Determine the correct sequence of events during the creation of recombinant DNA. I. Plasmid is cut open with enzymes II. Target gene is cut out with enzymes III. Plasmid is inserted into the bacterium IV. Target gene is bonded to the plasmid with Ligase

A. IV, III, I, II
B. II, I, IV, III
C. IV, I, II, III
D. I, II, III, IV

Answers

Answer 1

Answer:

The correct answer should be choice B if I'm going about this the right way.

Answer 2

The correct sequence is as follows:

II. Target gene is cut out with enzymes

I. Plasmid is cut open with enzymes

IV. Target gene is bonded to the plasmid with Ligase

III. Plasmid is inserted into the bacterium

Genetic engineering, also known as genetic manipulation, is the use of recombinant DNA to change the genetic makeup of organisms. In the creation of a recombinant DNA, which is usually a plasmid + desired gene, the following processes are employed:

1. The target or desired gene is initially cut out using enzymes called restriction enzymes.

2. The plasmid (extrachromosomal DNA) from a bacterium is then cut open.

3. Ligase enzyme is used to bond the target gene or gene of interest with the plasmid.

4. After bonding, the plasmid is inserted into a bacterium to multiply.

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Related Questions

Hi can someone please correct me.

Answers

I think it’s mountains also !

In diploid species, diploid cells may undergo either mitosis or meiosis. Haploid cells may undergo mitosis (for certain species) but NOT meiosis because

Answers

Answer:

The correct answer would be - Homologous chromosomes cannot pair

Explanation:

Haploid cells are the cells that have single set of chromosome or have the equal number of chromosome as their parent cells. Haploid cells are denoted by the n. Haploid cells are produced by the meiosis and are identical copies of their parent cells,

Haploid cells may undergo mitosis but not meiosis as their are already n number of chromosome and homologous chromosomes are can not pair and meiosis is a reduction division that produces haploid cell from diploid cells.

Thus, the correct answer is - Homologous chromosomes cannot pair

Which strategies did you include in your response about diabetes prevention? Check all that apply.

Answers

Answer: My strats about the diabetes prev. is very inclusive about most of their fat obesity problem so i would take them to a surgent to get checked out and later on tested for the diabetes lvls

Explanation:

because even if they stopped eating sugar or candy they would be extremely addicted, still which would be a big change for them and their mindset.

The picture below shows the setup for an experiment involving a 1000 ml beaker, sitting on a bumer, filled with 500 ml of water, where
the bumer represents the Sun's energy, and the beaker of water represents Earth's oceans.
1000 L
500 m
Bumer
During the experiment, black pepper was added to the water as it boiled strongly, and the pepper moved in a circular pattern from the top
of the water to the bottom and back again. What does the circular pattern of the pepper represent?

Answers

The circular pattern of the pepper represents the convention currents that exists in boiling water as it is heated.

What are convection currents?

A process that involves the transfer of energy from one location to another is a convection current.

Convection currents are caused by variations in fluid density brought on by temperature gradients. A natural convection current is the phenomenon that arises from the constant replacement of the heated fluid in the vicinity of the heat source by the adjacent present cooler fluid. Natural convection heat and mass transfer refer to the improved heat and mass transmission caused by this natural convection current.

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Debido a que las células de los hongos están cubiertos por una pared celular estos no tienen la capacidad de incorporar moléculas grandes y otros organismos sino que deben ingerir su alimento antes de absorberlo este tipo de digestión se denomina A. Intracelular B. Extracelular C. Quimica D. Mecanica

Answers

Answer:

B

Explanation:

Extracellular digestion is a form in which lager molecular food substances are first digested by chemical activities of enzymes and acids  outside the cells,before been absorbed into the body cells.

Fungi are examples of organism with this mode of digestion. They use external source of  energy to breakdown the food substances before digestion.They are called heterotrophic organism because they can not manufacture their food ,but rather depend on external source of energy derived from the organic matter from the  host they feed live on,

The energy sources are the carbohydrates and proteins from the host.

Can you Help me on this

Answers

Answer:

hypothesis=In science, a hypothesis is an idea or explanation that you then test through study and experimentation. Outside science, a theory or guess can also be called a hypothesis. A hypothesis is something more than a wild guess but less than a well-established theory. ... Anyone who uses the word hypothesis is making a guess.

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experiment=An experiment is a procedure carried out to support, refute, or validate a hypothesis. ... Uses of experiments vary considerably between the natural and human sciences. Experiments typically include controls, which are designed to minimize the effects of variables other than the single independent variable.

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observation=an act or instance of viewing or noting a fact or occurrence for some scientific or other special purpose: the observation of blood pressure under stress. the information or record secured by such an act. something that is learned in the course of observing things: My observation is that such clouds mean a storm.

