A 50 mL sample of soup was found to be contaminated with E. coli. A dilution of the soup was prepared by adding 14 mL of the soup to 20 mL of water. This was then diluted 1/60, and finally 10-3. Then .25 was added in to 2 plates. The first plate had a colony count of 72 and second had 65.


To do the dilution series does the 50 need to be added?

Answers

Answer 1

Answer:

The answer is "[tex]\bold{4.0 \times 10^7 \ \frac{CFU}{mL}}[/tex]"

Explanation:

The very first thing we must do is figure out where the plate was really a countable sheet.  By default, at least 30 and 300 colonists must have been on a computable plate. It is deemed incorrect beyond and below this range.  We also will pick the studded 0.25 size, leading towards 72 and 65 colonies. Now calculate the double average.

[tex]\to 72+ \frac{65}{2} = 68.5[/tex]

We need to calculate its dilution factor for both the second item. Increase for all of this  

All of the dilutions you also made:

[tex]\to \frac{7}{17} \times \frac{1}{60} \times \frac{1}{1000} \\\\= \frac{7}{1020000}\\\\= 6.8 \times 10^{-6}[/tex]

[tex]\to \frac{14 \ ml \ soup}{14 \ ml} + 20\ mL\\\\[/tex]

[tex]\to \frac{CFU}{mL} = \frac{Colonial \ number}{Dilution \ Factor \times Volume \ plated}\\\\[/tex]

            [tex]= \frac{68.5}{6.8 \times 10^{-6} \times 0.25 \ mL} \\\\ = 4.0 \times 10^7[/tex]


Related Questions

How will agents of weathering such as wind and water most likely affect this landscape

Answers

Answer:

Answer

Explanation:

The wind and water can break down the landscape and affect its physical formation. Water for example can break down rocks and make a ditch from 1 foot deep to 6 feet deep.

Exit Ticket 11/16 Biology TEKS 6A #3
Which of the following most likely influences the sequence of sub units in a protein?
O The enzymes produced by the cell
The type of food that fuels the cell
The materials in the cell membrane
O The DNA in the chromosomes of a cell

Answers

Answer:

The enzymes

Explanation:

If the haploid number of an organism is 32, how many pairs of chromosomes would be found in each of the cells of the adult organism?

1. 128
2. 16
3. 32
4. 64

Answers

64 is the answer because you would double 32 hope it helps.

In '57 Chevy's, the gene for having fuzzy dice (F) on the rear view mirror is dominant
over the gene for having smooth dice (f) on the rear view mirror. The genotype of a
smooth dice is -
FF
heterozygous
ff
cannot be determined

Answers

Answer:

ez i just took the test is FF pls give brainly

Explanation:

According to the scenario, the genotype of a smooth dice is ff. Thus, the correct option for this question is C.

What is a Genotype?

A genotype may be defined as the ultimate combinations of alleles of those genes which are significantly taken for specific studies in order to determine some facts and knowledge.

According to the context of this question, it is clearly given that the gene for having fuzzy dice (F) on the rearview mirror is dominant over the gene for having smooth dice (f) on the rearview mirror. This means that F>f. So, it the phenotype of a recessive allele has to visualize in any organism, its genotype must carry form forms of the recessive allele.

Therefore, according to the scenario, the genotype of a smooth dice is ff. Thus, the correct option for this question is C.

To learn more about Genotypes and phenotypes, refer to the link:

https://brainly.com/question/902712

#SPJ2

What stage of mitosis involves replicated chromosomes separating and then non- replicated chromosomes migrating to opposite ends of the newly forming cells? metaphase anaphase telophase prophase​

Answers

Answer:

anaphase

Explanation:

the image below is anaphase

Photosynthesis occurs both in plants and animals true or false

Answers

Answer:

false!

