How will you isolate DNA from a pure fungal culture

Answers

Answer 1

Answer:

Explanation:

There are several ways of isolating DNA from a pure fungal culture but you can use the CTAB method.

In this method, genomic DNA can be extracted.

First of all, the cell walls of fungal mycelia can first be broken down by grinding it using glass rods or you can use or liquid nitrogen. Place it in a plate. You can now CTAB extraction buffer , then incubate at 65°C, then let it undergo purification with phenol:chloroform:isoamyl alcohol 25: 24: 1 and carry out precipitation with isopropanol . Then the DNA can be extracted and then dissolved in 50 μl of pure water.


Related Questions

g Would an observational study or an experiment be more appropriate to investigate the effects on fertilizer on plant growth? Explain.

Answers

Answer:

The economists typically receive information premised upon the information gathered during controlled studies. A further explanation is provided below.

Explanation:

The investigators typically add particular manipulation throughout the observational investigation and analyze the consequences.The survey was performed here because the investigators simply would be required to examine the influence of fertilizer on the development of plants. Each assistance must be applied.

Aspirin may still irritate the stomach as a side effect. What functional group in the molecule is responsible for this effect

Answers

Answer:

The functional group that irritates the stomach is known as the salicylic acid molecule.

Explanation:

What is the Ukrainian?

Answers

Explanation:

Ukraine is a country in Eastern Europe. It is the second-largest country in Europe after Russia, which it borders to the east and north-east.

hope it helpsstay safe healthy and happy....

explain the importance of taking named precautions before blood transfussion can be done?​

Answers

Answer:

To minimize the chance of an adverse reaction during a transfusion, health care practitioners take several precautions. Before starting the transfusion, usually a few hours or even a few days beforehand, the person is cross-matched with the donor blood (not done for transfusions of plasma or platelets).

Precautions are necessary for blood transfusion because
There are chances of getting exposed to fatal diseases like hepatitis, AIDS and many other diseases.
And avoid the reactions between antigen and antibodies, which leads to rejection of transfused blood.

Good luck

Regards
BLACKSHARK

Answer this question properly​

Answers

Answer:

Hindi ko alam hehee

Explanation:

ssorry po

A reproductive cell must differ from other normal body cells. The reproductive cell must ______________ because it will soon fuse with another reproductive cell to form a new individual

Answers

I'm not 100% sure what the exact answer is to this, because lots of things could be said about what gametes need to do for reproduction.

I suppose since it says that it's combining with another cell, it's hinting at the fact that reproductive cells are haploid.

If this is multiple choice I would choose the choice that has to do with gametes being haploid.

A reproductive cell must differ from other normal body. The reproductive cell must Haploid because it will soon fuse with another reproductive cell to form a new individual.

How is a reproductive cell specialized?

The requires the union of two specialized cells, called gametes, each of which contains one set of chromosomes.

Thus, haploid is the  answer.

To learn more about haploid click here:

https://brainly.com/question/7212380

In the Science Olympiad, what is the main branch of science to study?

Answers

There is no main branch of science to study. The changing nature of genetics, earth science, chemistry, anatomy, physics, geology, mechanical engineering and technology are equally important in the Science Olympiad.

Answer:

Well, so far there are three branches of Science namely;

Earth SciencePhysical ScienceLife Science

Describe a metabolic pathway that is inhibited by the action of a pesticide.

Answers

Explanation:

The mecanism is termed as feedback inhibition.

Explanation:

In an enzyme catalyzed sequential reaction the end product of that reaction inhibits the activity of the activity of the enzyme catalyzing the ist step This type of  regulation is called Feed back regulation or feedback inhibition.

              The main function of human body is to maintain the homeostasis of the all the components it contains.When the end product of an ongoing enzyme catalzsed sequential reaction is produced in a high lebel at that time to maintain its own homeostatis that end product inhibits the functioning of the enzyme catalzsing the ist step of that biochemical reaction.

For Example Cytidine tri phosphate inhibits the activity of aspartate transcarbamoylase catalyzing the de novo biosynthesis pathway of pyrimidine metabolism.

Which of these is a benefit of fish farming?

