Describe an experiment you would perform to test a proposed chloroplast-targeting signal sequence. Be specific about the type of cell and the proteins you would use. Describe the results you would expect if the sequence is indeed a chloroplast-targeting signal.

Answers

Answer 1

Answer:

I would fuse the gene encoding the protein carrying the putative chloroplast-targeting signal sequence with the gene encoding Green Fluorescence protein. The GFP-tagged target protein enable to study the expression of the putative chloroplast localization signal sequence and its final cellular localization

Explanation:

The Green Fluorescence protein (GFP) is a protein widely used in molecular biology to investigate the expression of specific genes and then track the movement of proteins in living cells. This protein also can be used as a cell marker and to identify protein-protein interactions. When GFP is excited to a given wavelength (excitation peak 395 nm), it emits green light (emission peak 509 nm), thereby the expression/localization of the GFP-target fusion constructs can be evidenced by using a fluorescence microscope.


Related Questions

remember to imagine that blood in the heart is color-coded according to its oxygenation status. What color of blood would you expect to see in isabelle's left ventricle

Answers

Answer:

Red blood.

Explanation:

Red blood is for oxygenated blood, while blue blood is for deoxygenated blood.

The blood in Isabelle's left ventricle would be red, in other words, oxygenated. The left ventricle has oxygenated blood because this blood was first in the right ventricle. Then, it went to the pulmonary arteries, which led the blood to the capillaries close to the alveoli in the lungs. In this area, the deoxygenated blood dropped the CO₂ and took O₂ becoming oxygenated blood. Now, this blood is color-coded red and will go to the pulmonary venules, then to the pulmonary vein, and from there, it will go to the left atrium. Lastly, it will go to the left ventricle to start the systemic circulation, which is the one that distributes the oxygenated blood in the body.

According to the fossil record above, during which era did mammals first appear?
A)
Cenozoic
B)
Mesozoic
0
Paleozoic
D)
Modern Day

Answers

Answer: The answer is A

Explanation:

took the quiz on usatestprep

Answer:A)

Explanation: Mammals first appeared in the Cenozoic era. My teacher explained it and it's visable in the picture.

wwhat came first the chiven or the eggg

Answers

Answer:the egg

Explanation:Eggs were around the corner before chickens even lived

Answer:

The Chicken

Explanation:

The egg could only be made by two birds that (may not have been chickens but would later evolve into chickens) mated and laid an egg.

A force of 1 N is equal to which combination of SI units?

A. 1 $2 kg/m
B. 1 m/J's
C. 1 J/m-82
D. 1 kg-m/s2

Answers

Answer:

1 kg-m/s2

Explanation:

Sharks and dolphins are not closely related but have many similar features due to the
environment where they are found. This is an example of:

A:Convergent evolution

B:Artificial selection

C:Natural selection

D:divergent evolution

Answers

It is Convergent evolution

Which of the following are reactants in the process of photosynthesis?

Answers

Answer:

Photosynthesis requires sunlight, carbon dioxide, and water as starting reactants (Figure 5.5). After the process is complete, photosynthesis releases oxygen and produces carbohydrate molecules, most commonly glucose. These sugar molecules contain the energy that living things need to survive.

Explanation:

Answer:

carbon dioxide and water

Explanation:

which of the following are reactants in the process of photosynthesis?

What two forces contribute to the creation of an ocean gyre?
The Coriolis Effect and winds.
Salinity and winds.
The Coriolis Effect and water density.
Water temperature and winds.

Answers

Answer:The Coriolis Effect and winds.

Explanation:Ocean Gyres are Circular currents in the ocean that are formed by the Earth winds and the Coriolis effect- which is caused by the rotation of the earth.

Answer:

The Coriolis Effect and winds.

Explanation:

took the quiz

If a dog has 39 chromosomes in its egg cell, how many chromosomes are in its body cell?