Explanation:

it only occurs in plants <3

8 facts about the Geosphere

Answers

1 geosphere is the earth's solid rock, 2Without the geosphere it wouldn’t be possible for humans to live on any planet because there would be no solid hard ground.
3The geosphere is 4.6 billion years old.
4Piling up our waste in landfills affects the geosphere. Yeah I know we are terrible...
5 the geosphere includes rocks and minerals
6 with no geosphere there wouldn’t be lakes rivers etc...
7 the geosphere is with a radius of 6,357 kilometers
8 we all need the geosphere to live
Hope this helped!!!

Facts:

The Geosphere is basically the insides of the earth so breaking it up into eight parts will be based on each section of it.

1. The crust is the top layer of the Earth, it consists of the water and land we see. It is a shell that lays atop or covers the Upper mantle piece.

2. The mantle is a layer between the crust and core, Earth's mantle is a rocky shell that is roughly 2,900km thick and is 84% of Earth's volume.

3. The Geosphere is the solid part of the Earth that consists of rock, soils and sediments on the surface of the Earth.

4. The core is the innermost layer of the Earth and is up to 6371km from the surface of the Earth, it consists of Nickel and Iron and are seen as main components of it.

5. The outside of the core is known as the molten core due to it mainly being liquid metal from the heat and the inner portion of the core known as the solid core.

6. Another name or term given to the Geosphere is called the lithosphere, depending on the definition they both mean the same thing but are still argued by scientists.

7. The Geosphere is in constant motion due to the tectonic plates. These plates are what causes earthquakes ,volcanoes to erupt and even tsunamis.

8.There are eight major tectonic plates making up the earth's geosphere. They are constantly moving, but usually only a few centimeters each year, this is known as continental drift.

How do the nutrients in the food you eat reach the cells in your body?​

Answers

Facillitated Diffusion

Which of these is a possible description of the offspring? some flowers with white petals and some with light blue petals some flowers with dark blue petals and some with white petals all flowers with light blue petals all flowers with some dark blue petals and some white petals

Answers

Answer:

All flowers with some dark blue petals and white petals.

Explanation:

Answer:

ANSWER CHOICE IS D

Explanation:

correct on the edg test

An ecosystem is best described as the

A) Type of food that an organism eats
B) Type of home an organism builds
C) Group of organisms in a particular place
D) Living and nonliving factors in one place

Answers

I think it’s C) Group of organisms in a particular place

What did Darwin believe?
A.
Darwin believed that all organisms have a common ancestor.
OB.
Darwin believed that all organisms were created simultaneously by the force of nature.
C.
Darwin believed that all organisms have descended from two different ancestors.

Answers

Answer:

Darwin's theory of evolution declared that species survived through a process called "natural selection," where those that successfully adapted or evolved to meet the changing requirements of their natural habitat thrived and reproduced, while those species that failed to evolve and reproduce died off.

Explanation:

i looked it up on google

Answer:

The correct answer is option B-Darwin believed that all organisms were created simultaneously by the force of nature.

Explanation:

Hi. Nice to meet you and have a great day!

Regional erosion occurs at a rate of 2 m per 1,000 years.
How much regional erosion will occur over 100,000 years?
A) 20 m
B) 200 m
C) 2,000 m
D) 100,000 m

Answers

Answer:200m


Explanation: If we do the math here we will see that they multiplied 1,000 by 100 to get 100,000 so we will multiply the 2 by 100 and we will get 200.

Answer:

B

Explanation:

For psychologists, controlling electronic information is important because electronic information is __________.

Answers

B.
trivially easy to copy and redistribute

Answer:  B trivially easy to copy and redistribute

Explanation:

Which statements about light microscopes are correct?

1. To calculate the magnification of a light microscope the eyepiece lens and objective lens magnifications are added together.