A. It can deplete native fish populations


B. It can restock lakes depleted by recreational fishing


C. It can pollute natural bodies of water


D. It can pass diseases to native fish populations

Answers

Answer:

The answer to this would be B

Explanation:

B:It can restock lakes depleted by recreational fishing

Mutations that result in the death of the individual are known as

Answers

Answer:

Mutations that cause the death of an organism are called lethals — and it doesn't get more negative than that.

Classify each item as being associated with light positioning (controlling how and where light strikes the retina) or sensory processing (how that information is processed).

a. Photoreceptors
b. Occipital cortex
c. Extrinsic muscles
d. Pupil
e. Bipolar cells

Answers

Answer:

- light positioning: c. Extrinsic muscles; d. Pupil

- sensory processing: a. Photoreceptors; b. Occipital cortex; e. Bipolar cells

Explanation:

Extrinsic (extraocular) muscles are muscles that modulate both eye movement and position, whereas intrinsic (intraocular) muscles are muscles that focus the eye and control how much light enters into the eye. The pupil is the black center of the eye within the iris through which light passes before being focused onto the retina. Photoreceptors are specialized neurons located in the retina that respond to light, converting it into electrical signals that can stimulate biological processes. Photoreceptors in the retina are divided into two categories 1-rods (responsible for vision at low light levels), and 2-cones (active at higher light). Bipolar cells are neurons that transmit signals from the photoreceptors to the ganglion cells, thereby processing light signals received from rods and cones. The occipital cortex is a brain region that represents the visual processing center and contains most of the anatomical region of the visual cortex.

The genetic code is defined degenerate or even redundant because:
A. the same codon codes for different amino acids
B. an amino acid can be encoded by several codons
C is different in all organisms, except in monozygotic twins
D. the sequence of codons is not separated by intervals, but is continuous
E. the structure of the genes is constantly mutating

Answers

Answer:

B. an amino acid can be encoded by several codons

Explanation:

The genetic code refers to the set of rules that govern how the four nitrogenous bases (A,U,G,C) is arranged to form amino acids. The genetic code is a collection of all the CODONS formed. There are different characteristics of the genetic code and one of them is its REDUNDANCY.

Since there are 4 nitrogenous bases combined in three's to form a codon i.e. 4³, there are 64 possible codons that could form 20 maximum amino acids. This means that there are more codons in relation to amino acids. Hence, more than one codon can encode an amino acid, and this is referred to as REDUNDANCY of the genetic code.

For example, LEUCINE amino acid is encoded by 6 codons as follows: UUA, UUG, CUU, CUC, CUA, CUG.

Discuss how important you think it is to protect ecosystems from human-induced change, such as changes in habitat or introduction of invasive species. Should natural conditions be preserved at all costs, should human needs always prevail, or is the answer somewhere in between? Discuss the negative consequences of taking an approach that you disagree with.

Answers

Answer:

Humans impact the physical environment in many ways: overpopulation, pollution, burning fossil fuels, and deforestation. Changes like these have triggered climate change, soil erosion, poor air quality, and undrinkable water.  ecosystems are vital for human welfare and survival, as they provide us with essential products and benefits.

Over 90% of our food comes from terrestrial ecosystems, which also provide energy, building materials, clothes, medicines, fresh and clean water, and clean air. Protecting wildlife and preserving it for future generations also means that the animals we love don't become a distant memory. And we can maintain a healthy and functional ecosystem.

Which of the following events is typical of meiosis but not of mitosis?
A the chromosomes are highlighted
B. the centrioles are formed
C chromatids separate
D. the homologous chromosomes are paired AND the spindle is formed

Answers

Answer:

D. the homologous chromosomes are paired AND the spindle is formed

Explanation:

Meiosis is the type of cell division that results in four daughter cells with each having a reduced number of chromosomes (by half). Both meiosis and mitosis occur in different stages including prophase, metaphase, anaphase etc but meiosis occurs in two series of division processes called meiosis I and meiosis II.

In prophase I of meiosis, homologous chromosomes, which are similar but non-identical chromosomes from male and female parents pair to form tetrad or bivalent. This pairing of homologous chromosome is unique and peculiar to MEIOSIS but never mitosis.

