Writing Assignment So, you have learned about how the denaturation of proteins works during lecture, and you have seen examples in these videos. Now you will write a 2-3 page discussion of protein denaturation putting all that together Make sure you cover both the academic side (lecture knowledge) and practical side (what is done and observed in the lab). Make sure the answers to the following questions are contained in your report. Do NOT just answer them separately, but rather incorporate them seamlessly in your writing. This is a very broad topic, so comprehensive coverage is hardly expected, but you should demonstrate a good beginner's understanding. As usual, reference any and all resources you use.
a. What are the various levels of organization that any protein structure may have that gives it its 3-dimentional shape?Which of these changes during denaturation?
b. For each change made to a protein solution that you have seen or read about how might it affect the interactions that are involved in a protein's shape?
c. What is the difference between precipitation of a protein and its denaturation? How might you tell the difference?
d. If changing the pH of an enzyme solution greatly reduces the activity of that enzyme without precipitation, how could you tell in the lab if it were the result of denaturation or just going to a pH that is just not a good one for the enzyme? And how would a change in pH impact or even stop enzyme function without denaturation? (Consider the enzymes that are active in your stomach, such as pepsin-discussed in Wikipedia.)
e. For changes that precipitate a protein, are some more likely than others to be denaturation?
f. Etc. If you find one aspect of what you've see more interesting, go ahead and learn more and expand that section of your report and keep the other sections more basic.
Solution :
Denaturing may be defined as the process where the protein they lose their shape and their function because there is a change in the pH level or in the temperature. Although the amino acids sequence also known as primary structure, it does not change. But the shape of the protein might change a little bit so much that it can become dysfunctional in which protein is considered to be denatured.
The enzyme, Pepsin, the breakdown protein in our stomach only operates at a very low pH level. At higher pH level, the pepsin's configuration the way the polypeptide chain is being folded up in a 3 dimension begins to change. The pH level of the stomach is maintained at a low level to ensure that pepsin continues for the digest protein and that it does not denature.
There are 4 levels of the protein structure --- they are the primary, the secondary, the tertiary and the quaternary.
The primary protein is a particular sequence of amino acids in each of the polypeptide chain that makes the protein. The final protein structure depends on this sequence.
Secondary structure arise from the interactions between the neighboring or nearby the amino acids as polypeptide starts folding into 3D form.
The tertiary structure is the most important of structural levels in determining.
The quaternary structure also applies to the proteins made from more than one polypeptide chain.
What does the term "feedback inhibition” describe?
Answer:
Feedback inhibition, in enzymology, suppression of the activity of an enzyme, participating in a sequence of reactions by which a substance is synthesized, by a product of that sequence.
To approximate the actual concentration of enzymes in a bacterial cell, assume that the cell contains equal concentrations of 1,000 different enzymes in solution in the cytosol and that each protein has a molecular weight of 100,000. Assume also that the bacterial cell is a cylinder (diameter 1.0 m, height 2.0 m), that the cytosol (specific gravity 1.20) is 20% soluble protein by weight, and that the soluble protein consists entirely of enzymes. Calculate the average molar concentration of each enzyme in this hypothetical cell.
Answer:
2.4 × 10⁻⁶ M
Explanation:
Molar concentration = no of moles of solute (enzymes)/volume of solvent(cystosol)
Let V = volume of solvent = volume of cystosol = πd²h/4 where d = diameter of cell = 1.0 m and h = height of cell = 2.0 m
Concentration of enzymes, C = n/V where n = no of moles of enzymes
Also, n = m/M where m = mass of all enzymes and M = molecular weight of protein = 100,000
Note that the solvent for the enzymes is cystosol, since they are dissolved in it.
Density of cystosol,ρ = specific gravity of cystosol in g/cm³ = 1.20 g/cm³.
Now, ρ = m'/V where m = mass of cystosol and V = volume of cystosol
m' = ρV
m' = 1.20V
Given that 20 % of the cystosol is protein and all protein are enzymes, the mass of enzymes is thus m = 0.2m' = 0.2 × 1.20V = 0.24V
C = n/V
= m/MV
= 0.24V/100000V
= 2.4 × 10⁻⁶ mol/cm³
= 2.4 × 10⁻³ mol/dm³
= 2.4 × 10⁻³ M
This is the concentration of all the 1000 enzymes.
