The absorption spectrum of a molecule is independent of the excitation intensity because the absorption of light by a molecule is a quantized process and is determined solely by the molecule's energy levels.
The emission spectrum of a molecule is independent of the excitation wavelength because the molecule will always emit photons with energies corresponding to the energy difference between its excited and ground states.
The absorption spectrum of a molecule is determined by the energies of the electronic transitions that can take place in the molecule. These energies are fixed and depend only on the molecular structure and the electronic configuration of the molecule.
The intensity of the absorbed light is proportional to the number of molecules that undergo this transition, and not the intensity of the incoming light.
Similarly, the emission spectrum of a molecule is determined by the energy differences between the excited and ground states of the molecule. Once excited, the molecule will emit photons with energies corresponding to these energy differences, regardless of the excitation wavelength used to excite the molecule.
In a fluorescence microscope, a fluorophore (a molecule that can absorb and emit light) is used to label specific molecules in a sample. When excited with light of a certain wavelength, the fluorophore emits light of a different wavelength, which can be detected and used to form an image.
The independence of absorption and emission spectra from excitation intensity and wavelength ensures accurate labeling and detection of the fluorophore.
DNA is the genetic material that contains genes, which are segments of DNA that encode specific proteins through the process of transcription. Fluorescence in situ hybridization (FISH) is a technique used to visualize specific DNA sequences in cells. Labeling either an intron or an exon of a gene can help identify the location and expression level of that gene.
DAPI and Hoechst are both fluorescent dyes that can bind to DNA and be used for DNA visualization in microscopy. DAPI has higher DNA specificity and less background staining, while Hoechst is less toxic and can penetrate cell membranes more easily.
The Molecular Expressions website provides detailed information on the basics of fluorescence microscopy, including the principles of fluorescence, the components of a fluorescence microscope, and various fluorescence techniques used in microscopy.
For more questions like Light click the link below:
https://brainly.com/question/10709323
#SPJ11
How do antibiotics work? Note: you will not be given credit for simply stating “they prevent bacterial growth” or “they kill bacteria”
Answer:
here's your answer
Explanation:
May this helps you..
How is the brain involved with the senses and what is the relationship to the wat a personreacts to objects?
Answer:
The senses are the components of our nervous system that capture stimuli from the environment for transmission to the brain, and it is there where the information is processed to issue a response. All sensations are electrical signals that travel through neurons which form a network that communicates all organs with the center of the nervous system, and communicate with each other to transmit the electrical impulse through a process known as synapsis.
Explanation:
The brain is an organ that controls the activity of the body, both its conscious and unconscious functions. It is located in the head and corresponds, therefore, to the encephalon of humans and other vertebrates and is subdivided into forebrain, midbrain and hindbrain.
The brain acts on the rest of the organism by generating muscle activity or by the production and secretion of chemicals called hormones. Thereby the brain controls responses to changes in the environment. Some basic types of response such as reflexes may be mediated by the spinal cord or peripheral ganglia, but sophisticated intentional control of behavior based on complex sensory information requires the integration of information.
The thalamus, which is located in the central part of the brain, processes and coordinates the messages from the senses that it receives from the body. Five senses are considered to exist: sight, touch, smell, taste and hearing. For that, the information perceived from the outside must reach the brain.
The senses are the components of our nervous system that capture stimuli from the environment for transmission to the brain, and it is there where the information is processed to issue a response. Therefore, all sensations perceived from the outside are electrical signals that travel through neurons. For example, the eyes transform light signals into electrical impulses, which travel to the brain where they are transformed into electrical signals.
Neurons form a network that communicates all organs with the center of the nervous system, and communicate with each other to transmit the electrical impulse through a process known as synapsis, which is mediated by molecules called neurotransmitters. Since the electrical impulse cannot jump directly from one neuron to another, these neurotransmitters are needed. When the active neuron produces them, the next neuron in the network detects them and becomes electrically charged. Once this has happened, it produces neurotransmitters again so that the next one becomes electrically activated. This is how the electrical impulse reaches the brain.
Crocuses can bloom in the winter, while dahlias bloom in the fall. This is due to different responses to daylight length or _________.