What’s the main reason why cell specialization is important

Answers

Explanation:

because we know that cell makes up organ and organ makes organ system and this system makes organism....cell is also the structural and functional unit of life it there were no cell specialization then mitosis and meosis wont take place neither cell division

I need help asap
I need help asap​

Answers

Answer:

c

Explanation:

i dont care

In one study, 108 regions of the genome were identified to be significantly associated with schizophrenia. Below is a Manhattan plot showing the results from this study. Assuming that none of the causative variants are linked and there is a single causative variant per peak region, how many regions of chromosome 6 with causative variants were identified in this study

Answers

Answer: hello your question is incomplete attached below is the missing part of the complete question

The top of the highest peak corresponds to a region of the genome called the MHC locus. This means that of all the variants identified by this study, ____________.

the variant in MHC is observed the most frequently in the affected cohort.  

the variant in MHC can cause schizophrenia in a Mendelian fashion.

the variant in MHC is the least likely to be a false positive result.

the variant in MHC has the largest odds ratio.

answer:

i) 4

ii) the variant in MHC is the least likely to be a false positive result. ( C )

Explanation:

The number of regions of chromosome 6 with causative variants identified is 4

While the variant in MHC is the least likely to be a false positive result. this is because, it is the highest peak in of all the causative variants identified in this study (i.e. closely related to the disease)  and been the highest peak will make it least likely to return a false positive result.

Which non-mineral nutrient is essential for photosynthesis? ANSWER ASAP!!!!!!!!
A.hydrogen
B.potassium
C.nitrogen
D. carbon dioxide

Answers

Answer:

A. hydrogen

Nearly all organic compounds also contain H atoms, which explains why plants need the H they get from water molecules through photosynthesis. Hydrogen ions are vital in both aiding proton gradients to help drive the electron transport chain in photosynthesis, and for plant respiration.

#CarryOnLearning

Answer:

carbon dioxide

Explanation:

because carbon dioxide is needed for respiration

Try this for yourself. Suppose you want to estimate the number of field mice in a small field. One day you capture 500 mice, mark all of them and release them. The next week you capture 200 mice of which 40 were marked by you yesterday. What is your estimate of the population size?

Answers

Answer:

The approximate size of the field mice population is 2500 individuals.

Explanation:

There are different methods to study population density. There are direct methods and indirect methods. Among these last ones, we might find the "Mark-Recapture technique".

The Mark-Recapture technique assumes that the population is closed,  during the sampling season there is no mortality nor natality, marks in the individual last the whole sampling season, and these do not affect the marked individual or their behavior. These marked individuals are randomly distributed in the population, and all the individuals have the same probability of being sampled.

The method consists of capturing a sample of individuals belonging to the population under study, in this case, the field mice population. After capturing the individuals, the researcher marks them and release them again. The third step is to sample again, capture a new amount of individuals, and count how many of them are marked. These marked individuals belong to the first sample. We can calculate the population size by using the Lincoln Index or any other index.

The Lincolns Index states that to obtain the population size, the research must follow the following procedure:

1) Take an initial sample with n1 number of individuals

2) Mark all the sampled individuals and release them

3) Take a second sample with n2 number of individuals

4) Count the number of marked individuals m

To estimate the total number of individuals in this population, N, the following equation must be considered:

N = (n1 x n2)/m

In the exposed example, this is:

• n1= 500 individuals in the first sample

• n2= 200 individuals in the second sample

• m= 40 individuals in the second sample that were previously marked  

             as they were part of the first sample    

N = (n1 x n2)/m = (500 x 200) / 40 = 2500 individuals

The approximate size of the field mice population is 2500 individuals.  

Genes a and b are 10 map units apart, b and c are 20 map units apart, and a and c are 30 map units apart. If a triple heterozygote is testcrossed, among 1,000 progeny, how many are expected to result from double crossovers if there is no interference?
а. 10;
b. 20;
c. can't be determined
d. 30;
e. 60;

Answers

Answer:

20 ( B )

Explanation:

Given data:

a and b are 10 map units apart

b and c are 20 map units apart

a and c = 30 map units apart

condition ; Triple heterozygote testcrossed

number of progeny = 1000

Determine the number of double crossover result

P( crossover between a and b ) = 10/100 = 0.1

P( crossover between b and c ) = 20/100 = 0.2

p( double crossover ) = 0.1 * 0.2 = 0.02

hence number of double crossovers = number of progeny * 0.02

                                                              = 1000 * 0.02 = 20

Normally, this region is imprinted in the sperm, but not the egg. This means that the DNA on the ____________ copy of the chromosome will be methylated at the imprinting center, while the DNA on the ____________copy of the chromosome will not be methylated in this region.