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evidence=noun. that which tends to prove or disprove something; ground for belief; proof. ... data presented to a court or jury in proof of the facts in issue and which may include the testimony of witnesses, records, documents, or objects.

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4 benefits to liquid ink are

Ballpoint. Ink Base: Oil. Pigment or Dye: Pigment. Ink Properties: ...

Drawing (Dip Pen) Ink Base: Water. Pigment or Dye: Dye. Ink Properties: ...

Fountain. Ink Base: Water. Pigment or Dye: Dye. ...

Rollerball. Ink Base: Water. Pigment or Dye: Both. ...

Gel. Ink Base: Water. Pigment or Dye: Pigment.

Create 3
questions that
could be
answered using
the data shown in
the bar graph. Be
sure to number
your questions:

Answers

Answer:

1.what are the differences in each bar graph

2.what does each bar graph represent

3.in what time period did the Forrest change

which of the following is a property of water that allows for it to be easily attaracted to solid materials such as glass

a. cohesion

b. Adhesion

c. Good solvent

d. Structural support

Answers

adhesion allows for it to be easily attracted to glass

What is the basic unit of structure and function in living things

Answers

Cell

It is the basic structural and functional unit of live.

Every organism is made up of the things tiny cell

Answer:

Cell

Hope it helps ;)

Select the correct answer. Which type of tissue performs the role of signal conduction in the body? A. connective tissue B. epithelial tissue C. muscle tissue D. nervous tissue

Answers

Answer:D. nervous tissue

Explanation:

Which statement about nuclear fission is correct? produces very little energy produces a lot of air pollution requires large amounts of matter produces tremendous amounts of energy

Answers

Answer:

produces tremendous amounts of energy

Explanation:

Nuclear fission involves the split of the nucleus of high energy radioactive elements to produce a smaller nuclei with a high amount of energy which is often used to generate electricity.

It is also a very clean source of energy due to its inability to produce carbon compounds which are mostly associated with air pollution. It also doesn’t involve a large amount of matter.


I want to design an experiment to see if drinking coffee makes students
score higher on tests. What is the independent variable in this
experiment?

Answers

Answer: students drinking coffee

Explanation:

The dependent variable is the one that DEPENDS on what is about to happen. In this situation, whether the students scored higher DEPENDS on if they drank coffee.

So the independent variable would be the fact that they drank coffee.

Hope this helps!

The independent variable will be the scores

Which of the following is NOT a way a data table can hep you in
an experiment?
A. Collect the data
B. Interpret the data
C. Make your experiment more fun
D. Organize the data collected

Answers

Answer:

A.collect the data

what is a heart made of it

Answers

Answer:

Explanation:

he heart is composed of cardiac muscle, specialized conductive tissue, valves, blood vessels and connective tissue. Cardiac muscle, the myocardium, consists of cross-striated muscle cells, cardiomyocytes, with one centrally placed nucleus.

Shyam being very inquisitive, didn’t listen to the teacher’s instruction properly. He took the thin layer of onion peel with a drop of water on the glass slide. He placed the coverslip on it and started observing the slide under a microscope. But he was unable to see the cells of onion peel properly.

Q. What do you think he might have forgotten?
PLS GIVE THE ANSWER FAST I WILL MARK YOU BRAINLIST

Answers

Answer:

he forgot to adjust the brightness and the curtain. Slowly move the knob so that the objective goes up. Continue until the image is okay

Pretty clear focus. Then use the knob, if available, for a clearer picture. If you are on a microscope with a moving specimen carrier, turn the coarse knob so that the table carrying the specimen moves down or away from the objective. This is exactly how to use a microscope focus regulator.

Explanation:

What habitat is home to 50% of animal species, 70% of plant species, and is currently being destroyed by humans?

Answers

Answer:

Unique Biodiversity. Eighty percent of the world's known terrestrial plant and animal species can be found in forests, and tropical rainforests are home to more species than any other terrestrial habitat. A square kilometer of forest may be home to more than 1,000 species.

Explanation:

why should we use CER method when approaching a question

Answers

Answer:look at explanation

Explanation: you should use the CER method because you can find evidence for a question that you know is right and make a claim for the prompt, then provide a reasoning, therefore you get a full understanding of the question and so does the teacher.

How does the tectonic activity of the terrestrial planets compare with that of the gas giants?

All of the terrestrial planets have exhibited tectonic activity, but none of the gas giants have.
All of the gas giants have exhibited tectonic activity, but none of the terrestrial planets have.
All of the gas giants have exhibited tectonic activity, but only one terrestrial planet has.
All of the terrestrial planets have exhibited tectonic activity, but only one gas giant has.

Answers

Answer:

its (A). All of the terrestrial planets have exhibited tectonic activity, but none of the gas giants have.

