In actuality, the DNA sequence of the human genome is NOT random. Some sequences, including some very large sequences, are repeated many times throughout the human genome. Write two ideas you have for how this fact complicates the use of CRISPR gene-editing technology in humans.

Answers

Answer 1

Answer:

repeated DNA sequences decrease the target specificity of the CRISPR-Cas system, and may lead to off-target effects  

Explanation:

The CRISPR-Cas system is a versatile and powerful tool for gene editing. This gene-editing tool consists of a single guide RNA (sgRNA) that guides the Cas endonuclease protein to the appropriate genomic locus, where Cas generate a double-strand break in the DNA and thus induces DNA repair either by Non-homologous end joining (NHEJ) or Homologous Recombination (HR) repair pathways. The sgRNA consists of a 17-20 nucleotide sequence which is complementary to the target DNA, this sequence is called crispr RNA (crRNA). The existence of repeated DNA sequences hampers the specificity of the CRISPR-Cas tool because a particular sgRNA is able to guide Cas9 to different genomic loci that have the same (repeated) nucleotide sequence complementary to the crRNA. In consequence, repeated DNA sequences may lead to undesired off-target effects, i.e., unintended cleavage (and therefore unintended mutations) at untargeted genomic sites.


Related Questions

What is a real world example of a molecular pump in the human body?

Answers

Answer:

The most common example of a molecular pump in the human body is the pump for sodium and potassium.

Explanation:

Sodium ions and ATP bind to the pump to use energy to change the shape. The sodium then leaves and the potassium can now bind due to the shape change. The potassium ions are then released and the pump goes back to normal for the cycle to repeat again.

There are many cells in your body that use pumps to move molecules. For example, your nerve cells (neurons) would not send messages to your brain unless you had protein pumps moving molecules by active transport. The sodium-potassium pump (Figure below) is an example of an active transport pump! Have a nice day

You are given a sequence of DNA and told that it is human. You are asked to find out its identity and whether it has similarity to sequences in other organisms. Please describe the bioinformatics tool, the database, and the procedure you would use to find such information. Give two possible outcomes of your search.

Answers

Answer:

The summary of the question has been summarized throughout the explanation segment below.

Explanation:

BLAST seems to be the most commonly utilized sequencing correlation platform. BLAST seems to be a methodology as well as an operation for the comparison of primary physiological sequence data. Instead with BLAST, we're going enough to get some successfully predicted gene mutation new variant. Look for, racial identity percentage points and sometimes even E Value percentage points lookup pattern.

This should start giving extensive information such as which life forms the component has been transcribed. And we'll get documentation about everything from the gene, exon, and so on., connection to the enzyme interpretation.

What are four conditions in the body related to maintaining homeostasis?

Answers

Answer:

Regulation of body temperature, blood pressure, PH and glucose concentration are four examples of how the body maintains homeostasis. hope this helps

Why must transcription occur where DNA can be found?

because DNA can't leave

O because DNA polymerase is found there

O because ribosomes are in the nucleus

O because helicase unzips the DNA

Answers

Answer:

because DNA can't leave

Explanation:

Transcription must occur where deoxyribonucleic acid (DNA) can be found: A. because DNA can't leave.

What is transcription?

Transcription can be defined as a biological process that typically involves re-writing the information contained within a deoxyribonucleic acid (DNA) into a ribonucleic acid (RNA) by enzyme RNA polymerase.

Generally, the Transcription of deoxyribonucleic acid (DNA) produces a single-stranded ribonucleic acid (RNA) molecule that is the same with the strand of DNA used as the template.

In conclusion, transcription must occur where deoxyribonucleic acid (DNA) can be found in living organisms because DNA can't leave.

Read more on transcription here: https://brainly.com/question/19649964

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How is the nitrogen cycle important to humans?
OS
It produces free nitrogen that humans can breathe.
It converts nitrogen into a form that humans can obtain by eating other organisms.
It produces nitrogen compounds that humans can breathe.
It converts nitrogen into a form that humans can obtain by absorbing it through their skin.