Answers

Answer: The DNA on the paternal copy of the chromosome will be methylated at the imprinting center, while the DNA on the maternal copy of the chromosome will not be methylated in this region.

Explanation:

Chromosomes are DNA structures associated with proteins such as histones. They are found in the nucleus of cells and contain genetic information for the development and functioning of an organism. Human beings are diploid, meaning that they possess two copies of each of the 23 chromosomes (a total of 46).

Gametes, which are sex cells such as the sperm (produced by the male) and the egg (produced by the female) that are haploid. This means that they possess only one chromosome of each pair. During fertilization, a male gamete fuses with a female gamete to generate a zygote, which will give rise to a new human being. This new individual will possess half of the genetic material from its father and half from its mother. Thus having a total of 46 chromosomes, 23 from each parent.

Genetic imprinting modulates gene expression by chemical modification of DNA and/or modification of chromatin structure. Often, genetic imprinting causes a gene to be expressed only on the chromosome inherited from one of the parents. One example of imprinting is DNA methylation, which is a process by which methyl groups are added to DNA. Methylation modifies DNA function when found in the promoter gene, repressing gene transcription. This means that a methylated gene will not be expressed, that is, it will not produce a protein encoded by that gene. So, if a region of DNA is imprinted in the sperm cell, the paternal chromosome inherited from this sperm will be methylated in the genes of the offspring. And the offspring will only express the maternal copy inherited, which will not be methylated.


6. What does the concept of learning set suggest?
O A. Ideas are needed to solve difficult problems.
O B. Emotion needs to be excluded from experiments involving cognition.
C. Imagination is the key to problem solving.
D. Animals learn how to learn.

Answers

Answer:

B. Emotion needs to be excluded from experiments involving cognition.

Explanation:

#CarryOnLearning

[tex]{hope it helps}}[/tex]

PLEASE BE QUICK!!!!! This scientist is using a computer model to predict the
weather.
What is one limitation of computer models?
A. Computer models help scientists make predictions about complex
systems
B. Computer models analyze many pieces of data in a short time.
ООО
C.
mputer models require valid data to make accurate predictions.
D. Computer models test hypotheses by simulating different
situations.
PREVIOUS

Answers

Answer:

C

Explanation:

I would say C because it HAS to have valid data. Which is a limitation especially if you can't get that data.

Complete each sentence by dragging each word or phrase to the proper position. Then arrange the sentences into the order of occurrence of motor impulses of the ANS.
1. Action potentials travel along the nerve fiber and enter the__________.
2. Fibers exiting the take one of three routes: the spinal nerve route, the sympathetic nerve route, and the _________nerve route.
3. Arriving at target cells_______ nerve fibers release acetylcholine or norepinephrine into the synaptic cleft.
4. Also known as the________ division, the sympathetic nervous system exits the preganglionic CNS via the spinal nerves from T1-L2.
5. The effect of norepinephrine binding to_______ receptors stimulates cardiac muscle cells. i. postganglionic
ii. sympathetic chain ganglia,
iii. craniosacral
iv. thoracolumbar
v. cholinergic
vi. preganglionic
vii. adrenergic
viii. splanchnic

Answers

Answer:

1. vi. Preganglionic

2. vii. adrenergic

3. ii. sympathetic chain ganglia

4. iii. Craniosacral

5. viii. splanchnic

Explanation:

Action potential travel down preganglionic nerve fibre. They travel towards Vagus Nerve near the effector organ. These impulses arrive at cardiac plexus, which create action potential in postganlianic.

A cell that connects the body to receive the signal from the brain to do the action is called a neuron and the tissue system is called the nervous system.

The answer to the following question is as follows:-

Action potentials travel along with the nerve fiber and enter the Preganglionic Fibers exiting take one of three routes: the spinal nerve route, the sympathetic nerve route, and the adrenergic nerve route. Arriving at target cells sympathetic chain ganglia nerve fibers release acetylcholine or norepinephrine into the synaptic cleft. Also known as the Craniosacral division, the sympathetic nervous system exits the preganglionic CNS via the spinal nerves from T1-L2.The effect of norepinephrine binding to splanchnic receptors stimulates cardiac muscle cells.