2. As the magnification increases the resolution decreases.

3. The resolution of a light microscope is limited by the wavelength of light.

4. The scale on a stage micrometer is resolved more clearly than an eyepiece graticule.


1,2,3 and 4
1,2,3 and 4

1,3, and 4 only
1,3, and 4 only

2 and 3 only
2 and 3 only

2 and 4 only
2 and 4 only

Answers

2 and 3 only is the correct answer ! Hope this helped

C. 2 and 3 only

Light Microscope:The light microscope is an instrument for visualizing fine detail of an object. It does this by creating a magnified image through the use of a series of glass lenses, which first focus a beam of light onto or through an object, and convex objective lenses to enlarge the image formed.The resolution of the light microscope cannot be small than the half of the wavelength of the visible light, which is 0.4-0.7 µm. The resolution of a light microscope is limited by the wavelength of light. Resolution is a somewhat subjective value in optical microscopy because at high magnification an image may appear unsharp but still be resolved to the maximum ability of the objective.

Thus, statements 3 and 4 are correct. Therefore option C is right choice.

Learn more:

brainly.com/question/12347213

what is the similarities between eukaryotic and prokaryotic​

Answers

Both have a  plasma membrane, cytoplasm, and ribosomes.

Please see image for references.

Answer:

Eukaryotic cells have a defined nucleus in a spherical shape, while prokaryotes have a nucleus in a ring shape, an example of a eukaryotic cell is the animal and plant cell, and an example of a prokaryotic cell is a bacterium.

uwu

Hope I help you!

Many people die whilst they are waiting for an organ transplant. Why is this?

Answers

Answer:

because the organs they need are not donated in time!!!

What evolutionary advantage might on/off signaling have over this continuously variable kind of signaling?

Answers

Answer:

Hello the options to your question is missing below are the missing options

A threshold to cause a signal is lower than in graded potentials. Graded potentials can cause a strong but single depolarization signal.

The speed of propagation is much faster as depolarization in one location causes depolarization in the neighboring ones.

Propagation of the on/off signals requires less ATPATP because the neighboring locations can depolarize each other.

The on/off signal may be propagated over long distances without any decay. Therefore, the signal is reliable.

The power of the stimulus does not matter once it triggers the signal. Therefore, different stimuli may cause the desired response.

Answer :The speed of propagation is much faster as depolarization in one location causes depolarization in the neighboring ones.

Propagation of the on/off signals requires less ATPATP because the neighboring locations can depolarize each other.

Explanation:

Some of the evolutionary advantage that on/off signaling have over this continuously variable kind of signaling are :

The speed of propagation is much faster as depolarization in one location causes depolarization in the neighboring ones.Propagation of the on/off signals requires less ATPATP because the neighboring locations can depolarize each other.

What can a fertilized egg grow into?
A. a heart-shaped plant
B. a mature fern

Answers

A Mature fern. Hope this helps

what is a lignite



plz help me ​

Answers

Answer:

lignite is a dark brown to black combustible mineral formed over Million of year by the partial decomposition of plant.

Explanation:

a soft brown coal that provides low-cost energy.

Hope this helps

What is the function of the testes? There is more than one answer.

A. Make estrogen

B. houses the developing fetus

C. houses the developing sperm

D. make testosterone

E. is the site of oogenesis

F. the site of spermatogenesis

Answers

Answer:

D

Explanation:

function of teses is to make testosterone

C, D, and F... hope that helps!

A geologist concludes that a rock sample is an extensive igneous rock. Based on this information, which statement about the rock is accurate? A. The rock likely came from a pluton B. The rock formed from cooling lava C. The rock cooled slowly over millions of years D. The rock formed within earth's crust

Answers

B I’m pretty sure if not it’s d But I always say go with ur gut but B

If the last pair reflects whether the organism is a male or female, which would the organism be?

Answers

Complete question:

There is a karyotype in this question, which is missing. You will find it in the attached files.

Answer:

The individual is a male.

