In a diploid cell, a FISH probe should produce ____________ signals in metaphase FISH and ____________ signals in interphase FISH.

Answers

Answer 1

Answer: Four, two

Explanation:

FISH (fluorescence in situ hybridization) is a technique for chromosome mapping with probes that emit fluorescence and allow the visualization, distinction and study of chromosomes of nuclei in metaphase or inferophase. In this way, any type of anomaly such as inversions, aneuploidies, duplications or microdeletions can be seen. To do this, a non-specific DNA staining is performed with DAPI (a fluorophore that marks the nucleus where DNA is present) and the fluorescence will be seen under the microscope.

When cells are in metaphase, their chromosomes are condensed in preparation for cell division. To stop them in metaphase, colchicine is used which is a microtubule depolymerizing agent responsible for separating the sister chromatids of a chromosome, thus preventing the progression of cell division and arresting the cell cycle in metaphase. Here we will see translocations, microdeletions or duplications.

Interphase FISH is used to see decondensed chromatin, which allows higher resolution in detecting small abnormalities. It is mainly used for the detection of aneuploidies or large deletions, duplications or translocations when samples are difficult to grow or are scarce, as in the case of fetal or tumor cells. It is not possible to distinguish between a normal karyotype and a karyotype showing a balanced translocation. In addition, it can be used in the analysis of solid tumors, which divide very infrequently.

During metaphase, the chromosomes are duplicated so there would be two sets of chromosomes (a total of four chromosomes). Then, there would be four signals for each probe. During interphase, the chromosomes are not dupicated. Since it is a diploid organisms, there would be two chromosomes and this will represen two signals for probe.


Related Questions

What is the purpose of a geological time scale ?

It used to predict natural disaters throughout Earth’s history.
It is used to present the correct sequence of events in the Earth’s history.
It is used to determine the absolute dates in years for different periods.
It used to create a naming system for flora and fauna.

Answers

Answer: B. It is used to present the correct sequence of events in the Earth’s history.

Explanation: On Edge!!!! :)

Answer:bbbbb

Explanation:

qcw3ec

The brain generally stores new information in its own different and unique netowrk but retrieves information by chunking it with information in netowkrs that contain similar characteristics or associations.

a. True
b. False

Answers

A would be the correct answer I believe

Light dependent reactions are carried out both on and in between photosystems. This process is like the last stage of aerobic respiration in that both:______.
a. reaction sequences carry out electron transfer phosphorylations.
b. processes generate ATP.
c. processes involve electron flow.
d. systems are lodged along and within a membrane surface.
e. all of the above.

Answers

Answer:

e. all of the above

Explanation:

Photosynthesis and respiration are two complementary processes.

Photosynthesis occurs in chloroplasts. The electron transport chain is placed in the thylakoid membrane, where it occurs light-dependent reactions.Respiration occurs in mitochondria, in the internal membrane, where is the electron transport chain.

Photosynthesis needs solar radiation to produce organic compounds.The respiration process needs organic compounds to obtain chemical energy.

Photosynthesis transforms CO₂ into organic compoundsRespiration transforms organic compounds into CO₂.

In the photosynthesis process occurs a release of oxygen, which is used during the respiration process.  Both processes are part of the carbon biological cycle.Both processes use electron transport chains to capture energy, which will be used in other cellular reactions.

Respiration´s final products are CO₂, ATP, and waterPhotosynthesis´s final products of light-dependent reactions are oxygen, ATP, and NADPH.  

In pointers dogs, the Punnett square for tail length is below (gene A). Use the Punnett Square below to determine the possible offspring from a cross between two heterozygotes.
F1
A1 A2
A1 A1 A1 A1 A2
Never born Bow-tie tails
A2 A1 A2 A2 A2
Bow-tie tails Long tails
a. In terms of tail length what kind of inheritance does this gene show?
b. In terms of survival what kind of inheritance does this gene show?

Answers

Answer:

a) Autosomal dominant inheritance ⇒ The presence of at least one A1 allele is enough to express Bow-tie tails.

b) Autosomal recessive inheritance ⇒ The presence of at least one recessive allele A2 is enough for individuals to survive.