Answers

78
Because in the egg cell the amount of chromosomes is 1/2

If phosphofructokinase experienced a mutation that interfered with substrate binding, then what other enzyme is going to be most immediately impacted in terms of accessing substrate

Answers

Answer: Aldolase

Explanation:

In the metabolism of glucose( glycolysis) phosphofructokinase is an enzyme that catalyzes the conversation of fructose-6-phosphate into fructose-1,6-bisphosphate. This in turn is converted to pyruvate after various steps of enzymatic activity in the glycolytic pathway.

If phosphofructokinase experienced a mutation that interfered with substrate binding, the enzyme that is going to be most immediately impacted in terms of accessing substrate is the ALDOLASE.

Aldolase enzymes cleave fructose-1,6-bisphosphate to triose phosphates( glyceraldehyde 3-phosphate and dihydroxy-acetone phosphate) facilitating an increase in anaerobic production of ATP in muscle.

Therefore, the substrate for binding of aldolase, which is fructose-1,6-bisphosphate is lacking due to mutation of phosphofructokinase enzyme.

What two substances make the backbone of the DNAmolecule?

Answers

Phosphate and deoxyribose

.Complete the sentence

The addition or removal of genes from a population is referred to as_____________​

Answers

Answer:

The answer is -

Evolution

9. Doctors tested a patient to find out what molecules were in her cells. Her cells contained normal
levels of oxygen molecules, low levels of amino acid molecules, and low levels of glucose molecules
Will this patient be able to exercise normally?
a
Yes, as long as her cells have enough oxygen, her body will be able to exercise,
b
No, her cells need glucose to react with oxygen to release enough energy to exercise.
С
No, her cells need amino acids to react with oxygen to release enough energy to exercise.
d
No, her cells need glucose to react with amino acids to release enough energy to exercise.

Answers

Answer:

b. No, her cells need glucose to react with oxygen to release enough energy to exercise.

Explanation:

Living organisms are able to perform any of their day-to-day activities due to the release of energy in their individual cells. The process by which this energy is obtained by their cell is called CELLULAR RESPIRATION. Cellular respiration is the process whereby glucose (from digested food) is broken down in the presence of oxygen to yield energy in form of ATP. The general equation is as follows:

C6H12O6 (glucose) + 6O2 (oxygen) → 6CO2 + 6H2O + ATP

According to this question, a doctor is said to test a patient for the content of molecules in her cells. Her cells were found to contain normal levels of oxygen molecules, low levels of amino acid molecules, and low levels of glucose molecules. This patient will NOT BE ABLE to exercise normally because her cells need glucose in optimal amount to react with oxygen to release enough energy to exercise.

HELP ASAP!!!!
The forelimbs of mice, bats, and whales all have a similar bone structure. This is an example of which type of evidence of shared ancestry?
Question 2 options:

adapted structures

homologous structures

embryonic structures

vestigial structures

Answers

Answer:

homologous structures

Explanation:

Homologous structures are organs or skeletal elements of animals and organisms that, by virtue of their similarity, suggest their connection to a common ancestor. These structures do not have to look exactly the same, or have the same function.

Homologous structures

In the Calvin Cycle, what is ultimately made?
A.H2O
B.Carbon Dioxide
C.Oxygen
D.Glucose

Answers

Answer:

D. Glucose

Explanation:

I'm 11 ok your lucky I know that...

I need someone to answer number 9 please

Answers

the second one i’m pretty sure

Explain the Role of the Most
bucco in Living Organisms
Click the statement that best describes the role of each element in living organisms.
Carbon
Oxygen
Hydrogen
DONE

Answers

Answer:

carbon

Explanation:

A drug may cause decreased communication between neurons or between neurons and muscles or glands. What might be the common action of this drug to cause such varied effects

Answers

Answer:

acid

Explanation:

Why do you think electron microscopes allow for greater magnification than compound microscopes?

Answers

Explanation:

Electron microscopes have a greater magnifying power than optical microscopes due to the use of shorter wavelength electrons.they allow magnifications of up to one million times the size of a specimen while optical microscopes can achieve a magnification of no greater than 1000x.

I hope it's helpful!

