I need help. What is a cloning vector? How are cloning vectors used? Give three examples of cloning vectors, indicating which ones are better for small fragments of DNA and which are better for large fragments.

Answers

Answer 1

Answer:

1. A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.

2. Cloning vectors are plasmids that are used primarily to propagate DNA. They replicate in E. coli to high copy numbers and contain a multiple cloning site

3a. Examples of cloning vectors are plasmids, cosmids, bacterial artificial chromosomes (BACs), and yeast artificial chromosomes (YACs).

3b. plasmids- small, BACs- large, YACs- large, cosmids- large

Explanation:

hope this helps

Answer 2

A cloning vector is a short piece of DNA which can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.

What is Cloning Vectors?

A cloning vector is defined as a short piece of DNA that can be stably maintained in an organism, and into which an external DNA fragment can be inserted for cloning purposes. This may be DNA taken from a virus, the cell of a higher organism, or it may be a plasmid of a bacterium.

Cloning vectors are plasmids that are mainly used to amplify DNA which replicate at high copy number in E. coli and contain a multiple cloning site.

Examples of cloning vectors are plasmids, cosmids, bacterial artificial chromosomes (BACs), and yeast artificial chromosomes (YACs).

Thus, a cloning vector is a short piece of DNA which can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.

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Related Questions

In one or two sentences, explain how the chemical equation for cellular respiration shows that plants cycle matter. Include the numbers of molecules and atoms involved to support your explanation.

Answers

Carbon dioxide + Water Glucose (sugar) + Oxygen CO2 + H2O C6H12O6 + 6O2 Cellular respiration or aerobic respiration is a series of chemical reactions which begin with the reactants of sugar in the presence of oxygen to produce carbon dioxide and water as waste products.

Glucose + Oxygen ------> ATP + Carbon dioxide + Water

C6H12O6 + 6O2 ----------> 36ATP + CO2 + H2O

The chemical equation for cellular respiration shows that plants cycle matter because oxygen and glucose that is produced by the plants is used by the human body for the generation of energy in the form of ATP molecules, water and carbondioxide gas.

This carbondioxide gas is released by animals which is used by the plants in order to make their own food during the process of photosynthesis so we can conclude that the chemical equation for cellular respiration shows that the plants cycle matter.

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2. The researchers in the film spent a month collecting and identifying various species of animals and plants. These species were then added to a database of all species in Gorongosa. Provide two (2) reasons why building a species database will be useful for the restoration project.

Answers

Answer:

Importance of building specie database, for restoration projects.

Explanation:

Restoration implies human intervention, for renewing - degraded ecosystems & habitats.

Building species data base is important for this, as - wildlife is an integral part of habitat. Understanding their favourable living ambience in ecosystem - like temperature, humidity, sunlight & water access and migration patterns is important - in effort to restore environment in real sense.

If a gene has two alleles, and allele A has a frequency of 83 percent, then allele a has a frequency of:_______

Answers

Answer:

Another allele frequency = 17%

Explanation:

Given:

Allele A has a frequency = 83 percent

Find:

Another allele frequency

Computation:

Sum of percent = 100%

So,

Another allele frequency = 100% - 83%

Another allele frequency = 17%

Which layer of soil contains large rocks that have not been weathered yet?
A. Topsoil
B. Dirt
C. Bedrock

Answers

Answer: Option C.

Bedrock

Explanation:

Bedrock consist of hard rock that has not been weathered because bedrock is the hard rock that is found under surface materials like soil and are components of quartzite, limestone , basalt and granite. It is a solid and it is bound very tight. It also form parent rock and it can remain on the top of the soil which later weathered and form soil and can be taken away by wind, water, gravity e.t.c.

Is hemoglobin a hormone?

Answers

Answer:

I'm really sorry it is not.

A new hormone called erythroferrone, which regulates the iron supply needed for red blood-cell production, has been discovered by researchers. Iron is an essential functional component of hemoglobin.

Hemoglobin is the iron-containing protein compound within red blood cells that carries oxygen throughout the body. It is made up of heme, which is the iron-containing portion, and globin chains, which are proteins. The globin protein consists of chains of amino acids, the "building blocks" of proteins.