Explanation:

Due to technical problems, you will find the complete explanation in the attached files.

which molecules in your body do not contain carbon atoms

Answers

Inorganic compounds essential to human functioning include water, salts, acids, and bases. These compounds are inorganic; that is, they do not contain both hydrogen and carbon.

explain how misuse of human resources can be harmful

Answers

Using too many Human Resources can cause burnout, as well as leave less resources for those who genuinely need them.

According to a famous article by Max Kleiber, the scaling of the metabolic rate or energy consumption for mammals Pmetab (measured in kcal/day) with the body mass Mb (measured in kilograms) is Pmetab=70M0.75b.
a. What is the specific metabolic rate, i.e. the metabolic rate per kilogram of body mass for the following animals?a mouse whose mass is 30g. Give your answer to three significant figures.
b. What is the specific metabolic rate, i.e. the metabolic rate per kilogram of body mass for the following animals?a cow whose mass is 300kg. Give your answer to three significant figures.

Answers

Answer:

Explanation:

Given that:

The scaling of the specific metabolic rate is:

[tex]=\dfrac{P_{metab}}{m}[/tex]

where;

[tex]P_{metab} = 70M_b^{0.75}[/tex]

[tex]=\dfrac{ 70M_b^{0.75}}{m}[/tex]

(a) For a mouse whose mass = 30 g = 0.030 kg

The specific metabolic rate [tex]=\dfrac{ 70*(0.030)^{0.75}}{(0.030)}[/tex]

= 168.197 kg

≅ 168 kg to 3 significant fig.

(a) For a cow whose mass = 300 kg

The specific metabolic rate [tex]=\dfrac{ 70*(300)^{0.75}}{(300)}[/tex]

= 16.8197 kg

≅ 16.8 kg to 3 significant fig.

which structures are found in typical prokaryotic cells and also in typical plant cells
a) cell walls
b) histones
c) telomeres
d) tonoplasts​

Answers

Answer:

a. cell wall

pls Mark brainliest

Help me out with this again, pretty please?

(2nd time)

I need explanation for your answers, even though it's multiple choices, I still need your explanation for it.

DUE TOMORROW!

If your answer is NONSENSE it will be deleted as soon as possible!

But if your answer is CORRECT, HELPFUL, HAS AN EXPLANATION, I'll chose your answer as the BRAINLIEST ANSWER!​

Answers

Answer:

A. atomic number ✅A. groups ✅

Hope this helps!!

The membrane potential that occurs when neurotransmitters bind to their receptors is called _______.

Answers

Answer:

action potential

Explanation:

Transmission of a signal within a neuron (from dendrite to axon terminal) is carried by a brief reversal of the resting membrane potential called an action potential. When neurotransmitter molecules bind to receptors located on a neuron's dendrites, ion channels open.

Answer:

I guess action potential is the correct one

Albinism, lack of pigmentation in humans, results from an autosomal recessive gene. Two parents with normal pigmentation have an albino child. What is the probability that their next three children will be wild type

Answers

Answer:

50

Explanation:

because this disorder occurs when the alleles are recessive meaning the alleles Will be heterozygous

How are meiosis and mitosis similar?



A.They both produce diploids.

B.They happen at the same time.

C.They both produce sex cells.

D.They both produce new cells.

Answers

Answer:

D.They both produce new cells.

Explanation:

Meiosis and mitosis are both processes of cell division; however, they have many differences. Meiosis occurs in sex cells and produces haploids that can be fertilized. Mitosis occurs in body cells and produces diploids that replace old or damaged cells. Still, both processes result in the creation of new cells.

Answer:

D) They both produce new cells!

Explanation:

Dont forget to give a thank you, and to rate my answer!

What is added to tacos, burritos, and soups that is
high in fiber?

Answers

Answer: flax seed whole wheat tortillas.

1⁄2 cup low-fat refried beans.

1⁄4 cup low-fat cheddar cheese.

leaves lettuce.