An Electron microscope allows for greater magnification than compound microscopes it utilizes electrons of a much shorter wavelength as compared to the visible light in a compound microscope.

What is an Electron microscope?

An electron microscope may be defined as a type of microscope that utilizes a beam of accelerated electrons in order to construct or produce an enlarged image of tiny objects or specimens like virus cells.

The higher resolution power is the characteristic feature of the electron microscope. An Electron microscope allows a magnification of approximately one million times the actual size of objects or biological specimens.

While, when it comes to the compound microscope, the magnification power limits to a thousand times the actual size of objects or biological specimens.

Therefore, the utilization of electrons of a much shorter wavelength is responsible for greater magnification in the electron microscope.

To learn more about an Electron microscope, refer to the link:

https://brainly.com/question/2845212

#SPJ6

If a gene sequence in one organism is highly similar to the sequence of another gene in another organism it is called

Answers

Answer:

This question lacks options, options are: a.related. b. homologous. c protein-coding, d. comparative. The correct answer is b.

Explanation:

Homology refers to the fact in which the sequences of two or more proteins or nucleic acids are very similar due to the fact that they have the same evolutionary origin. Normally two sequences have a high similarity because they are homologous, that is, they share a common ancestor. If two sequences in an alignment are from a common ancestor, mismatches can be interpreted as point mutations (substitutions), and gaps as insertion or deletion mutations introduced into one or both lineages in the time since they diverged.

A peony plant with straight stamens and red petals was crossed with another plant having straight stamens and streaky petals. the seeds were collected and germinated, and the following offspring were obtained: 62 straight stamens, red petals 59 straight stamens, streaky petals 18 curved stamens, red petals 22 curved stamens, streaky petals. A) which allele in each pair is dominant B.) what were the genotypes of the parental plants C.) what further crosses would you make in order to get a definite answer for part a

Answers

Answer and Explanation:

Due to technical problems, you will find the complete answer and explanation in the attached files.        

Answer:

A) Straight (S) is dominant and Red (R) is dominantB) SsRr = Straight, red and Ssrr = Straight, streakyC) Straight, red = 3/16 , Straight, streaky = 3/16 ,  Incurved, red = 1/16 ,  Incurved, streaky = 1/16

Explanation:

A) Straight (S) is dominant over incurved (s)

Red (R) is dominant over streaky (r)

B) SsRr = Straight, red

Ssrr = Straight, streaky

C)                            Sr                                          sr

SR        SSRr (straight, red)                       SsRr (straight, red)

Sr         SSrr (straight, streaky)                 Ssrr (straight, streaky)

sR        SsRr (straight, red)                       ssRr (incurved, red)

sr         Ssrr (straight, streaky)           ssrr (incurved, streaky)

Straight, red = 3/16

Straight, streaky = 3/16

Incurved, red = 1/16

Incurved, streaky = 1/16

For more information on this visit

https://brainly.com/question/17756498

what molecule is taken into to start the light dependent reaction

Answers

Answer: ATP, and NADPH. These energy-carrying molecules are made in the stroma.

HELP ASAP! 50 POINTS!! WILL MARK BRAINLIEST!! THANK YOU!!
Complete the Punnett square for a dihybrid cross of a new type of cat: PpRr (mother) x ppRr (father)

Answers

Answer:

I hope this helps, if it doesn't help you please let me know, and I'll delete my answer!

Which of the following systems of the body interact when a stressful situation causes a person's heart rate to increase and pupils of the eyes to dilate?

A.
nervous system and excretory system

B.
endocrine system and nervous system

C.
respiratory system and nervous system

D.
nervous system and digestive system

Answers

Answer:

B

Explanation:

Which amphibian is the scientist most likely observing? A scientist is observing an amphibian. It has brightly colored skin, four legs, sticky pads on its feet, and a long, narrow body, a salamander because it has sticky pads on its feet O a salamander because it has a long, thin body O a frog because it has four legs O a frog because it has brightly colored skin​

Answers

Ya I think it’s letter A

Answer:

B

Explanation:

WILL GIVE BRAINLIEST WHEN POSSIBLE, STARS, EXTRA POINTS AND THANK YOU FOR THE RIGHT ANSWER!!
(Please give an explanation and no incomplete/absurd answer I really don't wanna report anybody lol)

Why might it be harder to convince people of the value of forest ecosystem services as opposed to the value of it's economic service?