A. Sun rays
B. Photoperiodism
C. phototropism
D. geotropism
Answer:
It's becoz of sun rays.....
What is the name of the site of transcription?
1. Amino acid
2. Nucleus
3. Cytoplasm
4. Mitochondria
It is proper to put the _____________ allele before a recessive allele when determining the genotype of the offspring in a Punnett square.
Answer:
dominant
Explanation:
the dominant allele is the capital letter (for example, T) whereas the recessive allele is the lowercase letter (for example, t). the dominant allele is usually written first.
It is proper to put the dominant allele before a recessive allele when determining the genotype of the offspring in a Punnett square.
What are alleles?Alleles are the variation present in the gene or DNA sequence. It is the sequence of nucleotides that encodes the production of the product of a gene.
There are two types of alleles, dominant and recessive, the dominant gene is denoted by a capital letter, and it is placed before the recessive allele.
Thus, in a Punnett square, the dominant allele should come before the recessive allele when determining the genotype of the offspring.
Learn more about alleles, here:
https://brainly.com/question/18075358
#SPJ2
Can someone help with this ?
Answer:
to convert ADP to ATP
= first option
Explanation:
so it must be the first option
since in order for this conversion the process stated in the first option must occur.
Necesito hacer un mapa mental sobre taxonomia y fuandamento filosofico como lo puedo hacer me ayudan plis es urgente
Answer:
A taxonomy map of philosophy is created using 5 layer version
Explanation:
The map is drawn in a circular form, the parent is usually the center and then the child or the sub branches are then made around the first- parent circle to show the relation between the parent and its child. These branches can have their own sub branches which are then included around the branches the child of the original parent.
How long will it take a cross country runner to cover a distance of 350 meters at a speed of 17.5 m/min?
Answer:
20 minutes
Explanation:
Divide distance by speed to get time
D/S = t
350/17.5 = 20
(Just an fyi you posted this in biology)
What would happen to the production of the high energy sugars if water or carbon dioxide were not
available to plants?
ATD
Answer:
There would be no production of high energy sugars in the plant.
Explanation:
Plants produce their food (autotrophism) in a process unique to them called PHOTOSYNTHESIS. Photosynthesis is the process whereby plants produce energy-storing sugars (glucose) in the presence of light from sun.
This photosynthetic process combines Carbon dioxide (CO2) and water (H2O) as reactants to form sugar (C6H12O6) and oxygen (O2) as products. The equation for photosynthesis is as follows:
6CO2 + 6H2O → C6H12O6 + 6O2
This means that water and carbon dioxide are strictly needed for the process of photosynthesis to occur in plants. Hence, if water or carbon dioxide were not available to plants, there would be no photosynthetic process and ultimately no production of high energy sugars.
Describe the two signals and pathways that are activated when you touch something and experience pain
I NEED HELP! Complete the table of the following element. Element: Chlorine Symbol: Cl
number of Protons:
Neutrons:
Electrons:
Atomic Number:
Atomic Mass:
Answer:
For example, the atomic mass of chlorine (Cl) is 35.45 amu because chlorine is composed of several isotopes, some (the majority) with an atomic mass of 35 amu (17 protons and 18 neutrons) and some with an atomic mass of 37 amu (17 protons and 20 neutrons).
Hope it helped!!!
What does semiconservative replication refer to?
a
Both strands are new in each new DNA molecule.
Both strands are conserved in each new DNA
molecule.
с
One strand is conserved and one strand is new in
each new DNA molecule.
d none of the above
Answer:
it is a
Explanation:
Both strands are new in each new DNA molecule.
3 questions
1. Water vapor needs ____ to become rain or snow
a. humidity
b. cool air
c. dust
d. all of the above
2. water droplets fall because they are too ?
a. warm
b. cool
c. heavy
d. cold
3. snow is formed when water droplets
a. form ice crystals
b. melt slightly
c. stick together
d. all of the above
These questions are worth 25 points first one to answer CORRECTLY gets marked brainliest :) good luck
Answer:
the answers are
1) b
2)c
3) a
Water vapor may be defined as the gaseous form of water that is usually dispersed across the atmosphere with the help of wind. This state of water is significantly found in the hydrosphere.