Explanation:

In mammals, sex is determined by a pair of sexual chromosomes. Sexual chromosomes in humans and other mammals are X and Y. The females are represented by the combination of two X chromosomes, while males are represented by the combination of an X chromosome with a Y chromosome. This is, females are XX, and males are XY. When looking at the sexual pair of chromosomes in a karyotype, you can figure out which is the individual´s gender by comparing their sizes. The X chromosome is much bigger and longer than the Y chromosome.

In the shown karyotype, the last pair represents the sexual chromosomes. You can see that one of them (the one on the left) is long and express a sort of J shape, while the other chromosome (on the right) is very short and small. According to what we previously mentioned, the X chromosome is the long one on the left, and the Y chromosome is the short one on the right. This is a male individual.

Sex chromosomes are the central determinant that defines the sex of an individual. By analyzing the karyotype one can conclude the sex chromosomes and the autosomes.

The correct answer is:

The individual is male.

Reason for the answer:

In organisms like mammals, the sex of an individual is determined by sex chromosomes. Humans and many other mammals have two sex chromosomes X and Y.

The females have two X chromosomes(XX), while males have one X and one Y chromosome (XY).

By studying karyotype one can distinguish among the X and Y chromosomes. The X chromosome is bigger than the Y chromosome.

The karyotype provided in the question represents sexual chromosomes. The chromosome present on the left is large and is J-shaped which represents the X chromosome, while the chromosome present on the right is small and depicts the Y chromosome.

Therefore, the given Chromosome is the Y chromosome and represents a male chromosome.

To learn more about sex chromosome follow the given link:

https://brainly.com/question/19426394

Where in the cell does the process of cellular respiration take place?

Answers

Answer:

It begins in the cytoplasms of the cells ! It then moves to the mitochondria

HELP ME PLEASE PLEASE HELP IM BEGGING YOU​

Answers

Answer:

C

Explanation:

The hot air and cold air mix making it updraft and downdraft

(ILL BE GIVING BRAINLIEST IF U HELP!! :D)

Answers

Answer:

I am pretty sure Stella because notice that she is doing the same experiment again and again.

Answer:

stella

Explanation:

10. Which statement best describes what happens when an enzyme and a substrate
come together?

Answers

Answer:

The substrate fits in the enzyme

Explanation:

Hope this helped

Answer: c

Explanation:

Section 2: Conduct internet research on your selected ecosystem to help you generate a list of three criteria and two constraints. Your criteria and constraints should consider relevant factors to the problem, such as costs, reliability, safety (to humans and wildlife), human needs, environmental impact, local biodiversity, and the aesthetics of the area. List the criteria in order of most to least important and assign each to a related sub-problem. In the rationale column, explain why you placed your criteria in the order you selected.

Answers

Answer:

Constraints:

Funding

Protective equipment

Research

Installation

Criteria

Fertilizers

Rationale:

This is a big deal being that fertilizers are needed to help plants experts are still looking for ways to knock down the amount used.

Which Sub-Problems does this criteria address?

The main environmental problem associated with fertilizer use is contamination of water with nitrates and phosphates. The nitrogen from fertilizers and manures are eventually converted by bacteria in the soil to nitrates. ... High nitrate levels in drinking water are considered to be dangerous to human health.

Criteria:

Human Waste

Rationale:

Human waste is a large problem and the government is trying to stop the public from disposing of it.

Which Sub-Problems does this criteria address?:

Globally, almost 80 percent of the world's wastewater flows back into the ecosystem without proper treatment. This means that almost a quarter of people drink water contaminated with human waste, according to United Nations Water. That incubates deadly diseases, including cholera, dysentery, typhoid and polio.

Criteria:

Oil  

Rationale:

Oil is needed for just about everything but it is very costly when it comes to the environment.

Which Sub-Problems does this criteria address?

Oil spills have a devastating effect on the environment, ruining habitats and killing the organisms that live there by sticking to them, destroying their food sources and poisoning them. Additionally, oil hurts the economy by harming the fishing industry, as well as other trades that rely on the ocean.