Answer:

no its not

Explanation:

The first heart sound is made by

blood moving to the atria
blood moving to the ventricles
opening of the valve between the atria and the ventricles
closing of the valve between the atria and the ventricles

Answers

Closure of the valve (mitral valve and tricuspid valve)

What does the picture present?
Chromosome
Nucleas
Gene​

Answers

Answer:

Chromosome

Explanation:

8. How does the circulatory system function?
A. Receives and interprets messages.
B. Moves and performs simple body activities.
C. Removes salts and minerals from the blood.
D. Transports blood, water and nutrients to all parts of the body.​

Answers

Answer:

d. transports blood, water and nutrients to all parts of the body.​

Explanation:

A population of the Galapagos finch Geospiza fortis undergoes directional selection for larger bill size after Geospiza fuliginosa colonizes the same island. This is an example of:

Answers

Answer: character displacement

Explanation:

Character displacement is an evolutionary change which happens in a scenario whereby two identical species live in same environment. When this occurs, natural selection takes place which then favors a divergence based on the characters that the organisms possess.

Therefore, when a population of the Galapagos finch Geospiza fortis undergoes directional selection for larger bill size after Geospiza fuliginosa colonizes the same island is an example of character displacement.

choose the correct food chain that contains 5 trophic levels

Answers

Answer:

i think its the first one. someone fact check me in the comments.

What are the most reactive elements of the periodic table

Answers

Answer:

The reactivity of alkali metals increases from the top to the bottom of the group, so lithium (Li) is the least reactive alkali metal and francium (Fr) is the most reactive.

2. Chancroid presents with a genital ulceration that is a) Painless b) Gummata in nature c) Purulent lesion d) Painful

Answers

D

i

am

right

so

trust

me

i

swear

on

my

mama

this

is

correct

my

g

what are the first atoms that were formed?

hydrogen and helium

photons and neutrons

iron and lead

protons and neutrons​

Answers

hydrogen and helium were the FIRST atoms, I hope this helps you.

Which property could be used to identify a substance?

A. mass
B. weight
C. density
D. volume

Answers

c. density because no matter the size of the substance the density will always be the same

Most igneous activity takes place?

Answers

Mid-Ocean Ridges it’s because they are mostly produced near the “ring of fire” cause the ridges are volcanically active!

Answer:

under the ground in volcanoes

Explanation:

There are some volcanically active spots that are in the middle of a tectonic plate.

The interior of the Earth is hot, while the surface or crust of the planet is cold. Between these layers are areas of molten rock. These molten rocks are less dense, so they easily break through cracks in the Earth's crust.

Magma is molten rock and is found beneath Korea. Beneath the volcano, in the underground spaces, magma accumulates.

do all animal cells have same genetic content(DNA)?!... if so why are the cells grouped into tissues?​

Answers

Answer:

Becasue there are different type of cells within the body that function different ways.

Explanation:

A graph of KE vs the mass of an object is ___

Answers

Answer:

A non linear relationship

Explanation:

hope this helps.

A graph of KE vs the mass of an object is a non-linear relationship.

What are graphs?

Graphs are the mathematical representations of numbers and data on graph paper.  They are an easy way to see the changes in the data over time.  

In nonlinear relationships, changes in any of the inputs are not proportionate to changes in the output. A nonlinear relationship produces a curve when it is plotted on a graph, whereas a linear relationship results in a straight line.

On a graph, kinetic energy has a linear relationship with respect to the mass of the moving object and a non-linear relationship with respect to how fast the object is moving.          

Thus, the graph of KE will be a non-linear relationship.    

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Which word best describes the tone of the excerpt?

A. mocking
B. frustrated
C. mysterious
D. troubled

Answers

Answer:

C Mysterious

Explanation:

We don't know the full infermation about the story.

Answer:

c

Explanation:

PLSSSSSSSSSSSSSSS HELP!!!!!!!!!!!! FIRST TO AWNSER GETS BEAINLIEST!!!!!!!!!!!!!!!!!

Prepare a public service announcement for each illness in which you describe 1) how the illness is transmitted, 2) how it is treated, and 3) any prevention measures that are being used to prevent the illness. Include a bibliography with each announcement to show the names of the sources you used. You may create your announcement in written form as a pamphlet, poster, or computer slideshow. Alternatively, you may make your announcement in the form of a video or audio recording.