This gaseous form of water is formed from the evaporation or boiling of liquid water or from the sublimation of ice.
Water vapor requires to combine with dust particles and cool air along with atmospheric humidity in order to produce rain or snow. Due to the combination of such compounds, water droplets become too heavy.
Snow or ice is the solid appearance of water which is usually formed from the compaction of crystallized structure.
Therefore, each of the above questions is described.
To learn more about Water vapor, refer to the link:
https://brainly.com/question/11226635
#SPJ6
What happens during translation?
a. Messenger RNA is made from a DNA code.
b. The cell uses a messenger RNA code to
make proteins.
c. Transfer RNA is made from a messenger
RNA code.
d. Copies of DNA molecules are made.
Lack of medical knowledge
While exploring a rock formation, Hiroto finds a rock that has footprints pressed into it. A geologist tells Hiroto that the rock is millions of years old. Which of these claims
is correct about Hiroto's find?
It is not a fossil because footprints are not fossils.
It is not a fossil because only whole organisms are fossils.
It is a fossil only if Hiroto finds actual parts of the organism in rocks nearby.
It is a fossil because footprints of organisms from million of years ago are considered to
be fosss
Answer:
5 is the answer
Explanation:
Answer:
message me on ig
Explanation:
she.hatezay ill help you there
Precautions to take during potato osmosis lab
What effect does the deer's behavior have on the survival and reproduction of these two
types of cactus?
PLS HELP
Answer:
Greatly affected.
Explanation:
The deer's behavior has greatly affected the survival and reproduction of these two types of cactus because one cactus is eaten by deer while the other is saved from it. The cactus with no spines are eaten by deer which destroy the vegetative as well as reproductive parts of cactus so its survival and reproduction does not occur whereas the other cactus have spines on its body is save from deer and increase in the chances of survival and reproduction occur.
15 Which of the following mutations would have the potential to affect future
generations of a species?
A A frame shift mutation in the X chromosome of a cheek cell
B A chromosomal mutation in the Y chromosome of a kidney cell
CA point mutation in the first chromosome of a sperm cell
D A substitution mutation in the third chromosome of a uterus cell
Answer:
c i think
Explanation:
I don't know just trying to help hope you have a good day
7._________________________ lines your digestive tract and blood vessels. It moves food and blood through your body.
smooth muscle
skeletal muscle
cardiac muscle
Answer:
Smooth Muscle
Explanation:
In the digestive tract it's called the muscularis mucosa.
HELPP
The diagram below shows the similarities and differences of plants and animals complete the diagram by filling in the correct term as follows: heterotrophic and autotrophic and multicellular
Answer:
Both are multicellular (plants have different cells for the leaves and the stem, animals have skin cells, brain cells etc so they are called multicellular).
Plants are autotrophic - they make their own food (glucose) by photosynthesis
Animals are heterotrophic - they eat other organisms, cannot make their own food.
Explanation:
differentiate between thallophyta bryophyta and tracheophyta
Answer:
The main difference between Thallophyta Bryophyta and Pteridophyta is the organization of each phylum. Thallphyta consists of algae, fungi, lichens, and cyanobacteria. The plant body of Thallophyta is a thallus. ... The plant body of bryophytes is not differentiated into true stem, roots, and leaves.
Explanation:
please help me with this biology question.............
Why is the use of electron microscopy important in studying cells ??
Explanation:
Electronic microscope helps us to see each every part of the cell. So, it is considered important
Answer:
A cell is the smallest unit of life. Most cells are so small that they cannot be viewed with the naked eye. Therefore, scientists must use microscopes to study cells. Electron microscopes provide higher magnification, higher resolution, and more detail than light microscopes.
Explanation:
hope it's help you
Which of the following statements about viruses is true?
A: A virus belongs to the Archaea Domain.
B: A virus is a single-celled organism.
C: A virus has a cell membrane made of proteins.
D: A virus can evolve.
Answer:
D: A virus can evolve.
Explanation:
Viruses are not organisms so that rules out all other options.