Explanation:

1. Blank are the simplest forms of life.​

Answers

Answer:

A nanobe is a tiny filamental structure first found in some rocks and sediments. Some scientists hypothesize that nanobes are the smallest form of life, 1/10 the size of the smallest known bacteria.

How does a sperm cell get to an egg?
A. It travels through
water.

B. It is carried by a
pollinator.

Answers

Answer:

B. It is carrie6 by a pollinator

Your answer is B. The sperm does not travel through water.

Can a bearded dragons eat a Hammerhead worm.

Answers

Answer:

Eww what is that in the first place. But if that thing is not posinond i think people and dragons can eat it.

Explanation:

Answer:

no it looks to big for a bearded dragon even an adult

Explanation:

Other Questions
Agriculture, one of the oldest economic activities, is a good indicator of cultural diversity. How does agriculture bring out cultural diversity of the world? (2 points) Why does TJC differentiate between critical access hospitals (CAHs) and regular hospitals? A. CAHs are long-term care facilities that patients live at for months at a time, making their safety considerations more extensive than hospitals'. B. CAHs provide specialized, extensive treatment, making their safety considerations more extensive than hospitals'. C. CAHs only treat patients during normal business hours and don't keep patients overnight, making their safety considerations more limited than hospitals: D. CAHs are smaller and only keep patients for up to four days, making their safety considerations more limited than hospitals'. 30 POINTS!! PLEASE HELP ME! THANK YOU SO MUCH!!show all steps to solve these problems in the photos An ecosystem is best described as theA) Type of food that an organism eats B) Type of home an organism buildsC) Group of organisms in a particular place D) Living and nonliving factors in one place Give three examples of monoatomic molecules. Read and choose the option with the correct word or words to complete the sentence.Laura y Pilar, necesitan la computadora?No, tenemos libros, el cuaderno y un bolgrafo. Gracias.Laura y Pila tienen que ________. tomar la foto poner el instrumento jugar al juego hacer la tarea Why are the techniques of metaphor and simile only rarely used in speeches? A. They are considered too fanciful. B. They are only for very educated people. C. They are only for people who know a lot about literature. D. They are too confusing. how did geographic features affect the settlement in africa weeeeeeeeeeeeeeeelp What is an expression for 2 over 3 (4x +9). Fire can technically reproduce. However, it is not considered a living thingbecause...OFire does not use energyO Fire does not respond to its environmentO Fire is not made of cellsO Fire does not grow Greenhouse gases in the atmosphere trapA.solar energy leaving Earth.B.infrared energy leaving the sun.C.solar energy near Earth's surfaces.D.infrared radiation leaving Earth. On a sunny day, a 4-foot red kangaroo casts a shadow that is 5 feet long. The shadow of a nearbyeucalyptus tree is 20 feet long. Find the height of the tree? Wingspans of adult horses have approximate normal distribution with mean 125 cm and standard deviation 12 cm. What are the rst and third quartiles of the wingspans What does Jill tell le chevalier about her brother?Il s'est brl.Il s'est cass la jambe.Il a la grippe.Il est en prison. Determine the number of moles of Sulfur in 3.73 x 1035 atoms of S? Why were the Native Americans so important in the clash between the French and British? Who had the advantage with the Native Americans and why? Which of the following expressions are equivalent to 18x + 12 - 4? select three that apply.A. 26xB. 30x-4C. 18x+8D. 2(9x+4)E. 2(9x+12 - 4F. 6(3x+2)-4 5. You require a high-current battery to start a large tractor. While shoppingfor this battery, should you be more concerned with the battery's ratingof volts or amps? Explain. Which of the following expressions is this one equivalent to?2x? - 15x + 17x+3X 5x?- 2x+5+O A.+27x - 52x - 5x-8 - 37B.X-52x2 25x 108 - 321X - 5D.2x 5 3X-5