Answers

Answer:

It might be harder to convince people of the value of the forest ecosystem services as opposed to the value of its economic service because truthfully, people have different ideas on what is important and what´s not. So some people may think that the forest has no worth and it doesn´t matter to them if it gets torn down or not, as long as it makes them money, but others may feel that a forest is a special place where animals and such have a place to be free. In the end, it´s always about money and morals. I hope this helps!!

Explanation:

Describe a temperature inversion.

Answers

Answer:

Temperature inversion also called thermal inversion, a reversal of the normal behavior of temperature in the troposphere ( the region of the atmosphere nearest earth's surface) in which a layer of cool air at the surface is overlain by a layer of warmer air.

Can someone help me with this please. Thank you so much

Answers

Answer:

7. SS ,ss . Ss .sS

im pretty sure the rest is 50 %

Explanation:

7. Ss, ss
8. straight nose (2), bent nose (2)
9. 50% (with capital S, so Ss)
10. 50% (Ss)
11. 50%

helppp please i honestly don’t know

Answers

Answer:

hi

Explanation:

Which part of the chloroplast MOST clearly uses modified surface-area-to-volume ratios to function efficiently?

A. thylakoid
B. outer membrane
C. stroma
D. ATP synthase

Answers

Answer:

it is c option storma that increases surface area

Answer:

the answer is A, thylakoid

Explanation:

I just took the whole unit test :)

1 point
2. The most precise type of dating used for determining a fossil's age is
relative-age dating. *
True
False

Answers

True , trust me on this, fossil tell us about are past and the environment at the time
Other Questions
Which of the following steps is NOT part of the critique process?O judgingO describingO patterningO analyzing How does daisy perceive gatsby? What tropism does the experimental data below show?A. PhototropismB. GeotropismC. Hydrotropism 7x - 2y - 1 ??????????????? Helpppppp Im giving brainlest plss Please help ASAP!How did the Founding Fathers respond to conflicts regarding a new plan for the government? Select three correct answers.-They created a system of government in which the people were not directly involved.-They developed the Constitution to address the shortcoming of the Articles of Confederation.-They developed a system where the national government had all the authority to make decisions.-They developed compromises to satisfy the concerns of different states.-They created amendments to the Constitution to address the concerns of different parties.-They promoted the ruling of a king to help establish a stable government. ASAP will give brainlist to correct answerwhich benefit does a community experience when its members have a high level of health literacy ?A - people are more likely to take risk with their health B - people are more likely to need emergency health servicesC - people are less likely to struggle with health problems D - people are less likely to practice delayed gratification A function is defined as follows:IN THE SCREENSHOTFor which x-values is f(x) = 4? Select all that apply.-21213 please help me guys... . In the equation a and b represent the price per night for a room in hotel a and hotel b respectively w week s Solve this equation.3.5(2.25 + x) = 14x = ? Would you rather lose a hand or a foot. A goat is placed in a circular pen with a radius of 15 feet. The formula for the area of a circle is A=r2, where r is the radius. If =3.14, on how many square feet of grass can the goat graze in the pen? NOBODY IS ANSWERING MY QUESTION!!!!!!!!!!!!!!!!!!!!!PLEASE HELP!!!!!!!!!!!!!!!!!!!!GIVING BRAINLIEST!!!!!!!!!!!!!!!!!!!!!!!!!!!! Guys pls help me!!!!! what type of cat is this please PLEASE HELP ASAP THIS IS DUE SOON 13. What geographic feature separated the U.S. from the war in Europe in 1939? Given m \| nmn, find the value of x. Have you ever watched Full House? Who is your favorite character and why? EXPLAIN AND ILL GIVE BRAINLIEST!