Note: your teacher will review your response to this question to ensure you receive proper credit for your answer. The pH scale is a measurement system that indicates the concentration of _______ in solution.
please help
Answer:
hydrogen and hydroxide ions
Explanation:
The pH scale indicates the concentration of hydrogen ions (H+) and hydroxide ions (OH-) in a solution.
How many moles are present in 10, 0 grams of sodium hydroxide (NaOH )
Answer:0.250 mols
Explanation:
That’s the answer I got it wrong using the guy that previously wrote that the answer is 19 mols his totally wrong don’t trust him
We have that Moles that are present in 10, 0 grams of sodium hydroxide (NaOH ) is
Moles\ NaOH =0.25moles
From the question we are told
How many moles are present in 10, 0 grams of sodium hydroxide (NaOH )
Generally the equation for the Molar mass of sodium hydroxide is
[tex](NaOH) = (22.990 + 15.9994 + 1.008) \\\\ {(NaOH)} = 40 g/mol[/tex]
Where
Mass_{NaOH}taken = 10 g.
[tex]Moles\ NaOH =\frac{(mass of NaOH taken)}{(molar mass of NaOH)}\\\\Moles\ NaOH = \frac{10}{(40}\\\\Moles\ NaOH =0.25moles[/tex]
Therefore
Moles that are present in 10, 0 grams of sodium hydroxide (NaOH ) is
Moles\ NaOH =0.25moles
For more information on this visit
https://brainly.com/question/17756498
4. What domain name should replace the question mark in this chart?
5 points
Unicellular Protozoa
Uni/multicellular
algao
Multicollular
heterotroph
cell walls chitin
?
Multicoulluar
autotroph
cell walls
of cellulose
Multicellular
heterotroph
Lack cell walls
O a. Archaea
b. Bacteria
O c. Eukarya
O d. Monera
Answer:
C. Eukarya
Explanation:
Which of these structures do plants and algae have in common?
A. phloem
B. stems
C. chloroplasts
D. xylem
(pls answer this and have a good day)
Answer:
b and c
Explanation:
Which of the following has the highest reproductive potential?
O a. bacteria
O b. cattle
O c. humans
O d. elephants
Answer:
A. Bacteria
Explanation:
I asked my teacher hahahahahahahaha
Bacteria as the highest reproductive potential. Hence option a is correct.
What is reproductive potential?Reproductive potential is defined as the proportional ability of a species to reproduce itself under ideal circumstances. The maximum number of children that a particular organism can produce is known as its reproductive potential. Compared to other species, some have substantially higher reproductive capacity.
When people reproduce more frequently, sooner in life, and at higher rates, their reproductive potential rises. The largest influence on reproductive capacity is early pregnancy. Every living thing must be able to reproduce. All living things produce additional organisms that resemble them.
Thus, bacteria as the highest reproductive potential. Hence option a is correct.
To learn more about reproductive potential, refer to the link below:
https://brainly.com/question/28276770
#SPJ6
Please help!!!! Grizzly bears and polar bears are very closely related, so much so that they can reproduce to form hybrid offspring. Use your understanding of natural selection to describe how polar bears became a separate classification from the grizzly.
Scientists believe that at first these bears scavenged seal carcasses that had washed ashore, and gradually began to hunt the seals by waiting at the water's edge as the seals surfaced to breathe. This is believed to be an important step in the evolution of a new bear species, the polar bear
Over time the new polar bears were used to the cold climate and learnt how to fish, they then separated from the grizzly bears, they developed new creatures which would help them survive in one of the most dangerous climate.
Polar bears become separate classification from the grizzly by the natural selection known as divergent and adaptive evolutionary change.
Polar bears and grizzly bears are considered as closed to one another so they can interbreed. They both diverged from one another due to the harsh climate of the Arctic and the ecology of the region.
The changes that took place are camouflaging and pigment-free fur-coat that helped them in order to adapt to the different climates.The genetic mutation leads to such Adaptive changes when the polar bears migrated northwards.The populations living in different climates undergo natural selection that is divergent natural selection and the adaptive evolutionary changes that result in different species.Learn more about speciation:
https://brainly.com/question/4493180