Hence, these are the answer to the question.

For more information, refer to the link:-

https://brainly.com/question/3523174

02-Why is it important to meet the members Of your extended family?​

Answers

Answer:

because they are our heart and supporting members

Upon completion of seeding a flower and a vegetable, research and identify the optimum propagation conditions, such as, water and light demand, appropriate temperature, and, other elements that may affect the germination from seeding. Compare your research findings with your existing conditions. If any discrepancies found, address how you would like to mitigate. Describe the entire process in your weekly lab report journal. Add photos, sketches or other supplementary information, if necessary.

Answers

Answer:

Needs optimum environmental conditions.

Explanation:

Seedling, a flower and a vegetable needs suitable conditions for growth and development. All of the three stages of plant life cycle requires optimum water, fertilizers and light intensity in order to grow and develop at full speed. Seeds need a specific temperature in order to germinate. Most seeds germinate when the soil temperature is between 68 and 86 Fahrenheit. In the same way the plant also needs specific temperature for bearing flowers. All the stages requires optimum water in the soil for absorption.

Write the events of the human life cycle in the correct order, starting with what happens right after a pair of gametes comes together?

Answers

Answer: Fertilization occurs, a zygote undergoes mitosis, a baby's cells divide mitotically, meiosis produces sex cells that will participate in fertilization.

Explanation:

Incomplete questions, options are:

Meiotic cell division occurs as the zygote develops into a gamete, meiosis produce the sex cells that will participate in fertilization, a baby grows into an adult through uncountable meiotic divisions, fertilization occurs, a zygote undergoes mitosis, a baby's cells divide mitotically, mitosis produces gametes.

1. Fertilization occurs: Fertilization is the union of a human egg and sperm (male and female gametes), which usually occurs in the fallopian tube. The result is the formation of a zygote or fertilized egg, which initiates the development of the embryo. In this way, the genetic material of both gametes is also fused.

2. A zygote undergoes mitosis: The zygote is the union of the egg and the sperm. Each gamete contributes 23 chromosomes to form the genetic material of the future organism. The zygote starts to divide throught mitosis. Mitosis is a type of cell division that generates two genetically identical daughter cells and occurs in somatic cells (all cells in the body that are not gametes). Eventually, it divides into two blastomeres, then into 4, then into 8, and so on during the passage of the zygote along the fallopian tube towards the uterus. Subsequent divisions of the 12 cells give rise to the morula. Then, the blastocyst is generated, which is an embryo of between 5-7 days of development and is composed of a large number of cells that begin to differentiate.

3. A baby's cells divide mitotically: An embryo is a living being in the early stages of its development, from fertilization until the organism acquires the morphological characteristics of the species. In the specific case of the human being, the term applies until the eighth week after conception (fertilization). Cell divisions for development occur by mitosis. In this way, the embryo will grow and develop, forming tissues and organs through growth thanks to mitotic divisions.

4. Meiosis produces sex cells that will participate in fertilization: Meiosis is a type of cell division that occurs in the gonads for the production of gametes, whereby a diploid cell undergoes two cell divisions, that generates four haploid cells. Thus sperm and oocytes are produced to eventually fuse during reproduction to form a diploid zygote. Meiosis has certain differences from mitosis, since in this type of cell division, the daughter cells created are all genetically distinct from each other and from the parent cell. This is because a crossing over occurs between non-sister chromatids of homologous chromosomes to exchange genetic material. This ensures that each gamete produced has genetic variability.

Earth science includes the study of all the following except: Select one: a. land. O b. water. O c. atmosphere. O d. wildlife. ​

Answers

Answer:

c. atmosphere

Explanation:

the atmosphere is not part of the Earth's surface

define cell and atom​

Answers

Answer:

cell is the structural and functional unit of live.

Atom are the smallest particle of chemical element

Which of the following was not part of the original cell theory, but was added later after we learned more about cellular structure?