So, the concentration of one enzyme is 2.4 × 10⁻³/1000 M = 2.4 × 10⁻⁶ M
How old would this fossil be if only 1/128 of C-14 was detected in it?
Answer:
not sure but mark that guy brailest ^
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2. Which part of the nucleotide encodes (holds) the genetic information?
(highlight correct answer)
A. Sugar
B. Phosphate
C. Base
D. Sugar-phosphate backbone
Answer:
C. Base
Explanation:
The bases for DNA are adenine (A), cytosine (C), guanine (G), and thymine (T). The certain sequence gives certain genetic information.
Help please !!!!!! Need the answer asap
Answer:
i think the answer is c
Explanation:
HELP URGENT!!!!!!!!!!!
Answer:
dominant trait
Explanation:
Recessive alleles can only show up when there is no dominant allele present to suppress them.
Answer: Dominant traits
Explanation:
Recessive traits only show up when there are no dominant alleles
Extraterrestrial traits are not a thing
describe origin and evolution of early vertebrates
You discovered symptoms start to appear on your cattle and its spreading, the symptoms are swollen, fever then death.
Suppose you are a veterinary Doctor, what will you do, what procedures you will apply to examine this disease.?
Answer:
f
Explanation:
Clara, while vacationing in Italy, decided to go swimming. When she asked at what temperature the pool was kept, she was told that it had a constant temperature of 35.8°C. What temperature is the pool in °F, to the nearest tenth?
a.
53.6°F
b.
96.4°F
c.
122.0°F
d.
129.1°F
Answer:
96.4
Explanation:
The answer is at the top
When she asked at what temperature the pool was kept, she was told that it had a constant temperature of 35.8°C. The temperature is the pool in °F, to the nearest tenth is 96.4°F. Therefore, option (B) is correct.
The average kinetic energy of all of a substance's particles is used to measure temperature. The average kinetic energy of a substance rises with the speed at which its particles move, increasing its temperature and making it feel warmer.
One of the seven SI base units is the kelvin, which is the thermodynamic temperature unit. Curiously in the SI, we additionally characterize one more unit of constant temperature, called the degree Celsius (°C).
Learn more about temperature:
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The process of meiosis is essential in the sexual reproduction and life cycle of many organisms. The outcome of meiosis is haploid gametes. Which statements correctly describe the importance of meiosis to the life cycle or these organisms?
A) Increasing genetic diversity ensures that no two haploid gametes are exactly the same.
B) Taking a diploid cell to its haploid state occurs during the first cell division of meiosis.
C) Checkpoints during meiosis ensures that chromosome separation occurs accurately, avoiding non-disjunction.
D) DNA synthesis occurs before each cell division in meiosis ensures that the integrity of the chromosomes is maintained.
E) Reducing the number of chromosomes by half during meiosis ensures the chromosome number is maintained during fertilization.
Answer:
E) Reducing the number of chromosomes by half during meiosis ensures the chromosome number is maintained during fertilization.
A) Increasing genetic diversity ensures that no two haploid gametes are exactly the same.
Explanation:
Meiosis is a kind of cell division that strictly occurs in sexually-reproducing organisms. It is the process whereby four daughter cells that are genetically different from their parents are produced. In meiosis, the chromosome number of each daughter cell (gamete) is reduced by half. As highlighted in this question, the meiotic process is essential in the sexual reproduction and life cycle of many organisms.
This importance is attributed to the fact that:
- Reducing the number of chromosomes by half during meiosis ensures the chromosome number is maintained during fertilization. That is, a diploid organism (2n) produces a haploid gamete (n), which becomes diploid again when fertilized by another gamete.
- Increasing genetic diversity ensures that no two haploid gametes are exactly the same. This occurs because of a process called CROSSING OVER, which enables that the alleles of each gamete is recombined to be different from one another in order to promote genetic diversity.
Answer:
A, B, C, E
Explanation:
i just did it
Raccoons feed on dead animals. Thus, they are decomposer animals known as?
Answer:
SCAVANGER
Explanation:
Answer:
Raccoons are scavengers
A raccoon is not a decomposer but is an omnivore and scavenger. Raccoons will eat just about anything, including a wide variety of plants
What is the phenotype of the sons in generation III? How do you know? .
pulled upwards in the trunk of a tall tree Ichestue
S. Give three reasons to explain why transpiration occurs mainly through leaves
6. Explain how sunken stomata lower the rate of transpiration
11
with
ftri
Answer:
Due to stomata.