Answers

Humans obtain nitrogen by eating other organisms.

Answer:

B. It converts nitrogen into a form that humans can obtain by eating other organisms.

Explanation:

Right for e2020

What is chemical compound and why is it important to understand how the different elements are bonded together?

Answers

Explanation:

An element is a material that consists of a single type of atom. Each atom type contains the same number of protons. Chemical bonds link elements together to form more complex molecules called compounds. A compound consists of two or more types of elements held together by covalent or ionic bonds.

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why is graphing scientific data important?

Answers

It's important because it presents data that are either too numerous or complicated to describe. Hope this helped
Since most of the data scientist collect is quantitative, data tables and charts are usually used to organize the information • Graphs are created from data tables • They allow the investigator to get a visual image of the observations, which simplifies interpretation and drawing conclusions • Valid conclusions depen

Which biological scenario(s) best describes a rigid phenotype for gene expression?



I. Each person has a specific blood type.
II. Red blood cell counts differ in higher altitudes.
III. One identical twin is taller than the other.



1) I only


2) II only


3) II and III only


4) I, II, and III

Answers

Answer is 3 because it seems more logical

Explanation:

seems more logical

Answer:

1) I only

Explanation:

A person's blood type is more specific whereas one's red blood cell count and height varies.

Which of the following speed units is NOT from the Si (metric) system?*
cm/s
m/min
km/hr
mi/hr
m/s
km/min

Answers

Answer:

Prefixes are not usually used to indicate multiples of a second greater than 1; the non-SI units of minute, hour and day are used instead. On the other hand, prefixes are used for multiples of the non-SI unit of volume, the litre (l, L) such as millilitres (ml).

Knowing that the genetic code is universal, scientists use molecular biological methods to insert the human beta-globin gene into bacterial cells, hoping the cells will express it and synthesize functional beta-globin protein. Instead, the protein produced was nonfunctional and is found to contain many fewer amino acids than does beta-globin produced by eukaryotic cells. Explain why.

Answers

Answer is given below :

Explanation:

When the beta-globin gene is inserted into the bacterial chromosome, during insertion, beta-globin forms a stop codon between the coding sequences of beta globin, resulting in a reduced amino peptide. Acid on translationFurthermore beta-globins form a tertiary protein complex with alpha-globin, which in addition to the ham-subgroup forms functional hemoglobin. Since we only put the beta-globin gene on the bacterium-chromosome that is not enough to make a functional protein, we found a non-functional protein

Severe storms all have extremely strong winds that can cause a lot of environmental damage.
Which storm below has the strongest winds?
O cyclone
O hurricane
O thunderstorm
O tornado

Answers

The storm that has the strongest winds is TORNADO.

A tornado can be defined as a narrow-violent rotating column of air in contact with the ground, which is often attached to the base of a thunderstorm.

It is hard to observe a tornado unless it forms a condensation vertical wind tunnel composed of water droplets and dust.

In the most violent tornadoes, wind speed may reach 300 miles (480 kilometers) per hour.

In conclusion, the storm that has the strongest winds is TORNADO.

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Answer: its tornado i just took the test on edge2020  on the storm assignment.

Explanation: :)

Need some quick help with this:

Answers

Answer:

Explanation:

D

Answer:

option C

Explanation:

cellular respiration is the correct answer of this question.

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A women has blood type o, her baby has type a: is it possible that a man who has the blood type ab could be the father?

Answers

Yes the guy could be the father

Answer: Yes it could

Explanation: type o is the type that can be donated to anyone and it is a rare kind and it's also dominated the father type ab is a type where it's not as dominant but it can take over there for the baby could be type a cause type o is rare to get.

8. In snapdragons, red flower (R) color is incompletely dominant over white flower (1) color. The heterozygous (Rr)
plants have pink flowers.
A. If a red-flowered plant is crossed with a white-flowered plant, what are the genotypes and phenotypes
of the plants F1 generation?
B. What genotypes and phenotypes will be produced in the F2 generation?
C. What kinds of offspring can be produced if a red-flowered plant is crossed with a pink-flowered plant?
D. What kind of offspring is/are produced if a pink-flowered plant is crossed with a white-flowered plant?