A. all cells have the same basic composition

B. all cells come from pre-existing cells

C. all cells have a nucleus

D. all cells have the same exact structures.​

Answers

Answer:

c oooooooooooookkkkkkkkkkk

Other Questions
Step 3: Let LM = x. We know the lengths of the radii of each circle, so KL = 12 +8 = 20. Add the length of KL to the diagram.J12 K20LXM12008N The decrease in the intensity of light over distance, such as with increasing depth in water, is known as ______. Journalize Closing Entries Using the information from the Adjusted Trial Balance, journalize the closing entries for the end of the month. Date SMART TOUCH LEARNING Adjusted Trial Balance December 31, 2016 Accounts and Explanation Debit Credit Account Title Balance Debit Credit 19.800 Accounts Recewable 10.900 Date Office Supplies Accounts and Explanation 200 Debit Credit Prepaid Rent 13.200 Furniture 23.100 Acumulated Depreciation Accounts Payable 2.600 Salanes Payable 600 best Pay 200 Uneaned Re Notes able Date Accounts and Explanation Debit Credit Comment 12.000 Dividends 33.700 Serce Reven 50.000 Depression Expense Rumine Date Accounts and Explanation Debit Credit Interesten 300 Rent tense Slanes Expen 4.600 1,000 Total 113.300 SprayCo Inc. develops and produces spraying equipment for lawn maintenance and industrial uses. On March 9 of the current year, SprayCo reacquired 16,900 shares of its common stock at $23 per share. On June 9, 10,600 of the reacquired shares were sold at $25 per share, and on November 13, 4,100 of the reacquired shares were sold at $25.Required:Journalize the transactions of March 9, June 9, and November 13. Dan (driving his own car) picked up his friend Rodney to drive to their softball game. Both Dan and Rodney have a Personal Auto Policy (PAP) with $5,000 of medical payments coverage per person. Dan hit a parked car, and Rodney was injured, incurring $9,000 of medical expenses. How will this claim be settled under the other insurance provision of the PAP Punctuate I passed all of my final exams now I can prepare for graduation. nate and laura picked 20 apples laura picked 1/2 of what nate pickedhow many did nate pick? PLZZZZ HELPP WILL GIVE BRAINLIESTTwo sides and the non-included right angle of one right triangle are congruent to the corresponding parts of another right triangle. Which congruence theorem can be used to prove that the triangles are congruent?AASSSSSASHL Please answer of question num 20 and 21 only please Question 4 of 26 How are guyots and seamounts related? A. A guyot is a seamount that has a flat top because of wave action. B. A guyot forms when a seamount becomes completely surrounded by a coral reef. C. A seamount forms when a guyot becomes completely surrounded by a coral reef. D. A seamount is a guyot that has a flat top because of wave action. what are the rules in forming irregular nouns 8-64+10divided by 2 = which relation is a function? Weavers Inc., a popular clothing brand, has been providing its customers with the best quality silk products for many years.The employees of Weavers Inc.use pink berry silkworms for their silk products and weave their greased silk threads in such a way that they produce different shades of colors when held at different angles.Weavers Inc.has been providing unmatched service toward millions of customers around the world.Other companies have tried to imitate their products and services, but have not been successful.Few competitors who captured these silkworms did not know the precise temperatures that were essential for their breeding and survival.Which of the following factors of inimitability does this scenario regarding Weavers Inc.best reflect? A) Positive network externalities B) Tacit knowledge C) Time compression diseconomies D) Causal ambiguity The slope of the lines that passes through the points (-10,0) and (-13,3) a sample of unknown material weighs 500 n in air and 200 n when immesersed in alcholol with a specfic gravity of 0.7 what is the mass density In its recent income statement, Smith Software Inc. reported paying $12 million in dividends to common shareholders, and in its year-end balance sheet, Smith reported $386 million of retained earnings. The previous year, its balance sheet showed $372 million of retained earnings. What was the firm's net income during the most recent year one key aspect/influence of Jacksons presidency foiuhgfdknsmlaeszxcyfvugbihnoj Purchased goods for $4,100 from Diamond Inc. with terms 2/10, n/30. 5 Returned goods costing $1,100 to Diamond Inc. for credit on account. 6 Purchased goods from Club Corp. for $1,000 with terms 2/10, n/30. 11 Paid the balance owed to Diamond Inc. 22 Paid Club Corp. in full. Required: Assume that Ace uses a perpetual inventory system and that the company had no inventory on hand at the beginning of the month. Calculate the cost of inventory as of June 30.