Explanation:
Transpiration occurs mainly through leaves because of the presence of stomata n the lower side of the leaves. Transpiration refers to the loss of water from the body of plants so leaves has small pores which is used by the plant to regulate internal temperature. Sunken stomata lower the rate of transpiration because it is not directly exposed to the surface which protects the escaping water vapor due to air.
A) How do scientists use molecular clocks to study evolution?
B) Explain some assumptions and limitations of the molecular clock concept.
Answer:
A) A molecular clock is a measure of evolutionary time based on the theory that specific DNA sequences mutate at constant rates. To use a molecular clock, scientists first select two different species and compare their DNA sequences. They can compare the DNA sequences directly, or by looking at the RNA and protein molecules created from the DNA. Molecular clocks help to measure the number of changes and mutations which accumulate in the gene sequence of different species over a period of time.
B) Molecular clocks assume the neutrality of gene mutation. They are based on the fact that genetic mutations although random, occur at a relatively constant rate. Evolutionary biologists use this information to deduce how species evolve and determine when two species diverged on the evolutionary time line. It is useful in the study of variations in selective forces, and also facilitates establishing the dates of phylogenetic events including those not documented by fossils. Molecular clocks do have some limitations. Rates of change may not be constant, may not be the same in all organisms, and may be different for different genes.
I used the answer above and added some stuff here and there, so credit to them. I'm not sure if what I wrote makes much sense, but hopefully it can be somewhat helpful. :)
A piece of alien DNA cannot multiply itself in the progeny cells of the organism because:
a. It has no the sequence called Origin of replication (ori) needed for starting replication.
b. A piece of DNA will be denatured and damaged soon as it has no stability.
c. A piece of DNA is easily unwound and the H bonds between nitrogen bases are broken.
d. Both A & B.
how and why does DNA change during meiosis
Answer:
When recombination occurs during meiosis, the cell's homologous chromosomes line up extremely close to one another. Then, the DNA strand within each chromosome breaks in the exact same location, leaving two free ends. Each end then crosses over into the other chromosome and forms a connection called a chiasma.
Explanation:
Key Concept Name the
main events of the cell cycle.
Why aren't there many large carnivores?
Answer:
Technically, the largest ever marine predator known thus far - the blue whale - is, for now, alive and well, but I know what you mean. You’re not talking about filter-feeders, or fish-eaters, you mean macropredators.
The last truly enormous macropredator in our oceans was probably Carcharocles megalodon, which went extinct about 3.6 million years ago. Other recent examples include the terrifying sperm whale Livyatan melvillei (which disappeared 8.9 mya) and other megatoothed sharks like C. chubutensis.
So, why did the giants go away? The answer, in truth, is likely multi-faceted. Here are some major promising hypotheses, or at least contributing factors:
Whales have declined. Giant sea carnivores like C. megalodon and Livyatan relied on marine mammals - specifically baleen whales. During the Pliocene, the Americas connected and the Central American Seaway was closed. This seems to have triggered a significant decline in the diversity of tropical whales, and would thus limit food availability for huge, energy-hungry predators.
Things got too cold. The extinction of megalodon and other giant ocean predators roughly coincides with the trend of decreasing temperatures the Pliocene brought. Giant sharks in particular, not being fully warm-blooded, would suffer from this, and it would also mean a decline in food supply. You can even see a sudden cold snap just about when Carcharocles went extinct, 3.6 mya.
Smaller predators outcompeted them. The niche of Carcharocles and the macroraptorial sperm whales is arguably still filled today - by the killer whale. Many orca pods specialize in hunting whales or other marine mammals. However, killer whales and their ancestors were perhaps more opportunistic, fast, intelligent and indeed adaptable than the massive, solitary hunters.
Ironically, the largest ever marine predator owes its existence to the demise of the largest ever marine macropredators. Since the extinction of macroraptorial sperm whales and Carcharocles megalodon, baleen whales have been gradually increasing in size, for they no longer need to be as fast and agile. Because of this, we have the privilege of sharing our world with the largest animal known to have ever lived - the blue whale.
what is the activities of life that occur the cellular level
Answer:
all the digestive activities of life (respiration , digestion, excretion, etc) occur at the cellular level.