Answers

Answer:

A. Genotypes: Rr

Phenotypes: All Pink

B. Genotypes in the F2 generation: RR, 2Rr, rr

Phenotypes: red, pink and white

C. 1RR (red): 1Rr (pink).

D. 1Rr (red) : 1rr (white).

Explanation:

This question involves one gene coding for flower color in snapdragon plants. The allele for red color (R) is incompletely dominant over the allele for white color (W), to form an intermediate pink color (RW).

A. If a red-flowered plant (RR) is crossed with a white-flowered plant (rr), the genotypes of the offsprings will be Rr with a pink color phenotype.

B. In the F2 generation i.e. Rr × Rr, the following will be produced: RR (red), 2Rr (pink), and rr (white).

C. if a red-flowered plant is crossed with a pink-flowered plant, the following will be produced: 1RR (red): 1Rr (pink).

D. if a pink-flowered plant is crossed with a white-flowered plant, the following will be produced: 1Rr (red) : 1rr (white).

gizmos Student Exploration: Photosynthesis Lab answer key pleaseeeee

Answers

Gizmo student is psychologically chemically imbalanced and a brain surgeon

EMB agar is a medium used in the identification and isolation of pathogenic bacteria. It contains digested meat proteins as a source of organic nutrients. Two indicator dyes, eosin, and methylene blue inhibit the growth of gram-positive bacteria and distinguish between lactose fermenting and non-lactose fermenting organisms. Lactose fermenters form a metallic green or deep purple colonies, whereas the non-lactose fermenters form completely colorless colonies. EMB agar is an example of which of the following?
a. a selective medium onlyb. a differential medium onlyc. a selective medium and a chemically defined mediumd. a selective medium, a differential medium, and a complex medium

Answers

Answer:

a selective medium, a differential medium, and a complex medium

Explanation:

EMB Agar (Eosin Methylene Blue Agar) is both a selective and differential culture medium that slightly inhibits Gram-positive bacteria and can be used to distinguish bacteria capable of fermenting lactose from those that are unable to ferment this disaccharide. This medium is commonly used for isolation and differentiation of coliforms (i.e., bacteria found in the soil and water samples) and fecal coliforms (bacteria found in the gut and feces samples). In EMB agar, lactose-fermenting-gram-negative bacteria (e.g., E. coli) will form colored colonies, while non-lactose-fermenting-gram-negative bacteria will form colorless colonies (e.g., Salmonella). EMB agar is composed of lactose, gelatin, methylene blue dye and eosin Y dye ( a xanthene dye) .

In RNA processing, the coding sequence that remains
in the final mRNA.
A. exons
B. operon
C. genome
D. diploid
E. nucleosome
F. recessive
G. restriction enzyme
H. haploid
I. dominant
J. gel electrophoresis

Answers

Answer:

The answer is A. exons.

Explanation:

Exons-In RNA processing, the coding sequence that remains in the final mRNA.

Explain why autotroph is used to describe producers

Answers

Answer:  An autotroph is an organism that can produce its own food using light, water, carbon dioxide, or other chemicals. Because autotrophs produce their own food, they are sometimes called producers. Most autotrophs use a process called photosynthesis to make their food.

Explanation:

Why are fossils not found in igneous rocks?

Igneous rocks are too dark in color to contain fossils.
Igneous rocks are found too deep underground.
Igneous rocks are made from cooling of lava or magma.
Igneous rocks are too dense to contain fossils.

Answers

Answer:Igneous rocks are made from cooling of lava or magma.

The Big Five personality factors are five ranges of key personality traits. Describe each of the five factors. Then, describe how stable the traits are and how heritable they are.