1⁄ cup salsa.

1⁄4 cup fat free sour cream.

Pinto beans are added to tacos, burritos, and soups that are high in fiber.

What are fibers and their types?

The body is unable to digest fiber, which is a form of carbohydrate. While fiber cannot be converted into glucose, the sugar that results from the breakdown of most carbs, fiber travels through the body undigested.

The solubility, viscosity, and fermentability of dietary fibers, which have an impact on how they are metabolized by the body, can be used to classify them in general. Dietary fibers have a wide range of chemical compositions.

Fibre comes in two varieties: soluble and insoluble. Both are crucial for good health, digestion, and illness prevention. Thus, Pinto beans are added to tacos, burritos, and soups that are high in fiber.

Learn more about fibers, here:

https://brainly.com/question/13805719

#SPJ6

Which of the following is correctly defined?

A. Ecosystem - A group of conspecific organisms.

B. Population - An individual life form.

C. Community - A group of populations.

D. Population - A group of communities.
I need help!!1

Answers

Answer:

both b and c I think..........

Population - An individual life form. and Community - A group of populations.

What is population?

A population is the entire set of people in a group, whether that group is a country or a collection of people who share a certain trait.

A population is the group of people from which a statistical sample is taken in statistics. Therefore, a population is any collection of people who have something in common.

A statistically significant portion of a population, rather than the full population, may also be referred to as a sample. For this reason, the standard deviation, or standard error, of a statistical study of a sample from the full population must be disclosed.

Therefore, Population - An individual life form. and Community - A group of populations.

To learn more about population, refer to the link:

https://brainly.com/question/27991860

#SPJ2

How is it possible for mad made things to move?

Answers

How is it possible for mad made things to move?Answer: Because the human body is designed to use their muscles to create and transfer things from place to place, and that some man-made objects are light enough to be moved

Which of the following statements regarding membrane protein function is false? Group of answer choices Membrane proteins act as receptors to molecules like hormones. Membrane proteins serve as enzymes. Membrane proteins form junctions between cells. Membrane proteins transfer genetic information to the cytoplasm.

Answers

Answer:  

Membrane proteins transfer genetic information to the cytoplasm.

Explanation:

Membrane proteins cannot transfer genetic information to the cytoplasm. Genetic information is only in DNA and RNA, and to pass it, RNA has to leave the nucleus and be translated into a protein in the cytoplasm. Once the protein synthesis has finished, the protein may go to the membrane if it has the signal to do it or have a different function. In the membrane, the membrane protein can receive information from other cells and transfer it to the cytoplasm, but never in the shape of genetic information.

How does penicillin kill bacteria? Penicillin augments the body's natural ability to enzymatically degrade bacterial peptidoglycan, causing the cells to lyse Penicillin targets a cross-linking reaction in cell wall manufacture. By blocking the enzyme responsible, penicillin stops the bacteria from producing new peptidoglycan. Penicillin targets muramic acid in the cell wall. By damaging a key component of the cross-linking reaction, the cell can't build a strong peptidoglycan mesh and quickly breaks apart. Penicillin damages the lipid membrane of bacterial cells by enzymatically removing peptidoglycan from the membrane's outer surface. The weakened cells quickly lyse.

Answers

Answer:

The correct answer is option c. By blocking the enzyme responsible, penicillin stops the bacteria from producing new peptidoglycan.

Explanation:

Penicillins are derived from Penicillium molds. These are a group of antibiotics, used in treating various types of bacterial infections such as urinary tract infections, respiratory tract infections, gastrointestinal infections etc.

These antibiotics also used in treating gonorrhoea, meningitis, pneumonia, syphilis, etc. These antibiotics stop the bacteria from producing new peptidoglycan that blocks the enzyme.

how myelinated and nonmyelinated neurons differentiated?

Answers

Answer:

The main difference between these two types of neurons is the speed of conduction of impulse. ... Majority of the neurons in the central and peripheral nervous system are myelinated since they require fast conduction speeds. A neuron with unmyelinated axon has a comparatively lower speed of conduction of the nerve signals.