Explanation:
-Eijiro <3
Detection of a light is
coming from a car is
and interpreting the light as
Select one:
a. Sensation perception
b. Consciousness: unconsciousness
e Bods: cons
O d. Energy: gestalt
Answer: d
Explanation:
Jadon plans on carrying out an experiment involving the burning of steel wool to illustrate the conservation of mass. Steel wool is made of iron. When it is burned it combines with oxygen to form iron(III) oxide.
Which step in his experiment is the most important if he wants his experiment to correctly demonstrate the conservation of mass?
A. The steel wool should be burned inside of a sealed jar or other closed system.
B. The steel wool should be burned over a long period of time (at least two hours).
C. The steel wool should be burned in a room that is at room temperature (20–25°C).
D. The steel wool should be burned in its entirety so that no iron is left in its original state.
Answer:
1) Jadon plans on carrying out an experiment involving the burning of steel wool to illustrate the conservation of mass. Steel wool is made of iron. When it is burned it combines with oxygen to form iron(III) oxide.
Which step in his experiment is the most important if he wants his experiment to correctly demonstrate the conservation of mass?
A.
The steel wool should be burned inside of a sealed jar or other closed system.
Explanation:
The explanation for number 1 is in the link below and the answer is provided also:
https://quizizz.com/admin/quiz/5dee60258f1145001bc02ed5/physical-science-2nd-9-weeks-practice-test-2020
what enzyme helps digest lipids?
- lactase
- amylase
- cellulase
- lipase
Answer:
The answer is lipase
Hope this help
Explanation:I did the test
What is the argument against taking persistent vegetative state patients off of life support?
Answer:
An argument could be that there have been many instances where people on life support or in a vegatative state have 'woken' up and gotten better after many years. So, taking someone off life saving machines could be taking away their chance of getting better and their chance of surviving. Since the person is not dead, some may even see it as murder.
Explanation:
umm I tried <3
Which of the following scientists discoverell that in DNA there is the same amount of 1 point
adenine as thymine and the same amount of guanine as cytosine?
Answer:Erwin Chargaff
Explanation:
why is the structure of dna important to its function
Answer:
The function of DNA is tied to its structure. ... The sugars and phosphates link the nucleotides together to form each strand of DNA. When two strands of DNA come together, base pairs form between the nucleotides of each strand. Nitrogenous bases pair together in the following way: A-T and C-G.
Explain how an abiotic factor (stronger storms, warmer oceans, or lower ocean pH) can affect populations in a coral reef ecosystem
Answer:
Climate change leads to: A warming ocean: causes thermal stress that contributes to coral bleaching and infectious disease.
Explanation:
Sea level rise: may lead to increases in sedimentation for reefs located near land-based sources of sediment. Sedimentation runoff can lead to the smothering of coral.
The original source of the matter in food
Answer:
I think your asking what is the original source of the matter in food
Explanation:
if so The Sun is the major source of energy for organisms and the ecosystems of which they are a part.
Polydactyly is expressed when an individual has extra fingers and/or toes. Assume that a man with six fingers on each hand and six toes on each foot marries a woman with a normal number of digits. Having extra digits is caused by a dominant allele. The couple has a son with normal hands and feet, but the couple's second child has extra digits. What is the probability that their next child will have polydactyly
Answer:
The probability of the couple´s next child having polydactyly is 1/2 = 50%
Explanation:
Available data:
Due to technical problems, you will find the complete answer and explanation in the attached files
Which of the following is carried out by vesicles?
O Synthesizing proteins
O Facilitated diffusion
Breaking down substances within the cell
Organizing organelles prior to division
Answer:
breaking down substances within the cell
Explanation:
there are many different types of vesicles, Peroxisomes and lysosomes are both types that break down substances in the cell
The correct statement about vesicles is "Breaking down substances within the cell"
Vesicles are an organelle isolated from the cytoplasm by a membrane-like lipid layer.
Its function is to store, move and process products and cellular waste.Endocytosis and exocytosis transports are characterized by the fact that the substances move through vesicles.The vesicles incorporate substances (endocytosis) and through degradation, they expel large particles that cannot cross the plasma membrane, this mechanism is exocytosis.Therefore, we can conclude that the cell incorporates, inside the vesicle, particles from the extracellular environment and also through the vesicles it releases degradation products to the extracellular environment.
Learn more about the vesicles here: https://brainly.com/question/11131709