Answers

Answer:

The big five traits are:

1- extraversion

2- neuroticism

3- agreeableness

4- openness

5- conscientiousness

Explanation:

The Big Five personality traits, also called the OCEAN model, is a widely accepted theory in psychology that states that personality can be reduced into 5 major factors: extraversion, agreeableness, openness, neuroticism and conscientiousness. Agreeableness refers to the manner in which one person treats other people. Conscientiousness refers to the ability to control impulses. Extraversion refers to the sociability level. Openness refers to the willingness for new experiences. Neuroticism refers to the person's emotional stability. These five traits are relatively stable during lifetime. Moreover, it has been shown that the Big Five traits are affected by both genetic factors (family) and cultural factors (education) in a proportion of 50:50. In consequence, approximately half of the differences observed between individuals result from genetic inheritance and half from environment.


What happens during Go phase of the cell cycle?
O A. The cell splits in two.
B. The cell starts to die.
O c. The cell continues to live.
D. The cell makes new chromosomes.

Answers

Explanation:

The answer is C. because this is a new cell that is not preparing to divide or die..

I give you brilliant

Answers

Answer:

Adenine and Guanine

Explanation:

These two bases are included in the 4 types of nitrogenous bases. Which are the two purine bases (which are Adenine and Guanine)

True or False: The vast majority of creatures that have ever lived are not
preserved in the fossil record.*
O True
O False

Answers

Answer:

true

Explanation:

Why do animals become adapted to their environments?

Answers

Answer:

For survival

Explanation:

All organisms need to adapt to their habitat to be able to survive. This means adapting to be able to survive the climatic conditions of the ecosystem, predators, and other species that compete for the same food and space.

Hope this helps:)

Select the correct answer.
Which of these activities increases the amount of carbon in the atmosphere?

Answers

Burning of fossil fuel

Ecologists are interested in quantifying different kinds of biodiversity. In each of the two studies outlined here, which kind of biodiversity are ecologists studying?

a. Ecologists draw blood samples from basking shark (Cetorhinus maximus) specimens caught from a single large population in the Atlantic Ocean. From each blood sample, the ecologists isolate DNA. Comparing several DNA sequences across the individuals that were sampled, the ecologists are able to estimate the variation in DNA sequences in the entire population.

b. Ecologists establish 20 small areas, called plots, randomly scattered across a California grassland. They count the number of different plant species present in each plot. By noting how many species appear in the plots, the ecologists are able to estimate the number of species in the entire grassland.

Answers

Answer:

The correct answer is -

A. Genetic diversity.

B. Species diversity

Explanation:

In the first study, ecologists are studying the DNA sequences of the basking sharks and comparing the DNA they collected from the blood sample for finding the variation in DNA. The study of DNA and comparing the variations of DNA of a species is studying the genetic diversity of the species.

In the second study, the ecologists tried to study the different types of species present in small patches or plants, they created small patches of land, it is the study of species diversity.

What are Hydrothermal Vents? Do they contribute to the saltiness of the sea? Why or why not?​

Answers

Answer:

These vents contribute dissolved minerals to the oceans, which is one reason the oceans are salty. Rivers and surface runoff are not the only source of dissolved salts. Hydrothermal vents are recently-discovered features on the crest of oceanic ridges that contribute dissolved minerals to the oceans.

Hydrothermal vents form at locations where seawater meets magma

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Which pair of phenomena both support the particle model of light?
A. The photoelectric effect and constructive interference
B. Diffraction and interference
C. Blackbody radiation and diffraction
O
D. Blackbody radiation and the photoelectric effect

Answers

Answer:

D. Blackbody radiation and the photoelectric effect

How many teaspoons does it take to get 9 table spoons?


a. 21
b. 14
c. 27
d. 18

Answers

Answer:

c. 27

Explanation:

each tablespoon is equals 3 teaspoons

3×9=27

Answer:

27

Explanation:

3 teaspoons equal 1 tablespoon. So 9 times 3 =27

Dendrite
Cell body
Myelin sheath
Axon
Terminal branches
This cell is representative of the
system.
:: nervous
:: muscular
5 skeletal
* endocrine

Answers

Answer is Nervous system I think
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