Will never go to fixation.
The abrupt, heritable alteration in an organism's genetic sequence is known as a mutation. The organisms' unique sickness could be brought on by the mutation.
A mutation in biology is an adjustment to the nucleic acid sequence of an organism's, virus's, or extrachromosomal DNA. DNA or RNA can be found in the viral genome.
Increased gene frequency in following generations is caused by improved gene fitness. When selection and mutation are absent, the gene will stabilize in the population. Due to the fact that mutation is occurring, as indicated by the facts provided in the question, the A allele will never become fixed in the population.
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Consider a locus in which the fitness of AA and Aa genotypes are greater than the fitness of aa genotype. In addition, this locus experiences forward mutation from the A allele to a allele. At this locus, the A allele:
a. will always go to fixation.
b. will always be lost.
c. will never go to fixation.
d. will never change.
e. None of the above.
dolphins make sounds in air and water. what is the ratio of the wavelength of the sound in air to its wavelength in water? assume air temperature is 20.0°c and the speed of sound in sea water at 20.0°c is 1540 m/s.
Explanation:
The ratio of the wavelength of sound in air to its wavelength in water can be calculated using the equation for the speed of sound in air and water. The speed of sound in air is dependent on temperature and pressure, and the speed of sound in water is dependent on temperature and the properties of the medium (e.g. salinity, pressure, etc.).
At 20.0°C, the speed of sound in air is approximately 340 m/s. The wavelength in air is given by the formula:
λ_air = v_air / f
where λ_air is the wavelength in air, v_air is the speed of sound in air, and f is the frequency of the sound.
The wavelength in water is given by the formula:
λ_water = v_water / f
where λ_water is the wavelength in water, v_water is the speed of sound in water (1540 m/s at 20.0°C), and f is the frequency of the sound.
The ratio of the wavelength of sound in air to its wavelength in water is given by:
λ_air / λ_water = v_air / v_water
Plugging in the known values for v_air and v_water, we find that:
λ_air / λ_water = 340 m/s / 1540 m/s = 0.22
So, the wavelength of sound in air is approximately 0.22 times the wavelength of the same sound in water.
the endocrine system appears to play a role in which types of disorders?
Menopause.
Diabetes.
Addison's disease.
Cushing's disease.
Graves' disease.
Hashimoto thyroiditis.
Hyperthyroidism/hypothyroidism.
Prolactinoma.
What Does Niche Mean in Ecological Biology?
The term "niche" in ecology refers to the function an organism performs within a community.
What is a biological niche, using an example?The function a species performs is referred to as its ecological niche.A niche goes beyond the diet and habitat of an organism.An oak tree develops to dominate the forest canopy and converts sunshine into food, while a garden spider hunts its prey among plants.
What is Biology 12's ecological niche?The ecological niche explains the relationships between species in an environment.The ability of the a species to thrive and persist depends on both biotic (living) and abiotic (non-living) variables, which together define a species' niche.
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Three hundred joules of heat are added to a system. The system uses 140 joules to do work. By how much did the internal energy of the system change?.
A system generates 300 J of heat and uses 140 J to accomplish a particular task. Since the system's internal energy will change by 160 J, option B is correct.
The energy transfer that occurs when an object is moved over a length by an external force, at least some of which is applied in the direction of the displacement, is referred to as work in physics. If the force is constant, work can be calculated by multiplying the elements of the force acting along the path by its length.
This idea can be expressed mathematically as, where W is the amount of work and f is the force multiplied by d, the distance. When the force is applied at an angle of about the displacement, the work is finished.
The information provided in response to the question is,
Energy required = 140 J
Energy added = 300 J
Change in internal energy in the system after doing work.
= 300 J- 140 J
= 160 J
Therefore, the change in the internal energy of the system is 160 J.
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A fish inherits a mutation that results in a new regulatory switch ("eye switch") that regulates Pitx1 expression in the eye. This new switch binds a particular activator found in the tissues of the eye (activator 3)
The fish with the eye switch mutation will likely experience changes in Pitx1 expression specifically in the tissues of the eye, as the new switch regulates its expression in response to the presence of activator 3.
The fish "eye switch" mutation points to the creation of a novel regulatory system that controls Pitx1 gene expression. The patterning, differentiation, and other stages of ocular development are all influenced by this gene. An activator known as activator 3 that is frequently found in ocular tissues is bound by the novel switch. When activator 3 is present, the eye switch will be activated, which will change how the Pitx1 gene expresses.
This alteration in the expression of Pitx1 in response to activator 3 may have substantial effects on the development and function of the eye.
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The complete question is:
A fish inherits a mutation that results in a new regulatory switch ("eye switch") that regulates Pitx1 expression in the eye. This new switch binds a particular activator found in the tissues of the eye (activator 3), where will the Pitx1 expression changes are going to occur?
T/F : calcitonin is the main regulator of blood calcium levels.
Answer: False
Explanation:
Which of the following is not a type of
fatty acid?
A. Presaturated fat
B. Saturated fat
C. Unsaturated fat
D. None of the above
Answer:
The answer is D, because it is none of the above.
Explanation:
All of the above answers are fatty acids.
Forensic scientists are considering doing DNA tests on many pieces of evidence they currently have in their lab. What item would be LEAST likely to
produce suitable DNA test results?
A. a bloodied shirt stored in a paper bag
B.
buccal cells collected two days ago
a finger that was stored in formaldehyde
D. hair samples that were taken from a small animal
C.
DNA proof, these difficulties include the sufficiency of demographic studies and testing procedures, the significance of human error in interpreting test findings, alleged injustice to criminal defendants, and the absence of standards.
Which DNA types can be used in forensic investigations?Utilizing DNA profiling is one example of contemporary forensic evidence. DNA can be found in several materials, such as bone, tissue, semen, hair, and saliva. Forensic uses of fingerprints include their detection.When biological evidence is available, DNA can be used to identify criminals with astounding accuracy. By the same token, DNA can be used to clear suspicions and acquit those who have been falsely accused or found guilty of crimes.These difficulties include the sufficiency of demographic studies and testing procedures, the significance of human error in interpreting test findings, alleged injustice to criminal defendants, and the absence of standards.The complete question is,
What difficulties arise when obtaining DNA proof?
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