All of the terrestrial planets exhibit tectonic activity, but none of the gas giants have.

Terrestrial planets exhibit compact surfaces, whereas gas planets don't have compact surfaces and they are composed of hydrogen (H) and helium (He).

The planets of our solar system are Mercury, Venus, Earth, Mars, Jupiter, Saturn, Uranus, Neptune.

Terrestrial planets: Mars, Earth, Venus and Mercury.

Gas planets: Neptune, Uranus, Saturn and Jupiter.

The term 'tectonic plates' refers to the pieces of the solid Earth's crust (and even the plates of other terrestrial planets). These plates exhibit a movement known as tectonic activity.

Gas planets don't have a solid surface and therefore no tectonic activity can be observed in them.

In conclusion, terrestrial planets show tectonic activity, but gas giants don't have tectonic activity.

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What is the difference between a mixture of compounds and just a compound?

Answers

Answer:

The mixture contains two or more substances mixed, but neither chemically as well as not in inexact quantity while compound includes two or more elements combined chemically and in a fixed ratio.

:)

If two events are correlated, what must be true?(1 point) One event caused the other. Both events have the same result. Both events have the same cause. The events tend to happen together

Answers

Answer:

One event caused the other  

Explanation:

A correlation refers to how related are two or more variables among each other.

Two events are correlated if the result of one of them depends on the result of the other event. Also, the probability of one of them happening will be conditioned by the occurrence of the other event.  

There are different types of correlation. When two correlated variables or events have a causal relation, this means that one of them occurred because the other one occurred first. For example, let us think about a dolphin cranium. We might measure two variables, the rostrum length and the skull length. The longer the rostrum is, the longer the skull will be. The occurrence of a longer rostrum implies a longer skull. This two events are positively correlated.    

Although not every correlated event is due to causality, whenever causality occurs between two variables, it means that they are correlated.    

 

 

Anthony Clement is applying for a car loan. The lending institution requires a personal net worth statement. Anthony currently has $1,085.00 in cash, and his camera is worth $635.00. He also owes Vista Travel $305.00 and Kelley Electronics $83.00. Complete a net worth statement for Anthony Clement. Select Current Date in the appropriate field. Assets should be listed in order of liquidity, so Cash should be listed first. Liabilities should be reported in alphabetic order.

Answers

Answer:

Net worth statement of Anthony

Assets

Current [tex]{}[/tex]                          [tex]{}[/tex]                           $1,085.00

Property, Camera (present value)    [tex]{}[/tex]          $635.00

Total Assets        [tex]{}[/tex]                                        $1,720.00  

Liabilities

Money owed others (Vista Travel)  [tex]{}[/tex]            $305.00

Money owed others (Kelly Electronics)  [tex]{}[/tex]       $83.00

Total Liabilities          [tex]{}[/tex]                                    $388.00

Net Worth                      [tex]{}[/tex]                               $1,332.00

Explanation:

A net worth statement also known as a balance sheet statement is way to present the standing of a business at a particular time. It is a record of the assets and the values of the assets as well as the business liabilities and other outstanding financial obligations the business is to settle.

3. Summarize why the definition of species had changed over time

Answers

Answer:

Originally, the distinction was based on morphological differences. However, it soon became that some types of organisms had different forms at various stages in their lives, here is why

Explanation:

changed genes is passed on to the next generation. Most mutations are bad, but some of them make the organism more successful in its life. Organisms that inherit that favorable new gene are likely to become more abundant than others of the species. (geologic times)

A pond, rich in various microorganisms, dries up due to drought. How will the amoeba in the pond protect themselves from this unfavorable condition?


Answers

Answer:

The correct answer is : by secreting a thick cell wall which encloses many amoebae in it to prevent loss of water, forming a cyst until favorable conditions emerge

Explanation:

Amoeba protects itself in unfavorable conditions such as drought or less water or food availability. Amoeba takes back all its pseudopodia and secretes a thick cell wall which form three layer hard covering arout amoeba body.

This hard covering is known as the cyst  and this process known as the encystation. Amoeba remain in this cyst form until favorable conditions emerge.

Thus, the correct answer would be - by secreting a thick cell wall which encloses many amoebae in it to prevent loss of water, forming a cyst until favorable conditions emerge

Glycolysis, formation of acetyl CoA, Krebs cycle and the electron transport chain are all involved in:

Answers

Answer:

breaking down of glucose

Explanation:

Glycolysis, formation of acetyl CoA, Krebs cycle and the electron transport chain are all involved in the breaking down of glucose, also known as respiration, in living organisms.

During glycolysis, glucose is transformed into 2 molecules of pyruvate with ATP and NADH being generated in the process.