Answers

Answer:

D

Explanation:

Roller coasters, with their twists, turns, and loops, seem to defy everything we know about how objects move. Roller coaster designers use Newton's laws to
push people past their usual limits. Which statement identifies Newton's Law of Motion with the correct justification of that law? (DOK 2, AKS 8b)
OA Newton's first law is the law of inertia. Since an object at rest stays at rest, all roller coasters have to be pushed or pulled to get started.
OB. Newton's first law is the law of force and acceleration. Since the rollercoaster is so massive, it will require more force to accelerate
OC. Newton's third law of action-reaction states that for every action, there is an equal and opposite reaction. This means that as you push down on the
seat, the seat does not push back on you with an equal force.
OD. Newton's second law is the law of force and acceleration. Since the rollercoaster is so massive, it will require less force to accelerate.

Answers

Answer:

The answer is D

Explanation:

and can you pls post a lil more points

pls thanks

____keep most populations in their natural habitat at a population size somewhere between extinction and overrunning the ecosystem.
PLEASE HELP!!

Answers

Answer:

limiting factors

Explanation:

The ecological optimum represents the value of the factors within the ecological valence at which the life processes of a species take place best. The upper and lower limits of an ecological valence are its maximum and minimum. Beyond these limits, physiological processes are interrupted and death occurs.

What do cells do with the products produced by cellular respiration?
A.
Carbon dioxide and water can be excreted and the energy is used by the cell.
B.
Glucose and carbon dioxide can be excreted and the energy is used by the cell.
C.
Carbon dioxide and oxygen can be excreted and the energy is used by the cell.
D.
Glucose and oxygen can be excreted and the energy is used by the cell.

Answers

Answer:

Ok

Explanation:

I believe the answer is D

Answer:

A

Explanation:

Carbon dioxide and water can be excreted and the energy is used by the cell

What is the end result of mitosis?
a. Two new cells with identical sets of chromosomes
b. Two new cells with different sets of chromosomes
c. One cell with two identical sets of chromosomes
d. One cell with two different sets of chromosomes

Answers

The answer is A) Two new cells with identical sets of chromosomes

Any one know plz help ty

Answers

I believe it is A!

the punnet square would be:

B B
b Bb Bb

b Bb Bb


and since capital letters represent dominance and black is dominant, it would be 4 black guinea pigs!

In what way do two alleles for the some trait differ?


A. The are found on different chromosomes.

B. They consist of different sequences of DNA

C. They are produced by two different people.

D. They are complementary.

Answers

Answer:

B. They consist of different sequences of DNA

Explanation:

Alleles are contrasting or alternative forms of a gene. A diploid organism i.e an organism with two sets of homologous chromosome, contains two alleles for each gene that encodes a trait. Although the two alleles of that particular gene encodes the same trait, they can encode different phenotypes i.e way it is expressed because they contain different sequences of DNA.

For example, in a gene coding for height in pea plants, two alleles, T and t are responsible. Allele T codes for tall height (phenotype) while allele t codes for short height (phenotype). Although the two alleles are for the same trait, they differ in the content of their DNA sequence, hence, are expressed differently or variably.

This enzyme digests proteins into their basic building blocks called _________

Answers

the answer is proteases

Answer:

enzyme pepsin

Explanation:

What type of biomolecule are enzymes?
Carbohydrates
Proteins
Lipids
Nucleic acid

Answers

Answer:

protein

Explanation:

................

Geologists can use sedimentary rock to learn about climate in the past.

True
False

Answers

answer:
true



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Answer:True

Explanation:

28. Which statement best explains why cellular respiration in plants and
other organisms is dependent on photosynthesis?
Photosynthesis is one of the final steps in cellular respiration,
Photosynthesis absorbs materials that are catalyzed during cellular respiration.
Photosynthesis absorbs excess energy produced by cellular respiration.
Photosynthesis provides the materials that fuel cellular respiration.

Answers

Answer: Photosynthesis provides the materials that fuel cellular respiration.

Explanation:

Photosynthesis, the process through which green plants convert light to chemical energy. The energy that is gotten from sunlight by green plants, some protistans, and bacteria is used to produce sugar which is then converted to ATP by cellular respiration which is regarded to as the fuel that living things use.

Therefore, the statement that best explains why cellular respiration in plants and other organisms is dependent on photosynthesis is that photosynthesis provides the materials that fuel cellular respiration.

Answer:

Photosynthesis provides the materials that fuel cellular respiration.

Explanation:

Which of the following is not true of sexual reproduction?
A. Sexual reproduction involves gametes
B. Sexual reproduction involves the simple division of a cell
C. Through sexual reproduction, traits and characteristics of the species are passed on to offspring
D. Most multicellular organisms reproduce through sexual reproduction

Answers

Answer:d

Explanation:

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