Explanation:

_______and_______process help in balancing of carbon dioxide and oxygen in the environment​

Answers

Answer: Respiration and combustion

atoms May bond together to make larger what ​

Answers

Atoms bond together to make larger molecules or elements.

Human belongs to Class of?

Answers

I believe the answer is Mammal . I hope this helps

8. Two rabbits are bred together. One is heterozygous for agouti coat
(genotype: Cch) and the other is albino. What are the possible phenotypes
of their offspring? Construct a Punnett square to support your answer.

Answers

Answer:

- Parental cross = Cch  x chch  

- F1 = 1/2 Cch (agouti coat); 1/2 chch (albino coat) >> 1:1 phenotypic ratio

Punnett  square:  

          ch         ch

C       Cch       Cch

ch    chch      chch

 

Explanation:

A heterozygous individual is an individual who has two different gene variants (i.e., alleles) at a particular locus. In this case, individuals having the "agouti coat" trait are heterozygous carrying both 'C' and 'ch' alleles. On the other hand, a homo-zygous individual has the same allele at a given locus (here, the 'chch' genotype associated with the albino phenotype). Therefore, as observed in the Punnett Square above, when a heterozygous parent is crossed with a homo-zygous recessive parent for a single gene, alleles segregate in the gametes of both parents so an expected 1:1 phenotypic ratio will be observed.

3 Peroxisomes are cellular organelles: A. with their own genome B. present only in protists C. without membrane OR D. collaborating with the nucleus O E site of some oxidation reactions

Answers

Answer:

E.site of some oxidation reactions

Should we clone animals that are going or have gone extinct (in other words bring them back
either from the brink of extinction or from extinction)? Explain your answer.

Answers

No, God gives and takes away, the world is heartless and kills entire species to extinction but it’s nothing that God didn’t already know would happen. Dinosaurs are no longer here there extinct for a reason, nowadays extinction comes from man but everything that happens in this world is Already written out in Gods book

Answer:

I think we should but people are preventing this though

Explanation:

I think we should because it might benefit the world and let scientists to discover this animal. But then I don't think so because animals that are/were extinct could perhaps be from climate change. Nowadays, people don't take this matter seriously causing habitats to be destroyed and animals to be extinct :) Like icebergs are melting that mean penguins are at risk of being extinct,if we did something then this situation will be prevented. However dinosaurs did become extinct and they did not come back so its probably just life and this is how god planned it:)

Which of the following would NOT be a reliable source of information?

Answers

Eyewitness.... because it can't actually sometimes determine what really happened on the site so it can't give a reliable sitrep

Las semillas de las plantas, especialmente las más consumidas en nuestra dieta como el trigo, el maíz, el arroz y otros cereales o las lentejas, los porotos y otras legumbres, tienen un depósito de almidón que está destinado a alimentar el embrión en las primeras etapas del desarrollo, hasta que esté en condiciones de hacer la fotosíntesis por sí solo. ¿Con qué función de los lípidos se relaciona esta adaptación? Justificar.

Answers

Answer:

lol I dont know the answer

Question 8 of 24
Which item is a carbon sink?
A. Beach sand
B. Ocean water
C. Glaciers
D. Forest fires
SUBMIT

Answers

Answer:

oceans water

Explanation:

Which of the following is a benefit of dams?

A. They impede fish migration and spawning

B. They provide a source of energy

C. They increase the sediment load of waterways


D. They divert water from ecosystems

Answers

A. They impede fish migration and spawning

The _______ is the part of the neuron that carries information away from the cell body.

Answers

mitochondria is the part of the neuron that carried information away from the cell body

A newly discovered pigmented microbe contains a capsule and flagella, but no chloroplasts or cilia. This microbe is approximately 2 microns in size, and divides by binary fission. The nucleic acid of this microbe is packaged within an envelope. Based on this information, is this microbe a eukaryote or a prokaryote? Explain the reasoning behind your answer.

Answers

Answer:

in which grade did u read

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