The pyruvate molecules are each oxidized to acetyl CoA, a process that leads to the release of carbon dioxide and the formation of NADH.

The acetyl CoAs then undergo a series of reactions, combining with a 4-carbon molecule and generating ATP, FADH2, NADH, and releasing carbon dioxide. The 4-C molecule is also regenerated during the process. This is known as the Krebs cycle.

All the FADH2 and NADH made from the earlier steps then enter the electron transport chain where they donate their electrons to become FAD and NAD respectively. ATP is generated and water is formed through the reduction of oxygen.

Each DNA molecule resulting from replication has one original strand and one new strand. This also known as _________________________replication of DNA.

Answers

Answer: if ur learning about mitosis it’s prophase

Explanation:

Answer:

it is synthesis phase

Explanation:

because during synthesis phase, the cell makes an entire copy of DNA, referred to as DNA replication.

_____ is the distance between corresponding points of adjacent waves

Helllppp!!!

Answers

Wavelength is the distance between corresponding points....

Answer:

Wavelength_____ is the distance between corresponding points of adjacent waves

Explanation:

wavelength is one way of measuring the size of waves. It is the distance between two corresponding points on adjacent waves, usually measured in meters. The wavelength of a transverse wave can be measured as the distance between two adjacent crests.

What is the difference between functional anatomy and gross anatomy? (A).Functional anatomy studies the structure of organ systems; gross anatomy studies the function of organ systems. (B)Functional anatomy studies the function of the organ and organ systems; gross anatomy studies the large-scale structure of the human body. (C)Functional anatomy studies the function of organ systems; gross anatomy studies the external features of the body.

Answers

Answer:

(B)Functional anatomy studies the function of the organ and organ systems; gross anatomy studies the large-scale structure of the human body

Explanation:

Anatomy is the study of the structure of the body. Anatomical studies is important in having a better understanding of the structural characteristics and composition of a body. However, anatomy is divided into sections.

One of those parts of anatomy is GROSS anatomy, which is the study of the structures that are visible to the human eye. Hence, it is also called Macroscopic anatomy or large-scale anatomy. Functional anatomy, on the other hand, is the study of the body's structure based on their function. It is termed morphophysiology because it studies the function of organs and organ systems.

Answer:

Functional anatomy studies the structure of organ systems  gross anatomy studies the function of organ systems

Explanation:

Feathers in birds appear to have first evolved for insulation but later conveyed a new advantage in helping create light aerodynamic surfaces. This switch in function is an example of ______.

Answers

Answer:

Exaptation

Explanation:

Exaptation is the process by which the features acquire those functions for which they were not originally selected or adapted. Feather is an example of exaptation, it has appeared in various fossils of the flightless ancestors of modern birds, it later became important for flying, Once they molded for flying it adapted for that function, It is found in both behavior and anatomy.

All of the homeostatic mechanisms that monitor and adjust the composition of body fluids respond to changes in the:

Answers

Answer:

The correct answer would be - extracellular fluid.

Explanation:

Changes in extracellular fluid helps the homeostatic mechanism to monitor and regulate the composition of the body fluids. This process involves regulatory hormones such as anti diuretic and aldosterone in order to maintain body fluids.

It is easy to regulation of the extracellular fluid than trying to regulate 100 trillion cells present in Intracellular fluids as ICF have 10% more water in comparison of ECF.

Thus, the correct answer is : extracellular fluid.

A controlled scientific study examining variables is known as a
scientific ______ ?

Answers

Answer:

An experiment

Explanation:

The answer is an experiment

A controlled scientific study examining variables is known as a scientific experiment.

What is a scientific experiment?

A scientific experiment is an experiment that is done to calculate or check out a reason for scientific things to happen. It is done with variables, these variables are controlled, independent, and dependent.

Scientific experiment container hypothesis, a procedure, and it is done by proper regulated method and instruments.

Now variables can be the components whose study we are doing or the variable which affects the component.

A control scientific experiment will be controlled by the scientist and it is to be checked that what time and what face of the experiment is going on

Thus, a scientific experiment is a carefully planned, multi-variable investigation.

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(reject null/ eccept null) A decentralized organizational structure is predicated on a belief that A. decision-making authority should be put in the hands of the people closest to and most familiar with the situation, and these people should be trained to exercise good judgment. B. a command-and-control organizational scheme is the lowest cost and best way to organize the work effort. C. top executives often lack the expertise and wisdom to decide what is the wisest and best course of action. D. the best decisions emerge from a collegial, collaborative culture where decisions are made by general consensus (at least a majority vote) on what to do and when. E. organizing into work teams, having team members elect a team leader, and having team members vote on the best way to do things greatly reduces corporate bureaucracy. what is 1/16 times 1/4 as a fraction? Which option is an example of an experiment